Patents Examined by Taeyoon Kim
  • Method for generating pancreatic bud cells and therapeutic agent for pancreatic disease containing pancreatic bud cells
    Patent number: 10472610
    Abstract: Provided is a method for generating pancreatic bud cells, having the step of culturing PDX1+/NKX6.1? cells in a medium containing KGF, EGF and a BMP inhibitor. The culturing step may be performed in suspension cultures or in adherent cultures. When the cells are cultured in adherent cultures, the cells may be cultured in a medium further containing a ROCK inhibitor or a nonmuscle myosin II inhibitor.
    Type: Grant
    Filed: May 20, 2015
    Date of Patent: November 12, 2019
    Assignee: KYOTO UNIVERSITY
    Inventors: Kenji Osafune, Taro Toyoda
  • Generation of a mesenchymal stromal cell bank from the pooled mononuclear cells of multiple bone marrow donors
    Patent number: 10472608
    Abstract: The present invention pertains to an improved mesenchymal stromal cell (MSC) preparation and a method for producing the same. The invention provides a new strategy to isolate MSC from bone marrow mononuclear cells (BM-MNCs) by pooling BM-MNCs of multiple unrelated (third-party) bone marrow donors. The MSC preparation manufactured in accordance with the methodology of the invention is characterized by a stable proliferative capability and an increased immunosuppressive potential when compared to individual donor MSC preparations or a pool of individual MSCs generated from multiple donors. The MSCs prepared ac- cording to the invention are particularly useful for medical applications such as the treatment of graft-versus-host disease (GvHD) in recipients with hematopoietic stem cell transplants, patients with autoimmune disorders and as a cell-based therapy in regenerative medicine.
    Type: Grant
    Filed: July 14, 2015
    Date of Patent: November 12, 2019
    Assignees: JOHANN WOLFGANG GOETHE—UNIVERSITÄT, DRK BLUTSPENDEDIENST BADEN-WÜRTTEMBERG-HESSEN GGMBH
    Inventors: Peter Bader, Selim Kuci, Zyrafete Kuci, Halvard Bönig
  • Management of ischemia using pooled mesenchymal stromal cell composition
    Patent number: 10471101
    Abstract: The present disclosure relates to composition comprising pooled and expanded allogeneic mesenchymal Stromal cells (MSCs) and a method for management of Ischemia using the composition thereof. In particular, the disclosure relates to bone marrow derived pooled and expanded allogeneic MSC compositions with effective dosage ranges and modes/route of administration for effective management of Ischemia. The disclosure also relates to the use of conditioned medium rich in bioactive factors in combination with the cell composition for managing ischemic conditions.
    Type: Grant
    Filed: March 25, 2015
    Date of Patent: November 12, 2019
    Assignee: Stempeutics Research PVT. LTD.
    Inventors: Anish Sen Majumdar, Pawan Kumar Gupta, Anoop Chullikana House, Sudha Balasubramanian, Charan Thej, Mathiyazhagan Rengasamy
  • Method of screening a candidate compound for inducing bile canalicular function disorders
    Patent number: 10444228
    Abstract: In vitro methods and kits for modulating and studying mechanical movement of hepatic bile canaliculi lumen through activation or inhibition of the Rho-kinase molecular regulation pathway. In vitro methods and kits for modulating lumen opening and clearing using matrix metalloproteinases, as well as diagnostic methods based upon the same.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: October 15, 2019
    Assignee: Biopredic International
    Inventor: Christiane Guillouzo
  • Method for the clarification of high-density crude cell culture harvest
    Patent number: 10421986
    Abstract: The disclosure provides methods for the clarification of a cell broth with high cell density.
    Type: Grant
    Filed: September 29, 2014
    Date of Patent: September 24, 2019
    Assignee: Janssen Vaccines & Prevention B.V.
    Inventors: Ann-Marie De Villiers, Charles Maria Hubert Hensgens
  • Topical formulation for skin care
    Patent number: 10420802
    Abstract: Disclosed herein is a method of preparing a subject's cells for topical treatment of a subject in need. Also provided is a topical formulation and methods of treating a subject suffering a skin disorder and pain using the topical formulation. Also disclosed herein are devices and systems for micronizing an aspirate in preparation of such a topical formulation.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: September 24, 2019
    Assignee: AESTHETICS BIOMEDICAL, INC.
    Inventor: Farhan Taghizadeh
  • Methods for improving fertility and selectivity for desired offspring sex in artificial insemination
    Patent number: 10416152
    Abstract: A method for providing semen optimized for use in artificial insemination is described. Methods involve monitoring sperm cell metabolism by assays that produce results in real time, while sperm are still being processed into doses for use in insemination. Processing is modified in response to assay results, to optimize sperm performance.
    Type: Grant
    Filed: June 17, 2016
    Date of Patent: September 17, 2019
    Assignee: AREX LIFE SCIENCES, LLC
    Inventor: Barb A. Cohen
  • Method of fermenting mycelium composite material
    Patent number: 10407675
    Abstract: A method of fermenting mycelium composite materials wherein layers of fermentable material are stacked in alteration with ventilation layers with air being passed through the ventilation layers to remove heat and gas generated in the layers of fermentable material during fermentation thereof. The obtained composite materials may be formed into cohesive flat boards, such as are used in the manufacture of insulation or furniture.
    Type: Grant
    Filed: March 6, 2017
    Date of Patent: September 10, 2019
    Assignee: Ecovative Design LLC
    Inventors: Eben Bayer, Peter Mueller, Christopher Scully
  • Stem cell composition
    Patent number: 10400218
    Abstract: The disclosure provides stem cells which express high levels of Angeopoetin-1 (Ang1) and methods for their production. Such stem cells may be used in a range of therapeutic application.
    Type: Grant
    Filed: April 7, 2015
    Date of Patent: September 3, 2019
    Assignee: MESOBLAST INTERNATIONAL SARL
    Inventors: Silviu Itescu, Paul Simmons
  • Methods and compositions for delivering interleukin-1 receptor antagonist
    Patent number: 10400017
    Abstract: Methods and compositions generating and using an interleukin-1 receptor antagonist (IL-1ra)-rich solution. Methods for generating and isolating interleukin-1 receptor antagonist include incubating a liquid volume of white blood cells and platelets with polyacrylamide beads to produce interleukin-1 receptor antagonist. The interleukin-1 receptor antagonist is isolated from the polyacrylamide beads to obtain the solution rich in interleukin-1 receptor antagonist. Methods for treating a site of inflammation in a patient include administering to the site of inflammation the solution rich in interleukin-1 receptor antagonist.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: September 3, 2019
    Assignee: Biomet Biologics, LLC
    Inventors: Joel C. Higgins, Jennifer E. Woodell-May, Jacy C. Hoeppner
  • Method for in vivo hair multiplication
    Patent number: 10391200
    Abstract: Cosmetic methods providing a significant improvement over existing hair transplantation methods by providing greater hair density per hair implant, which is achieved through the process of an enhanced hair multiplication in vivo (e.g. in the scalp of a subject). The cosmetic methods also provide improved aesthetic results, particularly, more realistic and natural looking results, relative to the results obtained by traditional methods. The cosmetic methods are particularly suitable for hair transplantation in recipient areas of a subject experiencing baldness or lack of hair, optionally due to Androgenic alopecia, burn injuries, cancer chemotherapy, or other genetic or environmental factors or scarring.
    Type: Grant
    Filed: December 12, 2013
    Date of Patent: August 27, 2019
    Assignee: HAIR SCIENCE INSTITUTE
    Inventor: Conradus Ghosal Gho
  • Isolation of human lung progenitors derived from pluripotent stem cells
    Patent number: 10386368
    Abstract: Provided herein are methods and compositions relating, in part, to the generation and isolation of human lung progenitor cells from pluripotent stem cells.
    Type: Grant
    Filed: February 22, 2018
    Date of Patent: August 20, 2019
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Darrell N. Kotton, Finn Hawkins
  • Antimicrobial compositions and related methods of use
    Patent number: 10383332
    Abstract: Antimicrobial compositions comprising one or more compound components generally recognized as safe for human consumption, and related methods of use, such compositions and methods as can be employed in a wide range of agricultural, industrial, building, pharmaceutical, personal care and/or animal care products and applications.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: August 20, 2019
    Assignee: Jeneil Biosurfactant Company, LLC
    Inventors: Niranjan Ramanlal Gandhi, Victoria Palmer Skebba, Gary A. Strobel
  • Histological specimen treatment
    Patent number: 10365189
    Abstract: A single dissolving compound forms plural azeotropes, which can be azeotropically vaporized off at various stages of the treatment process, thus maintaining predictable concentrations of the chemicals present. The treatment process can be performed in the absence of formalin or related compounds which can interfere with the preservation of genetic material. A process for preserving a specimen includes using a dissolving compound that can form a plural number of azeotropes, at least one azeotrope being formed between one or more components of the dissolving compound and specimen-supplied water, and at least one azeotrope being formed between different components of the dissolving compound; successively and azeotropically vaporizing off formed azeotropes; and impregnating the specimen with a support medium.
    Type: Grant
    Filed: May 7, 2015
    Date of Patent: July 30, 2019
    Inventor: Steven Wheeler
  • Methods for identifying modulators of membrane potentials in bipolar disorder and attention deficit hyperactivity disorder
    Patent number: 10365268
    Abstract: The present invention provides methods to modulate key elements along the DAG signaling pathway as well as a diagnostic assay, device and methods of using the same to diagnose bipolar disorder (BD) and attention deficit hyperactivity disorder (ADHD). Methods to identify diagnostic markers and drug targets for BD and ADHD. Methods of identifying effective compounds responsible for membrane potentials and excitabilities influencing bipolar disorder (BD) and attention deficit hyperactivity disorder (ADHD). Methods of identifying an effective compound that modulates the activity of Ca2+/CaM enzyme and compounds involved in changing the K+ gradient across the plasma membrane thereby increasing or decreasing the membrane potential ratio (MPR™) values. The invention provides methods of identifying a compound that modulates the activity of PKC which is an important protein of the DAG signaling pathway.
    Type: Grant
    Filed: September 26, 2016
    Date of Patent: July 30, 2019
    Assignee: PsychNostics, LLC
    Inventor: Alagu P. Thiruvengadam
  • Isolation, cultivation and uses of stem/progenitor cells
    Patent number: 10363275
    Abstract: The present invention relates to a method for isolating stem/progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem/progenitor cells from the tissue cultures. The isolated stem cell cells can have embryonic stem cell-like properties and can be used for various therapeutic purposes. In one embodiment, the invention relates to the isolation and cultivation of stem cells such as epithelial and/or mesenchymal stem/progenitor cells under conditions allowing the cells to undergo mitotic expansion. Furthermore, the invention is directed to a method for the differentiation of the isolated stem/progenitor cells into epithelial and/or mesenchymal cells.
    Type: Grant
    Filed: August 15, 2005
    Date of Patent: July 30, 2019
    Assignee: CellResearch Corporation Pte Ltd
    Inventors: Toan-Thang Phan, Ivor Jiun Lim
  • Cell populations which co-express CD49c and CD90
    Patent number: 10351826
    Abstract: Substantially homogenous cells populations which co-express CD49c, CD90 and telomerase are made. In one embodiment, humans suffering from a degenerative, traumatic, acute injury, cardiac or neurological condition are treated with the substantially homogenous cells populations which co-express CD49c, CD90 and telomerase. In another embodiment, committed progenitor cells are made are made by selecting from a cultured source of a cell population which co-express CD49c and CD90 and modifying the cell population. The committed progenitor cells can be employed to treat a human suffering from a degenerative, traumatic, acute injury, cardiac or neurological condition and formulate pharmaceutical compositions.
    Type: Grant
    Filed: May 2, 2007
    Date of Patent: July 16, 2019
    Assignee: GARNET BIOTHERAPEUTICS, INC.
    Inventors: Tony W. Ho, Gene C. Kopen, William F. Righter, J. Lynn Rutkowski, Joseph Wagner
  • Enamel products and methods of use
    Patent number: 10350147
    Abstract: Described herein are three-dimensional (3D) culture systems that can be used to produce enamel organoids that generate enamel products. The invention features methods of culturing a variety of cell types to produce such enamel organoids; the organoids themselves; enamel products generated by the organoids; and methods of fashioning the enamel products into surgical restorations, including dental restorations and other prostheses.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: July 16, 2019
    Assignee: The Research Foundation for The State University of New York
    Inventors: Stephanos Kyrkanides, Li Ma, Sabine Brouxhon
  • Method for producing human corneal epithelium sheet
    Patent number: 10335439
    Abstract: [Problem] To provide a method for producing human corneal epithelial sheet, wherein the human corneal epithelial-derived cells obtained by culturing human corneal epithelial cells are cultured on an amnion substrate. [Solution] A method for culturing human corneal epithelial cells using mesenchymal stem cells as the feeder cells; and a method for culturing human corneal epithelial cells using a medium containing a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor and a TGF-? receptor inhibitor in various combinations.
    Type: Grant
    Filed: March 9, 2014
    Date of Patent: July 2, 2019
    Assignees: JCR Pharmaceuticals Co., Ltd., Kyoto Prefectural Public University Corporation
    Inventors: Kiwamu Imagawa, Kenichi Maeda, Yuki Hosoda, Shuichi Yokoyama, Naoki Okumura, Noriko Koizumi, Shigeru Kinoshita
  • System and method for sequestering substances in bulk liquids
    Patent number: 10329554
    Abstract: Systems and methods for detecting and sequestering target analytes in a fluid sample. The system comprises a bulk specimen reservoir for receiving a liquid sample, along with paramagnetic materials operative to selectively bind to the target analyte of interest. A vertex is interconnected with the bulk specimen reservoir to define a specimen chamber assembly to which a magnetic chamber is deployed about the vertex portion thereof and operative to sequester the magnetic particles, as well as any analyte of interest bound thereto, within the interior of the vertex. Once sufficient time is allowed for mixing and allowing the magnetic capsule to sufficiently retain the magnetic particles within the vertex, the vertex is disconnected from the specimen reservoir and the paramagnetic materials retained therein analyzed to determine the presence of the analyte of interest.
    Type: Grant
    Filed: November 7, 2016
    Date of Patent: June 25, 2019
    Assignee: WAVESENSE, INC.
    Inventor: Christopher Feistel