Abstract: Disclosed herein are nuclease-based systems for genome editing and methods of using the system for genome editing. Also, disclosed are approaches to enhance Cas9-mediated gene editing efficiency in primary human cells with minimal toxicity when using adeno-associated virus vectors (AAV) to express the guide RNAs necessary for CRISPR/Cas9-based genome editing in the presence of helper proteins.

Abstract: Reconfigurable microfluidic systems are based on networks of microfluidic cavities connected by hydrophobic microfluidic channels. Each cavity is classified as either a reservoir or a node, and includes a pressure port via which gas pressure may be applied. Sequences of gas pressures, applied to reservoirs and nodes according to a fluid transfer rule, enable fluid to be moved from any reservoir to any other reservoir in a system. Such systems are suitable for automated, multi-input, multi-output homogeneous assays.

Abstract: Reconfigurable microfluidic systems are based on networks of microfluidic cavities connected by hydrophobic microfluidic channels. Each cavity is classified as either a reservoir or a node, and includes a pressure port via which gas pressure may be applied. Sequences of gas pressures, applied to reservoirs and nodes according to a fluid transfer rule, enable fluid to be moved from any reservoir to any other reservoir in a system. Such systems are suitable for automated microwell plate interfaces.

Abstract: Embodiments of a central emulator, distributed emulator and method for emulation of a system are generally described herein. The central emulator may receive state variables from distributed emulators at various emulation times. As an example, such an emulation time may be related to an emulation event at one of the distributed emulators. The central emulator may determine global snapshots of the system emulation for the emulation times based on the state variables. The global snapshots may be used to control a timing of the system emulation for operations such as rewinding, pausing, forwarding and/or setting to a target time.

Abstract: The described embodiments transfer an activity from a source electronic device to a companion electronic device. The source electronic device receives activity information describing an activity performed in a first application at the source electronic device, determines an activity identifier for the activity information, and broadcasts an activity advertisement comprising the activity identifier. Upon receiving the activity advertisement, the companion electronic device determines whether a second application that is associated with the first application is available at the companion electronic device. Then, the companion electronic device configures the second application and commences performing the activity with the second application at the companion electronic device.

Abstract: Disclosed are fluidics devices and assemblies allowing for fluid flow between a plurality of wells. The fluidics devices and assemblies that are provided mimic in vivo tissue environments by allowing for initially segregated tissue cultures that can then be linked through fluid flow to measure integrated tissue response. The fluidics devices and assemblies provide a pumpless system using surface tension, gravity, and channel geometries. By linking human tissue functional systems to better simulate in vivo feedback and response signals between the tissues, the need for testing in animals can be minimized.

Abstract: The present invention provides nucleic acids, vectors, host cells, methods and compositions to confer and/or augment immune responses mediated by cellular immunotherapy, such as by adoptively transferring CD8+ central memory T cells or combinations of central memory T cells with CD4+ T cells that are genetically modified to express a chimeric receptor. In some alternatives the genetically modified host cell comprises a nucleic acid comprising a polynucleotide coding for a ligand binding domain, a poly nucleotide comprising a customized spacer region, a polynucleotide comprising a transmembrane domain, and a polynucleotide comprising an intracellular signaling domain. In some alternatives, the ligand binding domains binds to CD171.

Abstract: A CD19-OR-CD20 chimeric antigen receptor (CAR) protein construct is provided. Also provided are nucleic acids encoding the CD19-OR-CD20 CAR; and methods of use, e.g. in the treatment of B cell malignancies. The CD19-OR-CD20 CAR of the invention is a bispecific CAR that can trigger T-cell activation upon detection of either CD19 or CD20 (or both). It is a single molecule that confers two-input recognition capability upon human T cells engineered to stably express this CAR.

Abstract: Methods and apparatuses for wireless communication devices to discover each other and share information are described. Wireless communication devices transmit and receive messages to identify compatible wireless communication devices. The messages include indicators for a communication service and one or more user identification values. A scanning wireless communication device identifies a message from a broadcasting wireless communication device that satisfies a set of matching filter criteria and extracts user identification values from the message. The scanning wireless communication device compares the extracted user identification values to a local set of user identification values. When an extracted user identification value from the message matches a user identification value in the local set of user identification values, the scanning wireless communication device establishes a connection to the broadcasting wireless communication device according to the communication service indicated in the message.

Abstract: An approach is provided for providing adaptable schema based payloads for state advertisement messages. In some implementations, multiple schemas (e.g., payload data and structure definition) can be used to flexibly and dynamically define advertisement message payloads. For example, the computing device can use advertising message to share and broadcast information between devices by using adaptable schema based payloads. The computing device can store schemas, receive service advertising data, and determine a schema for encoding service advertising data into an advertising message. The computing device can generate the advertising message, encode a schema identifier into the advertising message header, and encode device state data into the advertising message payload according to the determined schema.

Abstract: An approach is provided for providing adaptable schema based payloads for state advertisement messages. In some implementations, multiple schemas (e.g., payload data and structure definition) can be used to flexibly and dynamically define advertisement message payloads. For example, the computing device can use advertising message to share and broadcast information between devices by using adaptable schema based payloads. The computing device can store schemas, receive service advertising data, and determine a schema for encoding service advertising data into an advertising message. The computing device can generate the advertising message, encode a schema identifier into the advertising message header, and encode device state data into the advertising message payload according to the determined schema.

Abstract: An approach is provided for providing adaptable schema based payloads for state advertisement messages. In some implementations, multiple schemas (e.g., payload data and structure definition) can be used to flexibly and dynamically define advertisement message payloads. For example, the computing device can use advertising message to share and broadcast information between devices by using adaptable schema based payloads. The computing device can store schemas, receive service advertising data, and determine a schema for encoding service advertising data into an advertising message. The computing device can generate the advertising message, encode a schema identifier into the advertising message header, and encode device state data into the advertising message payload according to the determined schema.

Abstract: Disclosed are fluidics devices and assemblies allowing for fluid flow between a plurality of wells. The fluidics devices and assemblies that are provided mimic in vivo tissue environments by allowing for initially segregated tissue cultures that can then be linked through fluid flow to measure integrated tissue response. The fluidics devices and assemblies provide a pumpless system using surface tension, gravity, and channel geometries. By linking human tissue functional systems to better simulate in vivo feedback and response signals between the tissues, the need for testing in animals can be minimized.

Abstract: Described is a personal device and methods for measuring the concentration of an analyte in a sample of gas. The device and method may utilize a chemically selective sensor element with low power consumption integrated with circuitry that enables wireless communication between the sensor and any suitable personal electronic device such as a smartphone, tablet, or computer. In preferred form, the sensor circuitry relies upon the quantum capacitance effect of graphene as a transduction mechanism. Also in preferred form, the device and method employ the functionalization of the graphene-based sensor to determine the concentration of an analyte in exhaled breath.

Abstract: The present disclosure relates to method, system for microfluidic control. One or more embodiments of the disclosure relate to pneumatically actuated “lifting gate” microvalves and pumps. In some embodiments, a microfluidic control module is provided, which comprises a plurality of pneumatic channels and a plurality of lifting gate valves configured to be detachably affixed to a substrate. The plurality of lifting gate valves are aligned with at least one fluidic channel on the substrate when affixed to the substrate. Each of the valves comprises: a pneumatic layer, a fluidic layer, and a pneumatic displacement chamber between the pneumatic layer and the fluidic layer. The fluidic layer has a first side facing the pneumatic layer and a second side facing away from the pneumatic layer, wherein the second side has a protruding gate configured to obstruct a flow of the fluidic channel when the fluidic layer is at a resting state.

Abstract: A self-cleaning floor assembly is configured to form or be positioned on a floor of an enclosed space. The self-cleaning floor assembly includes a moveable floor including a moveable floor panel, an actuation system that is operatively coupled to the floor panel, and a cleaning system proximate to at least a portion of the moveable floor. The actuation system is configured to move at least a portion of the floor panel into and through the cleaning system during a cleaning cycle. The cleaning system is configured to clean the portion(s) of the floor panel during the cleaning cycle.

Abstract: Method, composition, kit and system for isolating amplifiable nucleic acid from specimens preserved in a liquid-based cytology preservative that contains formaldehyde. The technique relies on the use of 2-imidazolidone and a protease enzyme, such as proteinase K, at elevated temperatures. Advantageously, RNA can be isolated and used as a template in nucleic acid amplification reactions.

Abstract: The present invention provides genetic tags operably linked to transgenes. The expression of the genetic tag allows identification, detection, selection, and ablation of cells expressing the transgene and the genetic tag. In some alternatives the genetically modified host cell comprises a transgene comprising a polynucleotide coding for a chimeric antigen receptor comprising a ligand binding domain, a polynucleotide comprising a spacer region, a polynucleotide comprising a transmembrane domain, and a polynucleotide comprising an intracellular signaling domain and a polynucleotide coding for a genetic tag.

Abstract: Reconfigurable microfluidic systems are based on networks of microfluidic cavities connected by hydrophobic microfluidic channels. Each cavity is classified as either a reservoir or a node, and includes a pressure port via which gas pressure may be applied. Sequences of gas pressures, applied to reservoirs and nodes according to a fluid transfer rule, enable fluid to be moved from any reservoir to any other reservoir in a system. Systems may be configured with multiple switched interaction regions connected in series for scalable, multiplexed immunoassays. Multiple, switched interaction regions may also be implemented with microvalves.

Abstract: The present invention relates to chimeric transmembrane immunoreceptors, named “zetakines,” comprised of an extracellular domain comprising a soluble receptor ligand linked to a support region capable of tethering the extracellular domain to a cell surface, a transmembrane region and an intracellular signalling domain. Zetakines, when expressed on the surface of T lymphocytes, direct T cell activity to those specific cells expressing a receptor for which the soluble receptor ligand is specific. Zetakine chimeric immunoreceptors represent a novel extension of antibody-based immunoreceptors for redirecting the antigen specificity of T cells, with application to treatment of a variety of cancers, particularly via the autocrin/paracrine cytokine systems utilized by human maligancy.