Abstract: A product development technique is provided that enables a user to define a product family design structure. The product family design structure includes a number of customer concerns, a number of physical properties associated with components of the product, and a number of relation models. Each customer concern is associated with at least one physical property via at least one mathematical relationship defined in at least one of the relation models. The technique also enables the user to define one or more product development projects based on the product family design structure, where the one or more product development projects vary from the product family design structure according to one or more overrides specified by the user for the values associated with one or more of the customer concerns, relation models, and/or physical properties defined in the product family design structure.

Abstract: A product design method includes accessing a defined product design structure. The product design structure includes a number of customer concerns, a number of physical properties associated with components of the product, and a number of relation models. Each customer concern is associated with at least one physical property via at least one mathematical relationship defined in at least one of the relation models. The method also includes receiving a value associated with one or more of the physical properties and calculating (using one or more of the relation models) the effect of the received value associated with the one or more physical properties on one or more of the customer concerns. Furthermore, the method includes displaying the calculated effect on the one or more customer concerns and receiving one or more adjustments of the value associated with one or more of the physical properties to create a desired effect on one or more of the customer concerns.

Abstract: High throughput methods for screening genetic variants that are phenotypically distinguishable are provided. Methods for identifying “long-lived” genetic variants among a set of variants are also provided. Methods for identifying pharmaceutical compounds that can promote longevity in various subjects, including mammals, and that can delay the onset of various diseases associated with aging are also provided. Various vectors and host cells containing identified genes/gene products are useful for screening longevity-promoting compounds that can interact with life-span-regulating genes/gene products. Pharmaceutical compositions that can promote longevity are also provided.

Abstract: A dental lamp boom hinge includes a pivot supporting a dental lamp head and a base supporting the pivot where the pivot may be rotated about a substantially vertical axis with respect to the base. The rotational movement between the pivot and the base is damped with a damping device. The damping device in one embodiment is adjustable to provide a variable level of damping.

Abstract: A cabinet and associated method are provided for storage of electrical components. In one embodiment, the cabinet includes a chamber including a number of side walls and a top cap and a canted ceiling formed by the top cap. A first overhang portion is defined by a wide end of the top cap and a second overhang portion is defined by a narrow end of the top cap. A number of first air flow openings are located in the first overhang portion and a number of second air flow openings are located in the second overhang portion. The elevation of at least one of the first air flow openings is at least as great as an elevation of the second air flow openings.

Abstract: Methods of isolating mutant yeast cells with increased life span, as well as mutant yeast cells isolated by the methods, are disclosed. Also described are methods of identifying agents which increase life span of yeast cells, and methods of isolating genes which affect senescence in organisms.

Abstract: The present invention includes an appliance that has a cook vessel and a Peltier device. The Peltier device may have a motive heat distribution system on its waste side. The active side of the Peltier cools and heats the cook vessel in the absence of a motive heat distribution system. The present invention may also operate in the absence of an extraneous temperature regulation system. Methods of cooling and heating food are also part of the invention.

Abstract: Methods of isolating mutant yeast cells with increased life span, as well as mutant yeast cells isolated by the methods, are disclosed. Also described are methods of identifying agents which increase life span of yeast cells, and methods of isolating genes which affect senescence in organisms.

Abstract: The present invention provides mice that have had their PTP-1B genes disrupted by targeted homologous recombination. The mice have no detectable PTP-1B protein, yet appear to be physiologically normal. However, in the fed state on a normal diet, the mice have half the level of circulating insulin as their wild-type littermates. In glucose and insulin tolerance tests, the mice show an increased insulin sensitivity. When fed a high fat, high carbohydrate diet, the mice show a resistance to weight gain as compared to their wild-type littermates. Methods of making the mice and cell lines derived from the mice are also provided. The present invention also provides methods of idendifying inhibitors of the enzymatic activity of PTP-1B as well as inhibitors identified by such methods.

Abstract: The present invention provides mice that have had their PTP-1B genes disrupted by targeted homologous recombination. The mice have no detectable PTP-1B protein, yet appear to be physiologically normal. However, in the fed state on a normal diet, the mice have half the level of circulating insulin as their wild-type littermates. In glucose and insulin tolerance tests, the mice show an increased insulin sensitivity. When fed a high fat, high carbohydrate diet, the mice show a resistance to weight gain as compared to their wild-type littermates. Methods making the mice and cell lines derived from the mice are also provided. The present invention also provides methods of identifying inhibitors of the enzymatic activity of PTP-1B as well as inhibitors identified by such methods.

Abstract: The present invention provides mice that have had their PTP-1B genes disrupted by targeted homologous recombination. The mice have no detectable PTP-1B protein, yet appear to be physiologically normal. However, in the fed state on a normal diet, the mice have half the level of circulating insulin as their wild-type littermates. In glucose and insulin tolerance tests, the mice show an increased insulin sensitivity. When fed a high fat, high carbohydrate diet, the mice show a resistance to weight gain as compared to their wild-type littermates.

Abstract: The invention relates to an intact cell assay for direct quantitation of protein tyrosine phosphatase (PTP) activity using the baculovirus expression system. A PTP expressed in transformed host insect cells is processed and localized in their predicted subcellular compartments. Assays are performed on the PTP expressing host cells challenged with a substrate such as, p-nitrophenyl phosphate. This substrate is hydrolysed to p-nitrophenol by expressed phosphatase activity. Emergence of p-nitrophenol is determined spectrophotometrically and is a measure of PTP activity. Further, the assay and transformed host cells of this invention are particularly useful in a screening method for selecting potential inhibitors to PTPs. Basically, transformed host cells are pre-incubated with or without inhibitors and the assay conducted as described. This invention relates to an intact cell assay with a direct readout of PTP activity that is useful in a cell-based screening method for selecting inhibitors of PTPs.

Abstract: Methods of isolating mutant yeast cells with increased life span, as well as mutant yeast cells isolated by the methods, are disclosed. Also described are methods of identifying agents which increase life span of yeast cells, and methods of isolating genes which affect senescence in organisms.

Abstract: A weld-free heat exchanger assembly core includes a plurality of stacked fin-plate assemblies. A separate enclosure bar is positioned at the end of each fin-plate assembly. A plurality of apertures are pre-drilled in the enclosure bars before assembly of the core. Once the core is assembled, it is brazed to form a unitary structure. Apertures formed in the inlet and outlet manifolds are aligned with apertures formed in the enclosure bars and fasteners are inserted into the aligned apertures. The fasteners serve to draw each manifold into tight engagement with a plurality of separate enclosure bars.

Abstract: Methods of isolating mutant yeast cells with increased life span, as well as mutant yeast cells isolated by the methods, are disclosed. Also described are methods of identifying agents which increase life span of yeast cells, and methods of isolating genes which affect senescence in organisms.

Abstract: Disclosed is a binding assay for proteases and phosphatases, which contain cysteine in their binding sites or as a necessary structural component for enzymatic binding. The sulfhydryl group of cysteine is the nucleophilic group in the enzyme's mechanistic proteolytic and hydrolytic properties. The assay can be used to determine the ability of new, unknown ligands and mixtures of compounds to competitively bind with the enzyme versus a known binding agent for the enzyme, e.g., a known enzyme inhibitor. By the use of a mutant form of the natural or native wild-type enzyme, in which serine, or another amino acid, e.g., alanine, replaces cysteine, the problem of interference from extraneous oxidizing and alkylating agents in the assay procedure is overcome. The interference arises because of oxidation or alkylation of the sulfhydryl, --SH (or --S.sup.-), in the cysteine, which then adversely affects the binding ability of the enzyme.

Abstract: Methods of isolating mutant yeast cells with increased life span, as well as mutant yeast cells isolated by the methods, are disclosed. Also described are methods of identifying agents which increase life span of yeast cells, and methods of isolating genes which affect senescence in organisms.

Abstract: Methods of isolating mutant yeast cells with increased life span, as well as mutant yeast cells isolated by the methods, are disclosed. Also described are methods of identifying agents which increase life span of yeast cells, and methods of isolating genes which affect senescence in organisms.