Patents by Inventor Alfred Puhler

Alfred Puhler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9719064
    Abstract: The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type—and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene- and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.
    Type: Grant
    Filed: August 1, 2011
    Date of Patent: August 1, 2017
    Assignee: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Klaus Selber, Bernhard Weingaertner, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Patent number: 9562249
    Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AICEs from other species.
    Type: Grant
    Filed: November 12, 2015
    Date of Patent: February 7, 2017
    Assignee: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Publication number: 20160130622
    Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AICEs from other species.
    Type: Application
    Filed: November 12, 2015
    Publication date: May 12, 2016
    Inventors: Andreas KLEIN, Klaus SELBER, Hermann WEHLMANN, Winfried ROSEN, Alfred PÜHLER, Patrick SCHWIENTEK, Jörn KALINOWSKI, Udo WEHMEIER
  • Patent number: 9217154
    Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: December 22, 2015
    Assignee: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Publication number: 20140349346
    Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.
    Type: Application
    Filed: December 4, 2012
    Publication date: November 27, 2014
    Applicant: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Publication number: 20130302855
    Abstract: The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type-and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene-and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.
    Type: Application
    Filed: August 1, 2011
    Publication date: November 14, 2013
    Applicant: BAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Klaus Selber, Bernhard Weingaertner, Hermann Wehlmann, Winfried Rosen, Alfred Puhler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
  • Patent number: 8334126
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: December 18, 2012
    Assignee: Evonik Degussa GmbH
    Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jörn Kalinowski, Alfred Pühler
  • Publication number: 20120064582
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Application
    Filed: September 22, 2011
    Publication date: March 15, 2012
    Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jörn Kalinowski, Alfred Pühler
  • Patent number: 8048665
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: November 27, 2002
    Date of Patent: November 1, 2011
    Assignee: Evonik Degussa GmbH
    Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jörn Kalinowski, Alfred Pühler
  • Publication number: 20100190653
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Application
    Filed: November 27, 2002
    Publication date: July 29, 2010
    Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jorn Kalinowski, Alfred Puhler
  • Patent number: 7759056
    Abstract: The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide which encodes a polypeptide containing the amino acid sequence according to SEQ ID no. 2, b) polynucleotide which encodes a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and to a process for the fermentative production of L-amino acids, in particular L-lysine.
    Type: Grant
    Filed: December 29, 2003
    Date of Patent: July 20, 2010
    Assignee: Evonik Degussa GmbH
    Inventors: Bettina Möckel, Anke Weissenborn, Walter Pfefferle, Michael Hartmann, Jörn Kalinowski, Alfred Pühler
  • Patent number: 7262036
    Abstract: Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Grant
    Filed: May 22, 2006
    Date of Patent: August 28, 2007
    Assignee: Degussa AG
    Inventors: Bettina Hädrich, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
  • Patent number: 7132272
    Abstract: The present invention relates to an isolated polynucleotide coding for the lrp gene and to a process for the fermentative production of L-amino acids by altering the expression of this gene.
    Type: Grant
    Filed: April 30, 2002
    Date of Patent: November 7, 2006
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Jörn Kalinowski, Alfred Pühler, Bettina Möckel, Walter Pfefferle
  • Patent number: 7118904
    Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
    Type: Grant
    Filed: December 8, 2003
    Date of Patent: October 10, 2006
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Thomas Hermann, Alfred Pühler, Jörn Kalinowski, Brigitte Bathe
  • Publication number: 20060205046
    Abstract: Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Application
    Filed: May 22, 2006
    Publication date: September 14, 2006
    Inventors: Bettina Mockel, Walter Pfefferle, Achim Marx, Jorn Kalinowski, Brigitte Bathe, Alfred Puhler
  • Patent number: 7074607
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Grant
    Filed: August 14, 2003
    Date of Patent: July 11, 2006
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
  • Patent number: 6982162
    Abstract: The present invention refers to a Corynebacterium glutamicum bacterial strain which new isolated and identified secD and/or secF gene(s) is/are genetically modified in a way that secretion of the bacterial strain is enhanced, the protein- and polynucleotide sequences of these genes, a plasmid containing at least one of these genes and the use of such a bacterial strain for production of a desired substance or in a reporter system.
    Type: Grant
    Filed: May 10, 2001
    Date of Patent: January 3, 2006
    Assignee: Degussa AG
    Inventors: Stephan Berens, Jörn Kalinowski, Alfred Pühler
  • Publication number: 20050196848
    Abstract: An isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b) and d) polynucleotide containing at least 15 successive bases of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids by attenuation of the poxB gene.
    Type: Application
    Filed: April 22, 2005
    Publication date: September 8, 2005
    Inventors: Nicole Dusch, Brigitte Bathe, Jorn Kalinowski, Alfred Puhler, Bettina Mockel, Anke Weissenborn, Walter Pfefferle
  • Publication number: 20050100927
    Abstract: The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide which encodes a polypeptide containing the amino acid sequence according to SEQ ID no. 2, b) polynucleotide which encodes a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and to a process for the fermentative production of L-amino acids, in particular L-lysine.
    Type: Application
    Filed: December 29, 2003
    Publication date: May 12, 2005
    Applicant: Degussa AG
    Inventors: Bettina Mockel, Anke Weissenborn, Walter Pfefferle, Michael Hartmann, Jorn Kalinowski, Alfred Puhler
  • Publication number: 20050081267
    Abstract: A Novel deacetylase genes for use in conditional cell ablation are described. These genes are particularly useful for the production of transgenic plants with plant parts which can be destroyed upon treatment with N-acetyl-PPT.
    Type: Application
    Filed: March 12, 2002
    Publication date: April 14, 2005
    Applicant: Bayer Bioscience N.V.
    Inventors: Alfred Puhler, Inge Broer, Guido Kriete