Patents by Inventor Arjang Hassibi
Arjang Hassibi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11833503Abstract: The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.Type: GrantFiled: December 7, 2021Date of Patent: December 5, 2023Assignee: InSilixa, Inc.Inventors: Andrea Cuppoletti, Arjang Hassibi, Lei Pei, Yang Liu, Kshama Jirage, Arun Manickam
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Publication number: 20230366015Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.Type: ApplicationFiled: December 12, 2022Publication date: November 16, 2023Applicant: California Institute of TechnologyInventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
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Publication number: 20230333039Abstract: A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.Type: ApplicationFiled: July 29, 2022Publication date: October 19, 2023Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
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Publication number: 20230173483Abstract: The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.Type: ApplicationFiled: December 7, 2021Publication date: June 8, 2023Inventors: Andrea Cuppoletti, Arjang Hassibi, Lei Pei, Yang Liu, Kshama Jirage, Arun Manickam
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Publication number: 20230141046Abstract: A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur.Type: ApplicationFiled: January 11, 2023Publication date: May 11, 2023Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
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Patent number: 11579111Abstract: A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur.Type: GrantFiled: April 24, 2018Date of Patent: February 14, 2023Assignee: Board of Regents, The University of Texas SystemInventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
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Patent number: 11560588Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.Type: GrantFiled: May 7, 2018Date of Patent: January 24, 2023Assignee: California Institute of TechnologyInventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
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Publication number: 20230016567Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample.Type: ApplicationFiled: September 19, 2022Publication date: January 19, 2023Applicant: California Institute of TechnologyInventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
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Patent number: 11525156Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.Type: GrantFiled: May 7, 2018Date of Patent: December 13, 2022Assignee: California Institute of TechnologyInventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
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Publication number: 20220373495Abstract: A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.Type: ApplicationFiled: December 21, 2021Publication date: November 24, 2022Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
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Patent number: 11485997Abstract: The present disclosure provides methods, devices and systems for detecting a presence of a nucleic acid molecule having a nucleic acid sequence. Detection of cyclic single base extension can be used to detect a nucleic acid molecule hybridized to a probe and detect a presence of a nucleic acid. The methods disclosed herein can detect a nucleic acid molecule present in a nucleic acid sample at low concentrations and in the presence of background nucleic acids having high sequence similarity.Type: GrantFiled: August 13, 2018Date of Patent: November 1, 2022Assignee: INSILIXA, INC.Inventor: Arjang Hassibi
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Patent number: 11447816Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample.Type: GrantFiled: August 29, 2016Date of Patent: September 20, 2022Assignee: California Institute of TechnologyInventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
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Publication number: 20220282320Abstract: The present disclosure provides methods, devices and systems that enable simultaneous multiplexing amplification reaction and real-time detection in a single reaction chamber.Type: ApplicationFiled: November 19, 2021Publication date: September 8, 2022Inventors: Arjang Hassibi, Robert G. Kuimelis, Lei Pei, Kirsten A. Johnson, Jessica C. Ebert, Arun Manickam, Tran T. Van
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Publication number: 20220195506Abstract: This present disclosure provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The present disclosure also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.Type: ApplicationFiled: July 22, 2021Publication date: June 23, 2022Applicant: California Institute of TechnologyInventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo, Jose Luis Riechmann
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Patent number: 11360029Abstract: The present disclosure provides methods, apparatus and systems for time-gated fluorescent-based detection. Time-based fluorescence analysis can be used in certain biochemical assays by measuring the emitted photon flux from fluorophores after an individual excitation pulse.Type: GrantFiled: April 6, 2020Date of Patent: June 14, 2022Assignee: INSILIXA, INC.Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh, Robert G. Kuimelis
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Publication number: 20220099613Abstract: A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.Type: ApplicationFiled: February 9, 2021Publication date: March 31, 2022Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
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Patent number: 11098345Abstract: This present disclosure provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The present disclosure also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.Type: GrantFiled: April 10, 2019Date of Patent: August 24, 2021Assignee: California Institute of TechnologyInventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo, Jose Luis Riechmann
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Publication number: 20210156813Abstract: A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.Type: ApplicationFiled: June 29, 2020Publication date: May 27, 2021Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
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Patent number: 11001881Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.Type: GrantFiled: August 29, 2017Date of Patent: May 11, 2021Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGYInventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
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Publication number: 20200292457Abstract: The present disclosure provides methods, apparatus and systems for time-gated fluorescent-based detection. Time-based fluorescence analysis can be used in certain biochemical assays by measuring the emitted photon flux from fluorophores after an individual excitation pulse.Type: ApplicationFiled: April 6, 2020Publication date: September 17, 2020Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh, Robert G. Kuimelis