Abstract: The disclosure provides methods for treating a subject in need thereof comprising administering to the subject a therapeutically-effective dose of psilocybin. The methods described herein may be used to treat a variety of diseases, disorders, and conditions. For example, the methods may be used to treat neurocognitive disorders (e.g., Alzheimer's disease, Parkinson's disease), ADHD, Epilepsy, Autism, Sleep-wake disorders, Chronic pain, Inflammatory Disorders, IBD, Stroke, ALS, and/or Multiple Sclerosis.

Abstract: One aspect of the present invention relates to double-stranded RNA (dsRNA) agent capable of inhibiting the expression of a target gene. The antisense strand of the dsRNA molecule comprises at least one thermally destabilizing nucleotide occurring at a seed region; the dsRNA comprises at least four 2?-fluoro modifications, and the sense strand of the dsRNA molecule comprises ligand, wherein the ligand is an ASGPR ligand. Other aspects of the invention relate to pharmaceutical compositions comprising these dsRNA molecules suitable for therapeutic use, and methods of inhibiting the expression of a target gene by administering these dsRNA molecules, e.g., for the treatment of various disease conditions.

Abstract: The present disclosure provides systems and methods for transposing a cargo nucleotide sequence into a target nucleic acid site in a target nucleic acid. These systems and methods may comprise a double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a recombinase complex, an effector complex comprising an effector and at least one engineered guide polynucleotide configured to hybridize to the target nucleic acid, and the recombinase complex wherein the recombinase complex is configured to recruit the cargo nucleotide to the target nucleic acid site.

Abstract: The present disclosure provides systems and methods for transposing a cargo nucleotide sequence into a target nucleic acid site in a target nucleic acid. These systems and methods may comprise a double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a recombinase or transposase complex, an effector complex comprising an effector and at least one engineered guide polynucleotide configured to hybridize to the target nucleic acid, and the recombinase or transposase complex wherein said recombinase or transposase complex is configured to recruit the cargo nucleotide to the target nucleic acid site.

Abstract: The present disclosure provides systems and methods for transposing a cargo nucleotide sequence into a target nucleic acid site in a target nucleic acid. These systems and methods may comprise a double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a recombinase or transposase complex, an effector complex comprising an effector and at least one engineered guide polynucleotide configured to hybridize to the target nucleic acid, and the recombinase or transposase complex wherein said recombinase or transposase complex is configured to recruit the cargo nucleotide to the target nucleic acid site.

Abstract: The present disclosure provides systems and methods for transposing a cargo nucleotide sequence into a target nucleic acid site. These systems and methods may comprise a double-stranded nucleic acid comprising the cargo nucleotide sequence, wherein the cargo nucleotide sequence is configured to interact with a recombinase complex, an effector complex comprising an effector and at least one engineered guide polynucleotide configured to hybridize to the target nucleic acid site, and the recombinase complex wherein said recombinase complex is configured to recruit the cargo nucleotide to the target nucleic acid site.

Abstract: This invention relates to the large-scale production of psilocybin for use in medicine. More particularly, it relates to a method of obtaining high purity crystalline psilocybin, particularly, in the form of Polymorph A. It further relates to a method for the manufacture of psilocybin and intermediates in the production thereof and formulations containing psilocybin.

Abstract: Aspects of the present disclosure relate to an electric or hybrid vehicle. Aspects of the vehicle may be used to provide a variety of functionality, including power exporting and aggregation, power take off functionality, thermal signature reduction, and improved vehicle longevity, vehicle turning, and vehicle control techniques.

Abstract: The present disclosure provides for endonuclease enzymes having distinguishing domain features, as well as methods of using such enzymes or variants thereof.

Abstract: This invention relates to the large-scale production of psilocybin for use in medicine. More particularly, it relates to a method of obtaining high purity crystalline psilocybin, particularly, in the form of Polymorph A. It further relates to a method for the manufacture of psilocybin and intermediates in the production thereof and formulations containing psilocybin.

Abstract: A process to partition a video feed to segment live player activity includes receiving, on a first recurring basis, a transmission of a central video feed from a first camera. The central video feed is calibrated against a spatial region represented in at least two dimensions that is encompassed by the central video feed. The process includes receiving, on a second recurring basis, a respective time-stamped position information from each tracking device in a plurality of tracking devices. Each tracking device is worn by a corresponding subject on the spatial region and transmits positional information that describes a time-stamped position of the corresponding subject in the spatial region. The process uses the received information and the calibration to define a first sub-view of the central video feed associated with a first subject. The first sub-view comprises a corresponding sub-frame associated with the first subject.

Abstract: The invention relates to a method for identifying parking spaces, including emitting an ultrasonic signal and receiving a reflected signal; and providing a grid which of a region having a plurality of cells. Each cell is assigned to a partial region of the region surrounding the vehicle and an occupancy value is assigned to each cell. The reflected signal is assigned to a set of cells of the grid based on a transit time of the ultrasonic signal between emission and reception. The occupancy values of the cells to which the reflected signal is allocated are increased by a specific value. An occupancy information item is determined based on the occupancy values of the cells. The signal emitting/receiving and the reflected signal assigning are repeated multiple times. Parking space identification is performed based on an occupancy status of the cells specified using the occupancy information item determination.

Abstract: Described herein are methods, compositions, and systems for multiplex editing using Cas enzymes or editing of T-cells or related cells using Cas enzymes.

Abstract: Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.

Abstract: The invention relates to RNAi agents, e.g., double stranded RNAi agents, targeting the Serpina1 gene, and methods of using such RNAi agents to inhibit expression of Serpina1 and methods of treating subjects having a Serpina1 associated disease, such as a liver disorder.

Abstract: In an embodiment, a process to predict a probability of a future event occurring in a present competition includes receiving time-stamped position information of one or more participants in the present competition. The time-stamped position information is captured by a telemetry tracking system during the present competition. The process uses the time-stamped position information to determine a first play situation of the present competition. The process determines, based on at least the first play situation and playing data associated with at least a subset of one or both of a first set of one or more participants and a second set of one or more participants, a prediction of the probability of a first future event occurring at the present competition.

Abstract: Disclosed herein are engineered nucleases and nuclease systems, including chimeric nucleases and chimeric nuclease systems. Engineered and chimeric nucleases disclosed herein include nucleic acid guided nuclease. Additionally disclosed herein are methods of generating engineered nucleases and methods of using the same.

Abstract: Described herein are methods, compositions, and systems derived from uncultivated microorganisms useful for gene editing.

Abstract: The present disclosure provides for endonuclease enzymes as well as methods of using such enzymes or variants thereof.

Abstract: A method for characterizing an object in the surroundings of a motor vehicle in which method the motor vehicle is moved relative to the object and ultrasonic signals are emitted with an ultrasonic sensor. Echoes of the ultrasonic signals reflected by the object are received, respective amplitudes of the received echoes are determined, and a classification of a height of the object is established based on the amplitudes.