Abstract: A method of making a photosynthetic bacteria variant which that can produce hydrogen in both day and night. In one embodiment hydrogen is produced in a light independent manner by adding pyruvate lyase and formate lyase complex to a photosynthetic strain of Rhodobacter sphaeroides. A preferred photosynthetic bacteria variant that can produce hydrogen in both day and night from Rhodobacter sphaeroides is KCTC 12085.

Abstract: Disclosed is a method for screening an antioxidant using mutant bacteria and chlorophyllide, and an antioxidant screened by the same method. More specifically, provided is a method for easy screening of an antioxidant by monitoring growth profiles of specific mutant bacteria in filter discs or medium blocks containing chlorophyllide added thereto, and an antioxidant screened by the same method. The present invention enables easy screening of an antioxidant on an industrial scale, by monitoring growth profiles of mutant bacteria via the use of mutant bacteria and chlorophyllide. In particular, the antioxidant screening method in accordance with the present invention is very useful for selective screening of an amphiphilic antioxidant. Therefore, the present invention is applicable to screen and commercialize low-toxic and effective antioxidants which will be used in various food and cosmetic additives as well as therapeutic medicines.