Patents Examined by Yong D. Pak
  • Patent number: 10435724
    Abstract: Improvement of C4 dicarboxylic acid productivity in a host cell. A polypeptide consisting of an amino acid sequence represented by SEQ ID No: 2 or an amino acid sequence having an identity of at least 90% with the amino acid sequence represented by SEQ ID No: 2.
    Type: Grant
    Filed: October 12, 2016
    Date of Patent: October 8, 2019
    Assignee: Kao Corporation
    Inventor: Jitsuro Kaneda
  • Patent number: 10435729
    Abstract: The present invention relates to the production of the disaccharide kojibiose which is known to be a powerful prebiotic. The invention indeed discloses the generation of genetically modified sucrose phosphorylases which convert—via a transglycosylation reaction—sucrose into kojibiose in a very efficient manner. Hence, the present invention relates to a cost-effective production method of kojibiose which is useful within industry.
    Type: Grant
    Filed: November 12, 2015
    Date of Patent: October 8, 2019
    Inventors: Tom Verhaeghe, Karel De Winter, Tom Desmet
  • Patent number: 10428364
    Abstract: Provided is a method for preparing rebaudioside M by using an enzyme method. In the method, rebaudioside A or rebaudioside D is used as a substrate; and in the existence of a glucosyl donor, rebaudioside M is generated by means of reaction of the substrate under the catalysis of UDP-glucosyl transferase and/or recombinant cells containing the UDP-glucosyl transferase.
    Type: Grant
    Filed: April 10, 2019
    Date of Patent: October 1, 2019
    Assignee: PEPSICO, INC.
    Inventors: Junhua Tao, Guoqing Li, Xiaoliang Liang, Andrew Tao
  • Patent number: 10415025
    Abstract: Provided are a fungus-sourced high-temperature acid ?-glucosidase as well as a coding gene, and an application thereof. The provide ?-glucosidase has the optimal pH value of 4.5 and the optimal temperature of 75° C., and maintains over 90% enzyme activity in the optimal condition after being processed at 60° C. for 1 h. The re-engineering yeast strain GS115/bgl3A of the coding gene comprising the ?-glucosidase has high fermentation level.
    Type: Grant
    Filed: December 8, 2014
    Date of Patent: September 17, 2019
    Inventors: Bin Yao, Pengjun Shi, Wei Xia, Huiying Luo, Huoqing Huang, Peilong Yang, Yaru Wang, Xiaoyun Su, Yingguo Bai, Xia Shi, Rui Ma
  • Patent number: 10415021
    Abstract: Mutated fucosidases are provided demonstrating improved properties in terms of thermal stability and transfucosidase synthetic performance compared with a wild type transfucosidase isolated from Bifidobacterium longum subsp. infantis.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: September 17, 2019
    Assignee: GLYCOM A/S
    Inventors: Elise Champion, Andreas Vogel, Sebastian Bartsch, Gyula Dekany
  • Patent number: 10400255
    Abstract: Methods employed for the discovery, enrichment, and characterization of a marine consortium of fermentative and methanogenic microorganisms developed from the solid waste digestor of a fully contained, land-based, marine recirculating aquaculture system are described. The methanogenic microbial consortium discovered is capable of reducing over 90% of marine fish waste in an aquaculture system to biomethane and carbon dioxide at saline concentrations found in marine aquaculture. Systems and methods for the treatment of marine fish waste utilizing the methanogenic marine consortium are also disclosed.
    Type: Grant
    Filed: December 14, 2016
    Date of Patent: September 3, 2019
    Inventor: Kevin R. Sowers
  • Patent number: 10400197
    Abstract: Compositions comprising lipases and biosurfactants, especially psychrophilic lipases and biosurfactants.
    Type: Grant
    Filed: August 25, 2016
    Date of Patent: September 3, 2019
    Assignee: CONOPCO INC.
    Inventors: Simone Antonio De Rose, Dietmar Andreas Lang, Jennifer Ann Littlechild-Bond, Halina Rose Novak, Sukriti Singh
  • Patent number: 10392640
    Abstract: Disclosed is a method for modifying starch to slow down the digestion rate of modified starch. The present invention utilizes two-stage GBE treatment to convert the long straight-chain starch molecules into highly branched molecules with compact structures so as to decrease the digestion rate of the starch. During the two-stage treatment, granular starch was first treated with GBE, the treated starch was gelatinized in boiling water, and the gelatinized starch was treated with GBE for a second time, leading to an enhanced effect of GBE modification. Compared with the one-staged GBE modification, the two-stage GBE modification can further increase the content of slowly digestible starch in the modified starch and thus decrease the digestion rate of starch.
    Type: Grant
    Filed: July 12, 2018
    Date of Patent: August 27, 2019
    Assignee: Jiangnan University
    Inventors: Zhaofeng Li, Zhengbiao Gu, Junyan Ren, Caiming Li, Li Cheng, Yan Hong
  • Patent number: 10392634
    Abstract: The present invention provides a method for high-efficiency production of pinoresinol by use of an H2O2 auto-scavenging enzymatic cascade. It uses eugenol as the substrate, which is relatively inexpensive and is industrially available. It uses an enzymatic cascade to remove H2O2 produced in the process of pinoresinol synthesis, thereby reducing its inhibitory effect on the enzyme activity. In addition, the present invention uses whole cells as a catalyst, which can continuously regenerate cofactors needed by the enzyme, thus eliminating the need for exogenous addition of expensive cofactors during the reaction. The yield of the present invention can reach 7.12 g/L and the conversion rate is 61.55%.
    Type: Grant
    Filed: March 28, 2017
    Date of Patent: August 27, 2019
    Assignee: Jiangnan University
    Inventors: Jingwen Zhou, Jian Chen, Yongkun Lv, Guocheng Du
  • Patent number: 10392635
    Abstract: Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
    Type: Grant
    Filed: September 5, 2018
    Date of Patent: August 27, 2019
    Assignee: Librede Inc.
    Inventors: Jason L. Poulos, Anthony N. Farnia
  • Patent number: 10370420
    Abstract: Genetically encoded, photocleavable proteins are derived from a fluorescent protein. Upon illumination, the proteins photocleave and spontaneously dissociate into two or more fragments or release one end of an internal loop.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: August 6, 2019
    Inventors: Robert Campbell, Wei Zhang, Hiofan Hoi
  • Patent number: 10358668
    Abstract: The present disclosure generally relates to biological platforms for the conversion of cellulosic biomass into fuels and chemicals. More specifically, the present disclosure relates to the conversion of cellulosic materials into sugar acids or their salts, which may then be used to produce commodity chemicals. In one aspect, the present disclosure relates to a recombinant host cell including: reduced activity of one or more polypeptides having P-glucosidase activity as compared to a corresponding wild type cell, where each of said one or more polypeptides are encoded by a gene that has at least 80% sequence identity to a gene.
    Type: Grant
    Filed: April 28, 2015
    Date of Patent: July 23, 2019
    Assignee: The Regents Of The University Of California
    Inventors: Zhiliang Fan, Amanda Hildebrand
  • Patent number: 10358635
    Abstract: The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: June 20, 2018
    Date of Patent: July 23, 2019
    Assignee: NOVOZYMES A/S
    Inventors: Padmavathi Balumuri, Rakhi Saikia, Allan Svendsen, Lone Baunsgaard
  • Patent number: 10351884
    Abstract: Process for enriching microalgal biomass, in particular of the genus Nannochloropsis, with different polyunsaturated fatty acids, primarily docosahexaenoic acid (22:6 n-3, DHA), using oils that are rich in these fatty acids. The process comprises the following steps: Preparing a solution or an emulsion of lipids+emulsifying agent (BSA), Preparing the biomass concentrate to obtain a final concentration of mg/ml or greater, Enriching the biomass concentrate by adding the lipids or the emulsion thereof to the microalgal concentrate, Allowing the mixture to rest under constant stirring for at least 24 hours under lighting conditions. The process allows for the simultaneous enrichment of microalgal biomass of the genus Nannochloropsis with eicosapentaenoic acid (20:5 n-3) (EPA) and docosahexaenoic acid (22:6 n-3) (DHA), to obtain a minimum EPA:DHA weight ratio of 10:1, with an EPA content of at least 10% of the total fatty acid content.
    Type: Grant
    Filed: October 15, 2014
    Date of Patent: July 16, 2019
    Inventors: Ana María Otero Casal, Isabel Freire Fontáns, Hugo Alexandre Milhazes Da Cunha, María Segura Fornieles, Juan Pablo Jiménez Martín
  • Patent number: 10351889
    Abstract: The present disclosure provides methods for producing bioproducts from novel genetically altered strains of Aureobasidium pullulans. Methods and materials for the construction of these strains, examination of the bioproducts and analysis and isolation of the bioproducts from genetically altered strains is provided. Genetically altered A. pullulans strains in which one or more genes encoding biosynthetic enzymes are knocked out is detailed and the benefits of using such strains described.
    Type: Grant
    Filed: July 18, 2016
    Date of Patent: July 16, 2019
    Assignee: The United States of America, as represented by The Secretary of Agriculture
    Inventors: Christopher D. Skory, Timothy D. Leathers, Neil P Price
  • Patent number: 10351842
    Abstract: Nucleic acid-guided ordered protein assembly (NOPA) arrays and methods for their generation and related applications are disclosed herein.
    Type: Grant
    Filed: May 14, 2015
    Date of Patent: July 16, 2019
    Assignee: Arizona Board of Regents on Behalf of Arizona State University
    Inventor: Joshua LaBaer
  • Patent number: 10344287
    Abstract: The present invention relates to a recombinant microorganism producing quinolinic acid, more particularly, a microorganism producing quinolinic acid and having attenuated activity or eliminated activity of a protein having a sequence of SEQ ID NO: 1 and a method of producing quinolinic acid by using the recombinant microorganism.
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: July 9, 2019
    Inventors: Jin Sook Chang, Ju Eun Kim, So Young Kim, Kwang Ho Na, Yong Uk Shin, Jae Min Lee, Jae Hee Lee, In Kyung Heo
  • Patent number: 10329537
    Abstract: The invention provides reassortant influenza strains.
    Type: Grant
    Filed: June 6, 2014
    Date of Patent: June 25, 2019
    Assignees: Seqirus UK Limited, Synthetic Genomics, Inc.
    Inventors: Peter Mason, Pirada Suphaphiphat, Raul Gomila, Philip Dormitzer
  • Patent number: 10323247
    Abstract: The invention discloses a method for improving the extracellular expression level of a foreign protein by means of phospholipase fusion expression. Four proteins, PLA2, MBP, CBD and SUMO, are used as a fusion tag to construct a fusion gene. Compared with an original protein MOH without any fusion tag, the extracellular expression level and enzymatic activity of all the four fusion proteins are increased to some degree. Among them, the fusion protein using PLA2 as the fusion tag has the highest expression level, which is 7.4 times higher than that of the original protein. Compared with other fusion tags, PLA2 has a low molecular weight and the fusion protein having PLA2 as the fusion tag has the highest expression level (up to 12 g·L?1 in a 7 L fermentation tank for high-density fermentation). It is shown that the secretory expression of a foreign protein can be effectively increased by using PLA2 as a fusion tag.
    Type: Grant
    Filed: June 1, 2018
    Date of Patent: June 18, 2019
    Assignee: Jiangnan University
    Inventors: Xiaowei Yu, Yan Xu
  • Patent number: 10323216
    Abstract: A cleaning agent for hard surfaces and methods for cleaning are provided herein. In one embodiment, the cleaning agent includes at least one first amylase, wherein the first amylase is an ?-amylase from Bacillus sp. No. 707 or a functional fragment or a variant thereof. The cleaning agent further includes at least one second amylase, wherein the second amylase is an AA560 ?-amylase from Bacillus sp. or a functional fragment or a variant thereof. In another embodiment, the method includes dispensing the cleaning agent into the interior of an automatic dishwasher while a dishwashing program is being executed, before the main washing cycle begins, or in the course of the main washing cycle.
    Type: Grant
    Filed: June 18, 2015
    Date of Patent: June 18, 2019
    Assignee: Henkel AG & Co. KGaA
    Inventors: Inga Kerstin Vockenroth, Thomas Weber, Silke Menke, Thomas Eiting, Nina Mussmann, Thorsten Bastigkeit, Noelle Wrubbel