Patents by Inventor Donald E. Ingber

Donald E. Ingber has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240350548
    Abstract: Embodiments of various aspects described herein relate to methods, kits, and cell culture media for generation of podocytes from pluripotent stem (PS) cells, as well as cells produced by the same, and methods of use.
    Type: Application
    Filed: April 5, 2024
    Publication date: October 24, 2024
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. INGBER, Samira MUSAH
  • Patent number: 12104174
    Abstract: Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.
    Type: Grant
    Filed: September 13, 2017
    Date of Patent: October 1, 2024
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. Ingber, Magdalena Kasendra, Alexandra Sontheimer-Phelps, Alessio Tovaglieri
  • Publication number: 20240309074
    Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface.
    Type: Application
    Filed: September 14, 2023
    Publication date: September 19, 2024
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. INGBER, Michael SUPER, Jeffrey Charles WAY, Mark J. CARTWRIGHT, Julia B. BERTHET, Dinah R. SUPER, Martin ROTTMAN, Alexander L WATTERS
  • Patent number: 12065634
    Abstract: Systems and methods interconnect cell culture devices and/or fluidic devices by transferring discrete volumes of fluid between devices. A liquid-handling system collects a volume of fluid from at least one source device and deposits the fluid into at least one destination device. In some embodiments, a liquid-handling robot actuates the movement and operation of a fluid collection device in an automated manner to transfer the fluid between the at least one source device and the at least one destination device. In some cases, the at least one source device and the at least one destination device are cell culture devices. The at least one source device and the at least one destination device may be microfluidic or non-microfluidic devices. In some cases, the cell culture devices may be microfluidic cell culture devices. In further cases, the microfluidic cell culture devices may include organ-chips.
    Type: Grant
    Filed: July 22, 2022
    Date of Patent: August 20, 2024
    Assignee: President and Fellows of Harvard College
    Inventors: Donald E. Ingber, Daniel Levner, Guy Thompson, II, Jose Fernandez-Alcon, Christopher David Hinojosa
  • Publication number: 20240248077
    Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.
    Type: Application
    Filed: February 8, 2024
    Publication date: July 25, 2024
    Inventors: Donald E. Ingber, Kambez Hajipouran Benam, Remi Villenave, Geraldine A. Hamilton, Bryan Hassell, Christopher D. Hinojosa, Carolina Lucchesi
  • Publication number: 20240218309
    Abstract: A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.
    Type: Application
    Filed: March 13, 2024
    Publication date: July 4, 2024
    Inventors: Antonio Varone, Norman Wen, Daniel Levner, Richard Novak, Lori McPartlin, Donald E. Ingber, Youngjae Choe, Lian Leng, Justin K. Nguyen
  • Publication number: 20240206516
    Abstract: The present disclosure generally relates to sugar reduction in foods and, in some aspects, to enzyme-polymer conjugated particles for food and other applications. Certain aspects of the disclosure are directed to compositions for reducing sugar content and/or producing dietary fiber within food products during or after consumption (e.g., in a subject's gastrointestinal (GI) tract), while maintaining the sweetness and flavor of the sugar in food products upon consumption (e.g., in a subject's mouth). For example, in one set of embodiments, a composition may comprise a particle comprising an enzyme capable of converting a sugar into a relatively non-digestible form (e.g., a polymer), optionally an inhibitor that reversibly inhibits the enzyme from converting the sugar, and optionally an additive capable of associating with the inhibitor. The composition may be used for in situ conversion of sugars upon exposure to an environment condition (e.g., pH and/or temperature) in the GI tract.
    Type: Application
    Filed: June 8, 2022
    Publication date: June 27, 2024
    Applicants: President and Fellows of Harvard College, Massachusetts Institute of Technology
    Inventors: Donald E. Ingber, Vaskar Gnyawali, Daneille Harrier, Yan Liu, Evan Minghao Zhao, David A. Weitz, James J. Collins, Adama Marie Sesay, Bobby Tyrell Haney
  • Publication number: 20240182868
    Abstract: Disclosed herein are organ chips that can be individually used or integrated together to form different microphysiological systems, e.g., for use in cell culturing, drug screening, toxicity assays, personalized therapeutic treatment, scaffolding in tissue repair and/or replacement, and/or pharmacokinetic or pharmacodynamics studies.
    Type: Application
    Filed: February 12, 2024
    Publication date: June 6, 2024
    Inventors: Donald E. Ingber, Kevin Kit Parker, Geraldine A. Hamilton, Anthony Bahinski
  • Patent number: 11980641
    Abstract: Embodiments of various aspects described herein relate to methods, kits, and cell culture media for generation of podocytes from pluripotent stem (PS) cells, as well as cells produced by the same, and methods of use.
    Type: Grant
    Filed: July 2, 2021
    Date of Patent: May 14, 2024
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. Ingber, Samira Musah
  • Patent number: 11976304
    Abstract: Described herein are methods for providing an in vitro intestinal model system, e.g., using primary cells instead of cell lines and/or cancerous cells.
    Type: Grant
    Filed: December 1, 2021
    Date of Patent: May 7, 2024
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. Ingber, Magdalena Kasendra, Alexandra Sontheimer-Phelps, Alessio Tovaglieri
  • Publication number: 20240140836
    Abstract: Microfluidic devices for sterilizing fluids using pulsed electric fields are disclosed. In some embodiments, a device may include first and second electrode layers and a spacer layer positioned between the electrode layers. The spacer layer may define one or more fluid channels extending between the electrode layers. A power supply may be coupled to the electrode layers and configured to supply voltage pulses to the electrode layers to generate electric field pulses within the fluid channels. In some embodiments, the electrode layers may be textured such that the electric fields generated in the fluid channels are non-uniform.
    Type: Application
    Filed: September 30, 2020
    Publication date: May 2, 2024
    Applicant: President and Fellows of Harvard College
    Inventors: Elizabeth Calamari, Richard Novak, Manuel Ramses Martinez Flores, Alexandre L.M. Dinis, Robert Cunningham, Olivier Henry, Donald E. Ingber, Jeremy J. Papadopoulos
  • Publication number: 20240108709
    Abstract: The present invention provides vaccine compositions and methods of producing such compositions. Other embodiments of the invention include methods of treating a pathogen infection, methods of vaccinating a subject against a pathogen infection, and methods for treating an antibiotic-resistance bacterial infection in a subject in need thereof. In further embodiments, the invention includes methods of decreasing the level of a pathogen in a subject having a pathogen infection, methods of increasing the surviving rate of a subject having a pathogen infection, methods of reducing the level of pain associated with a pathogen infection, and methods of reducing the level of distress associated with a pathogen infection in a subject in need thereof. Novel scaffold compositions and opsonin-bound or lectin-bound pathogen compositions, and uses thereof, are also provided herein.
    Type: Application
    Filed: March 20, 2023
    Publication date: April 4, 2024
    Applicant: President and Fellows of Harvard College
    Inventors: Michael Super, Edward J. Doherty, Mark Joseph Cartwright, Des White, Alexander Stafford, Omar Abdel-Rahman Ali, Amanda Graveline, Donald E. Ingber, David J. Mooney, Benjamin Seiler
  • Publication number: 20240110159
    Abstract: The embodiments of the invention described herein relate to systems and methods for culturing and/or maintaining intestinal cells, tissues and/or organoids in vitro. The cells, tissues and/or organoids cultured according to the methods and systems described herein can mimic or reproduce natural intestinal epithelial structures and behavior as well as support co-culture of intestinal microflora.
    Type: Application
    Filed: December 11, 2023
    Publication date: April 4, 2024
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. INGBER, Hyun Jung Kim
  • Patent number: 11940441
    Abstract: Provided herein relates to systems and methods for producing and using a body having a central channel separated by one or more membranes. The membrane(s) are configured to divide the central channel into at least one mesochannel and at least one microchannel. The height of the mesochannel is substantially greater than the height of the microchannel. A gaseous fluid can be applied through the mesochannel while a liquid fluid flowing through the microchannel. The systems and methods described herein can be used for various applications, including, e.g., growth and differentiation of primary cells such as human lung cells, as well as any other cells requiring low shear and/also stratified structures, or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents and/or vaccines). The systems and methods can also permit co-culture with one or more different cell types.
    Type: Grant
    Filed: September 11, 2020
    Date of Patent: March 26, 2024
    Assignee: President and Fellows of Harvard College
    Inventors: Donald E. Ingber, Kambez Hajipouran Benam, Remi Villenave, Geraldine A. Hamilton, Bryan Hassell, Christopher D. Hinojosa, Carolina Lucchesi
  • Patent number: 11939608
    Abstract: Described herein are heme-binding compositions and methods relating to their use, for example methods of treatment of sepsis and rhabdomyolysis.
    Type: Grant
    Filed: March 18, 2022
    Date of Patent: March 26, 2024
    Inventors: Michael Super, Alexander L. Watters, Philip T. Snell, Donald E. Ingber
  • Publication number: 20240093190
    Abstract: The present disclosure provides compositions and methods for inhibiting viral pathogenesis by targeting long noncoding ribonucleic acids.
    Type: Application
    Filed: January 18, 2022
    Publication date: March 21, 2024
    Applicant: President and Fellows of Harvard College
    Inventors: Longlong Si, Haiqing Bai, Rachelle Pranti-Baun, Donald E. Ingber
  • Publication number: 20240084235
    Abstract: An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.
    Type: Application
    Filed: September 19, 2023
    Publication date: March 14, 2024
    Inventors: Jose Fernandez-Alcon, Norman Wen, Richard Novak, Donald E. Ingber, Geraldine A. Hamilton, Christopher Hinojosa, Karel Domansky, Daniel Levner, Guy Thompson, II, Kambez Hajipouran Benam, Remi Villenave, Thomas Umundum, Alfred Paris, Georg Bauer
  • Publication number: 20240075012
    Abstract: The present disclosure provides compositions and methods for inhibiting respiratory viral infections, inflammatory diseases, and/or respiratory inflammation.
    Type: Application
    Filed: August 11, 2023
    Publication date: March 7, 2024
    Applicant: President and Fellows of Harvard College
    Inventors: Haiqing Bai, Longlong Si, Rachelle Prantil-Baun, Donald E. Ingber
  • Publication number: 20240076595
    Abstract: Systems and methods for producing and using a body having a first structure defining a first chamber, a second structure defining a second chamber, a membrane located at an interface region between the first chamber and the second chamber to separate the first chamber from the second chamber. The first chamber comprises a first permeable matrix disposed therein and the first permeable matrix comprises at least one or a plurality of lumens each extending therethrough, which is optionally lined with at least one layer of cells. The second chamber can comprise cells cultured therein. The systems and methods described herein can be used for various applications, including, e.g., growth and/or differentiation of primary cells, and/or simulation of a microenvironment in living tissues and/or organs (to model physiology or disease states, and/or to identify therapeutic agents). The systems and methods can also permit co-cultures of two or more different cell types.
    Type: Application
    Filed: November 9, 2023
    Publication date: March 7, 2024
    Inventors: Donald E. INGBER, Andries VAN DER MEER, Anna HERLAND
  • Patent number: 11919971
    Abstract: This disclosure provides, e.g., methods for coupling Formation of Surface Carboxylates on PES an entity to a solid substrate. The method can comprise treating the with Exposure Time substrate with a plasma, e.g., a CO2 plasma, to increase its reactivity. The entity can be, e.g., a biological polymer that binds a microbe. Substrates produced by these methods can be used in a variety of applications, including hemodialysis and diagnostic assays.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: March 5, 2024
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Daniel Christopher Leslie, Thomas Doyle, Anna Waterhouse, Melissa Rodas, Alexander L. Watters, Michael Super, Donald E. Ingber