Abstract: The present disclosure provides nucleic acid molecules encoding an engineered antigen receptor, such as a chimeric antigen receptor or exogenous T cell receptor, and an inhibitory nucleic acid molecule, such as an RNA interference molecule. The present disclosure further relates to nucleic acids, DNA constructs, vectors, pharmaceutical compositions, genetically-modified cells, and methods of treatment that utilize the nucleic acid molecules of the invention.

Abstract: The present invention encompasses genetically-modified immune cells (and populations thereof) expressing a microRNA-adapted shRNA (shRNAmiR) that reduces the expression of a target endogenous protein. Methods for reducing the expression of an endogenous protein in an immune cell are also provided wherein the method comprises introducing a shRNAmiR that targets the endogenous protein. Using shRNAmiRs for knocking down the expression of a target protein allows for stable knockdown of expression of endogenous proteins in immune cells.

Abstract: The present disclosure provides methods for preparing a population of genetically-modified immune cells. The methods include contacting a population of immune cells with lipid nanoparticles in the presence of an apolipoprotein. The lipid nanoparticles include mRNA encoding an engineered nuclease having specificity for a recognition sequence in the genome of the immune cells. The mRNA is delivered into the immune cells and the engineered nuclease is expressed, generating a cleavage site at the recognition sequence. Further provided are populations of genetic ally-modified immune cells produced according to the disclosed methods, pharmaceutical compositions containing such cells, and methods of treating diseases with the genetically-modified immune cells.

Abstract: The present invention encompasses genetically-modified immune cells (and populations thereof) expressing a microRNA-adapted shRNA (shRNAmiR) that reduces the expression of a target endogenous protein. Methods for reducing the expression of an endogenous protein in an immune cell are also provided wherein the method comprises introducing a shRNAmiR that targets the endogenous protein. Using shRNAmiRs for knocking down the expression of a target protein allows for stable knockdown of expression of endogenous proteins in immune cells.

Abstract: Methods, systems, computer-readable media, and apparatuses are presented for generating a photorealistic viewable model using augmented reality (AR). An AR scene is generated by overlaying a virtual object onto a view of a physical environment. When placed into the AR scene, the virtual object can interact with the physical environment by, for example, reflecting or taking on colors, shadows, brightness, and other attributes of the physical environment. To generate the viewable model, the virtual object is manipulated (e.g., moved or rotated) within the AR scene and a plurality of images are generated by capturing the virtual object as the virtual object is being manipulated. The viewable model can be generated based on one or more of the images and can be output in the form of an interactive presentation, for example, a spin image.

Abstract: The present invention utilizes carrier particles to present antigen peptides and proteins to the immune system in such a way as to include antigen specific tolerance. The carrier particle is designed in order to trigger an immune tolerance effect. The invention is useful for treatment of immune related disorders such as autoimmune disease, transplant rejection and allergic reactions.

Abstract: In a memory subsystem, a memory controller can put its physical interface (PHY) into a low power state when an associated memory device is in self-refresh. Instead of powering on the interface and then triggering the memory device to exit self-refresh, or instead waiting for the physical interface to be powered up prior to waking the memory device from self-refresh, the memory controller can instruct the PHY to send a self-refresh exit command to the memory device and power up the physical interface in parallel with the memory device coming out of self-refresh. The memory controller can power down a high speed clock path of the PHY and use a slower clock path to send the self-refresh exit command before powering the high speed clock path back up.

Abstract: The present disclosure provides novel co-stimulatory domains useful in genetically-modified cells to promote cell proliferation and/or promote cytokine secretion after antigen recognition. For example, disclosed herein are genetically-modified cells comprising a chimeric antigen receptor or an inducible regulatory construct incorporating the co-stimulatory domains disclosed herein. Also disclosed herein are plasmids and viral vectors comprising a nucleic acid sequence encoding the co-stimulatory domains, and methods of administering compositions comprising the novel co-stimulatory domains to subjects in order to reduce the symptoms, progression, or occurrence of disease, such as cancer.

Abstract: Aspects of the subject disclosure may include, for example, identifying a first line item having a guaranteed delivery requirement; determining whether a capacity forecast is available for the first line item and whether a forecasted capacity for the first line item is greater than a capacity threshold; responsive to the capacity forecast being available for the first line item and the forecasted capacity being greater than the capacity threshold, accessing a forecast shape curve for the first line item, wherein generating the forecast shape curve comprises normalizing the forecasted capacity based on weighting for a particular time period of a day being equal to forecasted impressions for the particular time period divided by a sum of forecasted impressions over the day; and determining whether to sell an ad space in video content to the first line item pursuant to the guaranteed delivery requirement or to a second line item pursuant to a real-time bidding process. Other embodiments are disclosed.

Abstract: The present invention encompasses genetically-modified immune cells (and populations thereof) expressing a microRNA-adapted shRNA (shRNAmiR) that reduces the expression of a target endogenous protein. Methods for reducing the expression of an endogenous protein in an immune cell are also provided wherein the method comprises introducing a shRNAmiR that targets the endogenous protein. Using shRNAmiRs for knocking down the expression of a target protein allows for stable knockdown of expression of endogenous proteins in immune cells.

Abstract: The present invention encompasses genetically-modified immune cells (and populations thereof) expressing a microRNA-adapted shRNA (shRNAmiR) that reduces the expression of a target endogenous protein. Methods for reducing the expression of an endogenous protein in an immune cell are also provided wherein the method comprises introducing a shRNAmiR that targets the endogenous protein. Using shRNAmiRs for knocking down the expression of a target protein allows for stable knockdown of expression of endogenous proteins in immune cells.

Abstract: Methods, systems, computer-readable media, and apparatuses are presented for generating a photorealistic viewable model using augmented reality (AR). An AR scene is generated by overlaying a virtual object onto a view of a physical environment. When placed into the AR scene, the virtual object can interact with the physical environment by, for example, reflecting or taking on colors, shadows, brightness, and other attributes of the physical environment. To generate the viewable model, the virtual object is manipulated (e.g., moved or rotated) within the AR scene and a plurality of images are generated by capturing the virtual object as the virtual object is being manipulated. The viewable model can be generated based on one or more of the images and can be output in the form of an interactive presentation, for example, a spin image.

Abstract: An apparatus is provided, where the apparatus includes a plurality of components; a first circuitry to generate a clock signal, and to supply the clock signal to the plurality of components; a second circuitry to estimate, for each of two or more components of the plurality of components, a corresponding duty cycle of the clock signal received at the corresponding component, wherein two or more duty cycles corresponding to the two or more components are determined; a third circuitry to determine an average of the two or more duty cycles; and a fourth circuitry to correct a duty cycle of the clock signal generated by the first circuitry, based at least in part on the average.

Abstract: The present invention encompasses genetically-modified immune cells (and populations thereof) expressing a microRNA-adapted shRNA (shRNAmiR) that reduces the expression of a target endogenous protein. Methods for reducing the expression of an endogenous protein in an immune cell are also provided wherein the method comprises introducing a shRNAmiR that targets the endogenous protein. Using shRNAmiRs for knocking down the expression of a target protein allows for stable knockdown of expression of endogenous proteins in immune cells.

Abstract: Techniques are disclosed for advantageously forecasting an inventory of a product having a particular duration (e.g., video content). The technique can include determining the particular configuration (e.g., maximum number of impressions, maximum amount of time permitted) of breaks identified within an impression log. Based on the number of breaks and the configurations of the breaks, the technique can forecast a future inventory of the product. In some implementations, the disclosed technique can identify an amount of a previous break that was not filled with video content. These forecasts can enable product sellers to better communicate with their customers, negotiate supply contracts, price their products, plan for business operations, etc.

Abstract: An apparatus is provided, where the apparatus includes a plurality of components; a first circuitry to generate a clock signal, and to supply the clock signal to the plurality of components; a second circuitry to estimate, for each of two or more components of the plurality of components, a corresponding duty cycle of the clock signal received at the corresponding component, wherein two or more duty cycles corresponding to the two or more components are determined; a third circuitry to determine an average of the two or more duty cycles; and a fourth circuitry to correct a duty cycle of the clock signal generated by the first circuitry, based at least in part on the average.

Abstract: Techniques are disclosed for advantageously forecasting an inventory of a product having a particular duration (e.g., video content). The technique can include determining the particular configuration (e.g., maximum number of impressions, maximum amount of time permitted) of breaks identified within an impression log. Based on the number of breaks and the configurations of the breaks, the technique can forecast a future inventory of the product. In some implementations, the disclosed technique can identify an amount of a previous break that was not filled with video content. These forecasts can enable product sellers to better communicate with their customers, negotiate supply contracts, price their products, plan for business operations, etc.

Abstract: Aspects of the subject disclosure may include, for example, receiving a request to forecast allocations for a new descriptor, the new descriptor differing from preexisting descriptors, each of the preexisting descriptors being associated with a subset of locations of a network of locations, each location corresponding to an electronic segment in electronic canvases, and the new descriptor being associated with a new subset of locations of the network of locations, identifying one or more affected descriptors having one or more overlapping subsets of locations of the network of locations and one or more non-overlapping subsets of locations of the network of locations, determining a forecast of allocated locations in each of the one or more affected descriptors; identifying, according to the forecast, at least a portion of allocated locations in the one or more overlapping subsets of locations that are displaceable resulting in a number of displaceable allocations, and determining, according to the number of di

Abstract: The present invention encompasses genetically-modified immune cells (and populations thereof) expressing a microRNA-adapted shRNA (shRNAmiR) that reduces the expression of a target endogenous protein. Methods for reducing the expression of an endogenous protein in an immune cell are also provided wherein the method comprises introducing a shRNAmiR that targets the endogenous protein. Using shRNAmiRs for knocking down the expression of a target protein allows for stable knockdown of expression of endogenous proteins in immune cells.

Abstract: Aspects of the subject disclosure may include, for example, receiving a request to forecast allocations for a new descriptor, the new descriptor differing from preexisting descriptors, each of the preexisting descriptors being associated with a subset of locations of a network of locations, each location corresponding to an electronic segment in electronic canvases, and the new descriptor being associated with a new subset of locations of the network of locations, identifying one or more affected descriptors having one or more overlapping subsets of locations of the network of locations and one or more non-overlapping subsets of locations of the network of locations, determining a forecast of allocated locations in each of the one or more affected descriptors; identifying, according to the forecast, at least a portion of allocated locations in the one or more overlapping subsets of locations that are displaceable resulting in a number of displaceable allocations, and determining, according to the number of di