Patents by Inventor Arjang Hassibi

Arjang Hassibi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11447816
    Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample.
    Type: Grant
    Filed: August 29, 2016
    Date of Patent: September 20, 2022
    Assignee: California Institute of Technology
    Inventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
  • Publication number: 20220282320
    Abstract: The present disclosure provides methods, devices and systems that enable simultaneous multiplexing amplification reaction and real-time detection in a single reaction chamber.
    Type: Application
    Filed: November 19, 2021
    Publication date: September 8, 2022
    Inventors: Arjang Hassibi, Robert G. Kuimelis, Lei Pei, Kirsten A. Johnson, Jessica C. Ebert, Arun Manickam, Tran T. Van
  • Publication number: 20220195506
    Abstract: This present disclosure provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The present disclosure also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Application
    Filed: July 22, 2021
    Publication date: June 23, 2022
    Applicant: California Institute of Technology
    Inventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo, Jose Luis Riechmann
  • Patent number: 11360029
    Abstract: The present disclosure provides methods, apparatus and systems for time-gated fluorescent-based detection. Time-based fluorescence analysis can be used in certain biochemical assays by measuring the emitted photon flux from fluorophores after an individual excitation pulse.
    Type: Grant
    Filed: April 6, 2020
    Date of Patent: June 14, 2022
    Assignee: INSILIXA, INC.
    Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh, Robert G. Kuimelis
  • Publication number: 20220099613
    Abstract: A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.
    Type: Application
    Filed: February 9, 2021
    Publication date: March 31, 2022
    Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
  • Patent number: 11098345
    Abstract: This present disclosure provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The present disclosure also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Grant
    Filed: April 10, 2019
    Date of Patent: August 24, 2021
    Assignee: California Institute of Technology
    Inventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo, Jose Luis Riechmann
  • Publication number: 20210156813
    Abstract: A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.
    Type: Application
    Filed: June 29, 2020
    Publication date: May 27, 2021
    Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
  • Patent number: 11001881
    Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Grant
    Filed: August 29, 2017
    Date of Patent: May 11, 2021
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
  • Publication number: 20200292457
    Abstract: The present disclosure provides methods, apparatus and systems for time-gated fluorescent-based detection. Time-based fluorescence analysis can be used in certain biochemical assays by measuring the emitted photon flux from fluorophores after an individual excitation pulse.
    Type: Application
    Filed: April 6, 2020
    Publication date: September 17, 2020
    Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh, Robert G. Kuimelis
  • Patent number: 10739293
    Abstract: A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.
    Type: Grant
    Filed: April 12, 2016
    Date of Patent: August 11, 2020
    Assignee: Board of Regents, The University of Texas Systems
    Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
  • Patent number: 10501778
    Abstract: The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: December 10, 2019
    Assignee: INSILIXA, INC.
    Inventors: Arjang Hassibi, Kshama Jirage, Arun Manickam, Kaveh Milaninia
  • Publication number: 20190323070
    Abstract: This present disclosure provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The present disclosure also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Application
    Filed: April 10, 2019
    Publication date: October 24, 2019
    Inventors: Arjang HASSIBI, Babak HASSIBI, Haris VIKALO, Jose Luis RIECHMANN
  • Publication number: 20190062819
    Abstract: The present disclosure provides methods, devices and systems for detecting a presence of a nucleic acid molecule having a nucleic acid sequence. Detection of cyclic single base extension can be used to detect a nucleic acid molecule hybridized to a probe and detect a presence of a nucleic acid. The methods disclosed herein can detect a nucleic acid molecule present in a nucleic acid sample at low concentrations and in the presence of background nucleic acids having high sequence similarity.
    Type: Application
    Filed: August 13, 2018
    Publication date: February 28, 2019
    Inventor: Arjang Hassibi
  • Patent number: 10174367
    Abstract: The present disclosure provides methods, devices and systems that enable simultaneous multiplexing amplification reaction and real-time detection in a single reaction chamber.
    Type: Grant
    Filed: October 12, 2016
    Date of Patent: January 8, 2019
    Assignee: INSILIXA, INC.
    Inventors: Arjang Hassibi, Kshama Jirage, Arun Manickam, Rituraj Singh
  • Publication number: 20180335399
    Abstract: A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur.
    Type: Application
    Filed: April 24, 2018
    Publication date: November 22, 2018
    Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
  • Patent number: 10106839
    Abstract: A biosensor array, system and method for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Grant
    Filed: April 1, 2013
    Date of Patent: October 23, 2018
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventor: Arjang Hassibi
  • Publication number: 20180251828
    Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Application
    Filed: May 7, 2018
    Publication date: September 6, 2018
    Inventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
  • Publication number: 20180251829
    Abstract: This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.
    Type: Application
    Filed: May 7, 2018
    Publication date: September 6, 2018
    Inventors: Arjang Hassibi, Babak Hassibi, Haris Vikalo
  • Patent number: 9983163
    Abstract: A biosensor pixel for measuring current that flows through the electrode surface in response to electrochemical interactions and a biosensor array architecture that includes such biosensor pixels. The biosensor pixel includes an electrode transducer configured to measure a current generated by electrochemical interactions occurring at a recognition layer placed directly on top of it in response to an electrical voltage placed across an electrode transducer-electrolyte interface. The biosensor pixel further includes a trans-impedance amplifier connected to the electrode transducer, where the trans-impedance amplifier is configured to convert the current into a voltage signal as the electrochemical interactions occur.
    Type: Grant
    Filed: April 30, 2013
    Date of Patent: May 29, 2018
    Assignee: Board of Regents, The University of Texas System
    Inventors: Arjang Hassibi, Arun Manickam, Rituraj Singh
  • Publication number: 20180023129
    Abstract: The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.
    Type: Application
    Filed: June 13, 2017
    Publication date: January 25, 2018
    Inventors: Arjang HASSIBI, Kshama JIRAGE, Arun MANICKAM, Kaveh MILANINIA