Abstract: The present disclosure includes compounds, compositions, and methods for nucleic acid amplification reactions. In particular, the present disclosure provides compounds, compositions, and methods for viability PCR (vPCR) applications, in which the compounds that selectively bind to nucleic acids from non-viable cells.

Abstract: Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.

Abstract: The present invention discloses a method for realizing multi-column continuous chromatography design and analysis based on a chromatography model, and a method for realizing multi-column continuous chromatography design and analysis based on an artificial neural network.

Abstract: A vehicle window glass is suitable for cooperating with a projection device to provide a projection function. The vehicle window glass includes a first glass body, a second glass body, an imaging layer and a dimming film sandwiched between the first glass body and the second glass body, wherein an image projected by the projection device on one side of the vehicle window glass displays the projected image on the other side via the vehicle window glass, and the dimming film is configured to be capable of adjusting light transmittance and/or haze to enhance the projection effect of the imaging layer and/or provide privacy effect.

Abstract: The present invention discloses a water jet trajectory presetting method. The method includes following steps: setting a telescoping direction of a water jet and a telescoping direction of an ultrasonic probe in parallel; identifying a water-jetting point in a sagittal ultrasonic image, determining a first position of a cutter hole in telescoping direction; controlling ultrasonic probe to move to obtain a cross-sectional ultrasonic image of the first position; identifying the water jetting point and a direction of a water column in cross-sectional ultrasonic image, controlling water jet to rotate until water column points to ultrasonic probe, generating a water jet trajectory starting point at the position of water-jetting point on sagittal ultrasonic image; determining a second position set in the telescoping direction of water jet as a water jet trajectory end point, generating on ultrasonic image a mark line from starting point to end point.

Abstract: A presentation control method for in-vehicle glass of a vehicle, includes obtaining a physical parameter associated with the vehicle; and determining content presented on the in-vehicle glass according to the physical parameter. Further, the present disclosure relates to a presentation control device for in-vehicle glass of a vehicle, wherein the presentation control device includes: a processor; and a memory for storing computer executable instructions that, when executed, cause the processor to execute the presentation control method according to the presentation control method.

Abstract: A laminated glass, includes a glass plate, a polymer layer, and a functional device layer, wherein, the polymer layer is located between the glass plate and the functional device layer; the laminated glass further optionally includes another polymer layer, which is located at the other side of the functional device layer, opposite to the glass plate; the polymer layers each independently include polymer, which is selected from crosslinked ethylene-vinyl acetate copolymer, polyolefin thermoplastic elastomer, and thermoplastic polyurethane elastomer.

Abstract: Disclosed in the application is a waterjet trajectory presetting apparatus, which is used for implementing a waterjet trajectory presetting method.

Abstract: The present invention provides an automatic water jet cutter system, where a continuous boundary position trajectory pre-planned on a navigation image is fitted to generate a motion control position trajectory; a water jet cutter coordinate system is established, the motion control position trajectory is converted into the water jet cutter coordinate system, then motion position trajectory parameters for each axis are calculated; according to the motion position trajectory parameters for each axis, the water jet cutter is controlled to perform respective motions in a jet flow axis, a suction flow axis, a linear motion axis and a rotary motion axis by a multi-axis linkage control method; and in the multi-axis linkage control method, an error in any one of the jet flow axis, the suction flow axis, the linear motion axis and the rotary motion axis of the water jet cutter are correlated with errors in the other three axes.

Abstract: The present application discloses a biplanar ultrasound image planning method, which solves the problem of large path planning errors.

Abstract: A glass assembly includes a glass body having a first surface and a second surface which are oppositely arranged, wherein at least a portion of incident light entering the glass body is totally reflected between the first surface and the second surface; a light guiding layer arranged on the first surface or the second surface for guiding the incident light out of the second surface; an anti-reflection layer attached to the second surface to reduce the reflection of the light incident on the second surface from outside of the glass body. According to the glass assembly integrated with the anti-reflection layer, the light pollution reflected by the surface of the glass assembly can be particularly reduced on the premise of not affecting the performance and aesthetics of the glass, and the user experience can be improved.

Abstract: A multi-image information fusion method for tissue cutting path planning, comprising: acquiring position information of a target tissue working area calibrated in an endoscopic image, and converting the position information of the target tissue working area into coordinate information in a standard coordinate system, so as to obtain an endoscopic image in the standard coordinate system; acquiring a three-dimensional ultrasound image of a target tissue; and extracting two-dimensional slice image contour position information of the three-dimensional ultrasound image, and converting the two-dimensional slice image contour position information into coordinate information in the standard coordinate system, so as to obtain an ultrasound image in the standard coordinate system.

Abstract: Provided herein are systems, methods, and compositions for bioluminescence-triggered catalysis of bioorthogonal labeling chemistries in a proximity dependent manner. In particular, provided herein are bioluminescent proteins or complexes, luminophore substrates thereof, photocatalysts or photosensitizers, activatable labels, and systems thereof, and methods of catalytically activating the activatable labels via bioluminescence-triggered catalysis.

Abstract: A laminated glass, includes a glass plate, a polymer layer, and a functional device layer, wherein, the polymer layer is located between the glass plate and the functional device layer; the laminated glass further optionally includes another polymer layer, which is located at the other side of the functional device layer, opposite to the glass plate; the polymer layers each independently include polymer, which is selected from crosslinked ethylene-vinyl acetate copolymer, polyolefin thermoplastic elastomer, and thermoplastic polyurethane elastomer.

Abstract: An optimization method for capturing proteins by multi-column continuous chromatography (MCC), including the following steps: step 1, under the conditions of a set loading protein concentration and an arbitrary load residence time, performing a single time of protein breakthrough experiment to obtain a protein breakthrough curve; step 2, under a set breakthrough percentage for a target protein, integrating the breakthrough curve to obtain a single-column loading capacity and establishing a linear relationship between the interconnected load time and the load residence time; step 3, solving for the optimal number of operating columns for capturing proteins by MCC based on step 2; step 4, solving for the optimal load residence time for capturing proteins by MCC based on step 2, step 3; and step 5, solving for the maximum productivity of capturing proteins by MCC based on step 4.

Abstract: Provided herein are surfactant compounds that can be used to aid in preparation of protein samples for analysis, for example by mass spectrometry.

Abstract: Provided herein are systems and methods for characterizing target/ligand engagement. In particular, luciferase-labeled polypeptide targets are used to detect or quantify target/ligand engagement (e.g., within a cell or cell lysate).

Abstract: Provided herein are enhanced luciferase enzymes for use with thermostable luciferin analogs for bioluminescent assays. In particular, the present disclosure provides compositions, assays, and methods for performing a bioluminescent assay using enhanced, high-activity luciferase enzymes compatible with thermostable luciferins, such as 5,5-disubstituted luciferin analogs.

Abstract: A coating for a glass and a forming method thereof, and an automotive window are provided. The coating includes: a first material layer which has a first elastic modulus; and a second material layer which includes at least one material layer, wherein the second material layer has a first surface and a second surface opposite to the first surface, the first surface is used for disposing the first material layer, the second surface is adaptable for facing a surface of the glass when the coating is applied on the glass, and the second material layer has a second elastic modulus less than the first elastic modulus. Anti-scratch ability of the coating and scratch resistance of the automotive window may be improved, which avoids scratches on the automotive window.

Abstract: Provided herein are compounds, compositions, systems, and methods for photoactivatable labeling, which can be actuated within biological systems. In particular, compounds disclosed herein include vinyl-extended-aryl azide moieties that undergo photoactivation to generate reactive intermediates, which can form covalent linkages with biomolecules. The photoactivation can be conducted by a variety of mechanisms including ultraviolet (UV) irradiation, visible light irradiation, or energy transfer (e.g., from a photocatalyst). The compounds also include functional moieties that provide useful functionalities, for example detection of biomolecules, such as fluorophores, capture elements (e.g., biotin), or reactive moieties (e.g., click handles) and bifunctional moieties comprising a bioactive compound and a detection/capture element.