Patents Examined by T. D. Wessendorf
  • Patent number: 7994096
    Abstract: The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with a synthetic peptide library, by preparing a plurality of peptides, each of said peptides comprising a fragment less than the whole of the total amino acid sequence of the antigen, each of said peptides being a minimum of 9 amino acid residues (AAs) in length, and the amino acid sequence of each peptide overlapping with the amino acid sequence of at least one other of said peptides; combining a plurality of the peptides from a) into a peptide library, said peptide library comprises a plurality of the peptides selected such that their collective overlapping amino acid sequences span the total amino acid sequence of the antigen; and incubating a suspension comprising CD8+ T lymphocytes, CD4+ T lymphocytes or a mixture of CD8+ and CD4+ with said peptide library in a single culture run.
    Type: Grant
    Filed: February 17, 2001
    Date of Patent: August 9, 2011
    Inventors: Florian Kern, Hans-Dieter Volk, Petra Reinke, Nicole Faulhaber, Ingolf-Pascal Surel, Elham Khatamzas
  • Patent number: 7981842
    Abstract: A method is provided for identifying complexes between a transcription factor and another protein, such as another different transcription factor. The method includes the steps of isolating from a biological sample transcription factor complexes based on whether the transcription factor complexes include a particular type of transcription factor; and identifying which of the multiple different proteins are present in the isolated transcription factor complexes.
    Type: Grant
    Filed: September 5, 2001
    Date of Patent: July 19, 2011
    Assignee: Panomics, Inc.
    Inventor: Xianqiang Li
  • Patent number: 7981843
    Abstract: The present invention provides methods of making antibodies having the binding specificity of a reference antibody. Antibodies generated by the methods of the inventions have at least one minimal essential binding specificity determinant from a heavy chain or light chain CDR3 from the reference antibody. The method can be used, e.g., in humanization procedures. The invention also provides libraries and antibodies made in accordance with the methods.
    Type: Grant
    Filed: January 20, 2005
    Date of Patent: July 19, 2011
    Assignee: KaloBios Pharmaceuticals, Inc.
    Inventors: Peter Flynn, Kenneth Luehrsen, Robert F. Balint, Jeng-Horng Her, Christopher R. Bebbington, Geoffrey T. Yarranton
  • Patent number: 7981601
    Abstract: The invention relates to a method for preparing polyepitope chimeric gene vaccines, designated as Epitopes Assemble Library Immunization (EALI). It involves the construction of expression libraries of polyepitope chimeric genes with different sizes and lengths using gene shuffling and random assembly so as to screen polyepitope chimeric gene vaccines. Immunization of the body with the gene libraries of the invention results in the induction of high level of specific antibodies and specific types of cytokines, and protection on the body.
    Type: Grant
    Filed: August 1, 2003
    Date of Patent: July 19, 2011
    Assignees: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
    Inventors: Heng Wang, Qiliang Cai
  • Patent number: 7943174
    Abstract: A solid oral controlled-release oral dosage form of hydrocodone is disclosed. The dosage form comprising an analgesically effective amount of hydrocodone or a pharmaceutically acceptable salt thereof, and a sufficient amount of a controlled release material to render the dosage form suitable for twice-a-day administration to a human patient, the dosage form providing a C12/Cmax ratio of 0.55 to 0.85, said dosage form providing a therapeutic effect for at least about 12 hours.
    Type: Grant
    Filed: June 9, 2004
    Date of Patent: May 17, 2011
    Assignee: Purdue Pharma L.P.
    Inventors: Benjamin Oshlack, Hua-Pin Huang, John Masselink, Alfred P. Tonelli
  • Patent number: 7915221
    Abstract: The present invention provides a method for the design and/or the selection of agonist or antagonist chemokine variants combining a phage display technology and a screening on living cells expressing the receptor of the corresponding native chemokine. It also provides RANTES variants having agonist properties towards said receptor, and methods for preventing and/or curing viral diseases, as well as clues for preventing and/or curing inflammatory or malignant diseases.
    Type: Grant
    Filed: April 2, 2007
    Date of Patent: March 29, 2011
    Assignee: Universite Pierre et Marie Curie (Paris VI)
    Inventors: Guy Gorochov, Oliver Hartley, Karim Dorgham, Patrice Debre, Robin Offord
  • Patent number: 7902121
    Abstract: T cells are profiled with respect to their expression of antigen receptor. The cells are arrayed on a planar or three-dimensional substrate through binding to immobilized or partially diffused MHC-antigen complexes. The cells may further be characterized with respect to their ability to respond to external stimulus in the microenvironment. External stimuli include cell-cell interactions, response to factors, and the like.
    Type: Grant
    Filed: May 27, 2004
    Date of Patent: March 8, 2011
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Daniel Shin-Yu Chen, Yoav Soen, Daniel Lewis Kraft, Patrick O. Brown, Mark Davis
  • Patent number: 7879545
    Abstract: A classifier to predict cellular radiation sensitivity based on gene expression profiles in thirty-five cell lines from the NCI panel of 60 cancer cell lines (NCI-60), using a novel approach to predictive gene analysis. Three novel genes are provided, retinoblastoma binding protein 4 (RbAp48), G-protein signaling regulator 19 (RGS19) and ribose-5-phosphate isomerase A (R5PIA) whose expression values were correlated with radiation sensitivity.
    Type: Grant
    Filed: November 4, 2004
    Date of Patent: February 1, 2011
    Assignees: H. Lee Moffitt Cancer Center and Research Institute, Inc., University of South Florida
    Inventors: Javier F. Torres-Roca, Timothy Yeatman, Steven Eschrich
  • Patent number: 7825070
    Abstract: A novel method for two-dimensionally arraying ferritin on a substrate is provided which obviates the need for a metal ion that permits linking between adjacent two ferritin particles. In a method of two-dimensionally arraying ferritin on a substrate, the surface of the substrate is hydrophilic, and the method includes the steps of: developing a solution containing a solvent and the ferritin on the substrate; and removing the solvent from the solution developed on the substrate, while the ferritin has an amino acid sequence set out in SEQ ID NO: 1 modified at its N-terminus.
    Type: Grant
    Filed: November 13, 2008
    Date of Patent: November 2, 2010
    Assignee: Panasonic Corporation
    Inventors: Takuro Matsui, Nozomu Matsukawa, Kazuaki Nishio
  • Patent number: 7811994
    Abstract: A method for the identification of peptides having an affinity for the surface of fungi is provided as is a method for the identification of peptides capable of affecting the development of a fungus. Also provided are compositions containing peptides identified using the method of the present invention. In addition, isolated polynucleotides, vectors, expression cassettes and transformed cells capable of expressing peptides identified by the method of the present invention are provided.
    Type: Grant
    Filed: April 13, 2009
    Date of Patent: October 12, 2010
    Assignee: The Curators of the University of Missouri
    Inventors: James T. English, Francis J. Schmidt, George P. Smith, Roy O. Morris, Sharon Bishop-Hurley
  • Patent number: 7803765
    Abstract: The present invention provides novel methods for producing nucleic acid fragment libraries that express highly diverse peptides or protein domains and, in particular, methods for producing nucleic acid fragment libraries wherein the nucleic acid.
    Type: Grant
    Filed: February 20, 2004
    Date of Patent: September 28, 2010
    Assignee: Phylogica Limited
    Inventors: Paul Michael Watt, Wayne Robert Thomas, Richard Hopkins
  • Patent number: 7799901
    Abstract: The present invention provides proteins capable of modulating or mediating the FAS receptor ligand or TNF effect on cells carrying FAS receptor or p55 receptor by binding or interacting with MORT-1 protein, which in turn binds to the intracellular domain of the FAS receptor or to another protein TRADD which binds to the p55 receptor. In addition, peptide inhibitors that interfere with the proteolytic activity of MORT-1-binding proteins having proteolytic activity are provided, as well as a method of designing them.
    Type: Grant
    Filed: September 18, 2006
    Date of Patent: September 21, 2010
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: David Wallach, Mark Boldin, Tanya Goncharov, Yury V. Golstev
  • Patent number: 7794962
    Abstract: The invention provides a method for the expression of exogenous DNA libraries in filamentous fungi. The fungi are capable of processing intron-containing eukaryotic genes, and also can carry out post-translational processing steps such as glyclosylation and protein folding. The invention provides for the use of fungi with altered morphology, which permits high-throughput screening and directed molecular evolution of expressed proteins. The same transformed fungi may be used to produce larger quantities of protein for isolation, characterization, and application testing, and may be suitable for commercial production of the protein as well.
    Type: Grant
    Filed: July 21, 2006
    Date of Patent: September 14, 2010
    Assignee: Dyadic International (USA), Inc.
    Inventors: Mark A. Emalfarb, Peter J. Punt, Cornelia van Zeijl, Cornelius van den Hondel
  • Patent number: 7781173
    Abstract: The invention provides populations of molecules that are prepared as, or treated to become, homogeneous for one or more molecular characteristics. In an aspect, the invention relates to molecular weight standards that may be used to determine the molecular weight or apparent molecular weight of uncharacterized molecules, such as proteins and nucleic acids, as well as in other applications. In one aspect, the molecular weight standards are pre-stained.
    Type: Grant
    Filed: September 24, 2004
    Date of Patent: August 24, 2010
    Assignee: Life Technologies Corporation
    Inventors: Joseph W. Amshey, Roumen A. Bogoev
  • Patent number: 7777010
    Abstract: The invention provides a method for selecting at least one member from a library of proteinaceous molecules by providing at least one cell and/or a functional equivalent thereof, with at least part of the library under conditions that allow binding of any such member to an epitope in and/or on the cells and/or the functional equivalent thereof, removing unbound proteinaceous molecules and selecting the at least one member, wherein the library includes at least one mutant of a proteinaceous molecule capable of binding to the epitope.
    Type: Grant
    Filed: June 28, 2002
    Date of Patent: August 17, 2010
    Assignee: Crucell Holland B.V.
    Inventor: Ton Logtenberg
  • Patent number: 7718575
    Abstract: A method of selecting a test molecule that binds to HLA-DP4 by (i) incubating HLA-DP4 with the test molecule and a labeled peptide of formula (I) Z1X1X2X3X4X5X6X7X8X9Z2 thereby forming respective complexes, wherein Z1 and Z2, which may be identical or different, are each either zero or from 1 to 100 amino acids; X1 is an aromatic or hydrophobic amino acid, or S; X6 is an aromatic or hydrophobic amino acid, or C; X9 is an aromatic or hydrophobic amino acid, or C, D, Q, S, T, or E; and X2, X3, X4, X5, X7and X8 are each an amino acid; (ii) separating the respective complexes formed; (iii) detecting the HLA-DP4/labeled peptide complexes; and (iv) selecting the test molecule that exhibits a binding activity of IC50<1000 nM, which corresponds to the concentration of the test molecule that inhibits 50% of the competitive HLA-DP4 binding of the labeled peptide.
    Type: Grant
    Filed: October 17, 2002
    Date of Patent: May 18, 2010
    Assignees: Commissariat a l'Energie Atomique, Sedac Therapeutics
    Inventors: Bernard Maillere, Florence Castelli, C├ęcile Buhot, Bertrand Georges
  • Patent number: 7700523
    Abstract: The invention relates to a zinc finger polypeptide library in which each polypeptide comprises more than one zinc finger which has been at least partially randomized, and to a set of zinc finger polypeptide libraries which encode overlapping zinc finger polypeptides, each polypeptide comprising more than one zinc finger which has been at least partially randomized, and which polypeptide may be assembled after selection to form a multifinger zinc finger polypeptide.
    Type: Grant
    Filed: September 1, 2006
    Date of Patent: April 20, 2010
    Assignee: Gendaq Limited
    Inventors: Yen Choo, Aaron Klug, Mark Isalan
  • Patent number: 7696326
    Abstract: The invention of the present application relates to a method for obtaining antibodies directed against a carbohydrate moiety contained in a carbohydrate peptide conjugate, by administering to a subject a composition containing a carbohydrate peptide conjugate and collecting the antibodies directed against the said carbohydrate moiety from a serum previously obtained from the subject.
    Type: Grant
    Filed: January 3, 2007
    Date of Patent: April 13, 2010
    Assignee: Institut Pasteur
    Inventors: Sylvie Bay, Daniele Cantacuzene, Claude Leclerc, Richard Lo-Man
  • Patent number: 7666817
    Abstract: The present invention provides compositions including peptide display scaffolds that present at least one candidate peptide and at least one detectable moiety in at least one of the N-terminal and C-terminal candidate peptide presenting domains that when expressed in a cell are accessible at a surface of the cell outermembrane. In addition, the present invention also provides kits and methods for screening a library of cells presenting the candidate peptides in peptide display scaffolds to identify a ligand for an enzyme.
    Type: Grant
    Filed: August 30, 2006
    Date of Patent: February 23, 2010
    Assignee: The Regents of the University of California
    Inventors: Patrick Sean Daugherty, Kevin T. Boulware
  • Patent number: 7638288
    Abstract: The present invention relates to in vivo and in vitro methods, agents and compound screening assays for inducing differentiation of undifferentiated mammalian cells into osteoblasts, including bone formation enhancing pharmaceutical compositions, and the use thereof in treating and/or preventing a disease involving a systemic or local decrease in mean bone density in a subject.
    Type: Grant
    Filed: June 29, 2006
    Date of Patent: December 29, 2009
    Assignee: Galapagos N.V.
    Inventors: Luc Juliaan Corina Van Rompaey, Peter Herwig Maria Tomme, Robin John Brown