Patents by Inventor Alfred Puhler

Alfred Puhler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20040220394
    Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
    Type: Application
    Filed: December 8, 2003
    Publication date: November 4, 2004
    Applicant: Degussa AG
    Inventors: Bettina Mockel, Walter Pfefferle, Thomas Hermann, Alfred Puhler, Jorn Kalinowski, Brigitte Bathe
  • Patent number: 6787334
    Abstract: The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.
    Type: Grant
    Filed: October 6, 2000
    Date of Patent: September 7, 2004
    Assignee: Degussa AG
    Inventors: Frank Elischweski, Jorn Kalinowski, Alfred Puhler, Nicole Dusch, Jurgen Dohmen, Mike Farwick, Georg Thierbach
  • Patent number: 6777229
    Abstract: This invention relates to the mutually compatible plasmids pTET3 and pCRY4, isolated from the strain of Corynebacterium glutamicum deposited under DSM number 5616, wherein plasmid pTET3 is characterised by 1.1 a length of ˜27.8 kbp and the restriction map shown in FIG. 1, 1.2 a replication region comprising the nucleotide sequence shown in SEQ ID no. 1 and 1.3 an antibiotic resistance region consisting of a tetA gene imparting tetracycline resistance and an aadA gene imparting streptomycin and spectinomycin resistance, shown in SEQ ID no. 6, and plasmid pCRY4 is characterised by 1.4 a length of ˜48 kbp and the restriction map shown in FIG. 2 and 1.5 a replication region comprising the nucleotide sequence shown in SEQ ID no. 4 to composite plasmid vectors of these plasmids which are capable of autonomous replication in coryneform bacteria and to processes for the production of L-amino acids, vitamins and nucleotides using these bacteria.
    Type: Grant
    Filed: November 3, 2000
    Date of Patent: August 17, 2004
    Assignee: Degussa AG
    Inventors: Andreas Tauch, Jorn Kalinowski, Alfred Puhler, Georg Thierbach
  • Patent number: 6740742
    Abstract: The invention relates to an isolated polynucleotide from coryneform bacteria wherein the polynucleotide encodes a protein having n-succinylaminokeptopimelate transaminase activity.
    Type: Grant
    Filed: March 22, 2001
    Date of Patent: May 25, 2004
    Assignee: Degussa AG
    Inventors: Bettina Mockel, Anke Weissenborn, Walter Pfefferle, Michael Hartmann, Jörn Kalinowski, Alfred Pühler
  • Publication number: 20040063180
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of
    Type: Application
    Filed: August 14, 2003
    Publication date: April 1, 2004
    Inventors: Bettina Mockel, Walter Pfefferle, Achim Marx, Jorn Kalinowski, Brigitte Bathe, Alfred Puhler
  • Patent number: 6713289
    Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
    Type: Grant
    Filed: May 21, 2001
    Date of Patent: March 30, 2004
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Thomas Hermann, Alfred Pühler, Jörn Kalinowski, Brigitte Bathe
  • Patent number: 6670156
    Abstract: The present application is directed to a diaminopimelate epimerase from Corynebacterium glutamicum and to polynucleotides encoding this enzyme. The gene has been given the designation “dapF.
    Type: Grant
    Filed: October 27, 1999
    Date of Patent: December 30, 2003
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Brigitte Bathe, Jorn Kalinowski, Oliver Kirchner, Michael Hartmann, Alfred Pühler
  • Patent number: 6632644
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Grant
    Filed: December 8, 2000
    Date of Patent: October 14, 2003
    Assignee: DeGussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
  • Publication number: 20030148476
    Abstract: An isolated polynucleotide from coryneform bacteria containing a polynucleotide sequence selected from the group:
    Type: Application
    Filed: November 25, 2002
    Publication date: August 7, 2003
    Inventors: Lutz Wehmeier, Andreas Tauch, Alfred Puhler, Jorn Kalinowski, Bettina Mockel
  • Publication number: 20030087400
    Abstract: The invention relates to a process for the production of L-amino acids, and in particular L-lysine, in which the following steps are performed,
    Type: Application
    Filed: June 25, 2002
    Publication date: May 8, 2003
    Inventors: Bettina Mockel, Walter Pfefferle, Sven Brand, Alfred Puhler, Jorn Kalinowski, Brigitte Bathe
  • Patent number: 6555733
    Abstract: The invention relates to DNA molecules which code for deacetylases or proteins having the biological activity of a deacetylase and also transgenic plant cells which were transformed using the DNA molecules according to the invention. These molecules can be used for the production of plants having specifically destroyable parts, i.e. male- or female-sterile plants, by means of the specific expression of a deacetylase gene.
    Type: Grant
    Filed: November 30, 2000
    Date of Patent: April 29, 2003
    Assignee: Hoechst Schering AgrEvo GmbH
    Inventors: Klaus Bartsch, Guido Kriete, Inge Broer, Alfred Pühler
  • Publication number: 20030017555
    Abstract: The present invention relates to an isolated polynucleotide coding for the lrp gene and to a process for the fermentative production of L-amino acids by altering the expression of this gene.
    Type: Application
    Filed: April 30, 2002
    Publication date: January 23, 2003
    Inventors: Brigitte Bathe, Jorn Kalinowski, Alfred Puhler, Bettina Mockel, Walter Pfefferle
  • Publication number: 20020127663
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of
    Type: Application
    Filed: December 8, 2000
    Publication date: September 12, 2002
    Inventors: Bettina Mockel, Walter Pfefferle, Achim Marx, Jorn Kalinowski, Brigitte Bathe, Alfred Puhler
  • Publication number: 20020106748
    Abstract: The invention provides novel isolated polynucleotides containing a polynucleotide sequence chosen from the group
    Type: Application
    Filed: December 4, 2000
    Publication date: August 8, 2002
    Inventors: Bettina Mockel, Anke Weissenborn, Walter Pfefferle, Achim Marx, Alfred Puhler, Jorn Kalinowski, Brigitte Bathe, Nicole Dusch
  • Publication number: 20020106754
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the air gene is present in attenuated form and a vector which carries at least the air gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Application
    Filed: July 23, 2001
    Publication date: August 8, 2002
    Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jorn Kalinowski, Alfred Puhler
  • Publication number: 20020082403
    Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
    Type: Application
    Filed: May 21, 2001
    Publication date: June 27, 2002
    Inventors: Bettina Mockel, Walter Pfefferle, Thomas Hermann, Alfred Puhler, Jorn Kalinowski, Brigitte Bathe
  • Publication number: 20020055141
    Abstract: The present invention refers to a Corynebacterium glutamicum bacterial strain which new isolated and identified secD and/or secF gene(s) is/are genetically modified in a way that secretion of the bacterial strain is enhanced, the protein- and polynucleotide sequences of these genes, a plasmid containing at least one of these genes and the use of such a bacterial strain for production of a desired substance or in a reporter system.
    Type: Application
    Filed: May 10, 2001
    Publication date: May 9, 2002
    Inventors: Stephan Berens, Jorn Kalinowski, Alfred Puhler
  • Publication number: 20010052139
    Abstract: The invention relates to DNA molecules which code for deacetylases or proteins having the biological activity of a deacetylase and also transgenic plant cells which were transformed using the DNA molecules according to the invention. These molecules can be used for the production of plants having specifically destroyable parts, i.e. male- or female-sterile plants, by means of the specific expression of a deacetylase gene.
    Type: Application
    Filed: November 30, 2000
    Publication date: December 13, 2001
    Inventors: Klaus Bartsch, Guido Kriete, Inge Broer, Alfred Puhler
  • Publication number: 20010049123
    Abstract: The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of
    Type: Application
    Filed: March 22, 2001
    Publication date: December 6, 2001
    Inventors: Bettina Mockel, Anke Weissenborn, Walter Pfefferle, Michael Hartmann, Jorn Kalinowski, Alfred Puhler
  • Patent number: 6184007
    Abstract: The invention is a method for the production of D-pantothenic acid by the fermentation of microorganisms in which at least the panD gene is enhanced (overexpressed), optionally in combination with the panB and/or panC gene, to provide for the enrichment of the pantothenic acid in the medium or the cells of the microorganisms.
    Type: Grant
    Filed: May 26, 1999
    Date of Patent: February 6, 2001
    Assignee: Degussa-Huls Aktiengesellschaft
    Inventors: Nicole Dusch, Jörn Kalinowski, Alfred Puhler