Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH<7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.

Abstract: A system and method for additive manufacturing are described. A system includes an additive manufacturing tooling head configured to deposit a heated material to form a workpiece, a tooling controller configured direct the tooling head through a sequence of additive manufacturing process actions, and a chamber comprising a build surface to support the workpiece during the sequence of additive manufacturing process actions. At least one thermal medium is positioned within the chamber. The tooling controller is further configured to determine a thermal gradient between the workpiece and the portion of the heated material based on the temperature of the workpiece and the temperature of the portion of the heated material to be deposited, and control the at least one thermal medium contained within the chamber over time based at least in part on a predetermined thermal gradient range.

Abstract: A negative pressure wound therapy device can include one or more fluid detection systems. A canister fluid level detection system can incorporate various fluid detection devices to communicate data relating to the fluid level of the canister. In some cases, a negative pressure wound therapy device can include a device housing, a negative pressure source, and a canister configured to be in fluid communication with the negative pressure source. The canister can include a canister housing configured to store fluid aspirated from a wound, a cap connected to the canister housing, and a fluid level sensor supported by the cap. The fluid level sensor can be configured to detect a completed electrical circuit when the fluid aspirated from the wound comes into contact with the sensor. An electronic circuitry can be configured to detect a state of the sensor and provide an indication of a status of the canister.

Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

Abstract: The present invention provides engineered protease polypeptides and compositions thereof. The engineered protease polypeptides have been optimized to provide improved activity, improved thermostability, protease stability, autolytic stability, and stability under a range of pH conditions, including acidic (pH<7) and basic (pH>7) conditions. The invention also relates to the use of the compositions comprising the engineered protease polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered protease polypeptides, as well as methods for making the engineered polynucleotides and protease polypeptides.

Abstract: Systems and methods for balancing load in an area of a wireless network comprising first nodes operating according to a first Radio Access Technology and at least one second node operating according to a second Radio Access Technology. The method comprises establishing a connection between a user device and a first node; receiving an indication of an entry threshold for establishing a connection between the user device and a second node during a current time period, wherein the entry threshold is determined in dependence on an expected usage requirement for the area of the wireless network during the current time period; determining, by the user device, whether a parameter of a signal received from the second node exceeds the entry threshold; and responsive to determining that the parameter of the signal received from the second node exceeds the entry threshold, establishing, by the first node, a dual connectivity session between the user device and both the first and the second nodes.

Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH <7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.

Abstract: The present invention relates to a non-human animal model of chronic glaucoma. In addition, the invention refers to a method for the preparation of said animal model, as well as to the use thereof.

Abstract: In general, one aspect disclosed features a computer-implemented method comprising: determining whether Global Positioning System (GPS) location data are available; responsive to determining the GPS location data are available, causing transmission of a first message in National Marine Electronics Association (NMEA) format, the first message comprising the GPS location data; and responsive to determining the GPS location data are not available: determining a location based on non-GPS location data, generating a second message in NMEA format, the second message representing the determined location, and transmitting the second message.

Abstract: A composition containing ammonium polyphosphate and at least one alkali metal salt or alkaline-earth metal salt are provided along with a process for preparing the composition and uses of the composition as a flame retardant and/or coating material.

Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH<7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.

Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

Abstract: A method and system for deploying an application from a deployment source to a device may include determining if the application is available for deployment to the device from a plurality of deployment sources, upon determining that the application is available for deployment from the plurality of deployment sources, identifying one of the plurality of deployment sources as a preferred deployment source for deploying the application; and deploying the application from the preferred deployment source to the device. Deploying the application may include installing the application, loading one or more user interface elements associated with the application and activating the application.

Abstract: A method for manufacturing electrically conductive structures, preferably conductive pathway structures using laser beams on a non-conductive carrier (LDS method), wherein a non-conductive carrier material is provided which contains at least one inorganic metal phosphate compound and at least one stabiliser finely distributed or dissolved therein, the carrier material is irradiated in regions by laser beams generating the electrically conductive structures in the irradiated regions.

Abstract: The invention relates to a process for producing copper(II) hydroxide phosphate from a copper(II) compound and phosphoric acid, to a copper(II) hydroxide phosphate obtainable by this process and to the use of an additive in the production of copper(II) hydroxide phosphate.

Abstract: A joystick assembly for a working machine having a working arm includes a controller configured to control a plurality of machine functions, a first electronic joystick in communication with the controller, and actuators in communication with the controller. Each actuator is configured to actuate a function associated with the working arm. The first electronic joystick comprises four axes of movement, and the first electronic joystick is configured to transmit electronic signals to the controller in response to being displaced along an axis from a neutral position. The controller is configured to receive the electronic signals from the first electronic joystick, and to transmit an electrical signal to one or more actuators to actuate the actuators. The joystick assembly is configured such that the controller actuates a different actuator for controlling a different function associated with the working arm, dependent upon the axis of displacement of the first electronic joystick.

Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

Abstract: An apparatus includes a machine for manufacturing a product from one or more input materials, with the machine including at least a sensor, a control panel, and a switch through which operation of the machine can be stopped. The apparatus further includes a physical display device and a computing device communicationally coupled to both the machine and the physical display device, the computing device causing the physical display device to display user interface that has a first portion displaying an availability indicator, a second portion displaying a performance indicator and a third portion displaying a quality indicator. The computing device can determine the availability indicator, the performance indicator and the quality indicator from data received from the machine and from the operator thereof. Restarting of the machine can be prevented until a reason for the machine's stoppage is received, with the reason being optionally computer-validated to enable restart.