Patents Examined by Taeyoon Kim
  • Sample processing method and sample culturing method
    Patent number: 11124820
    Abstract: Provided is a sample processing method that liquefies a medium solution by making a liquid that liquefies the medium solution act on a sample formed by gelating or solidifying the medium solution that is supported by a substrate while an observation subject is included therein, while maintaining a state in which the medium solution is supported by the substrate while the observation subject is included therein.
    Type: Grant
    Filed: December 17, 2018
    Date of Patent: September 21, 2021
    Assignee: OLYMPUS CORPORATION
    Inventor: Katsunori Ogo
  • Method for treating multiple sclerosis
    Patent number: 11110129
    Abstract: A method for treating a multiple sclerosis is provided. The method includes administering a composition containing a plurality of mesenchymal stem cells to a subject in need for a treatment of the multiple sclerosis, in which each of the mesenchymal stem cells expresses insulin-like growth factor 1 receptors.
    Type: Grant
    Filed: March 14, 2019
    Date of Patent: September 7, 2021
    Assignee: China Medical University
    Inventors: Woei-Cherng Shyu, Chen-Huan Lin, Wei Lee, Chia-Hung Hsieh, Chung-Y Hsu, Chang-Hai Tsai
  • Neural cell extracellular vesicles
    Patent number: 11111475
    Abstract: Disclosed herein are neural extracellular vesicles (EVs) and methods of using these EVs in the treatment of spinal cord injury, stroke, and traumatic brain injury and neurodegenerative diseases.
    Type: Grant
    Filed: May 16, 2019
    Date of Patent: September 7, 2021
    Assignees: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC., ARUNA BIO, INC.
    Inventors: Steven L. Stice, Robin Lynn Webb, Tracy A. Stice
  • Live cell constructs for production of cultured milk product and methods using the same
    Patent number: 11111477
    Abstract: This invention relates to live cell constructs for in vitro and/or ex vivo production of cultured milk products from mammary cells, methods of producing isolated cultured milk products from mammary cells, bioreactors for producing isolated cultured milk products, and cultured milk products.
    Type: Grant
    Filed: December 18, 2020
    Date of Patent: September 7, 2021
    Assignee: BIOMILQ, Inc.
    Inventor: Leila Strickland
  • Method for manufacturing ciliary marginal zone-like structure
    Patent number: 11111476
    Abstract: The present invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure, including a step of culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more and 100% or less of the tissue in a serum-free medium or serum-containing medium each containing a substance acting on the Wnt signal pathway and a substance inhibiting the FGF signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene-expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium each free of a substance acting on the Wnt signal pathway and so on. According to the production method of the present invention, a ciliary marginal zone-like structure can be produced with high efficiency.
    Type: Grant
    Filed: February 5, 2019
    Date of Patent: September 7, 2021
    Assignees: Sumitomo Chemical Company, Limited, RIKEN
    Inventors: Atsushi Kuwahara, Yoshiki Sasai
  • Serum-free culture medium and preparation method and application therefor
    Patent number: 11098280
    Abstract: Provided is a serum-free culture medium, the ingredients of the culture medium comprising 0.05-0.2 parts by volume of ?-mercaptoethanol, 0.5-2 parts by volume of non-essential amino acid aqueous solution, 4-6 parts by volume of human mesenchymal stem cell culture supernatant concentrate, and 90-95 parts by volume of a-MEM/DMEM-F12 and recombinant human alkaline fibroblast growth factor of a final concentration of 5-5 ng/ml. The present culture medium is used for carrying out stem cell culture.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: August 24, 2021
    Inventors: Lei Guo, Cheng Li
  • Formulation of fat-soluble vitamin
    Patent number: 11090276
    Abstract: The invention relates to a composition comprising A) microcapsules comprising at least one fat-soluble active substance selected from a vitamin K compound or a provitamin or a prodrug of a vitamin K compound embedded in a matrix comprising a hydrocolloid and optionally one or more other matrix components, and B) at least one dietary mineral; as well as uses and products comprising such compositions.
    Type: Grant
    Filed: May 5, 2015
    Date of Patent: August 17, 2021
    Assignee: BASF SE
    Inventors: Inger Reidun Aukrust, Helena M. Larsson, Thomas Rove
  • Reagent kit for step-by-step hUC-MSC culture and hUC-MSC acquired using said reagent kit
    Patent number: 11091739
    Abstract: A serum-free culture method for human umbilical cord mesenchymal stem cells (hUC-MSC), said method using a step-by-step method to culture hUC-MSC: first using a TME culture medium for culturing for 3-4 hours to promote hUC-MSC adherence, and then switching to a TMD culture medium for rapid amplification.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: August 17, 2021
    Inventors: Lei Guo, Cheng Li
  • Factors and cells that provide for induction of bone, bone marrow, and cartilage
    Patent number: 11083755
    Abstract: Methods, compositions and kits for producing functional chondrocytes, skeletal cells, bone marrow stromal cells, and progenitor cells thereof are provided. These methods, compositions and kits find use in producing chondrocytes, osteoblasts, stromal cells, and progenitor cells thereof in vivo, or in vitro for transplantation, for experimental evaluation, as a source of lineage- and cell-specific products, and the like, for example for use in treating human disorders of the cartilage, bone and hematopoietic system. In some embodiments, specific combinations of protein factors are identified for reprogramming non-skeletal cells into bones, hematopoietic stroma, and chondrocytes, which may be provided in vitro or in vivo.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: August 10, 2021
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Charles K. F. Chan, Irving L. Weissman, Michael T. Longaker
  • Glycosylated oxalate decarboxylase and preparation and application thereof
    Patent number: 11085033
    Abstract: The present invention discloses a glycosylated oxalate decarboxylase. The oxalate decarboxylase is derived from edible basidiomycetes, the glycosylated oxalate decarboxylase has an enzyme activity at the pH value of 1.5-7.5, the activity at the pH value of 1.5-2.0 is 10% greater than the optimum activity, and a specific activity is more than 2 U/mg. The present invention also discloses preparation and application of the glycosylated oxalate decarboxylase. The glycosylated oxalate decarboxylase disclosed by the present invention can keep the activity under a low pH value, and can effectively prevent and treat kidney stones.
    Type: Grant
    Filed: July 7, 2017
    Date of Patent: August 10, 2021
    Assignee: WUHAN KANGFUDE BIOTECHNOLOGY CO., LTD.
    Inventors: Haifeng Liu, Baoping Song, Xianqiao Chen, Huoqing Chen, Pei Tong, Wei Wang, Xiaofeng Wang
  • Method for ex vivo expansion of regulatory T cells
    Patent number: 11072779
    Abstract: The invention relates to a new method for in vitro expansion of CD4+CD25HighCD127?/LOWfoxP3+Tregs, wherein the process of Treg expansion takes place permanently or temporarily at a temperature below 37° C., optimally at a temperature of 33° C., the isolated Tregs are expanded in SCGM or X-vivo-20 medium supplemented with human serum or with foetal bovine serum, and magnetic beads coated with anti-CD3 and anti-CD28 antibodies at 1:1 (cell:bead) ratio and interleukin-2 are added to the culture.
    Type: Grant
    Filed: December 19, 2016
    Date of Patent: July 27, 2021
    Assignee: GDANSKI UNIWERSYTET MEDYCZNY
    Inventors: Natalia Marek-Trzonkowska, Piotr Trzonkowski, Malgorzata Mysliwiec
  • Natural killer cells with enhanced viability, proliferation and cytotoxicity following cryopreservation
    Patent number: 11066643
    Abstract: The present disclosure generally relates to compositions of NK cells for adoptive transfer. In particular, the disclosure relates to enhancing viability, proliferation and cytotoxicity of feeder-free NK cells following cryopreservation.
    Type: Grant
    Filed: May 13, 2020
    Date of Patent: July 20, 2021
    Inventors: Nickolas Chelyapov, Rafael Gonzalez
  • Enhanced stem cell composition
    Patent number: 11052118
    Abstract: The invention provides improved methods for cell therapy. In particular, the invention provides therapeutic compositions of enhanced hematopoietic stem and progenitor cells having improved engraftment and homing properties, and methods of making the therapeutic compositions. The invention further provides methods of improving the efficacy of hematopoietic stem and progenitor cell transplantation including transplanting the therapeutic composition to subjects in need of hematopoietic system reconstitution.
    Type: Grant
    Filed: December 12, 2018
    Date of Patent: July 6, 2021
    Assignee: Fate Therapeutics, Inc.
    Inventors: Daniel Shoemaker, David Robbins, John D. Mendlein, Caroline Desponts
  • Induction medium and methods for stem cell culture and therapy
    Patent number: 11046929
    Abstract: Novel MSC stem-cell culture and therapy methods and culture medium compositions for the purpose of inducing, activating, or priming discrete uniform cell phenotypes to selectively promote or suppress inflammation and immunity, yielding polarized, primed, activated, or induced cells used in cell-based therapy.
    Type: Grant
    Filed: June 6, 2018
    Date of Patent: June 29, 2021
    Assignee: SanBio, Inc.
    Inventor: Aline Betancourt
  • Procedure for the production of a multiplier and modulator additive of the ruminal microbiote
    Patent number: 11044925
    Abstract: The present invention discloses a multiplier and modulator additive of the ruminal microbiota created from exogenous multienzyme compounds, integrating processes and devices for cultivating and harvesting biomass of selected fungal species, assembling active components of fungal origin and by mixing supplies according to specifications, forming thus a multiplier and modulator additive of the ruminal microbiota suitable for its application (as part of food or via distribution devices as a nutritional supplement) in the ruminant animal feed industry.
    Type: Grant
    Filed: September 11, 2018
    Date of Patent: June 29, 2021
    Assignee: BIOPREMIX TECHNOLOGIES LLC
    Inventor: Marcelo Alejandro Paternó
  • System and substances for cryopreservation of viable cells
    Patent number: 11028368
    Abstract: An improved cryopreservation process and substances can involve a cellular collection (1) in a cryopreservation fluid (4) that has been conditioned or treated (7) to enhance the cryopreservation process by adding (18) energy (19) such as in the surface energy of a substance in the cryopreservation fluid (4) prior to reducing energy for that same cryopreservation media for freezing. This can offer enhanced-post-cryogenic viability of the cryopreserved structures or a more optimum cooling curve (22) for a specific cell type.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: June 8, 2021
    Assignee: Membrane Protective Technologies, Inc.
    Inventors: Lisa A. Herickhoff, Patrick D. Burns, Nicole R. White, James A. Herickhoff
  • Method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid
    Patent number: 11015172
    Abstract: The invention relates to a method for culturing a subpopulation of circulating epithelial tumour cells from a body fluid of a human or animal suffering from an epithelial tumour, wherein cells contained in the body fluid each containing at least one cell nucleus are separated from the body fluid and cultured over at least 24 hours in suspension, with formation of spheroids.
    Type: Grant
    Filed: August 2, 2013
    Date of Patent: May 25, 2021
    Inventors: Ulrich Pachmann, Katharina Pachmann
  • Immunomodulatory properties of MAPCs and uses thereof
    Patent number: 11000546
    Abstract: Isolated cells are described that are not embryonic stem cells, not embryonic germ cells, and not germ cells. The cells can differentiate into at least one cell type of each of at least two of the endodermal, ectodermal, and mesodermal lineages. The cells do not provoke a harmful immune response. The cells can modulate immune responses. As an example, the cells can suppress an immune response in a host engendered by allogeneic cells, tissues, and organs. Methods are described for using the cells, by themselves or adjunctively, to treat subjects. For instance, the cells can be used adjunctively for immunosuppression in transplant therapy. Methods for obtaining the cells and compositions for using them also are described.
    Type: Grant
    Filed: May 3, 2018
    Date of Patent: May 11, 2021
    Assignees: ATHERSYS, INC., OREGON HEALTH & SCIENCE UNIVERISTY
    Inventors: Robert Deans, Richard Maziarz, Magdalena Kovacsovics, Philip Streeter, Wouter Van't Hof
  • Apparatus and method for detection of tumour cells and circulating tumour cells
    Patent number: 10987669
    Abstract: The invention relates to an apparatus and method of detecting and quantifying the number of circulating tumour cells (CTCs) and/or tumour cells (TCs) from a liquid biopsy by using a hyperoxic environment and incubation with a fluorophore-labelled metabolic indicator (fluorophore-labelled 2-D-glucose derivative) and microfluidic chips.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: April 27, 2021
    Assignees: MEDCOM TECH, S.A., FUNDACION DE INVESTIGACION HM HOSPITALES, MEDCOM ADVANCE, S.A.
    Inventors: Hainan Xie, Manuel Garcia Algar, Moritz Nazarenus, Juan Sagales, Carlos Villanueva Leal, Sara Gomez de Pedro, Eduardo Manuel Garcia Rico
  • Method of processing organic matter
    Patent number: 10982233
    Abstract: A process and system for treating biodegradable waste is described that utilizes serial thermophilic and mesophilic microbial digestion to product fuel gas and a stable solid product at high efficiencies.
    Type: Grant
    Filed: November 7, 2017
    Date of Patent: April 20, 2021
    Assignee: EAGLE GREEN ENERGY II LLC
    Inventors: John William Logan, Sumesh M Arora, Richard L Vetter, Eric Francis Shafer