Abstract: The claimed invention is directed towards a novel combination cell electroporation/cell culturing apparatus which can be termed a cell culture dish suitable for in vitro electroporation, and towards a device suitable for in vivo electroporation—both useful in methods suitable for the generation of developmentally-activated, pluripotent, pluripotent-like, multipotent, and/or self-renewing cells which are capable of beginning to differentiate in culture into a variety of cell types and capable of further differentiation in vivo. The claimed invention is also directed towards the generation of desirable, differentiating somatic cell populations transplantable to animals or patients, genetic modification of endogenous and exogenous cells, and the treatment of patients suffering from diseases that may be ameliorated by these methods. This invention also provides methods for preventing, treating, or retarding disease, for example, immunodeficiency virus (e.g. HIV-1, HIV-2, SIV, FIV, etc.) infection.

Abstract: Methods and apparatus for determining a unique audience exposed to media while reducing memory resources of a computing device are disclosed herein. Example instructions cause a machine to at least, based on impression requests from a plurality of client devices via a network, log a plurality of impressions corresponding to media accessed at the client devices; obtain a count of demographic impressions logged by a database proprietor; obtain a count of registered users of the database proprietor exposed to the media; and execute a process to determine a unique audience size by multiplying a count of the plurality of impressions by a square of the count of the registered users to generate a product; dividing the product by the count of the demographic impressions to generate a quotient; and determining the unique audience size based on a square root of the quotient.

Abstract: Methods, apparatus, systems and articles of manufacture are disclosed to estimate demographics of users employing social media. An example method disclosed herein includes (1) identifying a social media message regarding an asset, the social media message associated with a user identifier associated with the user, (2) determining demographics associated with a group of people exposed to the asset, (3) associating the user identifier with the asset, and (4) repeating (1) to (3). The example method also includes (5) combining demographics associated with two or more different groups of people with which the user identifier is associated to estimate a demographic profile for the user.

Abstract: A cell culture dish made of non media-permeable material and having a base and a plurality of separate juxtaposed side-by-side wells having common interior well walls preventing physical contact or movement of chemical factors between the separated cell or tissue cultures, the walls having different heights and defining an outer “surround” cell or tissue culture and one or more inner “center” cell or tissue cultures to enable contained cell or tissue communication between the well spaces, the wells configured to comprise two or more cell or tissue cultures, the wells permitting signal communication between the cells or tissues situated within said wells, and further comprising wall material, electrode contacts electrodes and/or electrode contacts, and well dimensions suitable for facilitating electroporation. The inventor observed 100% efficiency in cell reprogramming with protein electroporation-orders of magnitude above other reported methods.

Abstract: Methods and apparatus for determining a unique audience exposed to media while reducing memory resources of a computing device are disclosed herein. Example instructions cause a machine to at least, based on impression requests from a plurality of client devices via a network, log a plurality of impressions corresponding to media accessed at the client devices; obtain a count of demographic impressions logged by a database proprietor; obtain a count of registered users of the database proprietor exposed to the media; and execute a process to determine a unique audience size by multiplying a count of the plurality of impressions by a square of the count of the registered users to generate a product; dividing the product by the count of the demographic impressions to generate a quotient; and determining the unique audience size based on a square root of the quotient.

Abstract: Methods, apparatus, systems and articles of manufacture are disclosed to estimate demographics of users employing social media. An example method disclosed herein includes (1) identifying a social media message regarding an asset, the social media message associated with a user identifier associated with the user, (2) determining demographics associated with a group of people exposed to the asset, (3) associating the user identifier with the asset, and (4) repeating (1) to (3). The example method also includes (5) combining demographics associated with two or more different groups of people with which the user identifier is associated to estimate a demographic profile for the user.

Abstract: Methods, apparatus, systems and articles of manufacture are disclosed to identify co-relationships between media using social media. An example apparatus includes an audience estimator to: estimate a first audience of first media based on a first set of media-exposure social media messages corresponding to client devices referencing the first media, and estimate a second audience of second media based on a second set of media-exposure social media messages corresponding to the client devices referencing the second media.

Abstract: Methods and apparatus for determining a unique audience exposed to media while reducing memory resources of a computing device are disclosed herein. An example method includes receiving impression requests at a server from a plurality of client devices via a network; based on the impression requests, logging, with the server, a plurality of impressions corresponding to the media accessed at the client devices; obtaining a number of demographic impressions logged by a database proprietor; obtaining a number of registered users of the database proprietor exposed to the media; multiplying, by executing an instruction with a processor, a number of the plurality of impressions by a square of the number of the registered users to generate a product; dividing, by executing an instruction with the processor, the product by a number of the demographic impressions to generate a quotient; and determining, by executing an instruction with the processor, the unique audience based on a square root of the quotient.

Abstract: Methods, apparatus, systems and articles of manufacture are disclosed to identify co-relationships between media using social media. An example method includes obtaining media-exposure social media messages based on keywords and posting timestamps, and identifying a media-pairing including first media and second media. The example method also includes estimating a first audience of the first media based on a first set of media-exposure social media messages referencing the first media, and estimating a second audience of the second media based on a second set of media-exposure social media messages referencing the second media.

Abstract: The present invention relates to a novel culturing apparatus (assembly) which can be termed a cell culture dish. More particularly, the present invention is a “combined cell culture dish” or “dish-in-dish” apparatus comprising at least one smaller cell culture dish fixedly positioned within a larger cell culture dish, and the number of such fixated cell culture dishes can include a multiple number of fixated cell culture dishes within one another, either concentric or eccentric, in any number of geometric shapes, and without limitation to the number of petri dishes included. An alternate embodiment of this invention can include a plurality of cell culture dishes juxtaposed side-by-side having common interior well walls, and the well walls may or may not be different in height depending on the application.

Abstract: In some embodiments, a method determines characteristics of a user that is viewing a video and analyzes presentation data describing products within a presentation. A product opportunity is selected based on the characteristics of the user and the presentation data for a product. The method selects first metadata for the presentation data and second metadata for the product associated with the presentation data. Then, the method dynamically inserts the first metadata for the presentation data and second metadata for the product into a portion of the video and sends the portion of the video to the user. The product is displayed in the video using the presentation data at the product opportunity.

Abstract: Viewers of presentations of entertainment, news, education and other the like, often observe unadvertised products of interest therein. However, in order to avoid degrading the artistic or objective integrity, producers and distributors of such presentations, do not provide sufficient product description information to permit the viewer to identify and/or purchase the product. The present invention describes methods and systems for permitting such users to research and/or purchase products observed to be involved in presentations by accessing presentation appearance data, product description data, and issuance data prepared, stored and associated in a non-transitory computer-readable database.

Abstract: The present invention departs from using traditional modes of cell-to-cell communication and concerns exerting influence on a first cell or cell population by bringing a second cell or cell population into proximity with the first cell population without the use of mediating diffusible factors. Cell to cell communications traditionally occur by way of a variety of mechanisms including, for example, by direct coupling through gap junctions using antigen presentation or using ligand receptor interactions.

Abstract: Methods and apparatus for over the top (OTT) media measurement are disclosed herein. Example methods include comparing, with an on-site meter in communication with an OTT service provider server, household data maintained by the OTT service provider server with anonymized panelist data provided by an audience measurement entity to identify a first person predicted to be included in a first household according to the anonymized panelist data but not included in the first household according to the household data, accessing media impressions collected by the OTT service provider server and corresponding to media accessed with a first OTT device associated with the first household, and crediting, with the on-site meter, usage of the first OTT device associated with access of the media to the first person based on the media impressions and demographic data obtained from the anonymized panelist data and attributed to the first person.

Abstract: Methods, apparatus, systems and articles of manufacture are disclosed to identify co-relationships between media using social media. An example method includes obtaining media-exposure social media messages based on keywords and posting timestamps, and identifying a media-pairing including first media and second media. The example method also includes estimating a first audience of the first media based on a first set of media-exposure social media messages referencing the first media, and estimating a second audience of the second media based on a second set of media-exposure social media messages referencing the second media.

Abstract: Methods and apparatus for over the top (OTT) media measurement are disclosed herein. Example methods include comparing, with an on-site meter in communication with an OTT service provider server, household data maintained by the OTT service provider server with anonymized panelist data provided by an audience measurement entity to identify a first person predicted to be included in a first household according to the anonymized panelist data but not included in the first household according to the household data, accessing media impressions collected by the OTT service provider server and corresponding to media accessed with a first OTT device associated with the first household, and crediting, with the on-site meter, usage of the first OTT device associated with access of the media to the first person based on the media impressions and demographic data obtained from the anonymized panelist data and attributed to the first person.

Abstract: Methods, apparatus, systems and articles of manufacture are disclosed to estimate an unknown audience size from recorded impressions for an online media. The estimate of the unknown audience size for the online media is based on a total number of initial impressions and a frequency distribution of recorded demographic impressions across a partial audience size for the online media. The estimate of the unknown audience size is determined by modeling the probability of obtaining the frequency distribution of the recorded demographic impressions across the partial audience for different possible unknown audience sizes in a range of unknown audience sizes and determining an estimate for the unknown audience size by evaluating the models for the different possible unknown audience sizes.

Abstract: Methods and apparatus to correct for deterioration of a demographic model to associate demographic information with media impression information are disclosed. An example method includes estimating first and second ages of audience members based on demographic information; estimating a third age of an audience member who is not included in the audience members; applying a window function to the second ages to determine a distribution of ages based on the third age; multiplying window values by the first ages to determine corrected first age components; dividing a total of the corrected first age components by a sum of the window values to determine an estimated age of the audience member at a first time; and determining the corrected age of the audience member at a second time based on the estimated age of the audience member at the first time and a time difference between the first and second times.

Abstract: The present invention relates to a novel culturing apparatus (assembly) which can be termed a cell culture dish. More particularly, the present invention is a “combined cell culture dish” or “dish-in-dish” apparatus comprising at least one smaller cell culture dish fixedly positioned within a larger cell culture dish, and the number of such fixated cell culture dishes can include a multiple number of fixated cell culture dishes within one another, either concentric or eccentric, in any number of geometric shapes, and without limitation to the number of petri dishes included. An alternate embodiment of this invention can include a plurality of cell culture dishes juxtaposed side-by-side having common interior well walls, and the well walls may or may not be different in height depending on the application.

Abstract: The present invention departs from using traditional modes of cell-to-cell communication and concerns exerting influence on a first cell or cell population by bringing a second cell or cell population into proximity with the first cell population without the use of mediating diffusible factors. Cell to cell communications traditionally occur by way of a variety of mechanisms including, for example, by direct coupling through gap junctions using antigen presentation or using ligand receptor interactions.