Abstract: A hand held vacuum using fluid, such as steam, to enhance cleaning is provided. The hand held vacuum includes a main body housing having a vacuum inlet and at least one fluid discharge aperture. The vacuum inlet cooperates with a vacuum fan and the fluid discharge aperture cooperates with a pump mechanism. A fluid supply tank is adapted to be removably connected to the main body housing and has a fill opening. A closure is removably attached to the fluid supply tank and is adapted to seal the fill opening and provide a discharge flow path. A flow path extension provides fluid communication between a low point in the fluid supply tank and the discharge flow path of the closure. The flow path extension may be rigid. The discharge flow path of the closure may extend between a radial inlet and an axial outlet of the closure.

Abstract: A selective targeting method is disclosed which comprises contacting a peptide library with an anti-target to allow the peptides to bind; separating non-binding peptides from the anti-target bound peptides, contacting the non-binding anti-target peptides with a target allowing the unbound peptides to bind with the target to form a target-bound peptide complex; separating the target-bound peptide complex from peptides which do not bind to the target, and identifying the target-bound peptides. In one embodiment the target is skin or hair. In another embodiment the anti-target is hair when the target is skin, and the anti-target is skin when the target is hair.

Abstract: The present invention provides targeted enzymes that bind to targets better than the corresponding pre-targeted enzymes bind the target under like conditions, methods of making targeted enzymes, methods of using targeted enzymes to treat diseases, and pharmaceutical compositions comprising targeted enzymes.

Abstract: The present application relates to peptides which bind to a target stain, phenol oxidizing enzyme-binding peptide complexes wherein the binding peptide is attached to the C-terminus of the phenol oxidizing enzyme or is inserted or substituted into the phenol oxidizing enzyme. In a preferred embodiment the phenol oxidizing enzyme is a laccase specifically Stachybotrys oxidase B and variants thereof. The invention provides expression vectors comprising the phenol oxidizing enzyme-binding peptide complex as well as host cells comprising the vectors.

Abstract: The present invention provides targeted enzymes that bind to targets better than the corresponding pre-targeted enzymes bind the target under like conditions, methods of making targeted enzymes, methods of using targeted enzymes to treat diseases, and pharmaceutical compositions comprising targeted enzymes.

Abstract: The present invention provides methods and compositions relating to polypeptides engineered to bind a target and have at least two functional domains.

Abstract: A selective targeting method is disclosed comprising contacting a library of ligands, particularly a peptide library, with an anti-target to allow the ligands to bind to the anti-target; separating the non-binding ligands from the anti-target bound ligands, contacting the non-binding anti-target ligands with a target allowing the unbound ligands to bind with the target to form a target-bound ligand complex; separating the target-bound ligand complex from ligands which do not bind to the target, and identifying the target-bound ligands on the target-bound ligand complex wherein the target-bound ligands have a KD in the range of about 10−7 to 10−10 M. Additionally claimed are the ligands identified according to the method.