Patents Examined by Stephen Chong
  • Patent number: 9909100
    Abstract: This invention relates to a culture medium for cultivation of Leishmania promastigotes, comprising inorganic salts, organic constituents and peptone/hydrolystate and at least one plant extract.
    Type: Grant
    Filed: April 25, 2014
    Date of Patent: March 6, 2018
    Assignee: Indian Council of Medical Research
    Inventors: Pradeep Das, Anil Kumar Gupta
  • Patent number: 9890397
    Abstract: A hydrogen peroxide production method, system, and apparatus is provided for producing large volumes of hydrogen peroxide having concentrations up to and excess of 80% in one continuous cycle. In one aspect, the method can include mixing NQO1 enzyme, an NQO1 activated compound or molecule, and an NADH or NADPH cofactor with an aqueous solution, dispensing the aqueous solution within or onto a semi-permeable membrane, wherein the semi-permeable membrane further includes a pre-defined molecular weight barrier. In addition, an oxidation-reduction reaction of the NQO1 enzyme, the NQO1 activated compound or molecule, and the NADH or NADPH cofactor within the aqueous solution produce hydrogen peroxide at a concentration level. Here, the produced hydrogen peroxide is above the pre-defined molecular weight barrier of the semi-permeable membrane and diffuses through the semi-permeable membrane to be extracted for use.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: February 13, 2018
    Inventor: Gaurab Chakrabarti
  • Patent number: 9862921
    Abstract: There is provided a method for noninvasively evaluating the cell state (proliferation, multi-layering, and differentiation) of a cell sheet as a mimic tissue at the time of culturing the cell sheet. The method is characterized in that an analysis of an amino acid is conducted with the use of the culture supernatant of a cell sheet to monitor a change in the concentration of any amino acid selected from a group of 5-species of amino acids (Ile, Val, Ser, Leu, and Ala), thereby making a determination.
    Type: Grant
    Filed: June 17, 2014
    Date of Patent: January 9, 2018
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Naoko Senda, Yoko Inoue, Hiroaki Nakagawa, Akihiro Shimase, Ryota Nakajima, Kazumichi Imai, Shizu Takeda
  • Patent number: 9856447
    Abstract: Methods and apparatus for culturing microorganisms are described, including culturing in mixotrophic culture conditions. A bioreactor array with multiple culture vessels with independently controllable inputs is used to culture similar cultures of microorganisms in which at least one parameter differs from other culture vessels in the bioreactor array.
    Type: Grant
    Filed: July 21, 2015
    Date of Patent: January 2, 2018
    Inventors: Anna Lee Tonkovich, Thomas Adame, Shan Qin, Eneko Ganuza Taberna
  • Patent number: 9855296
    Abstract: The invention features methods, kits, and compositions for mitochondrial replacement in the treatment of disorders arising from mitochondrial dysfunction. The invention also features methods of diagnosing neuropsychiatric (e.g., bipolar disorder) and neurodegenerative disorders based on mitochondrial structural abnormalities.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: January 2, 2018
    Assignee: The McLean Hospital Corporation
    Inventors: Anne M. Cataldo, Bruce M. Cohen
  • Patent number: 9839657
    Abstract: Disclosed herein are compositions that comprise one or more types of bacteriophage and methods of using such bacteriophages as a prebiotic to promote the growth of beneficial bacteria by decreasing harmful bacterial populations and releasing nutrients into the environment for good bacteria in the digestive system of an individual.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: December 12, 2017
    Inventors: John Deaton, Elizabeth Ertle, Hilton Grant Dawson
  • Patent number: 9840535
    Abstract: Disclosed is a method of recovering a peptide, including: mixing a liquid sample containing a complex of peptide and protein in blood with a reagent containing a neutral amino acid, an acidic amino acid or both the neutral and acidic amino acids to liberate the peptide from the protein in blood; and recovering the liberated peptide.
    Type: Grant
    Filed: January 14, 2015
    Date of Patent: December 12, 2017
    Inventor: Kana Kawasaki
  • Patent number: 9840692
    Abstract: Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.
    Type: Grant
    Filed: June 24, 2014
    Date of Patent: December 12, 2017
    Assignee: Wilson Wolf Manufacturing
    Inventors: Daniel P. Welch, John R. Wilson
  • Patent number: 9833473
    Abstract: Provided are the tolerogenic dendritic cells for treating a myocardial infarction and a method for preparing the same, and more particularly, the tolerogenic dendritic cells for treating a myocardial infarction, which can treat cardiac insufficiency that occurs by excessive remodeling of left ventricle in the heart muscle recovery process after an acute myocardial infarction, and a method for preparing the tolergenic dendritic cells. According to the present invention, the inflammatory reaction and the excessive remodeling of left ventricle in the heart muscle recovery process after a myocardial infarction can be inhibited, and thus, the incidence rate of cardiac insufficiency can be significantly reduced, thereby being effectively used for treating a myocardial infarction.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: December 5, 2017
    Inventors: Ki-Yuk Chang, Eun-Ho Choo, Hyo-Eun Park, Eun-Hye Park, Dae-Seog Lim, Jun-Ho Lee
  • Patent number: 9797855
    Abstract: A method for the determination of pollen viability and/or maturation grade of a pollen population, comprising mechanically removing pollen grains from flowers; re-suspending pollen grains in an electrically conductive buffer for keeping the plant cells to remain viable; passing the pollen suspension through an appropriate filter with a pore size suitable for a microfluidic device being adapted to perform impedance flow cytometry (IFC); An advantage of the present invention is that a plant cell does not have to be stained for viability analysis, the method is non-invasive and a high number of cells can be analyzed in a short time frame in real-time and on-site. It is possible to follow developmental processes of plant cells, and that it is applicable to all plant species. In particular, the method allows a standardized measurement independent of the location and all over the world.
    Type: Grant
    Filed: January 31, 2015
    Date of Patent: October 24, 2017
    Assignee: Amphasys AG
    Inventors: Iris Heidmann, Marco Di Berardino, Kim Kok, Grit Schade-Kampmann
  • Patent number: 9790484
    Abstract: The present invention relates to compositions for encapsulation of biomaterials in a silica-matrix. The present invention includes a composition for formation of a silica-matrix encapsulated biomaterial. The composition includes a reactive silicon compound and a biomaterial with a catalytic activity. When encapsulated in the silica-matrix, the biomaterial at least partially retains its catalytic activity. The present invention also relates to methods of making silica-matrix encapsulated biomaterials, and to methods of using silica-matrix encapsulated biomaterials, including methods of treating water or gas using the silica-matrix encapsulated biomaterials.
    Type: Grant
    Filed: February 22, 2012
    Date of Patent: October 17, 2017
    Assignee: Regents of the University of Minnesota
    Inventors: Lawrence P. Wackett, Alptekin Aksan, Michael J Sadowsky, Eduardo Reategui, Lisa Kasinkas
  • Patent number: 9725585
    Abstract: The invention relates to the use of branched maltodextrins for inducing lysis of the cell walls of eukaryotic organisms having a polysaccharide wall in the lumen of the intestine of an omnivorous or carnivorous animal comprising an intestinal flora and also for synergistically increasing the effect of the branched maltodextrins in the induction of the growth of the intestinal flora of an omnivorous or carnivorous animal. The invention also relates to the composition intended for this use and to a method for improving health or for food supplementation.
    Type: Grant
    Filed: July 20, 2009
    Date of Patent: August 8, 2017
    Assignee: Roquette Freres
    Inventors: Laetitia Deremaux, Daniel Wils
  • Patent number: 9719144
    Abstract: The present disclosure provides for characterization of normal flora and identifying biomarkers in the gut of healthy, neruotypical subjects. Aspect of the disclosure provide for the characterization of the gut microbiome in ADS subjects, characterized by reduced richness and significant loss of the ‘Prevotella-like enterotype’ compared to neurotypical subjects. The relative abundance of genera Prevotella, Coprococcus, Prevotellaceae and Veillonellaceae are significantly lower in autistic children than in neurotypical children. Further, Prevotella, is one of the three main classifiers for the human enterotypes, along with Bacteroides and Ruminococcus. These three core genera are among main contributors in the principle component analysis. ‘Prevotella-like enterotype’ was absent in the autistic group, while neurotypical samples showed an even distribution among the three enterotypes.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: August 1, 2017
    Inventors: Rosa Krajmalnik-Brown, Dae-Wook Kang, Jin Gyoon Park, Joshua Labaer, Zehra Ilhan
  • Patent number: 9717821
    Abstract: The present invention relates to novel formulations comprising a dry powder fibrin sealant comprised of a mixture of fibrinogen and/or thrombin, for use in the treatment of wounds or injuries, in particular for use as a topical hemostatic composition or for surgical intervention.
    Type: Grant
    Filed: July 6, 2012
    Date of Patent: August 1, 2017
    Assignee: Mallinckrodt Pharma IP Trading D.A.C.
    Inventors: Eliane Schutte, Linda Zuckerman, Richard Senderoff, Glen Martyn
  • Patent number: 9689011
    Abstract: The invention relates to a method for the production of a solid bio-product wherein at least 80% of the original indigestible oligosaccharide (raffinose, stachyose and verbascose) content has been degraded into digestible mono- and disaccharides, comprising the following steps: 1) providing a mixture of milled or flaked or otherwise disintegrated biomass, comprising oligosaccharides and optionally polysaccharides and further comprising proteinaceous plant parts, water and one or more enzyme preparations containing ?-galactosidase(s); 2) reacting the mixture resulting from step (1) under continuous mixing and under conditions where the water content in the initial mixture does not exceed 65% by weight, for 0.15-36 hours at a temperature of about 20-65° C.; 3) incubating the reacted mixture from step (2) at a temperature and in a time period which inactivate said ?-galactosidase(s), as well as solid bio-products obtainable by such method.
    Type: Grant
    Filed: May 15, 2013
    Date of Patent: June 27, 2017
    Assignee: HAMLET PROTEIN A/S
    Inventors: Katrine Hvid Ellegård, Karl Kristian Thomsen, Ole Kaae Hansen
  • Patent number: 9677044
    Abstract: In a method for picking up cell material from a culture dish a picking tool is displaced towards the cell material. Contact between the tool and the cell material is determined by carrying out capacitive measurements. After contact the tool is removed from the cell material. In an assembly for performing said method a support made of electrically conducting material supports the dish. A measurement device determines the start electrical capacity of the system composed of picking tool and support in a starting position of the tool, and determines the electrical capacity of the system when lowering the tool towards the dish. The measurement device provides signals representing the electrical capacity to a controller, which comprises a comparator for performing a comparison of the electrical capacity during lowering of the tool with the start electrical capacity. The controller controls the positioning of the tool at least based on this comparison.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: June 13, 2017
    Assignee: BD Keistra B.V.
    Inventors: Jetze Botma, Martijn Kleefstra, Martijn Xander Berntsen, Tino Walter Van Der Zee
  • Patent number: 9670454
    Abstract: Methods of inducing or increasing lipid and protein production in microalgae are disclosed. Methods of enhancing microalgae biomass are also disclosed. The methods may comprise inoculating a culture media with microalgae and propagating the microalgae under heterotrophic growth conditions. The heterotrophic growth conditions may comprise inhibiting exposure of the inoculated culture media to light. The methods may further comprise delivering oxygen into the inoculated culture media. Additionally, lipids, proteins, and other cellular components may be isolated and purified from microalgae cultivated under the disclosed methods.
    Type: Grant
    Filed: August 8, 2016
    Date of Patent: June 6, 2017
    Assignee: Entech, LLC
    Inventors: John Whitman, Robert G. Barton, Michael Ripka, III, Michael Ripka, Jr., Robert L. Harris
  • Patent number: 9663752
    Abstract: A generally cylindrical collapsible mixing vessel, comprising: a) a flexible plastic film side wall, a top wall and a bottom wall, wherein both the top wall and the bottom wall are attached to the side wall and the side, top and bottom walls define an inner volume of the vessel; b) an agitator located in the inner volume and; c) at least one vortex breaker made from flexible plastic film, located in the inner volume and attached to the bottom wall and/or the side wall via string elements, wherein said vortex breaker extends along the side wall and inwards in a generally radial direction.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: May 30, 2017
    Inventors: Klaus Heinrich Gebauer, Rolf Axel Hjorth, David Ronnholm
  • Patent number: 9642354
    Abstract: The invention discloses a multi-layer cell freezing vial and method of obtention thereof. The method of obtention is a cell freezing and thawing process that avoids the need of incorporating new fresh medium to the thawed cells for the dilution of the cryoprotective agent. This is achieved by the previous freezing of an extra layer of culture medium in addition to the frozen cell solution containing said cryoprotective agent. At the time of thawing, the culture medium and the cell solution mix themselves, turning the concentration of said cryoprotective agent to a non-toxic dilution and achieving the effective exclusion of said cryoprotective agent from the living cells.
    Type: Grant
    Filed: December 15, 2011
    Date of Patent: May 9, 2017
    Assignee: Cellulis, S.L.
    Inventors: Roberto Hernan Izquierdo, Natalia Gallot Escobal, Antonio Cruz Pacheco
  • Patent number: 9636292
    Abstract: Topical skin care composition for night use containing a combination of stabilized anti-oxidants, oat derived avenanthramides, retinol-like compounds and biofunctional peptides and other optional ingredients for topical application for the treatment and prevention of skin damage due to environmental factors.
    Type: Grant
    Filed: July 30, 2015
    Date of Patent: May 2, 2017
    Assignee: Truth Aesthetics LLC
    Inventors: Sara Sweeney, Fred H. Khoury