Abstract: Provided are dietary supplements, formulations, kits and methods for administration to an individual with type II diabetes or at risk of developing type II diabetes. Provided herein are formulations, kits and methods useful for treating, preventing, supporting, controlling, restoring, and/or maintaining blood sugar levels in individuals with type II diabetes or at risk of developing the same. Also provided are formulations, kits and methods useful for reducing and/or eliminating requirement of ex-vivo insulin administration in an individual diagnosed with type II diabetes.

Abstract: Methods of inducing or increasing lipid and protein production in microalgae are disclosed. Methods of enhancing microalgae biomass are also disclosed. The methods may comprise inoculating a culture media with microalgae and propagating the microalgae under heterotrophic growth conditions. The heterotrophic growth conditions may comprise inhibiting exposure of the inoculated culture media to light. The methods may further comprise delivering oxygen into the inoculated culture media. Additionally, lipids, proteins, and other cellular components may be isolated and purified from microalgae cultivated under the disclosed methods.

Abstract: Tissue rejuvenation methods include identifying a cell harvest site on a patient, identifying a cell injection site on the patient, harvesting tissue regenerative cells from the cell harvest site, agitating the tissue regenerative cells and injecting the tissue regenerative cells into the cell injection site. Tissue transfer devices suitable for implementation of the tissue rejuvenation methods are also disclosed.

Abstract: The invention relates to the preparation of carboxylic acid esters compounds, biodiesel (B) from microalgae, for use as a biofuel for compression type ignition. The objective is achieved through a hydroesterification process of a microalgae biomass (MU) comprising a hydrolysis stage and the esterification stage by means of heterogeneous catalysis. The wet microalgae biomass (MU) is the result of a process of cultivation concentration and is hydrolyzed directly in a hydrolysis column (20). Fatty acids (C), water (A) and raw glycerin (G) are obtained following the hydrolysis. The fatty acids (C) are esterified by reactive distillation in the presence of an impregnated heterogeneous catalyst (I).

Abstract: A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films.

Abstract: The method of growing the basidiomycete mycelium includes inoculating a substrate that promotes the growth and differentiation of basidiomycete mycelium without supporting the production of a basidiocarp with a vegetative mycelium and thereafter incubating the inoculated substrate in a first incubation period at controlled temperature, humidity, light and carbon dioxide levels followed by a finishing incubation period.

Abstract: A method is provided for the discrimination of cells from other particles, as well as of different types of cells in raw milk samples by impedance microflow cytometry. A method is provided of analysing the quality of raw milk in terms of its bacterial and somatic cell content without the need to pre-treat the milk sample, such that the analysis can take place directly at the production site. One advantage is discriminating and counting somatic cells from milk particles (consisting mainly of lipid vesicles) by high-frequency impedance analysis directly performed on untreated raw milk. Another advantage is that the method allows diagnosing the status of a mastitis infection directly after milking according to the analysis of the somatic cell count. Another advantage is to allow a fast method for determining the bacterial cell count in raw milk directly after milking. In addition, viability of both somatic and bacterial cells can be determined without the need of any cell label.

Abstract: A process for increasing a production of alcohol by fermenting sugars may include applying a direct current electrical field to a fermentation broth, which occurs before the fermentation broth inoculates with a concentration of yeasts; and controlling a pH value of said fermentation broth to maintain the pH value with a specific range.

Abstract: A pseudo-esterase activity-based fluorescent probe for specific detection of albumin, which has a carboxylic ester bond that can be selectively cleaved by human serum albumin (HSA), therefore forming a hydrolysate, which has a fluorescence emission spectrum significantly different from that of the fluorescent probe. According to the fluorescence intensity of the fluorescent probe and hydrolysate, we can detect the content of HSA in a biological sample.

Abstract: The Lactobacillus plantarum CCFM8661 is tolerant to acid and lead ions in vitro which can tolerate lead ions solution with the initial concentration of 150 mg/L, and has a strong capability of binding lead ions, which can reduce the lead level in mice blood, liver, kidney and stomach, significantly improve antioxidant indicators and alleviate pathological symptoms of lead exposed mice.

Abstract: A microbial consortium comprises of an Aureobasidium sp. to metabolise a sugar substrate into fructooligosaccaride, glucose and fructose and a Pachysolen sp to metabolise the glucose and the fructose into ethanol.

Abstract: An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.

Abstract: Oleaginous yeast strains are used to hydrolyze biomass (e.g. wheat straw) that has been pretreated using dilute acid, in order to produce lipids. The lipids may be used as feedstock for producing biofuels.

Abstract: The teachings provided herein generally relate to site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites. More particularly, the systems taught here include a phenolic compound bound to a reactive oxygen species, wherein the phenolic compound and the reactive oxygen species react at a target area in the presence of an oxidoreductase enzyme.

Abstract: Provided is an agent or a food or drink for reducing the risk of developing breast cancer. An agent for reducing the risk of developing breast cancer which comprises a composition containing living cells of Lactobacillus casei.

Abstract: Human umbilical cord lining stem cells that are capable of differentiating into cells of the mesodermal lineage and ectodermal lineage are described, as well as methods of isolating, expanding, culturing, and cryopreserving such cells.

Abstract: The present invention features a novel protein engineering strategy by combining the domains of two independent proteins into a molecular switch. The invention features polypeptides comprising a prodrug activating enzyme and a protein that binds a cancer specific marker, polynucleotides encoding the polypeptides, and molecular switches for converting a prodrug into a toxin, comprising the polypeptides. The invention also features methods for converting a prodrug into a toxin, methods for treating cancer, and methods for making the molecular switches, as well as kits.

Abstract: This invention provides a means for modifying surface properties of a cell culture substrate under specific conditions, to thereby regulate regions to which cells are allowed to adhere or are not allowed to adhere, depending on cell type. This invention relates to a method of cell culture comprising steps of: applying a positive potential to a conductive region of a substrate comprising a base material having a conductive region and a non-cell-adhesive membrane coupled thereto with the aid of silane, so as to separate the non-cell-adhesive membrane from the substrate; and culturing cells in a region from which the non-cell-adhesive membrane has been separated.

Abstract: Treatment methods/devices are provided for attenuating/inactivating the pathogenic microbes found in biological fluids e.g. blood/blood products including human single-donor-fresh-frozen-plasma, platelet concentrate, red blood cells, blood clotting factors. An Antimicrobial Photodynamic Therapy method is used to eliminate multiple (resistant) bacteria, viral agents, fungi, parasites and other undetected or non-easily detected pathogenic microbes or particles in blood and blood products without affecting their biological properties. Resistant bacteria are difficult to be eliminated. This is especially true in the case for S. aureus and related strains, Staphylococcus epidermidis or Propionibacterium acnes, Borrelia species and other bacteria found on skin. Further embodiments eliminate undetected or non-easily detected viral agents contaminating blood/blood products responsible for spreading hepatitis, Acquired ImmunoDeficiency Syndrome and other blood borne viral diseases.

Abstract: The teachings provided herein generally relate to site-activated binding systems that selectively increase the bioactivity of phenolic compounds at target sites. More particularly, the systems taught here include a phenolic compound bound to a reactive oxygen species, wherein the phenolic compound and the reactive oxygen species react at a target area in the presence of an oxidoreductase enzyme.