Patents by Inventor Ashutosh Chilkoti

Ashutosh Chilkoti has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • POLYMER CONJUGATES HAVING REDUCED ANTIGENICITY AND METHODS OF USING THE SAME
    Publication number: 20170239363
    Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.
    Type: Application
    Filed: December 21, 2016
    Publication date: August 24, 2017
    Inventors: Ashutosh Chilkoti, Yizhi Qi, Michael S. Hershfield, Nancy J. Ganson
  • POLYMER CONJUGATES HAVING REDUCED ANTIGENICITY AND METHODS OF USING THE SAME
    Publication number: 20170233714
    Abstract: Disclosed herein are compositions and methods for reducing the antigenicity of molecules, wherein the molecule comprises a uricase. The antigenicity of a molecule may be reduced or eliminated by conjugating at least one branched polymer to the molecule to form a molecule-polymer conjugate. The branched polymer may include a backbone and a plurality of side chains, each side chain covalently attached to the backbone.
    Type: Application
    Filed: December 21, 2016
    Publication date: August 17, 2017
    Inventors: Ashutosh Chilkoti, Yizhi Qi, Michael S. Hershfield, Nancy J. Ganson
  • PROGRAMMABLE LIQUID, GEL AND BIOHYBRID COMPARTMENTS AND METHODS OF USE
    Publication number: 20170216808
    Abstract: Nano- to microscale liquid coacervate particles are provided. The liquid coacervate particles are produced by a process including stimulating a population of liquid droplets containing one or a mixture of components to induce a phase separation point of a first component, and maintaining stimulation at the phase separation point to form a coacervate domain of the first component within each of the droplets to form the liquid coacervate particles. The self-assembled nano, meso, micro and macro liquid coacervate particles and related coated substrates can have utility in drug delivery, bioanalytical systems, controlled cell culture, tissue engineering, biomanufacturing and drug discovery.
    Type: Application
    Filed: April 14, 2017
    Publication date: August 3, 2017
    Inventors: Gabriel P. Lopez, Joseph R. Simon, Nick J. Carroll, Ashutosh Chilkoti
  • DETECTION DEVICES AND RELATED METHODS OF USE
    Publication number: 20170108496
    Abstract: Disclosed are devices and methods for detecting analytes from a sample.
    Type: Application
    Filed: October 27, 2016
    Publication date: April 20, 2017
    Inventors: Ashutosh CHILKOTI, Angus HUCKNALL
  • Enzyme-catalyzed synthesis of site-specific and stoichiometric biomolecule-polymer conjugates
    Patent number: 9592303
    Abstract: Methods for producing polypeptide-polymer conjugates include attachment of an initiator agent to a polypeptide specifically at the C-terminus of the polypeptide using a sortase enzyme and in situ polymerization of a polymer from the C-terminus. The polypeptide-polymer conjugates may have desirable pharmacological properties and may be used therapeutically.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: March 14, 2017
    Assignee: Duke University
    Inventors: Ashutosh Chilkoti, Yizhi Qi
  • DIRECT DETECTION OF RNA BY SURFACE INITIATED ENZYMATIC POLYMERIZATION
    Publication number: 20170058329
    Abstract: Disclosed herein are methods of detecting a target RNA, methods of diagnosing an individual with a disease or condition when a target RNA associated with the disease or condition is detected, and methods of conveying via a communication medium data from the detection of a target RNA.
    Type: Application
    Filed: November 25, 2014
    Publication date: March 2, 2017
    Inventors: Ashutosh CHILKOTI, Srinath RANGARAJAN, Miriam AMIRAM, Angus HUCKNALL
  • SYSTEMS AND DEVICES FOR PROTEASE DETECTION BASED ON ENGINEERED POLYMERS AND BIOPOLYMERS AND METHODS OF USE
    Publication number: 20160348147
    Abstract: The presently disclosed subject matter relates to modular peptide-substrate protease assays, in particular low cost and reliable methodology for measurement of protease and other enzyme activity that may be detected by optical turbidimetry or visual observation. The presently disclosed subject matter also relates to methods of using the disclosed assays within methods of detecting and monitoring diseases, methods of drug discovery, as well as in detection devices and systems.
    Type: Application
    Filed: February 4, 2015
    Publication date: December 1, 2016
    Inventors: Gabriel P. Lopez, Ashutosh Chilkoti, Ali Ghoorchian, Felipe Garcia
  • NON-FOULING POLYMERIC SURFACE MODIFICATION AND SIGNAL AMPLIFICATION METHOD FOR BIOMOLECULAR DETECTION
    Publication number: 20160333392
    Abstract: An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described.
    Type: Application
    Filed: July 25, 2016
    Publication date: November 17, 2016
    Inventor: Ashutosh Chilkoti
  • Non-fouling polymeric surface modification and signal amplification method for biomolecular detection
    Patent number: 9493823
    Abstract: An article such as a biosensor having a nonfouling surface thereon is described. The article comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; (c) a polymer layer comprising brush molecules formed on the linking layer; and (d) optionally but preferably, a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) coupled to the brush molecules. The polymer layer is preferably formed by the process of surface-initiated polymerization (SIP) of monomeric units thereon. Preferably, each of the monomeric units comprises a monomer (for example, a vinyl monomer) core group having at least one protein-resistant head group coupled thereto, to thereby form the brush molecule on the surface portion. Methods of using the articles are also described.
    Type: Grant
    Filed: January 3, 2013
    Date of Patent: November 15, 2016
    Assignee: Duke University
    Inventor: Ashutosh Chilkoti
  • Detection devices and related methods of use
    Patent number: 9482664
    Abstract: Disclosed are devices and methods for detecting analytes from a sample.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: November 1, 2016
    Assignee: Sentilus Holdco LLC
    Inventors: Ashutosh Chilkoti, Angus Hucknall
  • Therapeutic agents comprising fusions of insulin and elastic peptides
    Patent number: 9458218
    Abstract: The present invention provides therapeutic agents and compositions comprising elastic peptides and therapeutic proteins. Such peptides exhibit a flexible, extended conformation. In some embodiments, the therapeutic protein is a GLP-1 receptor agonist (e.g., GLP-1, exendin), insulin, or Factor VII/VIIa, including functional analogs. The present invention further provides encoding polynucleotides, as well as methods of making and using the therapeutic agents. The therapeutic agents have improvements in relation to their use as therapeutics, including, inter alia, one or more of half-life, clearance and/or persistance in the body, solubility, and bioavailability.
    Type: Grant
    Filed: March 4, 2014
    Date of Patent: October 4, 2016
    Assignee: DUKE UNIVERSITY
    Inventor: Ashutosh Chilkoti
  • HYDROGELS FORMED FROM POLYPEPTIDE MICELLES AND METHODS OF USE THEREOF
    Publication number: 20160271262
    Abstract: The presently disclosed subject matter is directed to compositions, methods, and systems (e.g., platforms) comprising the same, the systems comprising, consisting of, or consisting essentially of micro- and macro-hydrogels that are formed from polypeptide micelles. The systems have enhanced mechanical properties that can be useful in a wide variety of applications, can be used for controlled release of drug-loaded micelles, and can be designed to reversibly assemble and disassemble on demand.
    Type: Application
    Filed: March 18, 2016
    Publication date: September 22, 2016
    Inventors: Gabriel P. Lopez, Ashutosh Chilkoti, Ali Ghoorchian, Joseph R. Simon
  • Tunable Nonfouling Surface Of Oligoethylene Glycol
    Publication number: 20160222218
    Abstract: An article having a nonfouling surface thereon is comprises: (a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer, preferably by the process of surface-initiated polymerization of monomeric units thereon, with each of the monomeric units comprising a monomer core group having at least one protein-resistant head group coupled thereto, to thereby form a brush molecule on the surface portion. The brush molecule comprising a stem formed from the polymerization of the monomer core groups, and a plurality of branches formed from the hydrophilic head group projecting from the stem. Methods of making and using such articles, are also described.
    Type: Application
    Filed: April 7, 2016
    Publication date: August 4, 2016
    Inventors: Ashutosh CHILKOTI, Hongwei MA
  • THERAPEUTIC AGENTS COMPRISING ELASTIN-LIKE PEPTIDES
    Publication number: 20160120952
    Abstract: The present invention provides therapeutic agents and compositions comprising elastin-like peptides (ELPs) and therapeutic proteins. In some embodiments, the therapeutic protein is a GLP-1 receptor agonist, insulin, or Factor VII/VIIa, including functional analogs. The present invention further provides encoding polynucleotides, as well as methods of making and using the therapeutic agents. The therapeutic agents have improvements in relation to their use as therapeutics, including, inter alia, one or more of half-life, clearance and/or persistance in the body, solubility, and bioavailability.
    Type: Application
    Filed: October 21, 2015
    Publication date: May 5, 2016
    Inventor: Ashutosh CHILKOTI
  • ENZYME-CATALYZED SYNTHESIS OF SITE-SPECIFIC AND STOICHIOMETRIC BIOMOLECULE-POLYMER CONJUGATES
    Publication number: 20160122451
    Abstract: Methods for producing polypeptide-polymer conjugates include attachment of an initiator agent to a polypeptide specifically at the C-terminus of the polypeptide using a sortase enzyme and in situ polymerization of a polymer from the C-terminus. The polypeptide-polymer conjugates may have desirable pharmacological properties and may be used therapeutically.
    Type: Application
    Filed: May 30, 2014
    Publication date: May 5, 2016
    Inventors: Ashutosh Chilkoti, Yizhi Qi
  • Therapeutic agents comprising fusions of growth hormone and elastic peptides
    Patent number: 9328154
    Abstract: The present invention provides therapeutic agents and compositions comprising elastic peptides and therapeutic proteins. Such peptides exhibit a flexible, extended conformation. In some embodiments, the therapeutic protein is a GLP-1 receptor agonist (e.g., GLP-1, exendin), insulin, or Factor VII/VIIa, including functional analogs. The present invention further provides encoding polynucleotides, as well as methods of making and using the therapeutic agents. The therapeutic agents have improvements in relation to their use as therapeutics, including, inter alia, one or more of half-life, clearance and/or persistance in the body, solubility, and bioavailability.
    Type: Grant
    Filed: April 16, 2014
    Date of Patent: May 3, 2016
    Assignee: DUKE UNIVERSITY
    Inventor: Ashutosh Chilkoti
  • METHODS AND COMPOSITIONS FOR DELIVERING ACTIVE AGENTS WITH ENHANCED PHARMACOLOGICAL PROPERTIES
    Publication number: 20160114053
    Abstract: Provided herein are methods of enhancing in vivo efficacy of an active agent, comprising: administering to a subject an active agent that is coupled to a bioelastic polymer or elastin-like peptide, wherein the in vivo efficacy of the active agent is enhanced as compared to the same active agent when administered to the subject not coupled to (or not associated with) a bioelastic polymer or ELP.
    Type: Application
    Filed: November 12, 2015
    Publication date: April 28, 2016
    Inventor: Ashutosh CHILKOTI
  • THERAPEUTIC AGENTS COMPRISING ELASTIN-LIKE PEPTIDES
    Publication number: 20160030521
    Abstract: The present invention provides therapeutic agents and compositions comprising elastin-like peptides (ELPs) and therapeutic proteins. In some embodiments, the therapeutic protein is a GLP-1 receptor agonist, insulin, or Factor VII/VIIa, including functional analogs. The present invention further provides encoding polynucleotides, as well as methods of making and using the therapeutic agents. The therapeutic agents have improvements in relation to their use as therapeutics, including, inter alia, one or more of half-life, clearance and/or persistance in the body, solubility, and bioavailability.
    Type: Application
    Filed: August 10, 2015
    Publication date: February 4, 2016
    Inventor: Ashutosh CHILKOTI
  • Methods of treating diabetes using therapeutic agents comprising a GLP-1 receptor agonist and elastin-like peptides
    Patent number: 9200083
    Abstract: The present invention provides therapeutic agents and compositions comprising elastin-like peptides (ELPs) and therapeutic proteins. In some embodiments, the therapeutic protein is a GLP-1 receptor agonist, insulin, or Factor VII/VIIa, including functional analogs. The present invention further provides encoding polynucleotides, as well as methods of making and using the therapeutic agents. The therapeutic agents have improvements in relation to their use as therapeutics, including, inter alia, one or more of half-life, clearance and/or persistance in the body, solubility, and bioavailability.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: December 1, 2015
    Assignee: DUKE UNIVERSITY
    Inventor: Ashutosh Chilkoti
  • Therapeutic agents comprising insulin and elastin-like peptides
    Patent number: 9127047
    Abstract: The present invention provides therapeutic agents and compositions comprising elastin-like peptides (ELPs) and therapeutic proteins. In some embodiments, the therapeutic protein is a GLP-1 receptor agonist, insulin, or Factor VII/VIIa, including functional analogs. The present invention further provides encoding polynucleotides, as well as methods of making and using the therapeutic agents. The therapeutic agents have improvements in relation to their use as therapeutics, including, inter alia, one or more of half-life, clearance and/or persistence in the body, solubility, and bioavailability.
    Type: Grant
    Filed: June 15, 2012
    Date of Patent: September 8, 2015
    Assignee: DUKE UNIVERSITY
    Inventor: Ashutosh Chilkoti