Patents Examined by Yong D. Pak
  • Patent number: 10781407
    Abstract: The present invention concerns a method for laundering a textile, the use of a polypeptide having DNase activity and a detergent composition comprising a polypeptide having deoxyribonuclease (DNase) activity.
    Type: Grant
    Filed: June 29, 2016
    Date of Patent: September 22, 2020
    Assignee: NOVOZYMES A/S
    Inventor: Klaus Gori
  • Patent number: 10781437
    Abstract: A method for one of altering and enhancing the stereospecificity of an enzyme comprising introducing a stereospecific editing domain into the enzyme.
    Type: Grant
    Filed: September 23, 2016
    Date of Patent: September 22, 2020
    Assignee: BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY
    Inventors: Eric Allen First, Charles Joseph Richardson
  • Patent number: 10781467
    Abstract: Provided are a 3-epimerase, an encoding polynucleotide therefor, a nucleic acid construct, vector, and host cell comprising the polynucleotide, a method for producing the 3-epimerase, and use of the 3-epimerase.
    Type: Grant
    Filed: March 31, 2017
    Date of Patent: September 22, 2020
    Assignees: LANGNAI BIOTECH CO., LTD, L&P FOOD INGREDIENT CO., LTD
    Inventors: Sanyong Wang, Chunrong Li, Shilei Han, Ming Yan, Miao Wei, Sheng Chen, Zhilin Zhang
  • Patent number: 10774352
    Abstract: A method for producing insoluble alpha-1,3-glucan is disclosed. Embodiments of the method comprise providing (i) oligosaccharides that comprise alpha-1,3 and alpha-1,6 glycosidic linkages, or (ii) oligosaccharides derived from a glucosyltransferase reaction; and contacting at least water, sucrose, a glucosyltransferase enzyme, and the oligosaccharides provided in the first step. Glucosyltransferase reaction compositions embodying such a method, and insoluble products thereof, are also disclosed. Yield and other product benefits can be realized when practicing the disclosed subject matter.
    Type: Grant
    Filed: May 22, 2018
    Date of Patent: September 15, 2020
    Assignee: DUPONT INDUSTRIAL BIOSCIENCES USA, LLC
    Inventors: Kevin D. Nagy, Susan Marie Hennessey, Yefim Brun, Michael Reichman
  • Patent number: 10738288
    Abstract: Provided is a mutant of the wild type phosphatidylcholine-specific phospholipase C of Bacillus cereus. The mutations involved comprise the amino acid residue at position 63 being mutated from asparagine to aspartic acid, the amino acid residue at position 131 being mutated from asparagine to serine, and the amino acid residue at position 134 being mutated from asparagine to aspartic acid, and may comprise the amino acid residue at position 56 being mutated from tyrosine to alanine, lysine, asparagine, glutamine, histidine or tryptophan, and further, may also comprise the amino acid residue at position 106 being mutated from methionine to valine. Also provided are a polynucleotide molecule encoding the mutant, a nucleic acid construct and a host cell comprising the polynucleotide molecule, a composition comprising the mutant, and the use of the mutant, the polynucleotide molecule, the nucleic acid construct and the host cell.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: August 11, 2020
    Assignee: Wilmar (Shanghai) Biotechnology Research & Development Center Co., Ltd.
    Inventors: Yaoji Xuan, Sitian Gu, Wei Wu, Sha Liu, Yueyi Bao, Qiwen Niu
  • Patent number: 10738286
    Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.
    Type: Grant
    Filed: June 19, 2018
    Date of Patent: August 11, 2020
    Assignee: Codexis, Inc.
    Inventors: Mathew G. Miller, Chinping Chng, Oscar Alvizo, Melissa Ann Mayo, James Nicholas Riggins, Xiang Yi, Jonathan S. Penfield, Gjalt W. Huisman, Jared Davis, Yasushi Saotome
  • Patent number: 10704038
    Abstract: The present invention relates to the construction of a new class of Targeted Secretion Inhibitors (TSIs), which comprise a non-cytotoxic protease, translocation peptide and a targeting moiety peptide, wherein the targeting moiety peptide has a free N-terminal domain and a free C-terminal domain; to a single-chain fusion protein precursor thereof, and to a method of activating said single-chain fusion protein precursor.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: July 7, 2020
    Assignee: IPSEN BIOINNOVATION LIMTIED
    Inventors: John Chaddock, Elaine Harper
  • Patent number: 10696990
    Abstract: The present disclosure relates to a novel polypeptide having O-phosphoserine (OPS) exporting activity, a polynucleotide encoding the polypeptide, a microorganism expressing the polypeptide, a method for producing OPS using the microorganism, and a method for producing cysteine or a derivative thereof comprising reacting the O-phosphoserine produced by the same with a sulfide, in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism expressing the same.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: June 30, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Sol Kim, Min Gyeong Kang, In Hwa Yoo, Jong Hyun Kim, Hye Won Kim
  • Patent number: 10683491
    Abstract: The present invention relates to subtilase variants suitable for use in, e.g., cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
    Type: Grant
    Filed: December 3, 2015
    Date of Patent: June 16, 2020
    Assignee: NOVOZYMES A/S
    Inventors: Miguel Duarte Guilherme Pereira Toscano, Leonardo De Maria
  • Patent number: 10676768
    Abstract: The present invention relates to an L-cysteine-producing mutant microorganism having introduced therein genes encoding enzymes which are involved in the L-cysteine metabolic pathway, and more particularly to an L-cysteine-producing mutant microorganism having introduced therein cysE, cysK and cysR, which are genes encoding enzymes which are involved in the L-cysteine metabolic pathway, and to a method of producing L-cysteine using the mutant microorganism. According to the present invention, L-cysteine can be produced with high efficiency as a result of regulating metabolic fluxes associated with the L-cysteine metabolic pathway of the mutant microorganism and regulating a system for supplying a sulfur source essential for synthesis of L-cysteine.
    Type: Grant
    Filed: December 7, 2016
    Date of Patent: June 9, 2020
    Assignee: Korea University Research and Business Foundation
    Inventors: Sung Ok Han, Young-Chul Joo, Jeong-Eun Hyeon, Seung-Kyou You
  • Patent number: 10669511
    Abstract: The present invention relates to detergent compositions comprising lipase variants. The present invention also relates to lipase variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: June 2, 2020
    Assignee: Novozymes A/S
    Inventors: Kim Borch, Jesper Vind
  • Patent number: 10669561
    Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.
    Type: Grant
    Filed: July 2, 2015
    Date of Patent: June 2, 2020
    Assignee: GENOMATICA, INC.
    Inventors: Stephanie J. Culler, Mark J. Burk, Robin E. Osterhout, Priti Pharkya
  • Patent number: 10655154
    Abstract: The present invention proposes a cellulose hydrolysis method including contacting a fermentation medium comprising paper sludge as carbon source with cellulase obtained on site from cellulase bacteria, until the mean glucose monomer number of cellulose molecules in the fermentation medium is decreased to a range between 5 and 500. The present invention further proposes a high-yield and low-cost method of biogas recovery from paper sludge.
    Type: Grant
    Filed: May 26, 2016
    Date of Patent: May 19, 2020
    Assignee: EPISOME BIYOTEKNOLOJIK ÜRÜNLER SANAYI VE TICARET ANONIM SIRKETI
    Inventors: Murat Balaban, Murat Bahadir Kilinc
  • Patent number: 10647973
    Abstract: A novel mannanase. A polypeptide having a specific amino acid sequence such as the amino acid represented by SEQ ID NO:2 exhibits a mannanase activity. Although this mannanase does not have homology with known mannanase at the amino acid level, the polypeptide has a mannanase activity as well as heat resistance.
    Type: Grant
    Filed: February 29, 2016
    Date of Patent: May 12, 2020
    Assignee: MEIJO UNIVERSITY EDUCATIONAL FOUNDATION
    Inventors: Motoyuki Shimizu, Masashi Kato
  • Patent number: 10647976
    Abstract: The expression plasmid vectors comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment, and/or a mutant. A backbone plasmid is capable of autonomous replication in a host cell. The host cell is not a P. aeruginosa cell. Transformants are transformed with expression plasmid vector. The transformants are not P. aeruginosa. Mutant host cells comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment and/or a mutant that has been integrated into the host cell chromosome. A polypeptide, a fragment and/or a mutant comprise Ldc2. A non-naturally occurring polynucleotide, and/or a mutant encodes polypeptide comprising Ldc2. Biobased cadaverine is produced using the transformants and the biobased cadaverine is prepared by the method. Polyamides are formed using the biobased cadaverine and compositions.
    Type: Grant
    Filed: June 26, 2014
    Date of Patent: May 12, 2020
    Assignees: Cathay Biotech Inc., CIBT America Inc
    Inventors: Howard Chou, Naiqiang Li, Xiucai Liu
  • Patent number: 10619172
    Abstract: Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: April 14, 2020
    Assignee: THE TRUSTEES OF DARTMOUTH COLLEGE
    Inventors: Aysenur Eminoglu, Daniel G. Olson, Lee R. Lynd
  • Patent number: 10619145
    Abstract: The present invention relates to animal feed or animal feed additives comprising polypeptides having protease activity and uses thereof. Specifically, the proteases are serine S1 proteases from Janibacter, Terracoccus and Knoellia, all belonging to the family Intrasporangiaceae of the suborder Micrococcineae. The proteases have a high activity at a broad pH-range (pH 3-7) and are thus highly active during the entire passage through the digestive tract. It also relates to the methods for producing the proteases and for using the proteases to improve animal performance and the nutritional value of animal feed.
    Type: Grant
    Filed: November 3, 2015
    Date of Patent: April 14, 2020
    Assignee: Novozymes A/S
    Inventors: Tine Hoff, Peter Rahbek Oestergaard, Katrine Fruergaard Pontoppidan
  • Patent number: 10612014
    Abstract: The present invention relates to a method for solubilizing arabinoxylan-containing material (particularly insoluble arabinoxylan-containing material), comprising admixing a xylan-containing material with a xylanase comprising a polypeptide sequence shown herein as SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15, or a variant, homolog, fragment or derivative thereof having at least 75% identity with SEQ ID No. 3 or SEQ ID No. 2 or SEQ ID No. 1 or SEQ ID No. 9 or SEQ ID No. 10 or SEQ ID No. 11 or SEQ ID No. 15; or a polypeptide sequence which comprises SEQ ID No. 3, SEQ ID No. 2, SEQ ID No. 1, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11 or SEQ ID No. 15 with a conservative substitution of at least one of the amino acids; or a xylanase which is encoded by a nucleotide sequence shown herein as SEQ ID No. 6, SEQ ID No. 5, SEQ ID No. 4, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, SEQ ID No. 16, SEQ ID No. 17 or SEQ ID No.
    Type: Grant
    Filed: December 15, 2017
    Date of Patent: April 7, 2020
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Luis Fernando Romero Millan, Susan Arent Lund, Brian Soegaard Laursen, Elijah G Kiarie, Zhenghong Zhang, Rosalyn Lau, Zheyong Yu, Floor K Kooy, Jan Hendrik A Van Tuijl, Bart C Koops, Mads Broegger Pedersen, Soeren Dalsgaard
  • Patent number: 10612061
    Abstract: The present invention provides for heterologous expression of beta-glucosidase (BGL) polypeptides encoded by Humicola grisea, Candida wickerhamii, Aspergillus aculeatus, Aspergillus oryzae, Penicillium decumbens, Chaetomium globosum, Neocallimastix frontalis, Debaryomyces hansenii, Kluyveromyces marxianus, or Phytophthora infestans in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed BGL. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: April 7, 2020
    Assignee: Lallemand Hungary Liquidity Management LLC
    Inventors: John McBride, Erin Wiswall
  • Patent number: 10612063
    Abstract: The present disclosure provides methods for producing bioproducts from novel genetically altered strains of Aureobasidium pullulans. Methods and materials for the construction of these strains, examination of the bioproducts and analysis and isolation of the bioproducts from genetically altered strains is provided. Genetically altered A. pullulans strains in which one or more genes encoding biosynthetic enzymes are knocked out is detailed and the benefits of using such strains described.
    Type: Grant
    Filed: May 8, 2019
    Date of Patent: April 7, 2020
    Assignee: The United States of America, as represented by the Secretary of Agriculture
    Inventors: Christopher D. Skory, Timothy D. Leathers, Neil P. Price