Abstract: Provided is a method for biocatalytic synthesis of Sitagliptin and intermediates thereof, in particular, provided are compounds of Formula (I) and Formula (II), or pharmaceutically acceptable salts thereof, a polypeptide capable of catalyzing conversion of a compound of Formula (I) to a compound of Formula (II), a nucleic acid encoding the polypeptide, a vector and a cell comprising the nucleic acid. In addition, also provided are a method for producing a compound of Formula (II) and Sitagliptin by using the polypeptide and the compound of Formula (I), and a method for preparing the polypeptide.
Type:
Grant
Filed:
July 27, 2018
Date of Patent:
October 4, 2022
Assignees:
China Fortune Way Company, Nanjing Redwood Fine Chemical Co., Ltd.
Abstract: The present invention relates to a strain for producing chitinase and application thereof. The class of the strain is named Streptomyces diastaticus CS1801 and the preservation number thereof is CCTCC NO: M2018263. The Streptomyces diastaticus CS1801 of the present invention is derived from naturally fermented prawn paste. By fermentation of prawns, the enzyme activity of the chitosan is as high as 57.3 U/L and the content of chitooligosaccharides is 0.58 mol/L. The present invention provides a new method for producing chitooligosaccharides and has a good application prospect.
Abstract: The systems and methods herein include engineering a host to produce psilocybin using engineered enzymes, genetic changes, and exogenous psilocybin precursor addition (e.g., addition of L-tryptophan to a growing culture of a psilocybin producing recombinant host strain). The process occurs in genetically engineered host cell(s) that can produce psilocybin.
Type:
Grant
Filed:
November 16, 2020
Date of Patent:
September 13, 2022
Assignee:
CB THERAPEUTICS, INC.
Inventors:
Jacob Michael Vogan, Laura Flatauer Peiffer, James Lee Wade, Tyrone Jacob Yacoub, Kirsten Tang, Rachel Nadine Burnett
Abstract: The present disclosure relates to dish washing compositions comprising polypeptides having beta-glucanase activity, catalytic domains, beta-glucan binding domains and polynucleotides encoding the polypeptides, catalytic domains or beta-glucan binding domains. The present disclosure further relates to dish washing compositions comprising polypeptides exhibiting beta-glucanase activity and one or more amylases and/or one or more proteases and uses thereof in dish wash applications and dish wash processes.
Type:
Grant
Filed:
December 7, 2016
Date of Patent:
September 13, 2022
Assignee:
HENKEL AG & CO. KGAA
Inventors:
Thomas Weber, Inga Kerstin Vockenroth, Clarissa Maisey, Astrid Spitz, Lisa-Marie Schuetz, Claudia Ottow, Daniela Herbst, Iben Damager, Morten Gjermansen, Carsten Andersen, Claudia Lindner
Abstract: The present invention relates to laccase variants and uses thereof as eco-friendly biocatalysts in various industrial processes. More in particular, the invention relates to a polypeptide with laccase activity comprising an amino acid sequence that is at least 90% identical to the amino acid sequence according to SEQ ID NO: 1, wherein the polypeptide comprises at least one amino acid selected from the group consisting of Alanine, Proline, Aspartic acid, Isoleucine, Proline, Proline, Threonine and Proline at a position corresponding to of positions 253, 128, 384, 364, 292, 450, 33 and 322 in SEQ ID NO: 1 respectively.
Abstract: Genetically modified microorganisms that have the ability to convert carbon substrates into multicarbon products. Methods of making these genetically modified microorganisms and methods of using them. Vectors encoding enzymes for use in converting carbon substrates into multicarbon products.
Type:
Grant
Filed:
April 27, 2018
Date of Patent:
August 23, 2022
Assignee:
PRECIGEN, INC.
Inventors:
Jeffrey David Orth, Louis A. Clark, Lily Yuin Chao, Na My Trinh, Christopher Cheyney Farwell, Xinhua Zhao, Matthias Helmut Schmalisch, Grayson Thomas Wawrzyn, Xuezhi Li, Mark Anton Held, Kevin Lee Dietzel, James Kealey
Abstract: A biosensor for detecting nitrotoluenes. Two P. putida host populations (H-I and H-II) are engineered. H-1 undergoes fluorescence when a nitrotoluene is detected but it is also engineered to metabolize nitrotoluenes to toluene as its sole nitrogen-source. H-I is 1-ACC Deaminase inactive and is further engineered to efflux toluene and provide toluene to adjacent H-II. In H-II, ACC is the N-source and metabolizes toluene as the sole carbon and energy source available. The H-II cells are engineered to not be able to use medium fructose. The H-II population has a promoter/GFP construct with a promoter sensitive to toluene and thus they fluoresce from that first nitrotoluene metabolite i.e. toluene, produced by the H-I cells. This is achieved by making H-II cells mutants unable to transport and phosphorylate fructose i.e. PTSFRU gene knock-out.
Type:
Grant
Filed:
April 17, 2020
Date of Patent:
August 23, 2022
Assignee:
Research Foundation of the City University of New York
Abstract: The present invention relates to a method for producing trehalose, comprising the steps of mixing and reacting, in any order, (i) at least one alpha-phosphorylase capable of catalyzing the production of alpha-D-glucose 1-phosphate intermediate from a saccharide raw material, and from at least one phosphorus source; (ii) at least one trehalose phosphorylase capable of catalyzing the production of trehalose from an alpha-D-glucose 1-phosphate intermediate and a glucose substrate, wherein the trehalose phosphorylase is a trehalose phosphorylase variant with an amino acid sequence which differs from the amino acid sequence of a wild type trehalose phosphorylase in at least one amino acid position, (iii) at least one saccharide raw material which produces an alpha-D-glucose 1-phosphate intermediate and a co-product by catalytic action of the alpha-phosphorylase; and (iv) at least one phosphorus source selected from the group consisting of a phosphoric acids and an inorganic salt thereof.
Type:
Grant
Filed:
November 28, 2018
Date of Patent:
August 23, 2022
Assignees:
C-Lecta GmbH, New Matterhorn, LLC
Inventors:
Christopher David Bayer, Andreas Vogel, Marc Struhalla, Birgit Brucher
Abstract: The present disclosure relates to a construction method of a Mucor circinelloides cell factory for producing dihomo-?-linolenic acid and a fermentation technology, belonging to the field of genetic engineering. In the present disclosure, ?-linolenic acid elongase gene glelo is obtained from Mortierella alpine by cloning, the gene is ligated to an integrative plasmid pMAT1552, and transformed into a Mucor circinelloides defective strain Mu402, and the gene glelo is integrated into Mucor circinelloides genome through homologous recombination, to obtain the recombinant strain Mc-glelo, and finally, the expression of the gene glelo in Mucor circinelloides is realized. The lipid content in the recombinant strain Mc-glelo is not obviously different from that in the control strain Mc1552, however, the lipid composition changes greatly, and dihomo-?-linolenic acid appears in the lipids of the recombinant strain Mc-glelo, and the content thereof reaches 5.7% of the total fatty acids.
Type:
Grant
Filed:
December 12, 2020
Date of Patent:
August 16, 2022
Assignee:
SHANDONG UNIVERSITY OF TECHNOLOGY
Inventors:
Yuanda Song, Md. Ahsanul Kabir Khan, Junhuan Yang, Huaiyuan Zhang, Wu Yang, Qing Liu
Abstract: An object of the present invention is to provide a method for preparing ambrein, which can easily and efficiently obtain the ambrein. The object can be solved by a mutated tetraprenyl-?-curcumene cyclase wherein (1) a 4th amino acid residue of a DXDD motif, aspartic acid, is substituted with an amino acid other than aspartic acid, and (2) an amino acid adjacent to the N-terminus of a (A/S/G)RX(H/N)XXP motif is substituted with an amino acid other than tyrosine, or a 4th amino acid of the GXGX(G/A/P) motif is substituted with an amino acid other than leucine.
Abstract: An object of the present invention is to provide an enzyme preparation useful for reducing purine bodies, especially, in beer or beer-based beverages, and use thereof. Provided is a nucleosidase preparation having an activity ratio (U/U), which is guanine deaminase activity per nucleosidase activity, of 0.4 or less.
Abstract: The present invention relates to compositions, methods and use of a mixture of enzymes having DNase activity and an enzyme having hexosaminidase activity.
Type:
Grant
Filed:
June 2, 2017
Date of Patent:
August 16, 2022
Assignee:
NOVOZYMES A/S
Inventors:
Rebecca Munk Vejborg, Klaus Gori, Lilian Eva Tang Baltsen
Abstract: A nucleic acid molecule comprising a variant rpoC coding sequence is disclosed. The variant rpoC coding sequence encodes a variant RpoC which regulates copy number of a plasmid. Also disclosed are a recombinant microorganism comprising the nucleic acid molecule, a method for regulating copy number of a subject vector in the recombinant microorganism, and a method for making a target product by use of the recombinant microorganism.
Type:
Grant
Filed:
August 7, 2019
Date of Patent:
August 9, 2022
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Ji Sun Lee, Dong Eun Chang, So Young Kim
Abstract: The present invention is within the field of industrial protein production. The invention provides a novel expression system using dehydroorotate dehydrogenase (DHODH) as a selection marker in combination with leflunomide or a metabolite thereof, notably for use in mammalian cell lines. Expression vectors encoding DHODH, cell lines comprising said vectors and methods of producing recombinant proteins are also provided.
Abstract: The present invention relates to the field of agriculture biotechnology, specially relates to an amylase mutant having high specific activity and thermal stability, gene and use thereof. Said amylase mutant is obtained by performing substitution of S33A/S34E/V35H, and deletion of amino acids at the sites of 178 and 179 of the wild type amylase having amino acid sequence of SEQ ID NO:1, and having improved enzymatic activity and thermal stability than the wild type amylase.
Type:
Grant
Filed:
March 21, 2019
Date of Patent:
July 5, 2022
Assignee:
INSTITUTE OF ANIMAL SCIENCE
OF CHINESE ACADEMY
Inventors:
Bin Yao, Huiying Luo, Jin Qiu, Tao Tu, Huoqing Huang, Yuan Wang, Yaru Wang, Yingguo Bai, XiaoYun Su, Kun Meng
Abstract: Provided is a method for efficiently producing an M23A family protease. The method for producing an M23A family protease includes culturing bacteria of the genus Bacillus having a polynucleotide encoding a proprotein of the M23A family protease introduced thereinto to produce a mature form of the M23A family protease extracellularly from the bacteria of the genus Bacillus.
Abstract: The present disclosure relates to a novel promoter and a method for producing L-amino acids using the promoter, and more specifically, to a novel polynucleotide having promoter activity, a vector and a microorganism of the genus Corynebacterium comprising the polynucleotide, a method for producing L-amino acids using the microorganism, and a fermented composition.
Type:
Grant
Filed:
March 8, 2019
Date of Patent:
June 7, 2022
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Sun Hyoung Choi, Jin Sook Chang, Hyung Joon Kim, Byoung Hoon Yoon, Ji Yeon Lee, Yunjung Choi
Abstract: The present disclosure relates in general to therapeutic lysosomal enzyme fusion proteins useful for treating lysosomal storage diseases, liquid formulations comprising such fusion proteins and associated methods useful for treating lysosomal storage diseases in mammals.
Type:
Grant
Filed:
February 24, 2017
Date of Patent:
May 31, 2022
Assignee:
BIOMARIN PHARMACEUTICAL INC.
Inventors:
Saeed Moshashaee, Jason K. Pinkstaff, Adam Shaywitz, Natalie Ciaccio
Abstract: Disclosed is a mussel adhesive protein including at least one photocaged 3,4-dihydroxyphenylalanine derivative residue including a protecting group on at least one hydroxyl residue of its catechol moiety. The photocaged 3,4-dihydroxyphenylalanine derivative residue replaces a naturally occurring amino acid and the protecting group can be cleaved from the 3,4-dihydroxyphenylalanine derivative residue by irradiation with UV light.
Abstract: The present invention provides a polynucleotide having the nucleotide sequence as set forth in SEQ ID NO: 1 encoding 9-lipoxygenase. The present invention also provides recombinant plasmid expression vector comprising said polynucleotide. The recombinant protein 9-lipoxygenase encoded by the polynucleotide leads to production of lactones in fruits such as mangoes.
Type:
Grant
Filed:
March 31, 2017
Date of Patent:
May 24, 2022
Assignee:
Council of Scientific & Industrial Research