Patents Examined by Tekchand Saidha
  • Engineered enzyme
    Patent number: 11214781
    Abstract: The present disclosure provides a novel catalytically inactive MAD7 nuclease (dMAD7) that retains the ability to bind DNA in a sequence-specific manner. The MAD7 nuclease from which the dMAD7 has been derived was isolated from Eubacterium rectale.
    Type: Grant
    Filed: July 13, 2021
    Date of Patent: January 4, 2022
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts
  • Tripterygium wilfordii cryptomeridiol synthase, coding gene thereof and recombinant yeast containing coding gene
    Patent number: 11214788
    Abstract: Provided are a Cryptomeridiol synthase and a coding gene thereof. Also provided are a Cryptomeridiol synthase and a coding gene, a engineered yeast containing the Cryptomeridiol coding gene, and a use of same in plant breeding and biosynthesis. The cDNA full-length sequence of the Cryptomeridiol synthase gene in Tripterygium wilfordii is obtained by means of polymerase chain reaction cloning. Then, by means of synthetic biology, the engineered yeast containing the Cryptomeridiol synthase gene is constructed to realize the production of Cryptomeridiol in the yeast.
    Type: Grant
    Filed: January 30, 2019
    Date of Patent: January 4, 2022
    Assignee: CAPITAL MEDICAL UNIVERSITY
    Inventors: Wei Gao, Luqi Huang, Yuru Tong, Tianyuan Hu
  • Method for biocatalytic production of terpene compounds
    Patent number: 11214775
    Abstract: Provided herein are biocatalytic methods of producing terpene compounds by applying a novel type of phosphatase enzyme. The method allows the fully biochemical synthesis of terpene compounds, like for example copalol and labdendiol, and derivatives thereof, which serve as valuable intermediates for the production of perfumery ingredients, such as, for example, ambrox. Also provided are novel fully biochemical multistep processes for the production of such compounds as well as novel phosphatase enzymes and mutants and variants derived therefrom.
    Type: Grant
    Filed: July 10, 2019
    Date of Patent: January 4, 2022
    Inventors: Michel Schalk, Letizia Rocci, Daniel Solis Escalante
  • Microbial syringol utilization
    Patent number: 11208642
    Abstract: Disclosed herein are compositions of non-naturally occurring enzymes to enable microbial syringol utilization with GcoAB.
    Type: Grant
    Filed: November 14, 2019
    Date of Patent: December 28, 2021
    Assignees: Alliance for Sustainable Energy, LLC, Montana State University, University of Portsmouth
    Inventors: Gregg Tyler Beckham, Jennifer DuBois, Melodie M. Machovina, Simon James Bradshaw Mallinson, John E. McGeehan, Christopher W. Johnson, Alexander William Meyers
  • Variants of terminal deoxynucleotidyl transferase and uses thereof
    Patent number: 11208637
    Abstract: The present invention relates to variants of Terminal deoxynucleotidyl Transferase (TdT), each of which (i) has an amino acid sequence similarity to SEQ ID NO: 2. 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 with corresponding amino acid substitutions, (ii) is capable of synthesizing a nucleic acid fragment without a template and (iii) is capable of incorporating a modified nucleotide into the nucleic acid fragment.
    Type: Grant
    Filed: July 10, 2020
    Date of Patent: December 28, 2021
    Assignees: DNA Script SAS, Institut Pasteur, National Center for Scientific Research (CNRS)
    Inventors: Elise Champion, Mikhael Soskine, Thomas Ybert, Marc Delarue
  • Riboswitch-mediated regulatory control of gene expression in thermophilic bacteria
    Patent number: 11198871
    Abstract: Methods of riboswitch-mediated gene expression in thermophiles are provided herein.
    Type: Grant
    Filed: February 17, 2020
    Date of Patent: December 14, 2021
    Assignee: Alliance for Sustainable Energy, LLC
    Inventors: Joan Gabriel Marcano, Katherine Jenyan Chou
  • Yeast cell capable of simultaneously fermenting hexose and pentose sugars
    Patent number: 11198847
    Abstract: The invention relates to a method for preparing a yeast which is capable of simultaneously fermenting a pentose and a hexose sugar, the method comprising providing a yeast which comprises: one or more heterologous genes encoding an enzyme of a pentose metabolic pathway, a disruption of a gene encoding a ribulose-phosphate 3-epimerase and a disruption of a gene encoding a glucose-6-phosphate isomerase, and one or more overexpressed endogenous genes encoding an enzyme of the pentose phosphate pathway; and subjecting the yeast to evolutionary engineering on a medium comprising a hexose sugar and at least one pentose sugar, selecting for a yeast with improved growth rate when grown on a media comprising a hexose and at least one pentose sugar, so as to obtain an evolved yeast.
    Type: Grant
    Filed: January 31, 2019
    Date of Patent: December 14, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Maarten Dirk Verhoeven, Ioannis Papapetridis
  • Yeast expressing heterologous glucoamylase
    Patent number: 11198881
    Abstract: The present disclosure concerns recombinant yeast host cell for saccharification of a biomass. The recombinant yeast host cell has a genetic modification for expressing a heterologous polypeptide having glucoamylase activity (Rasamsonia emersonii glucoamylase). In some embodiments, the heterologous polypeptide comprises the signal sequence associated with the alpha-mating 1 factor. The present disclosure also concerns a process for saccharification of a biomass using the recombinant yeast host cell as well as a process for fermenting the saccharified biomass into a fermentation product.
    Type: Grant
    Filed: November 25, 2020
    Date of Patent: December 14, 2021
    Assignees: Lallemand Hungary Liquidity Management LLC, BASF SE
    Inventors: Aaron Argyros, Alexandra Panaitiu, Charles Rice, Kenneth Barrett
  • Processes using amino acid dehydrogenases and ketoreductase-based cofactor regenerating system
    Patent number: 11193157
    Abstract: The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase.
    Type: Grant
    Filed: February 21, 2020
    Date of Patent: December 7, 2021
    Assignee: Codexis, Inc.
    Inventors: Fabien Louis Cabirol, Steven J. Collier, Thomas Daussmann, Naga K. Modukuru
  • Yeast strain having improved capability for fermenting xylose in the presence of acetic acid
    Patent number: 11186814
    Abstract: The invention relates to a method for selecting a yeast strain having improved capability for fermenting a pentose, advantageously xylose, in the presence of organic acid, advantageously acetic acid, in non-dissociated form, in which at least one yeast strain that is capable of fermenting said pentose is consecutively cultured in the following two media: a first growth medium comprising said pentose as the only carbon source and said organic acid in non-dissociated form; a second growth medium comprising another carbon source as the only carbon source, advantageously glucose, free of said organic acid in non-dissociated form, the consecutive culture in at least said two growth media being repeated at least twice, in the presence of rising concentrations of organic acid in non-dissociated form.
    Type: Grant
    Filed: February 18, 2020
    Date of Patent: November 30, 2021
    Assignee: LESAFFRE et COMPAGNIE
    Inventors: Jean-Michel Bavouzet, Thomas Desfougeres, Georges Pignede, Jennifer Techel
  • Biosynthesis of products from 1-carbon compounds
    Patent number: 11186834
    Abstract: An engineered microbe that contains a designed platform for the conversion of one-carbon substrates to chemical products is described. The designed platform embodies a new metabolic architecture that consolidates carbon fixation, central metabolism, and product synthesis into a single pathway. This is made possible by the key finding that 2-hydroxyacyl-CoA lyase, an enzyme in the ?-oxidation pathway, is capable of catalyzing the C—C bond formation between formyl-CoA and aldehydes of different chain lengths, allowing for the elongation of the carbon backbone of said aldehyde by one-carbon units. These novel microbes present an opportunity for the production of chemicals from single-carbon feedstocks such as carbon dioxide, carbon monoxide, formate, formaldehyde, methanol or methane.
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: November 30, 2021
    Inventors: Ramon Gonzalez, Alexander Chou, James Clomburg
  • Mixtures and compositions comprising strains or metabolites thereof and other biopesticides
    Patent number: 11185078
    Abstract: The present invention relates to novel mixtures comprising, as active components, at least one isolated bacterial strain, which is a member of the genus Paenibacillus, or a cell-free extract thereof or at least one metabolite thereof, and at least one other biopesticide. The present invention also relates to compositions comprising at least one of such bacterial strains, whole culture broth or a cell-free extract or a fraction thereof or at least one metabolite thereof, and at least one other biopesticide. The present invention also relates to a method of controlling or suppressing plant pathogens or preventing plant pathogen infections by applying such composition. The present invention also relates to mixtures of fusaricidins which are pesticidal metabolites produced by the abovementioned strains, and other biopesticides.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: November 30, 2021
    Assignee: BASF SE
    Inventors: Isabella Siepe, Thorsten Jabs, Burghard Liebmann, Annette Schuster
  • Mutant pore
    Patent number: 11186868
    Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: November 30, 2021
    Assignee: Oxford Nanopore Technologies PLC
    Inventors: Lakmal Jayasinghe, Elizabeth Jayne Wallace
  • Transformed fungus having enhanced ergothioneine productivity and method for producing ergothioneine
    Patent number: 11186855
    Abstract: The purpose of the present invention is to provide an organism having an ergothioneine productivity that is capable of easily producing ergothioneine within a short period of time at a high yield, as compared with a conventional technology, and, therefore, enables ergothioneine production on an industrial scale. This purpose can be achieved by a transformed fungus into which a gene encoding enzyme (1) or genes encoding enzymes (1) and (2) have been inserted and in which the inserted gene(s) are overexpressed. (1) an enzyme catalyzing a reaction of synthesizing hercynyl cysteine sulfoxide from histidine and cysteine in the presence of S-adenosyl methionine, iron (II) and oxygen. (2) An enzyme catalyzing a reaction of synthesizing ergothioneine from hercynyl cysteine sulfoxide using pyridoxal 5?-phosphate as a coenzyme.
    Type: Grant
    Filed: April 27, 2020
    Date of Patent: November 30, 2021
    Assignee: KIKKOMAN CORPORATION
    Inventors: Seiichi Hara, Keiko Kurosawa, Keiichi Ichikawa
  • Increased production of ginsenosides through yeast cell organelle improvement
    Patent number: 11186616
    Abstract: Provided are a recombinant yeast having improved ability to produce ginsenoside, which is prepared by overexpressing INO2 and INO4 or deleting OPT1 in a yeast having ability to produce ginsenoside, a method of preparing the yeast, and a method of producing ginsenoside by using the yeast.
    Type: Grant
    Filed: September 21, 2020
    Date of Patent: November 30, 2021
    Assignee: KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY
    Inventors: Ju Young Lee, In Seung Jang, Seohyun Kim, Sun-Chang Kim, Suk Chea Jung, Jong Geon Jegal
  • Methods and compositions for producing hydrocarbons
    Patent number: 11186854
    Abstract: Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.
    Type: Grant
    Filed: January 7, 2020
    Date of Patent: November 30, 2021
    Assignee: Genomatica, Inc.
    Inventors: Andreas W. Schirmer, Mathew A. Rude, Shane A. Brubaker
  • GH10 xylanase, GH62 arabinofuranosidase, milling process and other application
    Patent number: 11180786
    Abstract: The present invention provides an improved process of treating crop kernels to provide a starch product of high quality suitable for conversion of starch into mono- and oligosaccharides, ethanol, sweeteners. The present invention also provides polypeptides having GH10 xylanase activity, polypeptides having GH62 arabinofuranosidase activity and the uses thereof.
    Type: Grant
    Filed: November 24, 2017
    Date of Patent: November 23, 2021
    Assignee: Novozymes A/S
    Inventors: Yi Cao, Yu Zhang, Bernardo Vidal, Jr., Randall Scott Deinhammer, Mary Ann Stringer, Ye Liu, Purna Venkatesh, Cui Liu
  • System and method of biocatalytic conversion for production of alcohols, ketones, and organic acids
    Patent number: 11180779
    Abstract: Biocatalytic conversion systems and methods of producing and using same that have improved yields are disclosed. The systems and methods involve co-fermentation of sugars and gaseous substrates for alcohol, ketone, and/or organic acid production. The systems and methods may include biocatalytically converting at least one sugar substrate into at least one of alcohol, at least one ketone, and/or at least one organic acid. The systems and methods may further include biocatalytically converting gases that comprise CO2 and H2 to at least one alcohol and/or at least one organic acid, thereby adding extra revenue to biorefineries.
    Type: Grant
    Filed: April 12, 2018
    Date of Patent: November 23, 2021
    Assignee: The Board of Regents for Oklahoma State University
    Inventor: Hasan K. Atiyeh
  • Organisms for the production of 1,3-butanediol
    Patent number: 11180780
    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: November 23, 2021
    Assignee: Genomatica, Inc.
    Inventors: Anthony P. Burgard, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
  • Method for producing chiral amines
    Patent number: 11180783
    Abstract: A method for producing a chiral amine may include performing a transamination reaction of a prochiral amino acceptor and an amino donor in a first solution in the presence of a transaminase, thereby forming a chiral amine and a co-product in the first solution. The amino donor is a high molecular weight (HMW) amino donor. In some examples, the molecular weight of the HMW amino donor is at least 150 g/mol. In some examples, the amino donor is affixed on a support, the total molecular weight of the amino donor and the support being at least 150 g/mol.
    Type: Grant
    Filed: August 31, 2018
    Date of Patent: November 23, 2021
    Assignee: VITO NV
    Inventors: Yamini Satyawali, Dominic Ormerod, Claudia Matassa, Karolien Vanbroekhoven