Abstract: Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

Abstract: Disclosed is a gene expression panel that can be used to predict prostate cancer (PCa) progression. Some embodiments provide methods for predicting clinical recurrence of PCa. Some embodiments provide a method for predicting progression of prostate cancer in an individual, the method comprising: (a) receiving expression levels of a collection of signature genes from a biological sample taken from said individual, wherein said collection of signature genes comprises at least two genes selected from the group consisting of: NKX2-1, UPK1A, ADRA2C, ABCC11, MMP11, CPVL, ZYG11A, CLEC4F, OAS2, PGC, UPK3B, PCBP3, ABLIM1, EDARADD, GPR81, MYBPC1, F10, KCNA3, GLDC, KCNQ2, RAPGEF1, TUBB2B, MB, DUOXA1, C2orf43, DUOX1, PCA3 and NPR3; (b) applying the expression levels to a predictive model relating expression levels of said collection of signature genes with prostate cancer progression; and (c) evaluating an output of said predictive model to predict progression of prostate cancer in said individual.

Abstract: The present invention relates to a method for preparing basic zinc chloride, comprising the following steps: A: preparing raw materials: preparing zinc chloride solution, ammonia water and an induction system; B: performing synthesis: adding the zinc chloride solution and the ammonia water into the induction system in a parallel flow manner, and controlling the temperature to be 60.0-90.0° C.; after the feeding is finished, continuing to react for 20.0-40.0 minutes; and C: performing filtration, washing and drying: after filtering and washing the synthesized basic zinc chloride, drying the basic zinc chloride for 4.0-8.0 hours at 80-105° C. to obtain the basic zinc chloride product. Compared with the prior art, the method for preparing basic zinc chloride has such advantages as simple process, low impurity content, easy-to-control product quality, and suitability for industrialization.

Abstract: The present invention is directed to methods and compositions for the use of micro sphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.

Abstract: An electronic device includes a housing and a display module received in the housing. The display module includes a display panel, a touch panel positioned on the display panel, a light guide plate positioned on the touch panel, a light source positioned at a side of the light guide plate, and a hardened layer positioned on the light guide plate. The light guide plate and the display panel are respectively positioned at opposite sides of the touch panel. The hardened layer covers a surface of the light guide plate away from the touch panel.

Abstract: An electrical connector, for receiving an electrical card, includes an insulative housing, a plurality of conductive contacts retained in the insulative housing and a metallic shell covering the insulative housing. The metallic cover and the insulative housing define a receiving cavity. The shell has a top plate, the top plate is formed with a plurality of openings, a plurality of resisting pieces bent downwardly from front edges of the openings, and a plurality of gaps defined between each two adjacent resisting pieces. Each contact has a conductive portion extending into the receiving cavity, and the conductive portions and the gaps of the top plate are alternatively disposed.

Abstract: Presented herein are methods and compositions for determining haplotypes in a sample. The methods are useful for obtaining sequence information regarding, for example, HLA type and haplotype. Also presented herein are methods of determining haplotypes in a sample based on a plurality sequence reads.

Abstract: An electrical connector comprises an insulative block, a plurality of contacts received in the insulative block. The contacts each include a retaining portion retaining in the insulative block, a contacting portion extending forwardly from the retaining portion and a soldering portion. The contacts include a plurality of differential signal contacts and a plurality of grounding contacts. The soldering portion of differential signal contacts arranged to a plurality of rows and the soldering portion of grounding contacts arranged to a plurality of another rows which parallel to the row of the differential contacts. The row of the differential contacts and the row of the grounding contacts are spaced from each other.

Abstract: The present invention is directed to methods and compositions for the use of micro sphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.

Abstract: Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

Abstract: Presented are methods and compositions for obtaining sequence information from one or more individual cells. The methods are useful for obtaining sequence information for a single nucleotide sequence, and for multiplex generation of sequence information from one or more individual cells.

Abstract: The present disclosure relates to systems and methods for the amplification of nucleic acids, including, but not limited to, the amplification of nucleic acid libraries and whole genome amplification.

Abstract: Presented herein are methods and compositions for determining haplotypes in a sample. The methods are useful for obtaining sequence information regarding, for example, HLA type and haplotype. Also presented herein are methods of determining haplotypes in a sample based on a plurality sequence reads.

Abstract: An electrical card connector adapted for receiving an electrical card, comprises an insulative housing, a plurality of contacts received in the insulative housing, and a shell covering the insulative housing. The shell includes an upper shell with a top wall and a lower shell, the upper shell and the insulative housing define a space for the electrical card. The electrical card connector further has an eject mechanism mounted on the top wall. The eject mechanism has an eject member and a push member, the eject member has a fixing arm, the push member has a mounting wall fixed with the fixing arm to fix the push member to the eject member. Such arrangement can reduce the difficulty of molding process and the manufacturing costs of the electrical card connector.

Abstract: A card connector assembly includes a card connector and a pick-up cap attached to the card connector. The card connector includes an insulating housing and a plurality of conductive terminals The insulating housing has a base portion, a mating frame and a mating cavity formed in the mating frame. The conductive terminals have contacting sections and soldering sections. The pick-up cap has an adsorption portion and a retaining plate extending downwardly from a middle area of the adsorption portion and inserted into the mating cavity. The retaining plate has an interference portion protruding out of the retaining portion to abut against an inner surface of the mating frame.

Abstract: A card connector includes an insulating housing, a plurality of conductive terminals and a retaining plate. The insulating housing has a base portion, an upper face, a lower face, a mating frame with a mating cavity which has a mating face parallel to the lower face. The conductive terminals have contacting portions extending into the mating cavity. The base portion has a retaining space recessed upwardly from the lower face and a platform located at the upper face of the base portion, and the retaining space upwardly runs through the platform. A stopping portion is formed between the retaining space and the mating frame. The retaining plate is retained in the retaining space and has a plurality of soldering legs extending downwardly out of the lower face and a latch portion extending upwardly and latching with the stopping portion.

Abstract: A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

Abstract: A card connector comprises an insulative housing, a plurality of contacts received in the insulative housing and a metallic cover covering the insulative housing. The contact includes a retaining portion, an extending portion horizontally and forwardly extending from the retaining portion, a connecting portion downwardly and forwardly aslant extending from the extending portion, a floating portion downwardly from the connecting portion and a contacting portion further downwardly extending from the floating portion. There are two bending points on linking portions of the connecting portion connecting with the floating portion and the extending portion, respectively. By such arrangement, when the electrical card is inserted, the contact can rotate about the two bending points in turn, that can improve an elasticity of the contact.

Abstract: A card connector comprises an insulative housing, a plurality of contacts received in the insulative housing and a metallic cover covering the insulative housing. The contact includes a retaining portion, an extending portion horizontally and forwardly extending from the retaining portion, a connecting portion downwardly and forwardly aslant extending from the extending portion, a floating portion downwardly from the connecting portion and a contacting portion further downwardly extending from the floating portion. There are two bending points on linking portions of the connecting portion connecting with the floating portion and the extending portion, respectively. By such arrangement, when the electrical card is inserted, the contact can rotate about the two bending points in turn, that can improve an elasticity of the contact.

Abstract: An electrical connector, for receiving an electrical card, includes an insulative housing, a plurality of conductive contacts retained in the insulative housing and a metallic shell covering the insulative housing. The metallic cover and the insulative housing define a receiving cavity. The shell has a top plate, the top plate is formed with a plurality of openings, a plurality of resisting pieces bent downwardly from front edges of the openings, and a plurality of gaps defined between each two adjacent resisting pieces. Each contact has a conductive portion extending into the receiving cavity, and the conductive portions and the gaps of the top plate are alternatively disposed.