Patents by Inventor Brian L. Webb
Brian L. Webb has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8293484Abstract: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.Type: GrantFiled: September 29, 2010Date of Patent: October 23, 2012Assignee: Corning IncorporatedInventors: Ye Fang, Fang Lai, Laurent A. G. Picard, Brian L. Webb
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Patent number: 8288113Abstract: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.Type: GrantFiled: September 29, 2010Date of Patent: October 16, 2012Assignee: Corning IncorporatedInventors: Ye Fang, Fang Lai, Laurent A. G. Picard, Brian L. Webb
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Publication number: 20120217203Abstract: A dialysis system including a generally sealed vessel configured to receive a buffer, and a dialysis device positioned in the generally sealed vessel. The dialysis device includes an inner member and an outer member trapping a dialysis membrane between the members. The dialysis device is configured to receive a sample to enable dialysis of the sample with respect to the buffer across the membrane.Type: ApplicationFiled: January 6, 2012Publication date: August 30, 2012Applicant: Pierce Biotechnology, Inc.Inventors: Paul Jeffrey Haney, Brian L. Webb, Navid Reza Haghdoost, Rizwan Farooqui, Atul Madhukar Deshpande
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Publication number: 20120088695Abstract: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.Type: ApplicationFiled: September 29, 2010Publication date: April 12, 2012Inventors: Ye Fang, Fang Lai, Laurent A.G. Picard, Brian L. Webb
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Publication number: 20120083428Abstract: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.Type: ApplicationFiled: September 29, 2010Publication date: April 5, 2012Inventors: Ye Fang, Fang Lai, Laurent A.G. Picard, Brian L. Webb
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Patent number: 8129198Abstract: A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.Type: GrantFiled: August 11, 2010Date of Patent: March 6, 2012Assignee: Corning IncorporatedInventors: Ye Fang, Ann M. Ferrie, Yulong Hong, Brian L. Webb
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Patent number: 7939341Abstract: The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.Type: GrantFiled: December 9, 2008Date of Patent: May 10, 2011Assignee: Corning IncorporatedInventors: Alain R.E. Carre, Alexander M. Efremov, Ye Fang, Yulong Hong, Valerie Lacarriere, Joydeep Lahiri, Fang Lai, John C. Mauro, Srikanth Raghavan, Brian L. Webb
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Patent number: 7867700Abstract: A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.Type: GrantFiled: October 11, 2007Date of Patent: January 11, 2011Assignee: Corning IncorporatedInventors: Ye Fang, Ann M. Ferrie, Yulong Hong, Brian L. Webb
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Publication number: 20100323915Abstract: A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.Type: ApplicationFiled: August 11, 2010Publication date: December 23, 2010Inventors: Ye Fang, Ann M. Ferrie, Yulong Hong, Brian L. Webb
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Patent number: 7829290Abstract: Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.Type: GrantFiled: October 27, 2005Date of Patent: November 9, 2010Assignee: Corning IncorporatedInventors: Ye Fang, Fang Lai, Laurent A. G. Picard, Brian L. Webb
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Patent number: 7816096Abstract: Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5?-diphosphate (GDP) salt, and/or an anti-oxidant reagent.Type: GrantFiled: February 1, 2010Date of Patent: October 19, 2010Assignee: Corning IncorporatedInventors: Ye Fang, Ann M. Ferrie, Yulong Hong, Sadashiva K. Pai, Jinlin Peng, Brian L. Webb
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Publication number: 20100152060Abstract: Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5?-diphosphate (GDP) salt, and/or an anti-oxidant reagent.Type: ApplicationFiled: February 1, 2010Publication date: June 17, 2010Inventors: Ye Fang, Ann M. Ferrie, Yulong Hong, Sadashiva K. Pai, Jinlin Peng, Brian L. Webb
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Patent number: 7691580Abstract: Systems, methods and kits that utilize uniquely coded microparticles for performing protein assays are provided. The uniquely coded microparticles are used as a substrate for reverse protein delivery into cells. The microparticles and methods offer the possibility of studying the biological functions of either a single protein of interest in multiple cell types per assay or multiple proteins in a single cell type.Type: GrantFiled: January 29, 2003Date of Patent: April 6, 2010Assignee: Corning IncorporatedInventors: Ye Fang, Brian L. Webb
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Patent number: 7682802Abstract: Buffered assay solutions for performing 1) binding or 2) functional assays on GPCR arrays, along with methods for their use are described. The buffered assay solution has an underlying composition having: a buffer reagent with a pH in the range of about 6.5 to about 7.9; an inorganic salt of either a monovalent or divalent species, at a concentration from about 1 mM to about 500 mM; and optionally a combination of: c) a blocker reagent at a concentration of about 0.01 wt. % to about 2 wt. % of the composition, or d) protease-inhibitor at a concentration of about 0.001 mM to about 100 mM. In an embodiment for functional assay uses, the composition is modified to also include a GTP-analogue, a guanosine 5?-diphosphate (GDP) salt, and/or an anti-oxidant reagent.Type: GrantFiled: December 21, 2005Date of Patent: March 23, 2010Assignee: Corning IncorporatedInventors: Ye Fang, Ann M. Ferrie, Yulong Hong, Sadashiva K. Pai, Jinlin Peng, Brian L. Webb
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Publication number: 20090186776Abstract: A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.Type: ApplicationFiled: June 12, 2008Publication date: July 23, 2009Inventors: Brian L. Webb, Jinlin Peng, Michael D. Brady, Mircea Despa, Keith A. Horn, Joydeep Lahiri, David M. Root, James B. Stamatoff, Po Ki Yuen
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Publication number: 20090093371Abstract: The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.Type: ApplicationFiled: December 9, 2008Publication date: April 9, 2009Inventors: Alain R.E. Carre, Alexander M. Efremov, Ye Fang, Yulong Hong, Valerie Lacarriere, Joydeep Lahiri, Fang Lai, John C. Mauro, Srikanth Raghavan, Brian L. Webb
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Patent number: 7473533Abstract: The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.Type: GrantFiled: December 30, 2004Date of Patent: January 6, 2009Assignee: Corning IncorporatedInventors: Alain R. E. Carre, Alexander M. Efremov, Ye Fang, Yulong Hong, Valerie Lacarriere, Joydeep Lahiri, Fang Lai, John C. Mauro, Srikanth Raghavan, Brian L. Webb
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Publication number: 20080160602Abstract: A biological assay device for use in molecular biology, pharmaceutical research, genomic analysis, combinatorial chemistry, and in the general field of the analysis of molecules that may be deposited on supports of various kinds is provided. Specifically, the present invention includes a fluidic or microfluidic device, which integrates fluidic capability into existing multi-well plates of standard configuration, for performing either single or continuous fluidic manipulations in a high-throughout format. Methods for the use and manufacture of these devices are also provided.Type: ApplicationFiled: December 18, 2007Publication date: July 3, 2008Inventors: Lin He, Jinlin Peng, Youchun Shi, Brian L. Webb, Po Ki Yuen
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Patent number: 7390463Abstract: A biological assay device for use in molecular biology, pharmaceutical research, genomic analysis, combinatorial chemistry, and in the general field of the analysis of molecules that may be deposited on supports of various kinds is provided. Specifically, the present invention includes a fluidic or microfluidic device, which integrates fluidic capability into existing multi-well plates of standard configuration, for performing either single or continuous fluidic manipulations in a high-throughout format. Methods for the use and manufacture of these devices are also provided.Type: GrantFiled: May 24, 2002Date of Patent: June 24, 2008Assignee: Corning IncorporatedInventors: Lin He, Jinlin Peng, Youchun Shi, Brian L. Webb, Po Ki Yuen
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Patent number: 7384779Abstract: A substrate plate or device adapted for use with biological or chemical assays is disclosed. The device may take the form of a multi-well plate having a three-dimensional, porous layer as part of a support surface within each well for immobilizing probe species. The porous layer is characterized as having a plurality of interconnected voids defined by a matrix of contiguous solid material. A method and its variants are also described.Type: GrantFiled: April 12, 2004Date of Patent: June 10, 2008Assignee: Corning IncorporatedInventors: Ye Fang, Ann M. Ferrie, Yulong Hong, Brian L. Webb