Abstract: A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules.

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.

Abstract: A video signal conversion method includes: receiving an input signal from a video source; extracting an image metadata from the input signal; determining whether the input signal corresponds to a high dynamic range (HDR) imaging format according to at least one format information of the image metadata and determining whether a video receiver supports the high dynamic range imaging format; in response to the input signal corresponding to the high dynamic range imaging format and the video receiver being not support the high dynamic range imaging format, generating a conversion command; receiving, by a video processor, the conversion command; converting, by the video processor, the input signal into an output signal corresponding to a standard dynamic range (SDR) imaging format according to the conversion command; sending, by the video processor, the output signal to the video receiver; and receiving by the video receiver, the output signal in SDR imaging format.

Abstract: A method for synthesizing a nucleic acid includes providing an initiator having an unprotected nucleoside base and a 3? hydroxyl group at a 3? terminus, providing a nucleic acid polymerase having at least one conservative catalytic polymerase domain of a family-B DNA polymerase, providing at least one nucleotide monomer, and exposing the initiator to the nucleotide monomer in the presence of the nucleic acid polymerase, at a temperature ranging from 20° C. to 90°, and in the absence of a template, such that the nucleotide monomer is incorporated to the initiator to form the nucleic acid. Also disclosed is a kit includes the initiator, the nucleic acid polymerase, and the nucleotide monomer, and is used according to the method.

Abstract: A detection system and a detection method are provided. The detection system includes at least one illumination device and a host. The illumination device has a radar to obtain spatial information of a position of the illumination device. The host is connected to the at least one illumination device. The host receives the spatial information from the illumination device and transmits a change of the spatial information to an emergency system.

Abstract: A video signal conversion device includes a front end interface, a rear end interface, a video detector and a video processor. The front end interface is coupled to a video source to receive an input signal. The rear end interface is coupled to a video receiver. The video detector determines whether the input signal corresponds to HDR imaging format. The video detector generates a conversion command in response to the input signal corresponding to HDR imaging format. The video processor is coupled to the front end interface, the rear end interface and the video detector. When the video processor receives the conversion command, the video processor converts the input signal into an output signal with SDR imaging format. The video processor sends the output signal to the video receiver via the rear end interface.

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.

Abstract: A diffusive gradients in thin films (DGT) test device for lake water, including: a floating ball and a plurality of water sampling units. A DGT detection device, a water quality detection device with a multi-parameter probe, and a water sampling device are disposed in each of the water sampling units. The water sampling device includes: a plurality of sampling bottles, each of the sampling bottles is provided with an inlet pipe and an exhaust pipe, each of the inlet pipes and exhaust pipes is provided with a one-way solenoid valve; a peristaltic pump, a water sample outlet of the peristaltic pump communicates with the inlet pipes of the plurality of sampling bottles via a multi-way connector; the exhaust pipes of the plurality of sampling bottles are connected to an exhaust manifold via a multi-way connector; a control device connected with the peristaltic pump and each of the one-way solenoid valves.

Abstract: The present disclosure provides a diffusive gradients in thin films (DGT) probe test device for a sediment core in a lake, including: a sampling tube, where, a settlement limit device is disposed on an outer wall of the sampling tube and a DGT probe and a multi-parameter water-quality detection electrode are installed within the sampling tube; a movable mudguard device comprising a connecting rod and a mudguard, where, the connecting rod rotates around a rotating shaft to drive the mudguard to move from a position where an opening at the lower end of the sampling tube is sealed to a side of the sampling tube; a position-limit mechanism removably installed outside the sampling tube; a hammering device located above the sampling tube and fixedly connected to the sampling tube; and a floating ball located above the hammering device and connected to the hammering device via a first pull rope.

Abstract: A method for synthesizing a nucleic acid includes providing an initiator having a 3? end having an unprotected hydroxyl group, providing a nucleic acid polymerase having at least a conservative catalytic polymerase domain of a family-B DNA polymerase, providing a nucleotide monomer, and exposing the initiator to the nucleotide monomer in the presence of the nucleic acid polymerase and a metal cofactor which is a bivalent cation, and in the absence of a template, such that the nucleotide monomer is incorporated to the initiator. The kit includes the initiator, the nucleic acid polymerase, and the nucleotide monomer, and is used according to the method.

Abstract: A pressure sensor package includes a package lead frame including a molding plastic layer with a top surface and a plurality of lead frame units mounted in the molding plastic layer, a sidewall disposed on the top surface of the molding plastic layer and surrounding a receiving chamber, a pressure sensor module mounted on the top surface of the molding plastic layer and disposed in the receiving chamber, and a packaging silicone mounted in the receiving chamber to encapsulate the pressure sensor module.

Abstract: This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.

Abstract: A system including a network communication device, a storage device, and a controller is provided. The storage device stores first mappings between IP addresses and devices, and second mappings between software and devices. The controller obtains a connection log from the proxy server or the firewall device via the network communication device, uses the first mappings and the second mappings to analyze the connection log to determine one or more different connections between connections of devices on which first software is installed and connections of devices on which the first software is not installed, determines whether the first software functions normally on a first device blocking the different connections, and adds destination addresses of the different connections into a blocking list in response to the first software functioning normally on the first device, such that the proxy server or the firewall device blocks all connections towards the destination addresses.

Abstract: Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule.

Abstract: A video signal conversion device includes a frontend interface circuit, a FPGA video processor and a backend interface circuit. The frontend interface circuit receives a HDR video input signal from a video transmitting device. The FPGA video processor outputs a SDR first video output signal. A video receiving device receives the first video output signal and a HDR second video output signal from the FPGA video processor through the video bridge controller of the backend interface circuit by PCI-E.

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.

Abstract: A video signal conversion device includes a frontend interface circuit, a FPGA video processor and a backend interface circuit. The frontend interface circuit receives a HDR video input signal from a video transmitting device. The FPGA video processor outputs a SDR first video output signal. A video receiving device receives the first video output signal and a HDR second video output signal from the FPGA video processor through the video bridge controller of the backend interface circuit by PCI-E.

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3? sugar hydroxyl, as well as methods and kits using the same.

Abstract: This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.