Abstract: A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

Abstract: Presented are methods and compositions for obtaining sequence information from one or more individual cells. The methods are useful for obtaining sequence information for a single nucleotide sequence, and for multiplex generation of sequence information from one or more individual cells.

Abstract: The present invention relates to the field of material determination or analysis, and provides a method of assessing drying depth of cementitious materials including the following steps: preparing a plurality of cementitious material specimens; drying the cementitious material specimens; conducting electrochemical impedance spectrum measurement on each cementitious material specimen; accordingly determining a model for analyzing the drying depth of the cementitious material specimen. The method of assessing drying depth of the cementitious material provided by the present invention takes the influence of the resistivity changes on the model into consideration, and the drying depth of the cementitious material is reflected and reckoned with regularly changes of the electrochemical parameters, not only increasing the accuracy but also saving much testing labor, time and cost.

Abstract: Disclosed is a gene expression panel that can be used to predict prostate cancer (PCa) progression. Some embodiments provide methods for predicting clinical recurrence of PCa. Some embodiments provide a method for predicting progression of prostate cancer in an individual, the method comprising: (a) receiving expression levels of a collection of signature genes from a biological sample taken from said individual, wherein said collection of signature genes comprises at least two genes selected from the group consisting of: NKX2-1, UPK1A, ADRA2C, ABCC11, MMP11, CPVL, ZYG11A, CLEC4F, OAS2, PGC, UPK3B, PCBP3, ABLIM1, EDARADD, GPR81, MYBPC1, F10, KCNA3, GLDC, KCNQ2, RAPGEF1, TUBB2B, MB, DUOXA1, C2orf43, DUOX1, PCA3 and NPR3; (b) applying the expression levels to a predictive model relating expression levels of said collection of signature genes with prostate cancer progression; and (c) evaluating an output of said predictive model to predict progression of prostate cancer in said individual.

Abstract: A computing system includes an array of storage devices configured to store storage data and check data; and a storage engine, coupled to the array of storage devices, configured to: access the storage data, detect an erasure storage data, an erasure check data, or a combination thereof when the storage data is not accessible from the array of storage devices, and recover the storage data from the erasure storage data, the erasure check data, or the combination thereof by applying at least one of a block of check data including a slope from the check data.

Abstract: A computing system includes an array of storage devices configured to provide access to storage data and check data corresponding to a data block length; and a storage engine, coupled to the array of storage devices, configured to: detect one more erroneous data, one more erroneous check data, or a combination thereof for representing the storage data, the check data, or a combination thereof inaccessible or failing a status check process after initial storage thereof, determine a prime shift factor for representing a smallest prime number not less than a block unit-quantity, wherein the block unit-quantity is for representing a quantity of shift units within the data block length, and iteratively generate a target recovery set based on a circular-shift mechanism utilizing the prime shift factor for recovering the one more erroneous data, the one more erroneous check data, or a combination thereof.

Abstract: Disclosed is a gene expression panel that can be used to predict prostate cancer (PCa) progression. Some embodiments provide methods for predicting clinical recurrence of PCa. Some embodiments provide a method for predicting progression of prostate cancer in an individual, the method comprising: (a) receiving expression levels of a collection of signature genes from a biological sample taken from said individual, wherein said collection of signature genes comprises at least two genes selected from the group consisting of: NKX2-1, UPK1A, ADRA2C, ABCC11, MMP11, CPVL, ZYG11A, CLEC4F, OAS2, PGC, UPK3B, PCBP3, ABLIM1, EDARADD, GPR81, MYBPC1, F10, KCNA3, GLDC, KCNQ2, RAPGEF1, TUBB2B, MB, DUOXA1, C2orf43, DUOX1, PCA3 and NPR3; (b) applying the expression levels to a predictive model relating expression levels of said collection of signature genes with prostate cancer progression; and (c) evaluating an output of said predictive model to predict progression of prostate cancer in said individual.

Abstract: Presented herein are methods and compositions for determining haplotypes in a sample. The methods are useful for obtaining sequence information regarding, for example, HLA type and haplotype. Also presented herein are methods of determining haplotypes in a sample based on a plurality sequence reads.

Abstract: Disclosed is a zoom objective lens (16) of an operating microscope (10), wherein the zoom objective lens (16) and a lens cone of the operating microscope (10) are provided along a same axis (24). The zoom objective lens (16) comprises a positive lens group (11) and a negative lens group (12) provided along a same axis (24). The positive lens group (11) is provided on an objective lens holder (14), and the negative lens group (12) is provided on a negative lens group holder (15) and directly faces the object under observation (G). The zoom objective lens (16) is changeably connected to a body (20) of the operating microscope through the objective lens holder (14) or a coupling holder, the negative lens group holder (15) is capable of moving in the optical axis (24) direction relative to the objective lens holder (14), and the position of the objective lens holder (14) relative to an illumination system (21) and an observation system (22) remains unchanged.

Abstract: The present invention relates to the field of material determination or analysis, and provides a method of assessing drying depth of cementitious materials including the following steps: preparing a plurality of cementitious material specimens; drying the cementitious material specimens; conducting electrochemical impedance spectrum measurement on each cementitious material specimen; accordingly determining a model for analyzing the drying depth of the cementitious material specimen. The method of assessing drying depth of the cementitious material provided by the present invention takes the influence of the resistivity changes on the model into consideration, and the drying depth of the cementitious material is reflected and reckoned with regularly changes of the electrochemical parameters, not only increasing the accuracy but also saving much testing labor, time and cost.

Abstract: A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

Abstract: The disclosure provides methods of DNA amplification mediated by DNA ligase. Specifically disclosed is a method of amplifying a target region at DNA level, which comprises repeating cycles of amplification comprising the following steps: denaturing DNA template to obtain single target DNA strands; hybridizing a primer pair comprising an upstream primer and a downstream primer to the target DNA strand, wherein the upstream primer is hybridized to a first nucleic acid sequence of the target region, and the downstream primer is hybridized to a second nucleic acid sequence of the target region; the first nucleic acid sequence is downstream to the second nucleic acid sequence on the target DNA strand, with the downstream primer containing a phosphorylated 5? end; ligating the upstream primer or extension product thereof to the downstream primer or extension product thereof, to obtain a semi-amplification product. This disclosure also discloses a kit used to amplify a target region at DNA level.

Abstract: A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

Abstract: A card connector includes an insulative housing, a plurality of contacts received in the insulative housing and a metallic cover covering the insulative housing. The contact includes a retaining portion, an extending portion horizontally and forwardly extending from the retaining portion, a connecting portion downwardly and forwardly aslant extending from the extending portion, a floating portion downwardly from the connecting portion and a contacting portion further downwardly extending from the floating portion. There are two bending points on linking portions of the connecting portion connecting with the floating portion and the extending portion, respectively. By such arrangement, when the electrical card is inserted, the contact can rotate about the two bending points in turn, that can improve an elasticity of the contact.

Abstract: The present invention relates to a method for preparing basic zinc chloride, comprising the following steps: A: preparing raw materials: preparing zinc chloride solution, ammonia water and an induction system; B: performing synthesis: adding the zinc chloride solution and the ammonia water into the induction system in a parallel flow manner, and controlling the temperature to be 60.0-90.0° C.; after the feeding is finished, continuing to react for 20.0-40.0 minutes; and C: performing filtration, washing and drying: after filtering and washing the synthesized basic zinc chloride, drying the basic zinc chloride for 4.0-8.0 hours at 80-105° C. to obtain the basic zinc chloride product. Compared with the prior art, the method for preparing basic zinc chloride has such advantages as simple process, low impurity content, easy-to-control product quality, and suitability for industrialization.

Abstract: The present application discloses a method of detecting the methylation of specific genome regions in a DNA fragment from a sample containing DNA. The method includes treating the DNA fragment containing specific genome regions with sodium bisulfite and obtaining single-strand DNA fragment; attaching an adapter to one or both ends of the single-strand DNA fragment; optionally cyclizing the adapter-attached single-strand DNA fragment; preparing the single-strand DNA fragment with attached adapter into a DNA sequencing library containing the specific genome regions; sequencing the DNA sequencing library to identify the sequence of the single-strand DNA fragment. The present application also discloses a kit for detecting the methylation of specific genome regions in a DNA fragment from a sample containing DNA, and the use of single strand ligating agent in preparing a kit for detecting the methylation of specific genome regions in a DNA fragment.

Abstract: A computing system includes an array of storage devices configured to store storage data and check data; and a storage engine, coupled to the array of storage devices, configured to: access the storage data, detect an erasure storage data, an erasure check data, or a combination thereof when the storage data is not accessible from the array of storage devices, and recover the storage data from the erasure storage data, the erasure check data, or the combination thereof by applying at least one of a block of check data including a slope from the check data.

Abstract: A computing system includes an array of storage devices configured to provide access to storage data and check data corresponding to a data block length; and a storage engine, coupled to the array of storage devices, configured to: detect one more erroneous data, one more erroneous check data, or a combination thereof for representing the storage data, the check data, or a combination thereof inaccessible or failing a status check process after initial storage thereof, determine a prime shift factor for representing a smallest prime number not less than a block unit-quantity, wherein the block unit-quantity is for representing a quantity of shift units within the data block length, and iteratively generate a target recovery set based on a circular-shift mechanism utilizing the prime shift factor for recovering the one more erroneous data, the one more erroneous check data, or a combination thereof.

Abstract: The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.

Abstract: Disclosed is a zoom objective lens (16) of an operating microscope (10), wherein the zoom objective lens (16) and a lens cone of the operating microscope (10) are provided along a same axis (24). The zoom objective lens (16) comprises a positive lens group (11) and a negative lens group (12) provided along a same axis (24). The positive lens group (11) is provided on an objective lens holder (14), and the negative lens group (12) is provided on a negative lens group holder (15) and directly faces the object under observation (G). The zoom objective lens (16) is changeably connected to a body (20) of the operating microscope through the objective lens holder (14) or a coupling holder, the negative lens group holder (15) is capable of moving in the optical axis (24) direction relative to the objective lens holder (14), and the position of the objective lens holder (14) relative to an illumination system (21) and an observation system (22) remains unchanged.