Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template nucleic acid, a template switch oligonucleotide, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template nucleic acid and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid including a region polymerized from the dNTPs by the polymerase. The methods further include attaching sequencing platform adapter constructs to ends of the product nucleic acid or a derivative thereof. Aspects of the invention further include compositions and kits.

Abstract: Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template ribonucleic acid (RNA), a template switch oligonucleotide including a 3? hybridization domain and a sequencing platform adapter construct, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template RNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.

Abstract: Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (DNA), a polymerase, a template switch oligonucleotide, and dNTPs into a reaction mixture. The components are combined into the reaction mixture under conditions sufficient to produce a product nucleic acid that includes the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.

Abstract: Provided are methods of depleting a target molecule in a sample. The methods include contacting a target molecule with a free radical-generating system and generating free radicals from the free radical-generating system to deplete the target molecule in the sample. Kits for practicing the subject methods are also provided.

Abstract: A method of assigning a priority to a document in a content-routed network including a plurality of routers interconnected by links, involves matching rules against the content of a received document to assign a priority, mapping the priority onto an egress link, and mapping the egress links onto the interconnect network between content routers to achieve end-to-end quality of service.

Abstract: A method of managing a content routed network, involves distributing published documents through said network for delivery to subscribers; maintaining data logs pertaining to said published documents at different points in the network; and correlating the data logs to obtain information about the operation of the network.

Abstract: In a method of managing a content-based network including a plurality of content routers, such as XML routers, dynamic links are established between the content routers which are not direct neighbors.

Abstract: A carrier grade content router, includes a distinct management plane for housing externally visible management applications, and coordinating and relaying external management requests to appropriate underlying application code in the router; a distinct control plane for running control protocols required within an XML routed network; and a distinct data plane for receiving and forwarding customer data. Some functions can be implemented in software or via a hardware accelerator.

Abstract: In a method of managing a content-based network, which is typically XML-based, and optionally may be overlaid on an underlying network having a plurality of network elements interconnected by links, a link state protocol maintains each network element's topological view of the overlay network from the underlying network. A subscription management protocol ensures dissemination of published content within the content-based network independently of the link state protocol.

Abstract: A method of adding meta-data to document in a content-routed network including a plurality of routers interconnected by links involves adding an encoded meta-tag to the document.

Abstract: An implicit routing protocol for content-based networks having a plurality of XML routers includes an XML Link State Protocol and an XML Subscription Management Protocol that routes customer data based on XML content. The XML Link State Protocol and the XML Subscription Management Protocol includes several messages that must be exchanged between XML routers in the network. These messages are encoded using XML.

Abstract: A method for exchanging control and customer data between network element in a communications network involves establishing a virtual connection between the routers, and exchanging the control and customer data over an http layer.

Abstract: A method of managing a content routed network, involves distributing published documents through said network for delivery to subscribers; maintaining data logs pertaining to said published documents at different points in the network; and correlating the data logs to obtain information about the operation of the network.

Abstract: A method of assigning a priority to a document in a content-routed network including a plurality of routers interconnected by links, involves matching rules against the content of a received document to assign a priority, mapping the priority onto an egress link, and mapping the egress links onto the interconnect network between content routers to achieve end-to-end quality of service.

Abstract: An implicit routing protocol for content-based networks having a plurality of XML routers includes an XML Link State Protocol and an XML Subscription Management Protocol that routes customer data based on XML content. The XML Link State Protocol and the XML Subscription Management Protocol includes several messages that must be exchanged between XML routers in the network. These messages are encoded using XML.

Abstract: In a method of managing a content-based network, which is typically XML-based, and optionally may be overlaid on an underlying network having a plurality of network elements interconnected by links, a link state protocol maintains each network element's topological view of the overlay network from the underlying network. A subscription management protocol ensures dissemination of published content within the content-based network independently of the link state protocol.

Abstract: In a method of managing a content-based network including a plurality of content routers, such as XML routers, dynamic links are established between the content routers which are not direct neighbors.

Abstract: A carrier grade content router, includes a distinct management plane for housing externally visible management applications, and coordinating and relaying external management requests to appropriate underlying application code in the router; a distinct control plane for running control protocols required within an XML routed network; and a distinct data plane for receiving and forwarding customer data. Some functions can be implemented in software or via a hardware accelerator.

Abstract: A method for exchanging control and customer data between network element in a communications network involves establishing a virtual connection between the routers, and exchanging the control and customer data over an http layer.

Abstract: A method of adding meta-data to document in a content-routed network including a plurality of routers interconnected by links involves adding an encoded meta-tag to the document.