Patents Examined by Yong D. Pak
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Patent number: 11807873Abstract: The present disclosure relates to a genetically modified microbial cell that includes a first genetic modification resulting in the expression of an exogenous vanillate demethylase, such that the microbial cell is capable of metabolizing an S-lignin decomposition product and producing 2-pyrone-4,6-dicarboxylate (PDC).Type: GrantFiled: October 19, 2021Date of Patent: November 7, 2023Assignee: Alliance for Sustainable Energy, LLCInventors: Christopher W. Johnson, Allison Jean Zimont Werner, Gregg Tyler Beckham, Sandra Fabienne Notonier
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Patent number: 11802279Abstract: The disclosure provides a modified protein that is a combination of (i) an L-asparaginase and (ii) one or more (poly)peptide(s), wherein the (poly)peptide consists solely of proline and alanine amino acid residues, and methods of preparation and use thereof.Type: GrantFiled: June 1, 2021Date of Patent: October 31, 2023Assignee: Jazz Pharmaceuticals Ireland Ltd.Inventors: Lars Friedrich, Anne O'Donnell
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Patent number: 11795441Abstract: A composition includes two exogenous enzymes from animals for consumption by human beings to prevent, treat and/or alleviate veisalgia and/or symptoms associated therewith arising from or caused by consumption or spontaneous production of alcohol through a dual-enzyme based breakdown of the alcohol, wherein a first enzyme of the two exogenous enzymes is capable of converting alcohol into a first metabolite while a second enzyme thereof is capable of converting the first metabolite into a second metabolite which is excretable to systemic circulation after an oxidation reaction of the alcohol in the presence of the two exogenous enzymes and NAD+/NADH, and wherein the first enzyme to the second enzyme is in a molar ratio of 1:3-51 in the composition in order to avoid an elevation in the level of the first metabolite in the human being.Type: GrantFiled: September 19, 2022Date of Patent: October 24, 2023Assignee: ALCOLEAR LIMITEDInventors: Bing Lou Wong, Sek Lun Law
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Patent number: 11788068Abstract: Modified or mutant bacterial luciferases having improved activity, as compared to wild type or unmodified bacterial luciferases, are described. The modified or mutant bacterial luciferases display increased light production and/or slower signal decay. Employing these modified or mutant bacterial luciferases improve a luminescence reporter system assay by increasing the detection sensitivity, resulting in improved bioreporter/reporter assays. The invention further provides methods for using the modified or mutant bacterial luciferases, reporter assays using the modified or mutant bacterial luciferases, and kits and articles of manufacture.Type: GrantFiled: October 25, 2018Date of Patent: October 17, 2023Assignee: Roche Molecular Systems, Inc.Inventor: Shawn Lee Suko
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Patent number: 11788071Abstract: The present disclosure provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The disclosure also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.Type: GrantFiled: April 6, 2021Date of Patent: October 17, 2023Assignee: Codexis, Inc.Inventors: Scott J. Novick, Nikki Dellas
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Patent number: 11788076Abstract: The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.Type: GrantFiled: October 9, 2018Date of Patent: October 17, 2023Assignees: Hiroshima University, Repertoire Genesis IncorporationInventors: Tatsuo Ichinohe, Takashi Yamamoto, Tetsushi Sakuma, Yasuko Honjo, Takakazu Kawase, Takahiko Miyama, Ryuji Suzuki
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Patent number: 11781120Abstract: This disclosure generally relates to the use of microorganisms to make various functionalized polyketides through polyketoacyl-CoA thiolase-catalyzed non-decarboxylative condensation reactions instead of decarboxylative reactions catalyzed by polyketide synthases. Native or engineered polyketoacyl-CoA thiolases catalyze the non-decarboxylative Claisen condensation in an iterative manner (i.e. multiple rounds) between two either unsubstituted or functionalized ketoacyl-CoAs (and polyketoacyl-CoAs) serving as the primers and acyl-CoAs serving as the extender unit to generate (and elongate) polyketoacyl-CoAs. Before the next round of polyketoacyl-CoA thiolase reaction, the ?-keto group of the polyketide chain of polyketoacyl-CoA can be reduced and modified step-wise by 3-OH-polyketoacyl-CoA dehydrogenase or polyketoenoyl-CoA hydratase or polyketoenoyl-CoA reductase. Dehydrogenase converts the ?-keto group to ?-hydroxy group. Hydratase converts the ?-hydroxy group to ?-?-double-bond.Type: GrantFiled: July 15, 2020Date of Patent: October 10, 2023Inventor: Ramon Gonzalez
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Patent number: 11781160Abstract: The invention relates to methods for the production of methacrylates using methacrylate tolerant microorganisms.Type: GrantFiled: May 23, 2019Date of Patent: October 10, 2023Assignee: Mitsubishi Chemical UK LimitedInventors: Zoe Disley, Graham Ronald Eastham, David William Johnson, Laura Martins, Russel Menchavez, Luca Rossoni, Gill Stephens
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Patent number: 11773379Abstract: Disclosed are methods and reagents for the enzymatic measurement of short-chain fatty acids, having 3 to 6 carbon atoms, in a sample. Methods include the use of recombinant butyrate kinases from multiple species, combined various reagents that includes ATP, to detect butyric acid in different types of samples via measurement of ATP consumption. Disclosed also are the reagents themselves.Type: GrantFiled: April 2, 2021Date of Patent: October 3, 2023Assignee: SYSMEX CORPORATIONInventors: Shinichi Sakasegawa, Kenji Konishi, Yasushi Shirahase, Toshiyuki Yoshida
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Patent number: 11773383Abstract: Disclosed is a method for promoting extracellular expression of proteins in B. subtilis using cutinase, which belongs to the technical fields of genetic engineering, enzyme engineering and microbial engineering. It teaches co-expressing a cutinase mutant and a target protein in B. subtilis to promote extracellular expression of the target protein which is naturally located inside cells. The target protein includes xylose isomerase, 4,6-?-glucosyltransferase, 4-?-glucosyltransferase, trehalose synthase, branching enzyme and the like. The invention can achieve extracellular expression of intracellularly localized target protein, improve the production efficiency, reduce the production cost and simplify the subsequent extraction process.Type: GrantFiled: June 17, 2021Date of Patent: October 3, 2023Assignee: Jiangnan UniversityInventors: Jing Wu, Lingqia Su, Yan Huang
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Patent number: 11773386Abstract: The present invention relates to polypeptides having carbonic anhydrase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: January 20, 2021Date of Patent: October 3, 2023Assignee: Novozymes A/SInventors: Sonja Salmon, Martin Simon Borchert, Thomas Holberg Blicher, Wolfgang Streit, Mirjam Perner
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Patent number: 11767521Abstract: The present invention provides for a genetically modified bacterial host cell capable of producing indigoidine, wherein the host cell comprises a non-ribosomal peptide synthetase (NRPS) that converts glutamine to indigoidine, and the bacterial host cell is reduced in its expression of one or more of the sixteen indicated enzymes.Type: GrantFiled: February 19, 2021Date of Patent: September 26, 2023Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Thomas T. Eng, Deepanwita Banerjee, Aindrila Mukhopadhyay
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Patent number: 11767504Abstract: Disclosed herein are albumin compositions having defined fatty acid profiles and methods of using the same. The albumin compositions described herein are suitable for use in cell culture methods, protein stabilization methods, amongst others. The albumin compositions described herein may improve the viability of and/or promote the growth of cells (e.g., mammalian cells) when the cells are cultured in a medium containing the albumin compositions. The albumin compositions described herein may improve the stability of a biologic when the biologic is in the presence of the albumin compositions. Further provided herein are methods of formulating albumin compositions having defined fatty acid profiles as described herein.Type: GrantFiled: August 14, 2020Date of Patent: September 26, 2023Assignee: Albcura CorporationInventors: Po-Yi Huang, Meng-Tsung Hsu, Pei-Chin Chen, Yu-Feng Liang, Chen-Yu Hsieh, Jeffy Chern
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Patent number: 11753629Abstract: The invention is directed to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution and homologous recombination, a strain obtained by this method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use of the strain. In particular, the invention is directed to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution of CYP52A12 gene and homologous recombination, a strain obtained by this method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use of the strain.Type: GrantFiled: July 12, 2021Date of Patent: September 12, 2023Assignees: CATHAY BIOTECH INC., CIBT AMERICA INC.Inventors: Wenbo Liu, Min Xu, Howard Chou, Xiucai Liu
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Patent number: 11753656Abstract: The present invention provides for novel metabolic pathways to reduce or modulate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous proteins that function to import glycerol and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous proteins or enzymes is activated, upregulated, or downregulated.Type: GrantFiled: August 15, 2014Date of Patent: September 12, 2023Assignee: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLCInventors: Aaron Argyros, William R. Kenealy, Emily Stonehouse
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Patent number: 11746342Abstract: A method for producing a protein is provided. An objective protein is produced by culturing Talaromyces cellulolyticus having an objective protein-producing ability, which has been modified so that the activity of a YscB protein is reduced, in a culture medium.Type: GrantFiled: April 6, 2020Date of Patent: September 5, 2023Assignee: AJINOMOTO CO., INC.Inventors: Hiroaki Fukada, Hiroka Koguchi, Mitsunori Tokura
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Patent number: 11739311Abstract: The present disclosure relates to a gene recombinant vector of a collagenase, comprising a collagenase gene, wherein an amino acid sequence of a collagenase encoded by the collagenase gene is shown in SEQ ID NO. 1; moreover, a genetically engineered strain of the collagenase and a preparation method of the collagenase are also disclosed; and the collagenase prepared according to the invention is capable of degrading a bone collagen, and improving a yield of a low-molecular-weight bone collagen peptide.Type: GrantFiled: September 14, 2022Date of Patent: August 29, 2023Assignee: INSTITUTE OF FOOD SCIENCE AND TECHNOLOGY, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Yujie Guo, Chunhui Zhang, Xia Li, Hongru Zhang
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Patent number: 11725213Abstract: Polypeptides comprising maltose/maltotriose transporters are provided. Additionally, polynucleotides, DNA constructs, and vectors encoding a maltose/maltotriose transporter, or yeast cells harboring such polynucleotides are provided. The yeast cell may be a Saccharomyces eubayanus cell modified to increase the expression or transport activity of a maltose/maltotriose transporter at the plasma membrane of the cell. Further, methods are provided for making a fermentation product by culturing any one of the yeast cells described herein with a fermentable substrate. Finally, methods are provided to select for and isolate maltotriose-utilizing strains of Saccharomyces eubayanus.Type: GrantFiled: June 7, 2021Date of Patent: August 15, 2023Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Chris Todd Hittinger, EmilyClare Patricia Baker
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Patent number: 11718836Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.Type: GrantFiled: February 5, 2021Date of Patent: August 8, 2023Assignee: Roche Sequencing Solutions, Inc.Inventors: Aruna Ayer, Arkadiusz Bibillo, Preethi Sarvabhowman, Dhruti Vasudev Dalal, Ilya Lederman, Eileen Thai
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Patent number: 11718841Abstract: Provided is a method for improving the production amount of a tri- or higher galactooligosaccharide and the reaction rate by a method for producing a galactooligosaccharide characterized by allowing ?-galactosidase to react with a substrate in the presence of 5 to 60 mM sodium ions and 0.5 to 8 mM magnesium ions.Type: GrantFiled: December 22, 2021Date of Patent: August 8, 2023Assignee: KABUSHIKI KAISHA YAKULT HONSHAInventors: Masakazu Ikeda, Masahiko Ito