Abstract: The present disclosure relates to methods for reducing the oxidation level of cysteine residues in recombinant polypeptides such as anti-IL-17 antibodies during cell culture (e.g., a preparation of secukinumab antibodies) that have been recombinantly produced by mammalian cells. Also provided are purified preparations of recombinant polypeptides such as anti-IL-17 antibodies or antigen binding fragments thereof produced by such methods, e.g, purified preparations of secukinumab. Also provided are purified preparations of recombinant polypeptides produced by such methods wherein the level of active recombinant polypeptide in the preparation is high.

Abstract: A method and system for deploying an application from a deployment source to a device may include determining if the application is available for deployment to the device from a plurality of deployment sources, upon determining that the application is available for deployment from the plurality of deployment sources, identifying one of the plurality of deployment sources as a preferred deployment source for deploying the application; and deploying the application from the preferred deployment source to the device. Deploying the application may include installing the application, loading one or more user interface elements associated with the application and activating the application.

Abstract: The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

Abstract: The present invention provides engineered ?-glucosidase (BGL) enzymes, polypeptides having BGL activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the enzymes, and methods of using the engineered BGL enzymes to make products with ?-glucose linkages.

Abstract: The present invention provides engineered transaminase polypeptides useful for the synthesis of chiral amine compounds under industrially relevant conditions. The invention also provides polynucleotides encoding the engineered transaminase polypeptides, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases for the production of chiral amine compounds.

Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

Abstract: The present application provides engineered glucose dehydrogenase polypeptides having imine reductase activity, polynucleotides encoding the engineered polypeptides, host cells capable of expressing the engineered polypeptides, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.

Abstract: The present invention provides engineered ?-glucosidase (BGL) enzymes, polypeptides having BGL activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. The present invention also provides compositions comprising the enzymes, and methods of using the engineered BGL enzymes to make products with ?-glucose linkages.

Abstract: The present invention provides engineered protease polypeptides and compositions thereof. The engineered protease polypeptides have been optimized to provide improved activity, improved thermostability, protease stability, autolytic stability, and stability under a range of pH conditions, including acidic (pH<7) and basic (pH>7) conditions. The invention also relates to the use of the compositions comprising the engineered protease polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered protease polypeptides, as well as methods for making the engineered polynucleotides and protease polypeptides.

Abstract: The present invention provides engineered amylase polypeptides and compositions thereof. The engineered amylase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH <7) conditions. The invention also relates to the use of the compositions comprising the engineered amylase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered amylase polypeptides, as well as methods for making the engineered polynucleotides and amylase polypeptides.

Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.

Abstract: Many computing systems process data organized in a matrix format. For example, artificial neural networks (ANNs) perform numerous computations on data organized into matrices using conventional matrix arithmetic operations. One such operation, which is commonly performed, is the transpose operation. Additionally, many such systems need to process many matrices and/or matrices that are large in size. For sparse matrices that hold few significant values and many values that can be ignored, transmitting and processing all the values in such matrices is wasteful. Thus, techniques are introduced for storing a sparse matrix in a compressed format that allows for a matrix transpose operation to be performed on the compressed matrix without having to first decompress the compressed matrix. By utilizing the introduced techniques, more matrix operations can be performed than conventional systems.

Abstract: The present invention provides engineered penicillin G acylase (PGA) enzymes having improved properties, polynucleotides encoding such enzymes, compositions including the enzymes, and methods of using the enzymes.

Abstract: In general, one aspect disclosed features a computer-implemented method comprising: determining whether Global Positioning System (GPS) location data are available; responsive to determining the GPS location data are available, causing transmission of a first message in National Marine Electronics Association (NMEA) format, the first message comprising the GPS location data; and responsive to determining the GPS location data are not available: determining a location based on non-GPS location data, generating a second message in NMEA format, the second message representing the determined location, and transmitting the second message.

Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. Methods for producing PAL enzymes are also provided. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activities that are useful under industrial process conditions for the production of pharmaceutical compounds.

Abstract: Systems and methods for balancing load in an area of a wireless network comprising first nodes operating according to a first Radio Access Technology and at least one second node operating according to a second Radio Access Technology. The method comprises establishing a connection between a user device and a first node; receiving an indication of an entry threshold for establishing a connection between the user device and a second node during a current time period, wherein the entry threshold is determined in dependence on an expected usage requirement for the area of the wireless network during the current time period; determining, by the user device, whether a parameter of a signal received from the second node exceeds the entry threshold; and responsive to determining that the parameter of the signal received from the second node exceeds the entry threshold, establishing, by the first node, a dual connectivity session between the user device and both the first and the second nodes.

Abstract: The present disclosure is directed to methods for proteome engineering cells such that cell-free extracts prepared from such engineered cells can be modified to have metabolic flux directed to a metabolism of interest. In addition, methods for producing cell-free extracts with directed metabolism, cell-free extracts and kits that contain cell-free extracts are also disclosed.

Abstract: A training machine to generate training data for supervised machine learning. The training machine is a working machine with a camera, a sensor, a body, and a moveable element moveable relative to the body. Training data is generated by capturing images, using the camera of at least a portion of the moveable element; and determining, using the sensor, sensor data which indicates the position of the moveable element in the image. The training data is used to train a machine learning algorithm to determine a position of a moveable element based on an image of at least a portion of the moveable element, without the need for corresponding sensor data.

Abstract: A medical device for the insertion of a tube, with which a tube is introduced from the oral or nasal cavity to the oesophagus of a patient. The device includes tubing and an inlet body, the inlet body is adapted to be arranged between the posterior pharynx and the upper third of the oesophagus of a patient, and the inlet body is joined to the tubing at a point along the vertical dimension thereof; the tubing and the inlet body also being in connection through a through hole which communicates them internally.

Abstract: Many computing systems process data organized in a matrix format. For example, artificial neural networks (ANNs) perform numerous computations on data organized into matrices using conventional matrix arithmetic operations. One such operation, which is commonly performed, is the transpose operation. Additionally, many such systems need to process many matrices and/or matrices that are large in size. For sparse matrices that hold few significant values and many values that can be ignored, transmitting and processing all the values in such matrices is wasteful. Thus, techniques are introduced for storing a sparse matrix in a compressed format that allows for a matrix transpose operation to be performed on the compressed matrix without having to first decompress the compressed matrix. By utilizing the introduced techniques, more matrix operations can be performed than conventional systems.