Patents Examined by T. D. Wessendorf
  • Patent number: 8492530
    Abstract: The present invention provides unstructured recombinant polymers (URPs) and proteins containing one or more of the URPs. The present invention also provides microproteins, toxins and other related proteinaceous entities, as well as genetic packages displaying these entities. The present invention also provides recombinant polypeptides including vectors encoding the subject proteinaceous entities, as well as host cells comprising the vectors. The subject compositions have a variety of utilities including a range of pharmaceutical applications.
    Type: Grant
    Filed: November 3, 2010
    Date of Patent: July 23, 2013
    Assignee: Amunix Operating Inc.
    Inventors: Volker Schellenberger, Willem P. Stemmer, Chia-Wei Wang, Michael D. Scholle, Mikhail Popkov, Nathaniel C. Gordon, Andreas Crameri
  • Patent number: 8470745
    Abstract: Methods for isolating and using multi-protein complexes that are biologically active are provided. The complexes contain one or more proteins of interest (e.g. a receptor, ion channel, etc.) and associates scaffolding proteins such as phosphatases, kinases and post synaptic density components. Buffers that do not contain denaturing agents and which may be used to isolate the multi-protein complexes are also provided, as are protein arrays containing the biologically active multi-protein complexes. The protein arrays may be used, for example, for high throughput screening assays.
    Type: Grant
    Filed: February 10, 2012
    Date of Patent: June 25, 2013
    Inventor: Karima Ferrani-Kile
  • Patent number: 8466090
    Abstract: A method for high throughput screening of probes is described. These probes are useful for characterization and measurement of unbound metabolites in a fluid sample, particularly characterization and measurement of levels of unbound free fatty acids. By practice of the disclosed invention, a profile of unbound metabolites can be determined for an individual which can be used to determine the individual's relative risk for disease such as stroke, cardiac disease and cancer.
    Type: Grant
    Filed: August 4, 2009
    Date of Patent: June 18, 2013
    Assignee: FFA Sciences LLC
    Inventors: Alan Marc Kleinfeld, Andrew Henry Huber, James Patrick Kampf, Thomas Kwan, Baolong Zhu
  • Patent number: 8372954
    Abstract: Phage display libraries are taught in which the recombinant phage population displays a plurality of potential binding fragments having preferred characteristics of solubility and/or intermolecular interaction. Also taught are methods of biasing display libraries to produce variants which more closely approximate the preferred characteristics of the parental binding fragment.
    Type: Grant
    Filed: December 21, 2001
    Date of Patent: February 12, 2013
    Assignee: National Research Council of Canada
    Inventors: Jamshid Tanha, Joycelyn Entwistle, Saran Narang, Michael Dan, Colin R. Mackenzie, Carole Grad
  • Patent number: 8357637
    Abstract: The present invention is based, at least in part, on the identification of molecules involved in the differentiation and/or activity of osteoblasts and osteoclasts. Accordingly, the present invention provides methods of identifying modulators of bone formation, mineralization, and/or osteoclastogenesis and methods for treating disorders that would benefit from modulation of bone formation, mineralization, and/or osteoclastogenesis using agents identified as described herein.
    Type: Grant
    Filed: May 29, 2008
    Date of Patent: January 22, 2013
    Assignee: Cornell University
    Inventors: Laurie H. Glimcher, Dallas C. Jones, Antonios O. Aliprantis
  • Patent number: 8338124
    Abstract: The present invention relates to in vivo and in vitro methods, agents and compound screening assays for inducing differentiation of undifferentiated mammalian cells into osteoblasts, including bone formation enhancing pharmaceutical compositions, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean bone density in a subject.
    Type: Grant
    Filed: November 21, 2009
    Date of Patent: December 25, 2012
    Assignee: Galapagos N.V.
    Inventors: Luc Juliaan Corina Van Rompaey, Peter Herwig Maria Tomme, Robin John Brown
  • Patent number: 8334238
    Abstract: The invention relates to novel methods of detecting alterations in cell cycle regulation in a cell or a cell population and screening for agents capable of modulating cell cycle regulation through the use of multiparameter assays and a fluorescence-activated cell sorter (FACS) machine.
    Type: Grant
    Filed: April 16, 1999
    Date of Patent: December 18, 2012
    Assignee: Rigel, Inc.
    Inventors: Joseph Fisher, James Lorens, Donald Payan, Alexander Rossi
  • Patent number: 8318721
    Abstract: A method and composition are provided for the treatment of an anorectal disorder and for controlling the pain associated therewith. The method comprises administering to a subject in need of such treatment therapeutically effective amounts of a calcium channel blocker either alone or together with a nitric oxide donor. Amlodipine, anipamil, barnidipine, benidipine, bepridil, darodipine, diltiazem, efonidipine, felodipine, isradipine, lacidipine, lercanidipine, lidoflazine, manidipine, mepirodipine, nicardipine, nifedipine, niludipine, nilvadipine, nimodipine, nisoldipine, nitrendipine, perhexiline, tiapamil, verapamil and pharmaceutically acceptable salts thereof, are suitable calcium channel blockers.
    Type: Grant
    Filed: July 8, 2011
    Date of Patent: November 27, 2012
    Assignee: S.L.A. Pharma AG
    Inventors: Michael A. Kamm, Robin K. S. Phillips
  • Patent number: 8304229
    Abstract: A process for preparing a substrate having thereon sites reactive with the reactive groups of a molecular probe, the process comprising the steps of applying to the substrate surface a material comprising one or more reactive sites having an activated ethylenically unsaturated double bond of the formula I: wherein the substituents are defined in the specification and, in a further step, covalently binding the reactive group of the molecular probe to the activated ethylenically unsaturated double bond of the reactive sites having formula I.
    Type: Grant
    Filed: March 20, 2002
    Date of Patent: November 6, 2012
    Assignee: Cytec Surface Specialties, S.A.
    Inventors: Luc Lindekens, Michel Tielemans, Steven Cappelle, Jose Remacle, Benoit De Becker
  • Patent number: 8287700
    Abstract: The present invention describes extremely rapid and efficient methods for the attachment of chemical moieties to matrices by the use of microwave technology. The methods of the invention can be applied in a variety of ways for the preparation of different types of matrices for a variety of applications including but not limited to the functionalization of various solid supports, and matrices in the form of powder, beads, sheets, and other suitable surfaces for use in applications including but not limited to oligonucleotide synthesis, peptide synthesis, environmental clean up (removal of toxic materials), immunoassays, affinity chromatography, combinatorial chemistry, microarrays, proteomics and medical diagnostics.
    Type: Grant
    Filed: December 17, 2009
    Date of Patent: October 16, 2012
    Assignee: Spring Bank
    Inventors: Seetharamaiyer Padmanabhan, Radhakrishnan P. Iyer
  • Patent number: 8268756
    Abstract: Described are methods for producing libraries of cells expressing at least two separate single polypeptide chain binding proteins, in which the binding proteins have different target epitopes. Such libraries are made by integration of the nucleic acid sequences encoding the polypeptide chains into the genome of the host cell, and selecting for cells that have successfully integrated these nucleic acids. The selected cells are preferably subjected to a cloning step. Mixtures of binding proteins are produced without having to individually produce each of the components of the mixture. A library of cells wherein essentially each cell encodes at least two single polypeptide chain binding proteins having different target epitopes is also herewith provided, as well as methods for producing a composition comprising at least two separate single polypeptide chain binding proteins having different target epitopes.
    Type: Grant
    Filed: July 20, 2006
    Date of Patent: September 18, 2012
    Assignee: Merus B.V.
    Inventors: Ton Logtenberg, Hendricus Renerus Jacobus Mattheus Hoogenboom
  • Patent number: 8263741
    Abstract: The present invention provides a fibronectin type III (Fn3) molecule, wherein the Fn3 contains a stabilizing mutation. The present invention also provides Fn3 polypeptide monobodies, nucleic acid molecules encoding monobodies, and variegated nucleic acid libraries encoding such monobodies. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform the methods.
    Type: Grant
    Filed: July 11, 2001
    Date of Patent: September 11, 2012
    Assignee: Research Corporation Technologies, Inc.
    Inventor: Shohei Koide
  • Patent number: 8207093
    Abstract: Described herein are RNA-protein fusion production methods which involve a high salt post-translational incubation step.
    Type: Grant
    Filed: July 6, 2007
    Date of Patent: June 26, 2012
    Assignee: The General Hospital Corporation
    Inventors: Jack W. Szostak, Richard W. Roberts, Rihe Liu
  • Patent number: 8158158
    Abstract: A biocidal composition is disclosed comprising composite particles, each of said composite particles containing a shell and a core, said core comprising a metal or a metal-containing compound wherein the metal is a moiety selected from the group consisting of zinc, copper, bismuth, silver, zirconium, and combinations thereof said shell comprising a pyrithione adduct comprising the reaction product of pyrithione with a portion of said core metal or metal compound. In one aspect, an anti-fouling composition is disclosed comprising (a) an anti-soft-fouling effective amount of copper pyrithione; and (b) an anti-hard-fouling effective amount of a copper-containing salt, or oxide or hydroxide thereof. The present invention also relates to a method of making an anti-fouling composition comprising particles of copper pyrithione and a copper-containing salt that is effective against hard-fouling and soft-fouling organisms.
    Type: Grant
    Filed: February 15, 2002
    Date of Patent: April 17, 2012
    Assignee: Arch Chemicals, Inc.
    Inventors: David F. Gavin, Craig Waldron, Robert J. Martin, George A. Polson
  • Patent number: 8119572
    Abstract: A method for determining protein binding specificity using a screen of a peptide library is provided. The method can be used to determine binding specificity for human NAD+-dependent deacetylase SIRT1, and to identify the most efficiently deacetylated peptide sequences. The method can be also used to screen a combinatorial H4 histone N-terminal tail peptide library to examine the binding preferences of a ?-phos (S1) H4 antibody toward all known possible H4 histone modification states.
    Type: Grant
    Filed: October 24, 2006
    Date of Patent: February 21, 2012
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: John M. Denu, Adam L. Garske
  • Patent number: 8105983
    Abstract: An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.
    Type: Grant
    Filed: March 29, 2006
    Date of Patent: January 31, 2012
    Assignee: The Regents of the University of California
    Inventors: Sung-Hou Kim, Rosalind Kim, Jamila Jancarik
  • Patent number: 8088892
    Abstract: A method for screening peptides with an aptamer library for determining inhibitors of any one of the proteins from the Rho-GEFs family.
    Type: Grant
    Filed: September 15, 2008
    Date of Patent: January 3, 2012
    Assignee: Centre National de la Recherche Scientifique (C.N.R.S.)
    Inventors: Anne Debant, Susanne Schmidt
  • Patent number: 8048875
    Abstract: Bethanechol, diltiazem, or a combination thereof is administered locally to the anus for the treatment of benign anal disorders, in particular anal fissures and hemorrhoids. The agents induce a reduction in the mean and resting pressure, thereby assisting in the healing of the anal fissures and hemorrhoids.
    Type: Grant
    Filed: February 23, 1997
    Date of Patent: November 1, 2011
    Assignee: S.L.A. Pharma AG
    Inventors: Michael A. Kamm, Robin K. S. Phillips
  • Patent number: 8017559
    Abstract: The present invention provides novel polypeptides having the scaffold structure of a C-type lectin-like domain (CTLD) and a randomized loop region for specifically binding a variety of target compounds and also provides nucleic acids encoding the polypeptides. The present invention further provides combinatorial CTLD libraries, methods for constructing the libraries, and methods for screening the libraries to identify and isolate the novel CTLD polypeptides. Specifically, the invention provides libraries of nucleic acids encoding polypeptides having a scaffold CTLD with a randomized loop region, as well as nucleic acid sequences, vectors, and methods for preparing and expressing the libraries. Exemplary nucleic acids useful in the combinatorial libraries are derived from tetranectin and other proteins having a CTLD.
    Type: Grant
    Filed: December 4, 2006
    Date of Patent: September 13, 2011
    Assignee: Anaphore, Inc.
    Inventors: Michael Etzerodt, Thor Las Holtet, Niels Jonas Heilskov Graversen, Hans Christian Th√łgersen
  • Patent number: RE43134
    Abstract: The present invention provides a denatured albumin lamina, useful for repairing lesions on solid visceral organs. The lamina comprises human serum albumin, formed into a thin, pliant sheet and denatured. The denatured lamina can be sterilized and stored until used. As well, it can be impregnated with a variety of bioagents. A method for repairing a lesion on a solid visceral organ includes applying an energy-absorbing proteinaceous material to a lesion site on the solid visceral organ lesion; irradiating the proteinaceous material with energy sufficient to fuse the energy-absorbing material at least partially to the lesion site; applying a biocompatible denatured albumin lamina onto the proteinaceous material on the lesion site; and irradiating the biocompatible albumin lamina and the proteinaceous material with energy sufficient to fuse the biocompatible albumin lamina to the proteinaceous material and/or the lesion site.
    Type: Grant
    Filed: April 8, 2005
    Date of Patent: January 24, 2012
    Assignee: Providence Health System - Oregon
    Inventors: Yasmin Wadia, Scott Alan Prahl