Abstract: The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.
Type:
Grant
Filed:
August 13, 2020
Date of Patent:
June 7, 2022
Assignee:
Manus Bio Inc.
Inventors:
Ajikumar Parayil Kumaran, Ryan Lim, Jason Donald, Hsien-Chung Tseng, Christine Nicole S. Santos, Ryan Philippe
Abstract: DGA1 catalyzes the final enzymatic step for converting acyl-CoA and 1,2-diacylglycerol to triacylglycerols (TAG) and CoA in yeast. Disclosed are methods for expression in an oleaginous yeast host of polynucleotide sequences encoding DGA1 from Rhodosporidium toruloides, Lipomyces starkeyi, Aurantiochytrium limacinum, Aspergillus terreus, or Claviceps purpurea. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising heterologous DGA1 polynucleotides encoding DGA1 proteins, or functionally active portions thereof, having the capability of producing increased lipid production and possessing the characteristic of enhanced glucose consumption efficiency.
Type:
Grant
Filed:
February 28, 2019
Date of Patent:
June 7, 2022
Assignee:
Ginkgo Bioworks, Inc.
Inventors:
Elena E. Brevnova, Arthur J. Shaw, IV, Emily H. Greenhagen
Abstract: The invention relates to a process for the preparation of EPA and DHA enriched glycerides from degummed oil using microbial lipase enzymes. It particularly relates to the use of very less concentrations of said enzymes and water for the hydrolysis process. Used lipase enzymes specifically removes non-EPA/DHA fatty acids which results in enriched and balanced content of EPA/DHA in to the final product.
Abstract: The present invention relates to oleaginous yeast cells for the production of fatty alcohols and derivatives thereof, in particular desaturated fatty alcohols, desaturated fatty acyl acetates and desaturated fatty aldehydes. Also provided are methods for obtaining such compounds, which are useful in pheromone compositions.
Type:
Grant
Filed:
December 15, 2017
Date of Patent:
May 31, 2022
Assignee:
Danmarks Tekniske Universitet
Inventors:
Irina Borodina, Carina Holkenbrink, Marie Inger Dam, Christer Löfstedt
Abstract: The present disclosure generally relates to modified microorganisms comprising an optimized system for oligosaccharide utilization comprising one or more polynucleotides coding for one or more energy independent oligosaccharide transporters for transporting an oligosaccharide into the microorganism, one or more polynucleotides coding for enzymes that catalyze the conversion of the oligosaccharide into at least one phosphorylated saccharide, and one or more polynucleotides coding for enzymes that catalyze the conversion of the phosphorylated saccharide into an isomer of the phosphorylated saccharide that is utilized in one or more enzymatic pathways in the microorganism for the production of an organic molecule such as acetic acid, acrylic acid, 3-hydroxypropionic acid, lactic acid, etc. The present disclosure also generally relates to methods of using the optimized system for oligosaccharide utilization.
Type:
Grant
Filed:
May 29, 2015
Date of Patent:
May 31, 2022
Assignee:
BRASKEM S.A.
Inventors:
Avram Michael Slovic, Daniel Johannes Koch
Abstract: The disclosure relates to engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives including e.g., eight carbon and ten carbon fatty acids and fatty acid derivatives. The disclosure further relates to recombinant host cells comprising the engineered plant acyl-ACP thioesterases having improved activity for the production of medium-chain fatty acid derivatives. The disclosure also relates to methods of decreasing toxicity and improving production of medium-chain fatty acids and derivatives.
Type:
Grant
Filed:
April 3, 2018
Date of Patent:
May 24, 2022
Assignee:
Genomatica, Inc.
Inventors:
Angelica Zabala Bautista, Min Sun Park, Alma Itzel Ramos-Solis, Bernardo Moura Torres Da Costa, Behnaz Behrouzian, Donald E. Trimbur, Emanuela Popova, Qijun Hu, Stephen Bralley Del Cardayre
Abstract: The present disclosure relates to a microorganism of the genus Corynebacterium producing 5?-xanthosine monophosphate and a method for producing 5?-xanthosine monophosphate using the same.
Type:
Grant
Filed:
October 21, 2020
Date of Patent:
May 17, 2022
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Min Ji Baek, Ji Hye Lee, Boram Lim, Byoung Hoon Yoon, Jeong Eun Lee, Su-bin Lim, Jaeho Jeong
Abstract: The present invention relates to variants of a parent lipase which has lipase activity and comprise one or more substitutions corresponding to G23S, D27N, A40I, F51I,L, E56R, D57N, V60E,K, K98I, N101D, R118F, G163S, T231R, N233R, Y220F, T244E, and P256T using SEQ ID NO: 2 for numbering. The present invention also relates to compositions or microcapsule compositions comprising a lipase variant of the invention and to liquid products comprising a microcapsule composition of the invention as well as the use of said microcapsule composition for stabilizing lipase variants of the invention.
Type:
Grant
Filed:
September 25, 2018
Date of Patent:
May 17, 2022
Assignee:
Novozymes A/S
Inventors:
Miguel Duarte Guilherme Pereira Toscano, Thomas Agersten Poulsen, Carsten Horslev Hansen, Lone Baunsgaard, Keith Gibson
Abstract: The present invention relates to a yeast cell that is genetically modified comprising: a) a disruption of one or more aldehyde dehydrogenase (E.C:1.2.1.4) native to the yeast; b) one or more nucleotide sequence encoding a heterologous NAD+-dependent acetylating acetaldehyde dehydrogenase (E.C. 1.2.1.10); c) one or more nucleotide sequence encoding a homologous or heterologous acetyl-CoA synthetase (E.C. 6.2.1.1); and d) a modification that leads to reduction of glycerol 3-phosphate phosphohydrolase (E.C. 3.1.3.21) and/or glycerol 3-phosphate dehydrogenase (E.C. 1.1.1.8 or E.C. 1.1.5.3) activity, native to the yeast.
Type:
Grant
Filed:
May 18, 2016
Date of Patent:
May 3, 2022
Assignee:
DSM IP ASSETS B.V.
Inventors:
Paul Klaassen, Jozef Petrus Johannes Schmitz, Paulus Petrus De Waal, Arjen Bouma
Abstract: Methods of using microorganisms to make chemicals and fuels, including carboxylic acids, alcohols, hydrocarbons, and their alpha-, beta-, and omega-functionalized derivatives are described. Native or engineered thiolases are used condense a growing acyl-ACP and acetyl-ACP in combination with type II fatty acid synthesis. The resulting fatty acid biosynthesis cycle has an ATP yield analogous to the functional reverse ?-oxidation cycle.
Abstract: The present invention relates to detergent compositions comprising a variant of a parent lipase which variant has lipase activity, has at least 60% but less than 100% sequence identity with SEQ ID NO: 2, and comprises substitutions at positions corresponding to T231R+N233R and at least one or more (e.g., several) of D96E, D111A, D254S, G163K, P256T, G91T, and G38A of SEQ ID NO: 2. The present invention also relates to use of the compositions, methods of producing the compositions, and methods of cleaning. The present invention furthermore relates to variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides.
Type:
Grant
Filed:
May 28, 2019
Date of Patent:
April 26, 2022
Assignee:
NOVOZYMES A/S
Inventors:
Marco Malten, Kim Borch, Lise Munch Mikkelsen, Thomas Agersten Poulsen, Luise Erlandsen, Hanne Philbert Nielsen, Carsten Horslev Hansen, Allan Svendsen, Christian Lundager Gylstorff, Jesper Vind, Christian Jackel
Abstract: Alteromonas macleodii Dow02-2 is provided, wherein the Alteromonas macleodii Dow02-2 was deposited at German Collection of Microorganisms and Cell Cultures (Deutsche Sammlung von Mikroorganismen and Zellkulturen, DSMZ) under the accession number DSM33380, and its metabolites comprise 2-hydroxy-3-methylbutyric acid. A method for providing 2-hydroxy-3-methylbutyric acid is also provided, wherein the method comprises isolating the 2-hydroxy-3-methylbutyric acid from metabolites of Alteromonas macleodii Dow02-2 of biologically pure culture. A method for at least one of increasing expression of TGM1 gene, increasing expression of KRT1 gene, increasing expression of KRT10 gene, increasing expression of KRT14 gene, increasing expression of FLG gene, increasing expression of GBA gene, and increasing expression of HAS3 gene is also provided, wherein the method comprises administering to a subject in need thereof an effective amount of 2-hydroxy-3-methylbutyric acid.
Type:
Grant
Filed:
December 19, 2019
Date of Patent:
April 19, 2022
Assignee:
TCI Co., Ltd.
Inventors:
Chu-Han Huang, Huan-You Lin, Cheng-Yu Ho, Yung-Hsiang Lin
Abstract: A cleaning agent for hard surfaces and methods for cleaning are provided herein. In one embodiment, the cleaning agent includes at least one first amylase, wherein the first amylase is an ?-amylase from Bacillus sp. No. 707 or a functional fragment or a variant thereof. The cleaning agent further includes at least one second amylase, wherein the second amylase is an AA560 ?-amylase from Bacillus sp. or a functional fragment or a variant thereof. In another embodiment, the method includes dispensing the cleaning agent into the interior of an automatic dishwasher while a dishwashing program is being executed, before the main washing cycle begins, or in the course of the main washing cycle.
Type:
Grant
Filed:
July 12, 2019
Date of Patent:
April 19, 2022
Assignee:
HENKEL AG & CO. KGAA
Inventors:
Thomas Eiting, Nina Mussmann, Thorsten Bastigkeit, Inga Kerstin Vockenroth, Noelle Wrubbel, Thomas Weber, Silke Menke
Abstract: Disclosed is a new tagatose 6-phosphate 4-epimerase, which is capable of converting fructose 6-phosphate into tagatose 6-phosphate and vice versa. Also disclosed is an application of the enzyme in tagatose production.
Type:
Grant
Filed:
December 28, 2018
Date of Patent:
April 19, 2022
Assignee:
TIANJIN INSTITUTE OF INDUSTRIAL BIOTECHNOLOGY, CHINESE ACADEMY OF SCIENCES
Inventors:
Yanhe Ma, Yuanxia Sun, Yunjie Li, Jiangang Yang
Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 29 or an amino acid sequence having at least about 50% sequence identity thereto.
Type:
Grant
Filed:
August 15, 2016
Date of Patent:
April 12, 2022
Assignee:
DSM IP ASSETS B.V.
Inventors:
Viktor Marius Boer, Priscilla Zwartjens, Eric Van Den Berg
Abstract: A recombinant host capable of producing a steviol glycoside which overexpresses a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto. A recombinant host capable of producing a steviol glycoside which has been modified, preferably in its genome, to result in a deficiency in the production of a polypeptide which mediates steviol glycoside transport and which polypeptide comprises the amino acid sequence set forth in SEQ ID NO: 35 or SEQ ID NO: 38 or an amino acid sequence having at least about 50% sequence identity to either thereto.
Type:
Grant
Filed:
April 10, 2020
Date of Patent:
April 5, 2022
Assignee:
DSM IP ASSETS B.V.
Inventors:
Viktor Marius Boer, Priscilla Zwartjens, Eric Van Den Berg
Abstract: The invention relates to a genetically modified microorganism for making a oligosaccharide, preferably of 3-8 monosaccharide units, more preferably of 3-5 monosaccharide units, particularly a HMO, which comprises one or more genes encoding a sucrose utilization system, so the microorganism can use sucrose as a carbon and energy source.
Type:
Grant
Filed:
July 13, 2020
Date of Patent:
April 5, 2022
Assignee:
GLYCOM A/S
Inventors:
Margit Pedersen, Manos Papadakis, Peter Becker, Eric Samain, Pauline Peltier-Pain, Katrine Bych, Ted Johanson, Elise Champion, Gyula Dekany