Abstract: Variants of chymosin with improved milk-clotting properties.

Abstract: The invention is directed to novel alkaline proteases.

Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.

Abstract: The present invention concerns a method for laundering a textile, the use of a polypeptide having DNase activity and a detergent composition comprising a polypeptide having deoxyribonuclease (DNase) activity.

Abstract: A method for one of altering and enhancing the stereospecificity of an enzyme comprising introducing a stereospecific editing domain into the enzyme.

Abstract: Provided are a 3-epimerase, an encoding polynucleotide therefor, a nucleic acid construct, vector, and host cell comprising the polynucleotide, a method for producing the 3-epimerase, and use of the 3-epimerase.

Abstract: A method for producing insoluble alpha-1,3-glucan is disclosed. Embodiments of the method comprise providing (i) oligosaccharides that comprise alpha-1,3 and alpha-1,6 glycosidic linkages, or (ii) oligosaccharides derived from a glucosyltransferase reaction; and contacting at least water, sucrose, a glucosyltransferase enzyme, and the oligosaccharides provided in the first step. Glucosyltransferase reaction compositions embodying such a method, and insoluble products thereof, are also disclosed. Yield and other product benefits can be realized when practicing the disclosed subject matter.

Abstract: Provided is a mutant of the wild type phosphatidylcholine-specific phospholipase C of Bacillus cereus. The mutations involved comprise the amino acid residue at position 63 being mutated from asparagine to aspartic acid, the amino acid residue at position 131 being mutated from asparagine to serine, and the amino acid residue at position 134 being mutated from asparagine to aspartic acid, and may comprise the amino acid residue at position 56 being mutated from tyrosine to alanine, lysine, asparagine, glutamine, histidine or tryptophan, and further, may also comprise the amino acid residue at position 106 being mutated from methionine to valine. Also provided are a polynucleotide molecule encoding the mutant, a nucleic acid construct and a host cell comprising the polynucleotide molecule, a composition comprising the mutant, and the use of the mutant, the polynucleotide molecule, the nucleic acid construct and the host cell.

Abstract: The present invention provides engineered RNA polymerase variants and compositions comprising these variants. The present invention further provides engineered T7 RNA polymerase variants and compositions comprising these variants. These variants have been evolved for selective incorporation of the m7G(5?)ppp(5?)m7G cap analog over GTP at the initiation of in vitro transcription. The present invention also provides methods for selective capping of RNA transcripts.

Abstract: The present invention relates to the construction of a new class of Targeted Secretion Inhibitors (TSIs), which comprise a non-cytotoxic protease, translocation peptide and a targeting moiety peptide, wherein the targeting moiety peptide has a free N-terminal domain and a free C-terminal domain; to a single-chain fusion protein precursor thereof, and to a method of activating said single-chain fusion protein precursor.

Abstract: The present disclosure relates to a novel polypeptide having O-phosphoserine (OPS) exporting activity, a polynucleotide encoding the polypeptide, a microorganism expressing the polypeptide, a method for producing OPS using the microorganism, and a method for producing cysteine or a derivative thereof comprising reacting the O-phosphoserine produced by the same with a sulfide, in the presence of O-phosphoserine sulfhydrylase (OPSS) or a microorganism expressing the same.

Abstract: The present invention relates to subtilase variants suitable for use in, e.g., cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.

Abstract: The present invention relates to an L-cysteine-producing mutant microorganism having introduced therein genes encoding enzymes which are involved in the L-cysteine metabolic pathway, and more particularly to an L-cysteine-producing mutant microorganism having introduced therein cysE, cysK and cysR, which are genes encoding enzymes which are involved in the L-cysteine metabolic pathway, and to a method of producing L-cysteine using the mutant microorganism. According to the present invention, L-cysteine can be produced with high efficiency as a result of regulating metabolic fluxes associated with the L-cysteine metabolic pathway of the mutant microorganism and regulating a system for supplying a sulfur source essential for synthesis of L-cysteine.

Abstract: The present invention relates to detergent compositions comprising lipase variants. The present invention also relates to lipase variants and polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

Abstract: The present invention proposes a cellulose hydrolysis method including contacting a fermentation medium comprising paper sludge as carbon source with cellulase obtained on site from cellulase bacteria, until the mean glucose monomer number of cellulose molecules in the fermentation medium is decreased to a range between 5 and 500. The present invention further proposes a high-yield and low-cost method of biogas recovery from paper sludge.

Abstract: A novel mannanase. A polypeptide having a specific amino acid sequence such as the amino acid represented by SEQ ID NO:2 exhibits a mannanase activity. Although this mannanase does not have homology with known mannanase at the amino acid level, the polypeptide has a mannanase activity as well as heat resistance.

Abstract: The expression plasmid vectors comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment, and/or a mutant. A backbone plasmid is capable of autonomous replication in a host cell. The host cell is not a P. aeruginosa cell. Transformants are transformed with expression plasmid vector. The transformants are not P. aeruginosa. Mutant host cells comprise a polynucleotide sequence encoding Ldc2 polypeptide, a fragment and/or a mutant that has been integrated into the host cell chromosome. A polypeptide, a fragment and/or a mutant comprise Ldc2. A non-naturally occurring polynucleotide, and/or a mutant encodes polypeptide comprising Ldc2. Biobased cadaverine is produced using the transformants and the biobased cadaverine is prepared by the method. Polyamides are formed using the biobased cadaverine and compositions.

Abstract: Disclosed are methods for engineering bacteria, for example, Thermoanaerobacterium saccharolyticum, that convert biomass to ethanol at high yield by deleting a single gene. Deletion of subunit A or subunit B of the hfs hydrogenase, but not deletion of subunit C or subunit D, results in an increase in ethanol yield.

Abstract: The present invention relates to animal feed or animal feed additives comprising polypeptides having protease activity and uses thereof. Specifically, the proteases are serine S1 proteases from Janibacter, Terracoccus and Knoellia, all belonging to the family Intrasporangiaceae of the suborder Micrococcineae. The proteases have a high activity at a broad pH-range (pH 3-7) and are thus highly active during the entire passage through the digestive tract. It also relates to the methods for producing the proteases and for using the proteases to improve animal performance and the nutritional value of animal feed.