Patents by Inventor Bahram Jalali
Bahram Jalali has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20210255088Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.Type: ApplicationFiled: October 12, 2020Publication date: August 19, 2021Inventors: Bahram Jalali, Eric D. Diebold, Brandon Buckley
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Patent number: 11009459Abstract: An apparatus and methods for high-speed non-linear spectrally encoded multi-photon imaging that are particularly suited for use in two photon fluorescence and fluorescence lifetime imaging. The system is capable of optical image compression and scale invariant digital zoom. A wavelength agile laser with digitally synthesized electro-optic modulation in a master oscillator-power amplifier configuration is combined with spectral encoding to eliminate the speed limitations of inertial scanning. The technique for fast two photon fluorescent imaging with simultaneous lifetime imaging independently detects the location, amplitude and lifetime of fluorescent emission by synthesizing a sequential excitation beam via digital electro-optic modulation of a quasi-CW swept source followed by time encoded detection. For fluorescent imaging, spectral and temporal mappings are employed separately, with quasi-CW spectral encoding used for pumping and time encoding for constructing the image at fluorescence wavelength.Type: GrantFiled: December 27, 2018Date of Patent: May 18, 2021Assignees: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, UNIVERSITAET ZU LUEBECKInventors: Sebastian Karpf, Bahram Jalali, Robert Huber
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Patent number: 10845295Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.Type: GrantFiled: July 31, 2019Date of Patent: November 24, 2020Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Bahram Jalali, Eric D. Diebold, Brandon Buckley
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Publication number: 20200311532Abstract: A Lambda reservoir computing system that can readily handle shifts in the distribution of input and output data. Data is modulated onto the spectrum of a broadband optical pulse which is subjected to nonlinear optical effects transforming the data to a higher optical dimensional space. The optical information is converted to electronic signals for processing by an electronic machine learning stage which then generates an output based on the data processed by the learning stage.Type: ApplicationFiled: March 31, 2020Publication date: October 1, 2020Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Bahram Jalali, Tingyi Zhou, Cejo Konuparamban Lonappan
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Patent number: 10746630Abstract: Apparatus and/or method for performing single-shot network analysis of electrical, electronic and electro-optical elements (e.g., components, circuits, modules, sub-systems and/or systems) on a device, or devices, under test (DUT). A pulsed optical source is directed through a first dispersion element to an modulator, while a delayed version of the pulsed optical source is directed to the DUT (pulsed optical source converted to electrical signal if DUT has electrical input), whose electrical output is fed to the modulator whose modulated optical pulse output is stretched through a second optical dispersion element, then converted to an electrical signal and processed to provide analysis and/or display of DUT response.Type: GrantFiled: December 19, 2018Date of Patent: August 18, 2020Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Bahram Jalali, Cejo Lonappan, Asad Madni
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Patent number: 10593039Abstract: A method and apparatus for using deep learning in label-free cell classification and machine vision extraction of particles. A time stretch quantitative phase imaging (TS-QPI) system is described which provides high-throughput quantitative imaging, and utilizing photonic time stretching. In at least one embodiment, TS-QPI is integrated with deep learning to achieve record high accuracies in label-free cell classification. The system captures quantitative optical phase and intensity images and extracts multiple biophysical features of individual cells. These biophysical measurements form a hyperdimensional feature space in which supervised learning is performed for cell classification. The system is particularly well suited for data-driven phenotypic diagnosis and improved understanding of heterogeneous gene expression in cells.Type: GrantFiled: March 22, 2018Date of Patent: March 17, 2020Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Bahram Jalali, Ata Mahjoubfar, Lifan Chen
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Publication number: 20190376887Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.Type: ApplicationFiled: July 31, 2019Publication date: December 12, 2019Inventors: Bahram Jalali, Eric D. Diebold, Brandon Buckley
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Publication number: 20190376894Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.Type: ApplicationFiled: July 31, 2019Publication date: December 12, 2019Inventors: Eric D. Diebold, Bahram Jalali, Brandon Buckley
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Publication number: 20190376895Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.Type: ApplicationFiled: July 31, 2019Publication date: December 12, 2019Inventors: Eric D. Diebold, Bahram Jalali, Brandon Buckley
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Patent number: 10451538Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.Type: GrantFiled: January 14, 2019Date of Patent: October 22, 2019Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Bahram Jalali, Eric Diebold, Brandon Buckley
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Publication number: 20190279369Abstract: Object and shape detection in digital images utilizing edge detection is described. In a first edge detection approach, phase transformation is utilized in the frequency domain, such as in response to Fourier transform, followed by use of a frequency-domain phase kernel and inverse-Fourier transform. Edge detection is also provided using a phase transform in the spatial domain utilizing a convolution approach. In a second edge detection approach, phase stretching is utilized, such as in combination with phase histogramming along with thresholding and morphological operations. Numerous example images are provided illustrating benefits of the disclosed technology with different applications and under different conditions.Type: ApplicationFiled: March 19, 2019Publication date: September 12, 2019Applicant: The Regents of the University of CaliforniaInventors: Bahram Jalali, Mohammadhossein Asghari
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Patent number: 10408758Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.Type: GrantFiled: April 24, 2018Date of Patent: September 10, 2019Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Eric D. Diebold, Bahram Jalali, Brandon Buckley
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Publication number: 20190226989Abstract: An apparatus and methods for high-speed non-linear spectrally encoded multi-photon imaging that are particularly suited for use in two photon fluorescence and fluorescence lifetime imaging. The system is capable of optical image compression and scale invariant digital zoom. A wavelength agile laser with digitally synthesized electro-optic modulation in a master oscillator-power amplifier configuration is combined with spectral encoding to eliminate the speed limitations of inertial scanning. The technique for fast two photon fluorescent imaging with simultaneous lifetime imaging independently detects the location, amplitude and lifetime of fluorescent emission by synthesizing a sequential excitation beam via digital electro-optic modulation of a quasi-CW swept source followed by time encoded detection. For fluorescent imaging, spectral and temporal mappings are employed separately, with quasi-CW spectral encoding used for pumping and time encoding for constructing the image at fluorescence wavelength.Type: ApplicationFiled: December 27, 2018Publication date: July 25, 2019Applicants: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, UNIVERSITAET ZU LUEBECKInventors: Sebastian Karpf, Bahram Jalali, Robert Huber
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Publication number: 20190219480Abstract: Apparatus and/or method for performing single-shot network analysis of electrical, electronic and electro-optical elements (e.g., components, circuits, modules, sub-systems and/or systems) on a device, or devices, under test (DUT). A pulsed optical source is directed through a first dispersion element to an modulator, while a delayed version of the pulsed optical source is directed to the DUT (pulsed optical source converted to electrical signal if DUT has electrical input), whose electrical output is fed to the modulator whose modulated optical pulse output is stretched through a second optical dispersion element, then converted to an electrical signal and processed to provide analysis and/or display of DUT response.Type: ApplicationFiled: December 19, 2018Publication date: July 18, 2019Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Bahram Jalali, Cejo Lonappan, Asad Madni
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Publication number: 20190145882Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.Type: ApplicationFiled: January 14, 2019Publication date: May 16, 2019Inventors: Bahram Jalali, Eric Diebold, Brandon Buckley
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Patent number: 10275891Abstract: Object and shape detection in digital images utilizing edge detection is described. In a first edge detection approach, phase transformation is utilized in the frequency domain, such as in response to Fourier transform, followed by use of a frequency-domain phase kernel and inverse-Fourier transform. Edge detection is also provided using a phase transform in the spatial domain utilizing a convolution approach. In a second edge detection approach, phase stretching is utilized, such as in combination with phase histogramming along with thresholding and morphological operations. Numerous example images are provided illustrating benefits of the disclosed technology with different applications and under different conditions.Type: GrantFiled: November 2, 2016Date of Patent: April 30, 2019Assignee: The Regents of the University of CaliforniaInventors: Bahram Jalali, Mohammadhossein Asghari
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Patent number: 10222316Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.Type: GrantFiled: June 26, 2018Date of Patent: March 5, 2019Assignee: The Regents of the University of CaliforniaInventors: Bahram Jalali, Eric Diebold, Brandon Buckley
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Publication number: 20180364146Abstract: An imaging flow cytometry apparatus and method which allows registering multiple locations across a cell, and/or across multiple flow channels, in parallel using radio-frequency-tagged emission (FIRE) coupled with a parallel optical detection scheme toward increasing analysis throughput. An optical source is modulated by multiple RF frequencies to produce an optical interrogation beam having a spatially distributed beat frequency. This beam is directed to one or more focused streams of cells whose responsive fluorescence, in different frequencies, is registered in parallel by an optical detector.Type: ApplicationFiled: June 26, 2018Publication date: December 20, 2018Inventors: Bahram Jalali, Eric Diebold, Brandon Buckley
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Publication number: 20180286038Abstract: A method and apparatus for using deep learning in label-free cell classification and machine vision extraction of particles. A time stretch quantitative phase imaging (TS-QPI) system is described which provides high-throughput quantitative imaging, and utilizing photonic time stretching. In at least one embodiment, TS-QPI is integrated with deep learning to achieve record high accuracies in label-free cell classification. The system captures quantitative optical phase and intensity images and extracts multiple biophysical features of individual cells. These biophysical measurements form a hyperdimensional feature space in which supervised learning is performed for cell classification. The system is particularly well suited for data-driven phenotypic diagnosis and improved understanding of heterogeneous gene expression in cells.Type: ApplicationFiled: March 22, 2018Publication date: October 4, 2018Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Bahram Jalali, Ata Mahjoubfar, Lifan Chen
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Publication number: 20180246037Abstract: Apparatus and methods for fluorescence imaging using radiofrequency multiplexed excitation. One apparatus splits an excitation laser beam into two arms of a Mach-Zehnder interferometer. The light in the first beam is frequency shifted by an acousto-optic deflector, which is driven by a phase-engineered radiofrequency comb designed to minimize peak-to-average power ratio. This RF comb generates multiple deflected optical beams possessing a range of output angles and frequency shifts. The second beam is shifted in frequency using an acousto-optic frequency shifter. After combining at a second beam splitter, the two beams are focused to a line on the sample using a conventional laser scanning microscope lens system. The acousto-optic deflectors frequency-encode the simultaneous excitation of an entire row of pixels, which enables detection and de-multiplexing of fluorescence images using a single photomultiplier tube and digital phase-coherent signal recovery techniques.Type: ApplicationFiled: April 24, 2018Publication date: August 30, 2018Inventors: Eric D. Diebold, Bahram Jalali, Brandon Buckley