Abstract: A process for purification of recombinant porphobilinogen deaminase (rhPBGD) on an industrial scale by starting from a rhPBGD containing extract obtained from a fermentation of a recombinant cell capable of expressing the rhPBGD and the use of the purified product for the preparation of a medicament.

Abstract: A cutting insert having a front cutting edge and a connecting surface including male- and/or female-like engagement portions, in order to rigidly secure the cutting insert, a geometrically predetermined reference locus serving as a force-application spot for the clamping of the cutting insert. Simultaneously active engagement portions are of only two types—a first, elongate engagement portion, which is located between the front cutting edge and the reference locus, and a second, elongate engagement means, which is oriented at an angle to the first engagement portion and situated at a greater distance from the front cutting edge than the reference locus. In such a way, it is guaranteed that a tightening force always is applied to the cutting insert in a substantially triangular area, which is defined by the free ends of the engagement portions. Furthermore, a tool for chip removing machining, as well as an attachment, e.g., a shim plate, for such tools is provided.

Abstract: A double ended metal-cutting insert includes a pair of circular cutting edges disposed at respective end surfaces of the insert. Each end surface includes an upper portion connected to the cutting edge, and a lower portion connected to the upper portion by a concave transition portion. The lower portion is recessed relative to the upper portion in a direction toward the opposite cutting edge. The lower portion is a flat face oriented perpendicular to the bottom surface.

Abstract: A parting tool includes a thin holder forming a groove open at one end, and a double-ended cutting insert mounted in the groove. The insert includes front and rear cutting edges and a rear end surface abutting against a rear support surface of the groove. The rear support surface is configured to enable differently shaped double-ended inserts to be mounted therein without the occurrence of contact between the rear cutting edge and a surface of the holder.

Abstract: A cutting insert for grooving and parting as well as profiling and longitudinal turning of metallic work-pieces, includes a shaft part and at least one cutting head. There is a front end surface at the front end of the cutting insert, and a rear end surface at the rear end of the cutting insert. The shaft part includes a top side, a bottom side and side surfaces extending between the tope and bottom two sides. The cutting head carries a cutting edge defined by the intersection between a chip surface and a clearance surface. The cutting edge has a circular shape and has mainly a constant curvature between the ends thereof. The chip side of the cutting head is provided with a first chip-forming device placed immediately inside the cutting edge.

Abstract: The present invention relates to a cell capable of producing recombinant human LAMAN (rhLAMAN), said cell comprising the 3066 basepair EcoRI-XbaI fragment of a human cDNA which codes for a human LAMAN protein in which the position corresponding to position 186 of the full length hLAMAN protein is Aspartic acid (Asp, D). In particular, the invention relates to a cell comprising a DNA sequence which codes for the amino acid sequence shown in SEQ ID NO 12. One embodiment is an expression plasmid pLamanExp1 having the sequence shown in SEQ ID NO. 2.

Abstract: A process for purification of recombinant porphobilinogen deaminase (rhPBGD) on an industrial scale by starting from a rhPBGD containing extract obtained from a fermentation of a recombinant cell capable of expressing the rhPBGD and the use of the purified product for the preparation of a medicament.

Abstract: A cutting insert for grooving and parting as well as profiling and longitudinal turning of metallic work-pieces, includes a shaft part and at least one cutting head. There is a front end surface at the front end of the cutting insert, and a rear end surface at the rear end of the cutting insert. The shaft part includes a top side, a bottom side and side surfaces extending between the tope and bottom two sides. The cutting head carries a cutting edge defined by the intersection between a chip surface and a clearance surface. The cutting edge has a circular shape and has mainly a constant curvature between the ends thereof. The chip side of the cutting head is provided with a first chip-forming device placed immediately inside the cutting edge.

Abstract: A tool for chip removing machining includes a holder and a cutting insert removably mounted therein. The insert includes a shaft having opposite ends and defining a first longitudinal center line. A cutting head projects integrally from at least one end of the shaft. The cutting head includes a cutting edge forming an angle of 45° with the first longitudinal center line. Alternatively, or additionally, the cutting head defines a second longitudinal center line forming an angle of 45° with the first longitudinal center line.

Abstract: A cutting insert for grooving operations includes a central shank portion and a forward cutting head. The cutting head includes a front clearance surface having a main cutting edge at an upper end thereof. The clearance surface is non-planar. Rather, the clearance surface exhibits a curvature in order to strengthen the main cutting edge.

Abstract: A tool for chip removing machining includes a holder and a cutting insert removably mounted therein. The insert includes a shaft having opposite ends and defining a first longitudinal center line. A cutting head projects integrally from at least one end of the shaft. The cutting head includes a cutting edge forming an angle of 45° with the first longitudinal center line. Alternatively, or additionally, the cutting head defines a second longitudinal center line forming an angle of 45° with the first longitudinal center line.