Abstract: Microfluidic probe head for processing a sequence of separate liquid volumes separated by spacers. The microfluidic probe head includes: an inlet, an outlet, a first fluid channel and a second fluid channel and a fluid bypass connecting the first fluid channel and the second fluid channel. The first fluid channel delivers the sequence of separate liquid volumes from the inlet toward a deposition area, the fluid bypass allows the spacers to be removed from the first fluid channel obtaining a free sequence of separate liquid volumes without spacers, the first fluid channel delivers the free sequence of separate liquid volumes to the deposition area, and the second fluid channel delivers the removed spacers from the fluid bypass to the outlet. The present invention also provides a microfluidic probe and method for processing a sequence of separate liquid volumes.

Abstract: Methods are provided for authenticating a container of items to be sent by a sender to a receiver. The method includes: packing a plurality of items, each having a respective item identifier, in the container; providing a container identifier on the container; and generating a first digital signature by signing a message, comprising the container identifier and each item identifier, using a secret signing key skS of a signing-verification key pair (skS, pkS) of a digital signature scheme. The method further comprises providing the first digital signature for access by the receiver, and sending the container to the receiver. A corresponding verification method comprises accessing the first digital signature, and verifying the first digital signature for the container identifier and the item identifier for each item in the container using the verification key pkS of the sender key pair (skS, pkS).

Abstract: Embodiments of the invention provide a microfluidic chip having microfluidic structures formed on a surface. The structures form an input channel, an output channel, auxiliary channels, and a hydraulic resistor structure connecting the input channel to the output channel via the auxiliary channels. The resistor structure includes N flow resistor portions (N?2), which are connected to the auxiliary channels. The chip further includes at least N?1 actuatable valves, which are arranged in respective ones of the auxiliary channels. The actuation state of the valves can determine the effective hydraulic resistance of the resistor structure. The valves can be electrogates, each including a liquid-pinning trench arranged in a respective one of the auxiliary channels that define a flow path for a liquid introduced therein, so as to form an opening that extends across said flow path. Each electrogate can further include an electrode extending across the flow path.

Abstract: Method, apparatus, and computer program product for a microfluidic channel having a cover opposite its bottom, the cover allowing visual inspection inside the channel, and having electrodes with patterned planar conducting materials, integrated onto its bottom. Using the planar conducting materials, once a fluid sample with suspended microparticles is applied into the channel, highly localized modulated electric field distributions are generated inside the channel and the fluid sample. This generated field causes inducing of dielectrophoretic (DEP) forces such that the DEP forces gradually increase along the length of the channel occupied by the electrodes. These DEP forces counteract the hydrodynamic drag of the flow acting on the particles suspended in the fluid. Because of the induced forces, micro/nano-particles in the fluid sample are deflected at locations in the microchannel that are a function of the particles velocity and this effect is captured by an image sensing device.

Abstract: Microfluidic probe head for processing a sequence of separate liquid volumes separated by spacers. The microfluidic probe head includes: an inlet, an outlet, a first fluid channel and a second fluid channel and a fluid bypass connecting the first fluid channel and the second fluid channel. The first fluid channel delivers the sequence of separate liquid volumes from the inlet toward a deposition area, the fluid bypass allows the spacers to be removed from the first fluid channel obtaining a free sequence of separate liquid volumes without spacers, the first fluid channel delivers the free sequence of separate liquid volumes to the deposition area, and the second fluid channel delivers the removed spacers from the fluid bypass to the outlet. The present invention also provides a microfluidic probe and method for processing a sequence of separate liquid volumes.

Abstract: A method for optically reading information encoded in a microfluidic device, the microfluidic device including an input microchannel, microfluidic modules, and sets of nodes. Nodes of a first set connect the input microchannel to one of the microfluidic modules, and nodes of a second set connect the one of the microfluidic modules to another to form an ordered pair of the microfluidic modules, where the nodes of the first and second sets have different liquid pinning strengths. A liquid loaded into the input microchannel causes an ordered passage of the liquid through each of the microfluidic modules in an order determined by the liquid pinning strengths of the nodes. The passage of the liquid produces an optically readable dynamic pattern which evolves in accordance with the ordered passage of the liquid through the device.

Abstract: The present invention is notably directed to method of fabrication of a microfluidic chip, comprising: providing a substrate, a face of which is covered by an electrically insulating layer; obtaining a resist layer covering one or more selected portions of the electrically insulating layer, at least a remaining portion of said electrically insulating layer not being covered by the resist layer; partially etching with a wet etchant a surface of the remaining portion of the electrically insulating layer to create a recess and/or an undercut under the resist layer; depositing the electrically conductive layer on the etched surface, such that the electrically conductive layer reaches the created recess and/or undercut; and removing the resist layer to expose a portion of the electrically insulating layer adjoining a contiguous portion of the electrically conductive layer. The present invention is further directed to microfluidic chips obtainable by such methods.

Abstract: A diagnostic patch apparatus has a sampling module that includes sampling means for sampling fluid from a patient's skin when the sampling module is placed against the patient's skin, and a sample chamber coupled in fluid communication with the sampling means. The apparatus also has an analysis module that includes a fluid conduit coupled in fluid communication with the sample chamber of the sampling module and a plurality of sensors coupled in fluid communication with the fluid conduit. The apparatus also may have a reader module that includes at least one optical sensor coupled in optical communication with the analysis module, a microcontroller coupled in electrical communication with the at least one sensor of the analysis module, and a wireless communication package coupled in electrical communication with the microcontroller.

Abstract: Microfluidic probe head for processing a sequence of separate liquid volumes separated by spacers. The microfluidic probe head includes: an inlet, an outlet, a first fluid channel and a second fluid channel and a fluid bypass connecting the first fluid channel and the second fluid channel. The first fluid channel delivers the sequence of separate liquid volumes from the inlet toward a deposition area, the fluid bypass allows the spacers to be removed from the first fluid channel obtaining a free sequence of separate liquid volumes without spacers, the first fluid channel delivers the free sequence of separate liquid volumes to the deposition area, and the second fluid channel delivers the removed spacers from the fluid bypass to the outlet. The present invention also provides a microfluidic probe and method for processing a sequence of separate liquid volumes.

Abstract: Methods are provided for producing an authenticated packaged product. The method includes providing on the product a security code encoding security data for the product, packing the product in packaging, and providing a tag carrying encrypted product data on the packaging. The product data comprises the security data for the product. The method further comprises storing a decryption key for the encrypted product data at a network server, and providing on the packaging access data for accessing the decryption key at the network server. Corresponding methods and systems are provided for verifying authenticity of such an authenticated packaged product.

Abstract: A test device is configured for diagnostic testing and includes an optical readable medium, in turn including a pattern of spots of material arranged on a surface of the device. Several patterns may be provided. The patterns accordingly formed may be human and/or machine readable. They may notably encode security information, e.g., indicating whether the device has already been used. The spots may notably be inkjet spotted. In addition, a method is provided for decoding information encoded in a pattern of such a test device. In embodiments, liquid is introduced in the device, which comprises additional spots having a substantially different solubility than spots forming the actual pattern. Thus, the additional spots get solubilized in and flushed by the liquid as the latter wets them, and an initially hidden pattern may be read, which is formed of the remaining spots (not solubilized). Encoding methods are also provided.

Abstract: A microfluidic device includes a surface, which defines a flow path for a liquid, and a liquid inlet, which is in fluid communication with said surface, so as for a liquid introduced via the inlet to advance along a propagation direction on the flow path. Also included are a set of two or more electrical contacts, and a set of electrodes which include sensing portions that extend across the flow path, transversally to the propagation direction. The electrodes are connected to the two or more electrical contacts. Also included are material spots on at least some of the sensing portions of the electrodes. Still, material spots of a same material are only on a subset of the sensing portions of the electrodes, so that the spots can alter an electrical signal detected from the electrical contacts, upon a liquid advancing along the flow path, in operation of the device.

Abstract: Embodiments of the invention are directed to a microfluidic device. The device comprises a flow path structure that includes an inlet microchannel and chambers. The flow path structure is configured as an arborescence extending from the inlet microchannel to the chambers. Thus, liquid introduced in said inlet microchannel can potentially enter the chambers via respective flow paths to remain essentially confined in the chambers, in operation. The device further comprises substances in selected ones of the chambers. That is, a subset of the chambers is loaded with substances adapted for interacting with liquid to yield a detectable change in a property of the liquid and/or the substance in each of the chambers of said subset, in operation. The invention is further directed to related devices, and methods of operation and conditioning.

Abstract: The invention is directed to a microfluidic device, which comprises distinct, parallel levels, including a first level and a second level. It further includes: a first microchannel, a second microchannel, and a node. This node comprises: an inlet port, a cavity, a via, and an outlet port. The cavity is formed on the first level and is open on a top side. The inlet port is defined on the first level; it branches from the first microchannel and communicates with the cavity through an ingress thereof. The outlet port, branches to the second microchannel on the second level. The via extends from the bottom side of the cavity, down to the outlet port, so the cavity may communicate with the outlet port. In addition, the cavity comprises a liquid blocking element to prevent an aqueous liquid filling the inlet port to reach the outlet port.

Abstract: A microfluidic probe head for providing a sequence of separate liquid volumes separated by spacers, the separate liquid volumes including a respective target substance associated with a respective target area, the microfluidic probe head including an inlet and an outlet; a first fluid channel fluidly connected to the inlet, the first fluid channel configured for delivering an injection liquid from the inlet to a respective target area; a second fluid channel fluidly connected to the outlet, the second fluid channel configured for delivering liquid volumes from the respective target area to the outlet; and a spacer insertion unit fluidly connected to the second fluid channel, the spacer insertion unit configured for inserting spacers into the second fluid channel between the liquid volumes to provide the sequence of separate liquid volumes separated by spacers.

Abstract: An assembly is provided for interfacing with a microfluidic chip having at least one microscopic channel configured to receive a liquid sample for analysis. The assembly includes a chip carrier, an electronics module, an optical module, and a mechanical module. The chip carrier includes a base and a cover defining a cavity to receive the microfluidic chip. The electronics module includes a signal generator which applies at least one electrokinetic signal electrode(s) of the chip. The optical module includes an excitation radiation source which causes excitation radiation to impinge on the sample, and an emission radiation detector which detects radiation emitted from the sample. The mechanical module includes a chip-carrier receiving structure, relatable with respect to the optical module for focus and at least one degree of translational freedom.

Abstract: A microfluidic device and method for fabrication includes a microfluidic channel that has a closed portion, which comprises: a liquid pathway formed by a wetting area; and an anti-wetting area extending along and contiguously with the liquid pathway. The anti-wetting area is configured so as to provide a vent to evacuate gas along the anti-wetting area.

Abstract: Methods are provided for producing an authenticated packaged product. The method includes providing on the product a security code encoding security data for the product, packing the product in packaging, and providing a tag carrying encrypted product data on the packaging. The product data comprises the security data for the product. The method further comprises storing a decryption key for the encrypted product data at a network server, and providing on the packaging access data for accessing the decryption key at the network server. Corresponding methods and systems are provided for verifying authenticity of such an authenticated packaged product.

Abstract: Methods are provided for authenticating a container of items to be sent by a sender to a receiver. The method includes: packing a plurality of items, each having a respective item identifier, in the container; providing a container identifier on the container; and generating a first digital signature by signing a message, comprising the container identifier and each item identifier, using a secret signing key skS of a signing-verification key pair (skS, pkS) of a digital signature scheme. The method further comprises providing the first digital signature for access by the receiver, and sending the container to the receiver. A corresponding verification method comprises accessing the first digital signature, and verifying the first digital signature for the container identifier and the item identifier for each item in the container using the verification key pkS of the sender key pair (skS, pkS).

Abstract: A microfluidic device and method for fabrication includes a microfluidic channel that has a closed portion, which comprises: a liquid pathway formed by a wetting area; and an anti-wetting area extending along and contiguously with the liquid pathway. The anti-wetting area is configured so as to provide a vent to evacuate gas along the anti-wetting area.