Abstract: Nucleic acids encoding Parvoviral Rep proteins with a mutated nuclear localization signal (NLS) are provided. Also provided is a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein with a mutated zinc finger domain and a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein comprising an amino acid mutation at position 43, 57, 79, 97, 120, 179, 305, 484, 493 or 571 with reference to SEQ ID NO: 2. Nucleic acid constructs and cells, such as insect cells, comprising the nucleic acids are provided as well as a method for producing a recombinant Parvoviral virion using the nucleic acids.

Abstract: The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.

Abstract: The present invention provides a method for enhanced production of a gene product of interest in a cell, using the AAV replication machinery. The present invention further relates to a cell for use in the method of the invention and a non-human transgenic animal or a transgenic plant comprising a cell of the invention.

Abstract: The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.

Abstract: The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.

Abstract: The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors.

Abstract: The present invention relates to the improved production of recombinant parvoviral virions in insect cells. In particular, the invention relates to an improved process for the production of recombinant parvoviral virions in insect cells, wherein the full/empty parvoviral virion ratio is increased. The invention also relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral Rep proteins that increase the productivity of parvoviral vectors.

Abstract: A method for providing a search result includes receiving a user query and determining, in response to receiving the user query, a set of segment candidates based on the user query and an indexing structure. The indexing structure is associated with at least one segment constraint. The method further includes ranking the set of segment candidates and providing a result associated with the set of segment candidates. Another method includes ranking each segment within the set of segment candidates based on a set of prioritized features. Another method has the at least one segment constraint including at least one of a critical keyword and an exclusionary keyword. Another method further includes excluding a segment based on the at least one segment constraint comprising the exclusionary keyword.

Abstract: The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors.

Abstract: Loaning in a gaming environment may be managed by determining a loan value associated with a first player account, determining a transfer of the loan value from the first player account to a second player account, and determining a wager of the loan value from the second player account in the primary game. A loan win amount attributable to a winning outcome of the primary game when the loan value is used may be determined, and respective shares of the loan win amount may be allocated to the first and second player accounts.

Abstract: A system and method for providing a virtual customized wagering game include providing a customization interface to a first player to allow the first player to customize a virtual wagering game to create a customized virtual wagering game. The customized virtual wagering game created by the first player is stored and is provided to one or more other players alone or as part of a casino created by the first player. The one or more other players are able to play the customized virtual wagering game, and in an embodiment such play may confer a benefit such as an amount of virtual currency or a customization feature on the first player.

Abstract: Gifting in a gaming environment may be managed by determining a transfer of a gift value from a first player account to a second player account and determining a wager of the gift value from the second player account in a wagering game. A gift win amount attributable to a winning outcome of the wagering game when the gift value is used may be determined, and respective shares of the gift win amount may be allocated to the first and second player accounts.

Abstract: Existing and/or new registrants for a wagering game may be selected as qualified participants in a referral award game. The referral award game may include determining a referral award game trigger for initiating the referral award game. A draw may be conducted during which a referral award is randomly assigned to each qualified participant. The referral award may include a value component usable in at least one of the wagering game or a secondary game. Each of the wagering game, referral award game, and secondary game may be conducted in a casino game format or social game format. Information regarding the referral award may be communicated to each qualified participant.

Abstract: A binding cover having a transparent overlay cover disposed over and around the spine of the binding is configured to provide a label pocket on the spine portion of the binding cover wherein a label may be inserted easily without tools or rigid label material. In several embodiments the label pocket provides a fixed label space. In another embodiment the label pocket opens along the spine when the binding cover is opened, and returns to a closed position when closed, thereby retaining a label inserted therein. In another embodiment a method for forming the label pocket is provided.

Abstract: The present invention relates to the improved production of recombinant parvoviral virions in insect cells. In particular, the invention relates to an improved process for the production of recombinant parvoviral virions in insect cells, wherein the full/empty parvoviral virion ratio is increased. The invention also relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral Rep proteins that increase the productivity of parvoviral vectors.

Abstract: The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.

Abstract: The present invention relates nucleic acid constructs for the production of recombinant parvoviral (e.g. adeno-associated viral) vectors in insect cells, to insect cells comprising such constructs and to methods wherein the cells are used to produce recombinant parvoviral virions. The insect cells preferably comprise a first nucleotide sequence encoding the parvoviral rep proteins whereby the initiation codon for translation of the parvoviral Rep78 protein is a suboptimal initiation codon that effects partial exon skipping upon expression in insect cells. The insect cell further comprises a second nucleotide sequence comprising at least one parvoviral (AAV) inverted terminal repeat (ITR) nucleotide sequence and a third nucleotide sequence comprising a sequences coding for the parvoviral capsid proteins.

Abstract: The invention relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein a nuclear localization signal (NLS) in said Parvoviral Rep protein is mutated as compared with a corresponding wild type sequence. The invention also relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein the zinc finger domain in said Parvoviral Rep protein is mutated as compared with a corresponding wild type sequence. Further, the invention relates to a nucleic acid comprising a nucleotide sequence encoding a Parvoviral Rep protein, wherein an amino acid at position 43, 57, 79, 97, 120, 179, 305, 484, 493 or 571 of the said Parvoviral Rep protein is mutated in comparison to a corresponding wild type sequence, said amino acid position being defined with reference to SEQ ID NO: 2.

Abstract: A protective case for a portable electronic device combines a first side panel, a second, transparent side panel opposite the first side panel, and a peripheral side panel that is connected to both the first and second side panels. The second, transparent side panel is formed of first and second transparent layers that are bonded together to form a panel having a plurality of uniform, trapped-gas cells, each one fully sealed from all other cells. The transparent side panel enables full visibility of the contents of the case while providing cushioning for the contents. The first side panel also provides cushioning.

Abstract: A binding cover having a transparent overlay cover disposed over and around the spine of the binding is configured to provide a label pocket on the spine portion of the binding cover wherein a label may be inserted easily without tools or rigid label material. In several embodiments the label pocket provides a fixed label space. In another embodiment the label pocket opens along the spine when the binding cover is opened, and returns to a closed position when closed, thereby retaining a label inserted therein. In another embodiment a method for forming the label pocket is provided.