Patents by Inventor Brian Kobilka
Brian Kobilka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9670266Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR.Type: GrantFiled: April 10, 2015Date of Patent: June 6, 2017Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Brian Kobilka, Daniel Rosenbaum
-
Publication number: 20170153245Abstract: The present invention relates to the field of GPCR structure biology and signaling. In particular, the present invention relates to protein binding domains directed against or capable of specifically binding to a functional conformational state of a G-protein-coupled receptor (GPCR). More specifically, the present invention provides protein binding domains that are capable of increasing the stability of a functional conformational state of a GPCR, in particular, increasing the stability of a GPCR in its active conformational state. The protein binding domains of the present invention can be used as a tool for the structural and functional characterization of G-protein-coupled receptors bound to various natural and synthetic ligands, as well as for screening and drug discovery efforts targeting GPCRs. Moreover, the invention also encompasses the diagnostic, prognostic and therapeutic usefulness of these protein binding domains for GPCR-related diseases.Type: ApplicationFiled: January 18, 2017Publication date: June 1, 2017Applicants: VIB VZW, Vrije Universiteit Brussel, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Jan Steyaert, Els Pardon, Soren G.F. Rasmussen, Juan Jose Fung, Brian Kobilka, Toon Laeremans
-
Patent number: 9556217Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: GrantFiled: November 3, 2015Date of Patent: January 31, 2017Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen
-
Patent number: 9540409Abstract: The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization.Type: GrantFiled: August 7, 2013Date of Patent: January 10, 2017Assignee: WIsconsin Alumni Research FoundationInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasumssen
-
Publication number: 20160347842Abstract: The present invention relates to the field of GPCR structure biology and signaling. In particular, the present invention relates to protein binding domains directed against or capable of specifically binding to a functional conformational state of a G-protein-coupled receptor (GPCR). More specifically, the present invention provides protein binding domains that are capable of increasing the stability of a functional conformational state of a GPCR, in particular, increasing the stability of a GPCR in its active conformational state. The protein binding domains of the present invention can be used as a tool for the structural and functional characterization of G-protein-coupled receptors bound to various natural and synthetic ligands, as well as for screening and drug discovery efforts targeting GPCRs. Moreover, the invention also encompasses the diagnostic, prognostic and therapeutic usefulness of these protein binding domains for GPCR-related diseases.Type: ApplicationFiled: August 13, 2016Publication date: December 1, 2016Applicants: VIB VZW, Vrije Universiteit Brussel, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Jan Steyaert, Juan Jose Fung, Brian Kobilka, Toon Laeremans, Els Pardon, Soren G.F. Rasmussen
-
Patent number: 9453065Abstract: The disclosure relates to the field of GPCR structure biology and signaling. In particular, it relates to protein binding domains directed against or capable of specifically binding to a functional conformational state of a G-protein-coupled receptor (GPCR). More specifically, it provides protein binding domains that are capable of increasing the stability of a functional conformational state of a GPCR, in particular, increasing the stability of a GPCR in its active conformational state. The protein binding domains hereof can be used as a tool for the structural and functional characterization of G-protein-coupled receptors bound to various natural and synthetic ligands, as well as for screening and drug discovery efforts targeting GPCRs. Moreover, also encompassed are the diagnostic, prognostic and therapeutic usefulness of these protein binding domains for GPCR-related diseases.Type: GrantFiled: July 18, 2011Date of Patent: September 27, 2016Assignees: VIB VZW, Vrije Universiteit Brussel, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Jan Steyaert, Els Pardon, Soren Rasmussen, Juan Fung, Brian Kobilka, Toon Laeremans
-
Publication number: 20160060290Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: ApplicationFiled: November 3, 2015Publication date: March 3, 2016Inventors: Samuel Helmer GELLMAN, Pil Seok CHAE, Brian KOBILKA, Soren RASMUSSEN
-
Publication number: 20150368339Abstract: Agents that specifically bind to a muscarinic acetylcholine receptor in a conformationally-specific way can be used to induce a conformational change in the receptor. Such agents have therapeutic applications and can be used in X-ray crystallography studies of the receptor. Such agents can also be used to improve drug discovery via compound screening and/or structure based drug design.Type: ApplicationFiled: February 5, 2014Publication date: December 24, 2015Inventors: Jan Steyaert, Els Pardon, Brian Kobilka, Aaron Ring, Andrew Kruse, Aashish Manglik
-
Publication number: 20150355200Abstract: Provided herein are several methods for selecting agents that bind to transmembrane receptors in a conformationally-selective way. In some embodiments, the method may comprise producing: a transmembrane receptor in an active conformation; and said transmembrane receptor in an inactive conformation and using cell sorting to select, from a population of cells comprising a library of cell surface-tethered extracellular capture agents, cells that are specifically bound to either the transmembrane receptor in its active conformation or the transmembrane receptor in its inactive conformation, but not both. In other embodiments, the method may comprise: contacting a GPCR with a population of cells that comprise a library of surface-tethered extracellular proteins; labeling the cell population with a conformationally-specific binding agent, e.g., a G-protein or mimetic thereof; and using cell sorting to select from the cell population cells that bind to the agent.Type: ApplicationFiled: February 5, 2014Publication date: December 10, 2015Inventors: Aaron Michael Ring, Aashish Manglik, Andrew Kruse, Brian Kobilka
-
Patent number: 9206221Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: GrantFiled: March 14, 2013Date of Patent: December 8, 2015Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen
-
Publication number: 20150210751Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR.Type: ApplicationFiled: April 10, 2015Publication date: July 30, 2015Inventors: Brian Kobilka, Daniel Rosenbaum
-
Patent number: 9045561Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR.Type: GrantFiled: December 18, 2013Date of Patent: June 2, 2015Assignee: THE BOARD OF TRUSTEE OF THE LELAND STANFORD JUNIOR UNIVERSITYInventors: Brian Kobilka, Daniel Rosenbaum
-
Publication number: 20150057166Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising, from N-terminus to C-terminus: a) a first portion of a family C G-protein coupled receptor (GPCR), wherein the first portion comprises the TM1, TM2 and TM3, regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, wherein the second portion comprises the TM4, TM5 TM6 and TM7 regions of the GPCR.Type: ApplicationFiled: October 17, 2014Publication date: February 26, 2015Inventor: BRIAN KOBILKA
-
Patent number: 8889377Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising, from N-terminus to C-terminus: a) a first portion of a family C G-protein coupled receptor (GPCR), wherein the first portion comprises the TM1, TM2 and TM3, regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, wherein the second portion comprises the TM4, TM5 TM6 and TM7 regions of the GPCR.Type: GrantFiled: May 16, 2013Date of Patent: November 18, 2014Assignee: ConfometRx, Inc.Inventor: Brian Kobilka
-
Publication number: 20140275487Abstract: The present invention relates to the field of G protein coupled receptor (GPCR) structural biology and signaling. In particular, the present invention relates to binding domains directed against and/or specifically binding to GPCR:G protein complexes. Also provided are nucleic acid sequences encoding such binding domains and cells expressing or capable of expressing such binding domains. The binding domains of the present invention can be used as universal tools for the structural and functional characterization of G-protein coupled receptors in complex with downstream heterotrimeric G proteins and bound to various natural or synthetic ligands, for investigating the dynamic features of G protein activation, as well as for screening and drug discovery efforts that make use of GPCR:G protein complexes.Type: ApplicationFiled: June 21, 2012Publication date: September 18, 2014Inventors: Jan Steyaert, Els Pardon, Toon Laeremans, Brian Kobilka, Søren Rasmussen, Sébastien Granier, Roger K. Sunahara
-
Publication number: 20140194595Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR.Type: ApplicationFiled: December 18, 2013Publication date: July 10, 2014Inventors: Brian Kobilka, Daniel Rosenbaum
-
Patent number: 8637639Abstract: Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR.Type: GrantFiled: March 30, 2012Date of Patent: January 28, 2014Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Brian Kobilka, Daniel Rosenbaum
-
Publication number: 20130324707Abstract: The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization.Type: ApplicationFiled: August 7, 2013Publication date: December 5, 2013Applicants: The Board of Trustees of the Leland Stanford Junior University, Wisconsin Alumni Research FoundationInventors: Samuel Helmer GELLMAN, Pil Seok CHAE, Brian KOBILKA, Soren RASUMSSEN
-
Publication number: 20130266656Abstract: Bringing membrane proteins into aqueous solution generally requires the use of detergents or other amphiphilic agents. The invention provides a new class of amphiphiles, each of which includes a multi-fused ring system as a lipophilic group. These new amphiphiles confer enhanced stability to a range of membrane proteins in solution relative to conventional detergents, leading to improved structural and functional stability of membrane proteins, including integral membrane proteins. Accordingly, the invention provides new amphiphiles for biochemical manipulations and characterization of membrane proteins. These amphiphiles display favorable behavior with membrane proteins and can be used to aid the solubilization, isolation, purification, stabilization, crystallization, and/or structural determination of membrane proteins.Type: ApplicationFiled: March 14, 2013Publication date: October 10, 2013Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen
-
Patent number: 8530631Abstract: The invention provides amphiphilic compounds and methods for manipulating membrane proteins. Compounds of the invention, for example, the compounds of Formulas I-XIX, can be prepared from readily available starting materials. The amphiphilic compounds can manipulate membrane protein at relatively low concentrations compared to many known detergents. The compounds can be used to aid the isolation of membrane proteins, for example, to aid their solubilization and/or purification. The compounds can also be used to aid the functional and structural determination of membrane proteins, including their stabilization and crystallization.Type: GrantFiled: March 24, 2010Date of Patent: September 10, 2013Assignees: Wisconsin Alumni Research Foundation, The Board of Trustees of the Leland Stanford Junior UniversityInventors: Samuel Helmer Gellman, Pil Seok Chae, Brian Kobilka, Soren Rasmussen