Abstract: Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
Abstract: Described herein are polymerase variants that are exonuclease deficient. Some variants retain the strand displacement capability comparable to the wild-type or parental polymerase. Some variants have a strand displacement capability that is improved relative to the wild-type or parental polymerase. The variants may have an extension rate that is greater than the wild-type or parental polymerase. The variants may have a waiting time that is less than the wild-type or parental polymerase.
Abstract: A process for producing recombinant trypsin from prokaryote host cells in high yield and high specific activity is described. In particular, a process for producing recombinant trypsin from E. coli is described.
Abstract: Disclosed herein is a method of producing D-psicose. The method of producing D-psicose includes subjecting D-fructose to D-psicose epimerization to produce a D-psicose-containing solution, subjecting the D-psicose-containing solution to first cooling and ion purification, subjecting the purified D-psicose-containing solution to first concentration and second cooling, subjecting the D-psicose-containing solution, which has been subjected to first concentration and second cooling, to chromatography to obtain a D-fructose-containing mother liquor and a D-psicose-containing separated solution, and subjecting the D-psicose-containing separated solution to second concentration and third cooling to obtain D-psicose crystals, wherein the D-fructose-containing mother liquor produced by chromatography is reused in the D-psicose epimerization.
Type:
Grant
Filed:
April 12, 2016
Date of Patent:
February 16, 2021
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Joo Hang Lee, Min Hoe Kim, Seong Bo Kim, Seung Won Park
Abstract: The present invention relates to methods for production of 2?Fucosyllactose by a microbial system comprising a ?-1,2 fucosyltransferase (FucT2) polynucleotide and a Guanosine 5?-diphospho-?-L-fucose (GDP-L-fucose) synthesis pathway using lactose as a substrate. Furthermore, the present invention relates to compositions comprising the microbial system.
Type:
Grant
Filed:
March 6, 2018
Date of Patent:
February 2, 2021
Assignees:
BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, SEOUL NATIONAL UNIVERSITY R&DB FOUNDATION
Inventors:
Won-Heong Lee, Panchalee Pathanibul, Josh Quarterman, Michael J. Miller, Yong-Su Jin, Jin-Ho Seo
Abstract: The present invention relates to a method for solubilisation or hydrolysis of Municipal Solid Waste (MSW) with an enzyme blend and an enzyme composition for solubilization of Municipal Solid Waste (MSW), the enzyme composition comprising a cellulolytic background composition and a protease, lipase and/or beta-glucanase.
Type:
Grant
Filed:
August 27, 2015
Date of Patent:
January 12, 2021
Assignee:
Novozymes A/S
Inventors:
Henrik Bangsoe Nielsen, Lone Baekgaard, Joanna Wawrzynczyk, Hanne Soerensen, Lisa Rosgaard
Abstract: The present disclosure provides a system to produce soluble, folded, and post-translationally modified proteins. The system includes a fusion protein comprising a catalytic domain of an enzyme involved in post-translational protein modification and a targeting domain, and a substrate protein comprising a protein of interest and a sequence that interacts with the targeting domain. The present disclosure also provides polynucleotide sequences encoding fusion proteins and substrate proteins, vectors for expressing polynucleotide sequences, vectors comprising the polynucleotide sequences, and isolated cells comprising said vectors.
Abstract: The present disclosure relates to tagatose-6-phosphate phosphatase consisting of an amino acid sequence of SEQ ID NO: 1, a nucleic acid encoding the tagatose-6-phosphate phosphatase, and a transformant comprising the nucleic acid. Additionally, the present disclosure relates to a composition for producing tagatose, which comprises the tagatose-6-phosphate phosphatase of the present disclosure, and a method for producing tagatose using the tagatose-6-phosphate phosphatase of the present disclosure.
Type:
Grant
Filed:
June 30, 2017
Date of Patent:
December 15, 2020
Assignee:
CJ CHEILJEDANG CORPORATION
Inventors:
Sungjae Yang, Hyun Kug Cho, Young Mi Lee, Seong Bo Kim, Seong Jun Cho
Abstract: The invention is in the field of biotechnology and involves recombinant DNA technology. It provides means and methods for obtaining an insoluble active fusion protein comprising xylose isomerase activity. More in particular, the invention relates to a method for obtaining active insoluble xylose isomerase, comprising the expression in a host organism of a recombinant gene encoding a fusion protein comprising a xylose isomerase in combination with a PPIase, thereby obtaining the active insoluble xylose isomerase. It also provides recombinant fusion proteins comprising xylose isomerase activity as well as their use in converting xylose to xylulose and glucose to fructose.
Abstract: The present invention relates to a recombinant host cell which is capable of producing a dicarboxylic acid and which comprises a mutant malate dehydrogenase resulting in an increased production of the dicarboxylic acid. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting said recombinant host cell in a suitable fermentation medium and producing the dicarboxylic acid.
Type:
Grant
Filed:
July 11, 2017
Date of Patent:
September 29, 2020
Assignee:
DSM IP ASSETS B.V.
Inventors:
Alrik Pieter Los, Rene Marcel De Jong, Remko Tsjibbe Winter, Ben Den Dulk
Abstract: The present invention concerns a method for laundering a textile, the use of a polypeptide having DNase activity and a detergent composition comprising a polypeptide having deoxyribonuclease (DNase) activity.
Abstract: A method for one of altering and enhancing the stereospecificity of an enzyme comprising introducing a stereospecific editing domain into the enzyme.
Type:
Grant
Filed:
September 23, 2016
Date of Patent:
September 22, 2020
Assignee:
BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY
Inventors:
Eric Allen First, Charles Joseph Richardson
Abstract: Provided are a 3-epimerase, an encoding polynucleotide therefor, a nucleic acid construct, vector, and host cell comprising the polynucleotide, a method for producing the 3-epimerase, and use of the 3-epimerase.