Patents by Inventor Donald E. Ingber

Donald E. Ingber has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11939608
    Abstract: Described herein are heme-binding compositions and methods relating to their use, for example methods of treatment of sepsis and rhabdomyolysis.
    Type: Grant
    Filed: March 18, 2022
    Date of Patent: March 26, 2024
    Inventors: Michael Super, Alexander L. Watters, Philip T. Snell, Donald E. Ingber
  • Patent number: 11940441
    Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.
    Type: Grant
    Filed: September 11, 2020
    Date of Patent: March 26, 2024
    Assignee: President and Fellows of Harvard College
    Inventors: Donald E. Ingber, Kambez Hajipouran Benam, Remi Villenave, Geraldine A. Hamilton, Bryan Hassell, Christopher D. Hinojosa, Carolina Lucchesi
  • Publication number: 20240093190
    Abstract: The present disclosure provides compositions and methods for inhibiting viral pathogenesis by targeting long noncoding ribonucleic acids.
    Type: Application
    Filed: January 18, 2022
    Publication date: March 21, 2024
    Applicant: President and Fellows of Harvard College
    Inventors: Longlong Si, Haiqing Bai, Rachelle Pranti-Baun, Donald E. Ingber
  • Publication number: 20240084235
    Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.
    Type: Application
    Filed: September 19, 2023
    Publication date: March 14, 2024
    Inventors: Jose Fernandez-Alcon, Norman Wen, Richard Novak, Donald E. Ingber, Geraldine A. Hamilton, Christopher Hinojosa, Karel Domansky, Daniel Levner, Guy Thompson, II, Kambez Hajipouran Benam, Remi Villenave, Thomas Umundum, Alfred Paris, Georg Bauer
  • Publication number: 20240075012
    Abstract: The present disclosure provides compositions and methods for inhibiting respiratory viral infections, inflammatory diseases, and/or respiratory inflammation.
    Type: Application
    Filed: August 11, 2023
    Publication date: March 7, 2024
    Applicant: President and Fellows of Harvard College
    Inventors: Haiqing Bai, Longlong Si, Rachelle Prantil-Baun, Donald E. Ingber
  • Publication number: 20240076595
    Abstract: Systems and methods for producing and using a body having a first structure defining a first chamber, a second structure defining a second chamber, a membrane located at an interface region between the first chamber and the second chamber to separate the first chamber from the second chamber. The first chamber comprises a first permeable matrix disposed therein and the first permeable matrix comprises at least one or a plurality of lumens each extending therethrough, which is optionally lined with at least one layer of cells. The second chamber can comprise cells cultured therein. The systems and methods described herein can be used for various applications, including, e.g., growth and/or differentiation of primary cells, and/or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents). The systems and methods can also permit co-cultures of two or more different cell types.
    Type: Application
    Filed: November 9, 2023
    Publication date: March 7, 2024
    Inventors: Donald E. INGBER, Andries VAN DER MEER, Anna HERLAND
  • Patent number: 11919971
    Abstract: This disclosure provides, e.g., methods for coupling Formation of Surface Carboxylates on PES an entity to a solid substrate. The method can comprise treating the with Exposure Time substrate with a plasma, e.g., a CO2 plasma, to increase its reactivity. The entity can be, e.g., a biological polymer that binds a microbe. Substrates produced by these methods can be used in a variety of applications, including hemodialysis and diagnostic assays.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: March 5, 2024
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Daniel Christopher Leslie, Thomas Doyle, Anna Waterhouse, Melissa Rodas, Alexander L. Watters, Michael Super, Donald E. Ingber
  • Patent number: 11884938
    Abstract: The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.
    Type: Grant
    Filed: November 19, 2019
    Date of Patent: January 30, 2024
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. Ingber, Hyun Jung Kim
  • Patent number: 11865536
    Abstract: An organ-on-a-chip microfluidic device is disclosed that mimics a human lymph node and/or human lymphoid tissue. The device can include cells from human blood and lymphatic tissue, include an extracellular matrix for the development of immune system components, and provide for the perfusion of fluids and solids resembling blood and lymphatic fluid within micrometer sized channels.
    Type: Grant
    Filed: April 27, 2022
    Date of Patent: January 9, 2024
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Girija Goyal, Donald E. Ingber
  • Publication number: 20240002808
    Abstract: A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.
    Type: Application
    Filed: July 25, 2023
    Publication date: January 4, 2024
    Inventors: Richard Novak, Sasan Jalili-Firoozinezhad, Francesca S. Gazzaniga, Elizabeth Calamari, Diogo M. Camacho, Bret Andrew Nestor, Cicely Fadel, Michael J. Cronce, Dennis Kasper, Donald E. Ingber, Amir Bein
  • Publication number: 20240002679
    Abstract: Method for making a coating on a surface of a substrate are described herein. The methods include applying a mixture to a surface of a substrate while maintaining the substrate at an elevated temperature. The mixture includes a particulate material and a proteinaceous material.
    Type: Application
    Filed: November 29, 2021
    Publication date: January 4, 2024
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Pawan JOLLY, Sanjay Sharma TIMILSINA, Nolan DURR, Donald E. INGBER
  • Publication number: 20230416661
    Abstract: System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.
    Type: Application
    Filed: September 7, 2023
    Publication date: December 28, 2023
    Inventors: Donald E. INGBER, Dongeun HUH
  • Publication number: 20230416691
    Abstract: Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.
    Type: Application
    Filed: September 6, 2023
    Publication date: December 28, 2023
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. INGBER, Magdalena KASENDRA, Alexandra SONTHEIMER-PHELPS, Alessio TOVAGLIERI
  • Publication number: 20230416662
    Abstract: System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.
    Type: Application
    Filed: September 7, 2023
    Publication date: December 28, 2023
    Inventors: Donald E. INGBER, Dongeun HUH
  • Patent number: 11845920
    Abstract: The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.
    Type: Grant
    Filed: March 26, 2018
    Date of Patent: December 19, 2023
    Assignees: President and Fellows of Harvard College, The General Hospital Corporation
    Inventors: David Benson Chou, Liliana S. Teixeira Moreira Leijten, Arianna Rech, Richard Novak, Donald E. Ingber, Yuka Milton, Viktoras Frismantas, Oren Levy
  • Publication number: 20230358740
    Abstract: The technology described herein is directed to methods for detection of microbes and microbe components. In some embodiments of any of the aspects, the methods comprise methods of microbe isolation, sample preparation, mass spectrometry, or analysis. In some embodiments of any of the aspects, such methods can be applied to detect at least one microbe or at least one microbial component in a sample, including not limited to a patient sample, an animal model sample, an environmental sample, or a non-biological sample.
    Type: Application
    Filed: January 8, 2021
    Publication date: November 9, 2023
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Mark Joseph CARTWRIGHT, Michael SUPER, Donald E. INGBER, Jennifer GRANT, Justin SCOTT, Shannon Catherine DUFFY, Sahil LOOMBA
  • Publication number: 20230357691
    Abstract: System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.
    Type: Application
    Filed: June 30, 2023
    Publication date: November 9, 2023
    Inventors: Donald E. INGBER, Dongeun HUH
  • Publication number: 20230356216
    Abstract: An organ-on-a-chip microfluidic device is disclosed that mimics a human lymph node and/or human lymphoid tissue. The device can include cells from human blood and lymphatic tissue, include an extracellular matrix for the development of immune system components, and provide for the perfusion of fluids and solids resembling blood and lymphatic fluid within micrometer sized channels.
    Type: Application
    Filed: June 30, 2023
    Publication date: November 9, 2023
    Inventors: Girija Goyal, Donald E. Ingber
  • Publication number: 20230357690
    Abstract: The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.
    Type: Application
    Filed: June 28, 2023
    Publication date: November 9, 2023
    Applicants: President and Fellows of Harvard College, The General Hospital Corporation
    Inventors: David Benson Chou, Liliana S. Teixeira Moreira Leijten, Arianna Rech, Richard Novak, Donald E. Ingber, Yuka Milton, Viktoras Frismantas, Oren Levy
  • Patent number: 11807677
    Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can not only bind or capture of a microbe or microbial matter thereof, but they also have improved capability (e g, enhanced sensitivity or signal intensity) of detecting a microbe or microbial matter. Thus, the microbe-targeting molecules, microbe-targeting articles, and/or the kit described herein can be used in various applications, e.g., but not limited to assays for detection of a microbe or microbial matter, diagnostic and/or therapeutic agents for diagnosis and/or treatment of an infection caused by microbes in a subject or any environmental surface, and/or devices for removal of a microbe or microbial matter from a fluid.
    Type: Grant
    Filed: December 16, 2021
    Date of Patent: November 7, 2023
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Alexander Watters, Brendon Dusel, Michael Super, Mark Cartwright, Donald E. Ingber