Abstract: Various embodiments disclosed relate to a composite shingle. The composite shingle includes a particle layer and a polyketone layer proximate to the particle layer.

Abstract: Various aspects of the present disclosure relate to a sealed storage tank. The sealed storage tank includes a first film. The first film includes a first polymeric layer having a thickness in a range of from about 0.05 mm to about 1 mm. The first film further includes a first fibrous scrim layer directly contacting the first polymeric layer. The first fibrous scrim layer has a denier value in a range of from about 500 denier to about 1500 denier. The sealed storage tank further includes a second film. The second film includes a second polymeric layer having a thickness in a range of from about 0.05 mm to about 1 mm. The second film further includes a second fibrous scrim layer directly contacting the second polymeric layer. The second fibrous scrim layer has a denier value in a range of from about 500 denier about 1500 denier.

Abstract: Various aspects of the present disclosure relate to a sealed storage tank. The sealed storage tank includes a polymeric layer having a thickness in a range of from about 0.05 mm to about 1 mm. The sealed storage tank further includes a fibrous scrim layer directly contacting the polymeric layer. The fibrous scrim layer includes a denier value in a range of from about 500 denier to about 1500 denier. The polymeric layer is optionally substantially translucent or transparent and is optionally at least partially visible through the fibrous scrim layer.

Abstract: Aspects of the present disclosure relate to methods and compositions related to the preparation of immune cells, including engineered T cells comprising at least one exogenous ?? T cell receptor, for example one that is selected to target a specific disease or pathogen (e.g., cancer or COVID-19). The T cells may be produced from human hematopoietic stem/progenitor cells and are suitable for allogeneic cellular therapy because they do not induce graft-versus-host disease (GvHD) and resist host immune allorejection. Consequently, such cells are suitable for off-the-shelf use in clinical therapy.

Abstract: A technique includes a first compute node of a cluster of nodes receiving a communication from a cluster maintenance node of the cluster instructing the first compute node to provide an installation image that is received by the first compute node to a second compute node of the cluster. The first node and the second node are peers. The technique includes sharing the first installation stream that communicates the image to the first compute node. Sharing the first installation stream includes, while the first compute node is receiving the first installation stream, the first compute node providing a second installation stream to communicate parts of the image received via the first installation stream to the second compute node.

Abstract: The present invention is toward a base oil or lubricant additives, methods of using the same, lubricant compositions including the same, and methods of forming the lubricant compositions. A base oil or lubricant additive has Structure I or Structure II as described herein.

Abstract: Various embodiments disclosed relate to a composite shingle. The composite shingle includes a particle layer and a polyketone layer proximate to the particle layer.

Abstract: Methods for making a synthetic nucleic acid which comprise: (a) identifying a conflicting nucleotide sequence in a target sequence; (b) inserting a masking sequence into the conflicting sequence to produce a disrupted target sequence, wherein: (i) the masking sequence comprises recognition sites for one or more Type IIS restriction endonucleases; and (ii) digestion of said disrupted target sequence by said one or more Type IIS restriction endonucleases followed by re-ligation reconstitutes the target sequence; (c) synthesizing a polynucleotide comprising the disrupted target sequence using polymerase chain assembly; and (d) removing the masking sequence from said polynucleotide by digesting said polynucleotide with said one or more Type IIS restriction endonucleases followed by re-ligation of the digestion product, thereby producing a polynucleotide comprising said target sequence.

Abstract: Various embodiments disclosed relate to a composite shingle. The composite shingle includes a particle layer and a polyketone layer proximate to the particle layer.

Abstract: A system for forming a tendon sleeve on an atmospheric balloon is described herein, the system comprising a coupling assembly including a seam coupling mechanism that forms a seam joint between a first gore panel and a second gore panel, the seam joint is spaced from respective first and second lateral edges of the first and second gore panels to form first and second edge flanges, and an edge coupling mechanism that forms an edge joint between the first and second edge flanges and closes a tendon sleeve, the edge joint spaced from the seam joint, the tendon sleeve includes a tendon sleeve passage between the seam joint and the edge joint and between the first and second edge flanges. The system also includes a tendon positioning mechanism that positions a tendon within the tendon sleeve passage. A tendon sleeve formed by this system is also described herein.

Abstract: A roller coaster with a vehicle rolling under gravity along a track defining a ride path. The vehicle includes a chassis coupled to the track to roll on one or more surfaces of the track, and the vehicle includes a passenger compartment mounted on the chassis. The coaster also includes a compartment positioning mechanism that operates to move, such as with yaw, the passenger compartment between a first position in a passenger load/unload section of the ride path and one or more differing positions in the gravity-based ride section of the ride path. The coaster includes a power supply assembly with a charging element mounted on the track. The power supply assembly includes an onboard energy storage mounted on the vehicle that is charged by the charging element. The onboard energy storage powers the compartment positioning mechanism to move the passenger compartment between the first and second positions.

Abstract: This disclosure provides, among other things, a method of combining nucleic acid fragments, comprising: (a) providing two double-stranded DNA molecules with a common sequence, wherein the common sequence is at the end of each molecule; (b) nicking one strand in the common sequence of both molecules at a respective nicked site; (c) moderately denaturing both molecules to remove a single-stranded fragment from the nicked site to one end of each molecule, wherein the single-stranded fragment includes the common sequence in part or in whole, resulting in an overhanging sequence in each molecule, and the overhanging sequences in both molecules are complementary to each other; (d) allowing the overhanging sequences of both molecules to anneal to each other, and ligating the molecules. Alternative ways for performing the method are also provided.

Abstract: A track-based swing ride system providing passengers a unique swinging ride experience. The ride system includes a ride track that supports a moving bogie coupled to the ride track that includes a drive assembly driving the bogie along a ride path defined by the ride track. The ride apparatus includes a pendulum arm pivotally coupled at a first end to the bogie, whereby the pendulum arm is suspended below the ride track and moves along the ride path with the bogie. The ride apparatus includes a passenger vehicle coupled to a second end of the pendulum arm opposite the first end of the pendulum arm. To provide the swinging sensation, the ride apparatus includes a roll driver rotating, concurrently with the drive assembly of the bogie, the pendulum arm about a roll axis extending through the first end of the pendulum arm.

Abstract: A roller coaster with a vehicle rolling under gravity along a track defining a ride path. The vehicle includes a chassis coupled to the track to roll on one or more surfaces of the track, and the vehicle includes a passenger compartment mounted on the chassis. The coaster also includes a compartment positioning mechanism that operates to move, such as with yaw, the passenger compartment between a first position in a passenger load/unload section of the ride path and one or more differing positions in the gravity-based ride section of the ride path. The coaster includes a power supply assembly with a charging element mounted on the track. The power supply assembly includes an onboard energy storage mounted on the vehicle that is charged by the charging element. The onboard energy storage powers the compartment positioning mechanism to move the passenger compartment between the first and second positions.

Abstract: Methods for making a synthetic nucleic acid which comprise: (a) identifying a conflicting nucleotide sequence in a target sequence; (b) inserting a masking sequence into the conflicting sequence to produce a disrupted target sequence, wherein: (i) the masking sequence comprises recognition sites for one or more Type IIS restriction endonucleases; and (ii) digestion of said disrupted target sequence by said one or more Type IIS restriction endonucleases followed by re-ligation reconstitutes the target sequence; (c) synthesizing a polynucleotide comprising the disrupted target sequence using polymerase chain assembly; and (d) removing the masking sequence from said polynucleotide by digesting said polynucleotide with said one or more Type IIS restriction endonucleases followed by re-ligation of the digestion product, thereby producing a polynucleotide comprising said target sequence.

Abstract: Provided herein is a method comprising: producing a first complex by annealing: a first nucleic acid comprising, in order, a first unique sequence, a first central sequence and a second unique sequence; and a second nucleic acid comprising, in order, said second unique sequence, a second central sequence and a third unique sequence; wherein the first, second and third unique sequences do not hybridize with each other; subjecting said first complex to multiple rounds of primer extension to extend the first and second nucleic acids using each other as a template, thereby producing a first product molecule that contains, in order, the first unique sequence, the first central sequence, the second unique sequence, the second central sequence and the third unique sequence; and circularizing said first product molecule by intramolecularly ligating the ends of said product molecule together. Kits and compositions relating to the method are also provided.

Abstract: A method for fragmenting a genome is provided. In certain embodiments, the method comprises: (a) combining a genomic sample containing genomic DNA with a plurality of Cas9-gRNA complexes, wherein the Cas9-gRNA complexes comprise a Cas9 protein and a set of at least 10 Cas9-associated guide RNAs that are complementary to different, pre-defined, sites in a genome, to produce a reaction mixture; and (b) incubating the reaction mixture to produce at least 5 fragments of the genomic DNA. Also provided is a composition comprising at least 100 Cas9-associated guide RNAs that are each complementary to a different, pre-defined, site in a genome. Kits for performing the method are also provided. In addition, other methods, compositions and kits for manipulating nucleic acids are also provided.

Abstract: A heat pump water heater and systems and methods for its control. The systems are configured to heat water within a water storage tank of a heat pump water heater wherein a controller within the system is operatively connected to a plurality of heat sources including at least one electric heating element and a heat pump and sensors in order to selectively energize one of the plurality of heat sources. The controller is configure to process data representative of the temperature of water within the tank near the top of the water storage tank, and rate of water flowing out of the water storage tank, in order to automatically selectively energize the heat sources. The selection of heat sources by the controller is determined by a mode of operation selected by the user and the data processed by the controller in view of the selected mode of operation.

Abstract: A track-based swing ride system providing passengers a unique swinging ride experience. The ride system includes a ride track that supports a moving bogie coupled to the ride track that includes a drive assembly driving the bogie along a ride path defined by the ride track. The ride apparatus includes a pendulum arm pivotally coupled at a first end to the bogie, whereby the pendulum arm is suspended below the ride track and moves along the ride path with the bogie. The ride apparatus includes a passenger vehicle coupled to a second end of the pendulum arm opposite the first end of the pendulum arm. To provide the swinging sensation, the ride apparatus includes a roll driver rotating, concurrently with the drive assembly of the bogie, the pendulum arm about a roll axis extending through the first end of the pendulum arm.