Patents Examined by Yong D. Pak
  • Patent number: 11371034
    Abstract: Provided is a method for efficiently producing an M23A family protease. The method for producing an M23A family protease includes culturing bacteria of the genus Bacillus having a polynucleotide encoding a proprotein of the M23A family protease introduced thereinto to produce a mature form of the M23A family protease extracellularly from the bacteria of the genus Bacillus.
    Type: Grant
    Filed: January 15, 2019
    Date of Patent: June 28, 2022
    Assignee: Kao Corporation
    Inventors: Takahiro Hioki, Daichi Yamashita, Masatoshi Tohata
  • Patent number: 11352608
    Abstract: The present disclosure relates to a novel promoter and a method for producing L-amino acids using the promoter, and more specifically, to a novel polynucleotide having promoter activity, a vector and a microorganism of the genus Corynebacterium comprising the polynucleotide, a method for producing L-amino acids using the microorganism, and a fermented composition.
    Type: Grant
    Filed: March 8, 2019
    Date of Patent: June 7, 2022
    Inventors: Sun Hyoung Choi, Jin Sook Chang, Hyung Joon Kim, Byoung Hoon Yoon, Ji Yeon Lee, Yunjung Choi
  • Patent number: 11345910
    Abstract: Disclosed is a mussel adhesive protein including at least one photocaged 3,4-dihydroxyphenylalanine derivative residue including a protecting group on at least one hydroxyl residue of its catechol moiety. The photocaged 3,4-dihydroxyphenylalanine derivative residue replaces a naturally occurring amino acid and the protecting group can be cleaved from the 3,4-dihydroxyphenylalanine derivative residue by irradiation with UV light.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: May 31, 2022
    Assignee: Technische Universität Berlin
    Inventors: Matthias Hauf, Nediljko Budisa, Florian Richter, Tobias Baumann, Tobias Schneider
  • Patent number: 11345904
    Abstract: The present disclosure relates in general to therapeutic lysosomal enzyme fusion proteins useful for treating lysosomal storage diseases, liquid formulations comprising such fusion proteins and associated methods useful for treating lysosomal storage diseases in mammals.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: May 31, 2022
    Inventors: Saeed Moshashaee, Jason K. Pinkstaff, Adam Shaywitz, Natalie Ciaccio
  • Patent number: 11339379
    Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
    Type: Grant
    Filed: August 21, 2020
    Date of Patent: May 24, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Alrik Pieter Los, Rene Marcel De Jong, Remko Tsjibbe Winter, Ben Den Dulk
  • Patent number: 11339381
    Abstract: The present invention provides a polynucleotide having the nucleotide sequence as set forth in SEQ ID NO: 1 encoding 9-lipoxygenase. The present invention also provides recombinant plasmid expression vector comprising said polynucleotide. The recombinant protein 9-lipoxygenase encoded by the polynucleotide leads to production of lactones in fruits such as mangoes.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: May 24, 2022
    Assignee: Council of Scientific & Industrial Research
    Inventors: Vidya Shrikant Gupta, Ashish Balwant Deshpande, Hemangi Girish Chidley, Ashok Prabhakar Giri
  • Patent number: 11339380
    Abstract: The invention discloses a glutamate dehydrogenase mutant and an application thereof. The mutant is one of the following: a mutant of the amino acid sequence of SEQ ID NO. 1 which has a mutation at lysine at position 402 to phenylalanine or aspartic acid; a mutant which has a mutation at isoleucine at position 406 to phenylalanine or threonine; a mutant which has a mutation at threonine at position 121 and leucine at position 123; a mutant which has a mutation at alanine at position 379 and leucine at position 383. In the invention, the catalytic activity of glutamate dehydrogenase derived from Pseudomonas putida to 2-carbonyl-4-(hydroxymethylphosphonoyl)butanoic acid (PPO) is significantly improved by a molecular transformation method combining directed evolution and a semi-rational design; and the issue of low glutamate dehydrogenase activity in the process of preparing L-glufosinate by reductive amination is solved.
    Type: Grant
    Filed: November 14, 2019
    Date of Patent: May 24, 2022
    Inventors: Lirong Yang, Xinjian Yin, Jianping Wu, Gang Xu
  • Patent number: 11326151
    Abstract: This disclosure provides a genetically-modified bacterium from the genus Pseudomonas that comprises an exogenous nucleic acid encoding an enoyl-CoA reductase and an exogenous nucleic acid encoding an acyl-CoA reductase that produces medium chain length alcohols. The disclosure further provides methods for producing medium chain alcohols using such genetically-modified bacterium. This disclosure provides a renewable, bio-based production platform for valuable mcl-alcohols that have a wide range of industrial applications. Current production of mcl-alcohols typically occurs through the hydrogenation of plant oils and waxes. This process leads to issues of deforestation and is largely unsustainable. Utilizing waste lignin streams as the carbon source provides a more sustainable feedstock that can be generated from plant waste like corn stover. Along with this, the use of lignin avoids competition with food resources as traditional starch and sugar feedstocks.
    Type: Grant
    Filed: July 21, 2020
    Date of Patent: May 10, 2022
    Assignee: UT-Battelle, LLC
    Inventors: Adam M. Guss, Joshua R. Elmore, Jay D. Huenemann
  • Patent number: 11326173
    Abstract: The present invention concerns a method of producing and enantiomerically pure alpha-ionone. Further, the invention concerns a nucleic acid that comprises a sequence that encodes a lycopene-epsilon-cyclase (EC), a lycopene-epsilon-cyclase (EC), plasmids, which encode components of the alpha-ionone biosynthesis and a microorganism that contains heterologous nucleotide sequences which encode the enzymes geranylgeranyl-diphosphate-synthase, isopentenyl-diphosphate-isomerase (IPI), phytoene desaturase-dehydrogenase (crtI), phytoene synthase (crtB), lycopene-epsilon-cyclase (EC) and carotenoid-cleavage-dioxygenase (CCD1). Further, the invention concerns a method of producing highly pure epsilon-carotene.
    Type: Grant
    Filed: August 28, 2015
    Date of Patent: May 10, 2022
    Inventors: Guido Jach, Sanae Azdouffal, Katrin Schullehner, Peter Welters, Angela Goergen
  • Patent number: 11319532
    Abstract: Some aspects of this disclosure provide strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins comprise a Gam protein, a napDNAbp, and a cytidine deaminase. In some embodiments, the fusion proteins further comprise a UGI domain. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of a Gam protein, a cytidine deaminase and nucleic acid editing proteins or domains, are provided.
    Type: Grant
    Filed: August 30, 2018
    Date of Patent: May 3, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: David R. Liu, Kevin Tianmeng Zhao, Yongjoo Kim
  • Patent number: 11312947
    Abstract: Disclosed are a recombinant poly(ethylene terephthalate) hydrolase (PETase) expression vector, a recombinant mono(2-hydroxyethyl)terephthalate hydrolase (MHETase) expression vector, a strain for producing each of the recombinant PETase and MHETase containing each of the vectors, and a method for degrading a plastics using each of the recombinant PETase and MHETase expressed therefrom. When the recombinant hydrolases, that is, PETase and MHETase are used together, high enzymatic activity may be sustained for a long time to completely degrade the PET.
    Type: Grant
    Filed: December 2, 2019
    Date of Patent: April 26, 2022
    Inventor: Kyung Jin Kim
  • Patent number: 11306298
    Abstract: The present disclosure provides new RNA-guided nuclease systems and engineered nickases for making rational, direct edits to nucleic acids in live cells.
    Type: Grant
    Filed: August 31, 2021
    Date of Patent: April 19, 2022
    Assignee: Inscripta, Inc.
    Inventors: Juhan Kim, Benjamin Mijts, Aamir Mir
  • Patent number: 11299722
    Abstract: The present invention has an object of providing a modified lipase having excellent stability and reactivity at a high temperature. Provided is a modified lipase with improved reactivity and/or stability at a high temperature, the modified lipase having an amino acid sequence including one or more amino acid substitutions which are selected from the group consisting of T130C-S153C, A249P, F259Y, S282P, S283Y and S300P in the amino acid sequence represented by SEQ ID NO: 1, or an amino acid sequence having 90% or more sequence identity therewith.
    Type: Grant
    Filed: August 17, 2018
    Date of Patent: April 12, 2022
    Assignee: AMANO ENZYME INC.
    Inventors: Kazunori Yoshida, Tetsuya Takahashi, Kazuhiko Ishikawa
  • Patent number: 11299717
    Abstract: The invention relates to recombinant microorganisms and methods of producing citronellal, citronellol, citronellic acid, and/or citronellal/citronellol pathway intermediates and precursors.
    Type: Grant
    Filed: October 11, 2017
    Date of Patent: April 12, 2022
    Inventors: Thomas Oestergaard Tange, Johannes Haerle, Fanny Delegrange, Julien Denis Vivian De Block, Robert Charles Allan, Philipp Friedrich Berninger, Christophe Folly, Davide Antonio Ravasio, Ludivine Labagnere, Federico Brianza, Curt Aimé Friis Nielsen, Jørgen Hansen, Nora Weber, Samantha Jessica Capewell
  • Patent number: 11293038
    Abstract: Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
    Type: Grant
    Filed: November 11, 2019
    Date of Patent: April 5, 2022
    Inventors: Jason L. Poulos, Anthony N. Farina
  • Patent number: 11274284
    Abstract: Provided herein, in some aspects, are methods and compositions for cell-free production of ribonucleic acid.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: March 15, 2022
    Assignee: GreenLight Biosciences, Inc.
    Inventors: William Jeremy Blake, Drew S. Cunningham, Daniel MacEachran
  • Patent number: 11274286
    Abstract: The present invention provides engineered deoxyribose-phosphate aldolase polypeptides useful under industrial process conditions for the production of pharmaceutical and fine chemical compounds.
    Type: Grant
    Filed: July 2, 2019
    Date of Patent: March 15, 2022
    Assignee: Codexis, Inc.
    Inventors: Da Duan, Oscar Alvizo, Jovana Nazor, Harvinder Chagger Maniar, James Nicholas Riggins, Jonathan Vroom, Santhosh Sivaramakrishnan, Hao Yang, Anna Fryszkowska, Mark A. Huffman, Joshua N. Kolev, Iman Farasat, Agustina Rodriguez-Granillo, Deeptak Verma
  • Patent number: 11268076
    Abstract: The present invention relates to a variant of a parent lipase, which parent lipase has the amino acid sequence of SEQ ID NO: 2, wherein the variant has lipase activity, comprises a substitution at a position corresponding to position 51 and comprises a substitution or remains unaltered at a position corresponding to position 96 of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: December 8, 2015
    Date of Patent: March 8, 2022
    Assignee: Novozymes A/S
    Inventors: Christian Lundager Gylstorff, Christian Jackel, Jesper Vind, Allan Svendsen, Marco Malten, Thomas Agersten Poulsen
  • Patent number: 11248215
    Abstract: Systems and methods for the production of malonate in recombinant host cells.
    Type: Grant
    Filed: October 7, 2019
    Date of Patent: February 15, 2022
    Assignee: Lygos, Inc.
    Inventors: Jeffrey A. Dietrich, Jeffrey L. Fortman, Eric J. Steen
  • Patent number: 11248217
    Abstract: The present invention relates to engineered carbohydrate-active enzyme constructs that are useful for glycan polymers synthesis. The construct comprises a CD domain from a GH, wherein the domain is conjugated to CBM3a via a peptidic linker. The invention also relates to a method of improving glycan polymer synthesis by using engineered carbohydrate active enzymes comprising a CD domain and CBM3a.
    Type: Grant
    Filed: August 20, 2019
    Date of Patent: February 15, 2022
    Assignee: Rutgers, The State University of New Jersey
    Inventors: Shishir Chundawat, Chandra Kanth Bandi