Patents by Inventor Alfred Puhler

Alfred Puhler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 4782022
    Abstract: Isolation and characterization of a gene which activated nitrogen fixation genes of Rhizobium meliloti when that bacterium is in a symbiotic relationship with a plant is disclosed. This newly discovered gene, designated fix D, can activate the nifHD promoter. A method of making this inducible gene constitutive is presented. This is useful for making nifHD constitutive. The combination of the fixD promoter with heterologous structural genes is taught. Such combinations are useful for limiting expression of an encoded protein to rhizobia involved in a symbiotic relationship with a plant. Plasmids and methods useful in performance of this invention are also disclosed.
    Type: Grant
    Filed: May 22, 1985
    Date of Patent: November 1, 1988
    Assignee: Lubrizol Genetics, Inc.
    Inventors: Alfred Puhler, Helmut Reilaender, Gerhard Weber
  • Patent number: 4686184
    Abstract: A Tn-Mob element is provided comprising a transposon with a Mob-site inserted therein. Vectors and bacterial strains are provided containing the Tn-Mob element, as well as methods for its use. Such methods include the production of plasmid free bacterial strains.
    Type: Grant
    Filed: July 1, 1983
    Date of Patent: August 11, 1987
    Assignee: Lubrizol Genetics, Inc.
    Inventors: Alfred Puhler, Reinhard Simon
  • Patent number: 4680264
    Abstract: Vectors capable of replication in a broad host range of gram-negative bacteria are provided. The vectors are composite plasmids comprising a segment derived from an E. coli vector plasmid and a segment derived from a broad host range plasmid. The broad host range plasmid segment includes Mob-functions and DNA coding for replication functions with regions non-essential for broad host range replication or mobilizablity having been deleted. Bacterial strains containing the above plasmids as well as methods of cloning DNA employing such plasmids are also provided.
    Type: Grant
    Filed: July 1, 1983
    Date of Patent: July 14, 1987
    Assignee: Lubrizol Genetics, Inc.
    Inventors: Alfred Puhler, Reinhard Simon, Ursula Priefer
  • Patent number: 4673642
    Abstract: The plasmid p SVH 1, which is obtained from Streptomyces venezuelae DSM 40755 and which has a molecular weight of 8.4 megadaltons, a contour length of 4.1 .mu.m and a molecular size of 12.6 kilobases, and its use for the construction of a vector and for cloning foreign DNA in suitable host organisms.
    Type: Grant
    Filed: August 20, 1984
    Date of Patent: June 16, 1987
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Alfred Puhler, Wolfgang Wolleben, Michael Leineweber
  • Patent number: 4626504
    Abstract: Modified E. coli vectors are disclosed that are mobilizable to a wide range of gram-negative bacteria, but are not self-transmissible. The modified E. coli plasmids replicate only in bacterial strains of the E. coli group. Mobilizer strains of E. coli are also provided, as well as methods for employing the modified E. coli vectors for transposon mutagenesis, site-specific gene transfer, and the construction of DNA libraries.
    Type: Grant
    Filed: July 1, 1983
    Date of Patent: December 2, 1986
    Assignee: Lubrizol Genetics, Inc.
    Inventors: Alfred Puhler, Reinhard Simon
  • Patent number: 4621061
    Abstract: The new streptomyces plasmid p SG 2, having a molecular weight of 9.2 megadaltons, a contour length of 4.58 .mu.m and a molecular length of about 13.8 kilobases, is described, together with its preparation from a culture of "Streptomyces ghanaensis" ATCC 14 672.
    Type: Grant
    Filed: December 13, 1983
    Date of Patent: November 4, 1986
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Alfred Puhler, Wolfgang Wohlleben, Michael Leineweber