Abstract: The present invention relates to a method of ligating a first peptide via its C-terminus to the N-terminus of a second peptide, wherein the reaction is catalyzed by an asparagine/aspartate (Asx) peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1. Further encompassed are a method of preparing a dimer, oligomer, or multimer of one or more peptides of interest and a method of modifying or tagging the surface of a target cell by one or more peptides of interest. Also encompassed in the invention are the ligated peptides and/or tagged target cells obtainable according to any of the methods, the peptide ligase OaAEPI Cys247Ala having the amino acid sequence of SEQ ID NO: 1, as well as kits comprising said peptide ligase.

Abstract: A data storage method for a data storage device is provided. The data storage method includes steps of: determining whether a power drop/loss event occurs; when the power drop/loss event is determined to have occurred, recording a voltage level of a charge storage device, wherein the charge storage device provides power to the data storage device during the power drop/loss event; determining whether the charge storage device is operating normally according to the recorded voltage level of the charge storage device; and when the charge storage device is determined to be not operating normally, configuring the data storage device to enter a safe operation mode. A data storage device is also provided.

Abstract: Novel compounds are provided that bind to polo-like kinases through the polo-box domain. In certain embodiments, the novel compounds are PEGylated peptides. The PEGylated peptides in accordance with the invention demonstrate high PBD-binding affinity. In certain embodiments, the PEGylated peptides have also achieved activities in whole cell systems. The invention also provides compounds that bind polo-like kinases through the polo-box domain and possess reduced anionic charge. Further provided are methods of design and/or synthesis of the PEGylated peptides and methods of use thereof. The invention provides methods of use of the compounds and methods of synthesis of the compounds.

Abstract: Disclosed herein are insulin agonist peptides conjugated to incretins wherein the incretin-insulin conjugate has agonist activity at the insulin receptor and the corresponding incretin receptor, and stimulates weight loss in an individual administered the compound.

Abstract: The present invention relates to a method of forming a peptide of Formula (I) (P1-Xaa1-Xaa2-P2) by ligating a first peptide of Formula (II) (P1-Xaa1-X—R, wherein X is O or S) to a second peptide of Formula (II I) (Xaa1-Xaa2-P2) by enzymatically cleaving the bond between “Asx” and “X” in the first peptide of Formula (II) and ligating the fragment P1-Asx of the first peptide to the second peptide of Formula (III), wherein the enzymatic cleavage and ligation reaction is catalyzed by butelase 1 (SEQ ID NO: 1) and the peptide of Formula (I) is a depsipeptide, preferably a thiodepsipeptide. Further encompassed are peptides and dendrimeric peptide assemblies prepared using the presently disclosed method, as well as use of the dendrimeric peptide assemblies as a vaccine, medicament, or diagnostic agent, particularly as an antimicrobial agent.

Abstract: A display apparatus including a first substrate, a second substrate opposite to the first substrate, a pixel array disposed between the first substrate and the second substrate and a photoelectric conversion array disposed between the pixel array and the second substrate is provided. The pixel array includes sub-pixel units. An initial light from each of the sub-pixel units is converted into an electrical energy and a display light by the photoelectric conversion array. The electrical energy is stored and the display light passes through the second substrate to display an image. Display lights presented by two adjacent sub-pixel units have different wavelengths. The photoelectric conversion array includes photoelectric conversion cells. An area of each of the photoelectric conversion cells covers the two adjacent sub-pixel units and partially overlaps an area of another photoelectric conversion cell.

Abstract: A data storage method for a data storage device is provided. The data storage method includes steps of: determining whether a power drop/loss event occurs; when the power drop/loss event is determined to have occurred, recording a voltage level of a charge storage device, wherein the charge storage device provides power to the data storage device during the power drop/loss event; determining whether the charge storage device is operating normally according to the recorded voltage level of the charge storage device; and when the charge storage device is determined to be not operating normally, configuring the data storage device to enter a safe operation mode. A data storage device is also provided.

Abstract: A replaceable magnetic screw-locking depth positioning head includes a main body formed by a rod, a positioning sleeve, a buckling ring, a cushioning member and a magnetic member. The positioning sleeve has a stepped slot, so that the buckling ring can be installed into the stepped slot more conveniently, and the stepped slot contains a cushioning member and a magnetic member. The cushioning member provides a buffering/shock absorbing effect and a better manufacture precision of the main body, and the magnetic member provides a better positioning effect for locking the screw, and a first slot is formed at a free end of the stepped slot for accommodating debris. The positioning head of the present invention has the advantages of high manufacturing efficiency, production efficiency and manufacturing precision.

Abstract: Disclosed herein are insulin agonist peptides conjugated to incretins wherein the incretin-insulin conjugate has agonist activity at the insulin receptor and the corresponding incretin receptor, and stimulates weight loss in an individual administered the compound.

Abstract: The invention relates to the field of compounds, especially peptides or polypeptides, that have thrombopoietic activity. The peptides and polypeptides of the invention may be used to increase platelets or platelet precursors (e.g., megakaryocytes) in a mammal.

Abstract: An angled rod for a hand tool comprising a driving part having a driving end for driving a screw, an angled connection part, and a tool-mounting part for connecting to a driving tool. The tool-mounting part is angularly extended to the driving part such that the tool-mounting part and the driving part are non-parallel to each other.

Abstract: A display panel includes an active device array substrate, an opposite substrate, a display medium and a sealant. The active device array substrate includes a substrate, an active device array, a passivation layer and an enhancement layer. A material of the enhancement layer is different from a material of the passivation layer. The opposite substrate is disposed opposite to the active device array substrate. The display medium is disposed between the active device array substrate and the opposite substrate. The sealant is disposed between the active device array substrate and the opposite substrate and surrounds the display medium. An end of the sealant directly contacts the enhancement layer, and a material of the enhancement layer is the same as a material of the sealant.

Abstract: A display apparatus including a first substrate, a second substrate opposite to the first substrate, a pixel array disposed between the first substrate and the second substrate and a photoelectric conversion array disposed between the pixel array and the second substrate is provided. The pixel array includes sub-pixel units. An initial light from each of the sub-pixel units is converted into an electrical energy and a display light by the photoelectric conversion array. The electrical energy is stored and the display light passes through the second substrate to display an image. Display lights presented by two adjacent sub-pixel units have different wavelengths. The photoelectric conversion array includes photoelectric conversion cells. An area of each of the photoelectric conversion cells covers the two adjacent sub-pixel units and partially overlaps an area of another photoelectric conversion cell.

Abstract: The invention relates to the synthesis of peptides, proteins and related bioconjugates, and in particular, to such synthesis using a peptide ligation method whereby a C-terminal salicylaldehyde ester peptide is reacted with an aminoacyl-N-hydroxl peptide. The invention also relates to the synthesis of cyclic peptides, including serinyl- or threonyl-containing cyclic peptides. The invention further relates to a solid phase synthesis of C-terminal salicylaldehyde ester peptides.

Abstract: The present invention is directed to a method of alkylating a thiol group (R—S—H) or seleno group (R—Se—H) in a target molecule wherein the method comprises: reacting a target molecule comprising at least one thiol group with a compound of formula (I) or (II): wherein R is an acetyl group or any other acyl group or is a group comprising any one of: or wherein R in formula (II) can also be an alkyl group; and wherein R? is selected from a group consisting of a hydrogen, a methyl group and an ethyl group. In certain cases, the described methods may be used to install acetylated lysine analogs in target molecules (e.g., peptides, proteins).

Abstract: A making method of a plastic toy with adjustable hardness at local positions is provided, including: a) preparing two kinds of plastic latex with desired hardness; b) making a first gel coat layer, adding one kind of the latex into a mold and heating the mold; c) making a second gel coat layer, adding the other kind of latex; d) gel forming of hard latex and soft latex, adding the first kind of latex and then heating again; e) cooling forming and uncovering demolding, carrying out water cooling and carrying out uncovering demolding after the cooling is completed; and 0 gel processing. The present invention solves the problem and meets the requirements of products about inconsistent hardness of various parts, makes the touch feel of the toys closer to that of the human skin, thus ensuring products having better quality, and bringing better realistic feel of the toy to people.

Abstract: A frit encapsulation apparatus includes a carriage, a mask, a laser light source and a pressure element. The carriage is disposed over a first substrate. The mask is disposed in the carriage and has a light-transmitting region. The laser light source is disposed in the carriage and over the mask and is configured to provide laser light through the light-transmitting region of the mask and the first substrate therebeneath to heat the frit beneath the first substrate. The pressure element is disposed beneath the carriage and is configured to provide a pressure to the first substrate, such that the first substrate is adhered to a second substrate by the heated frit, in which the pressure element is not overlapped with a vertical projection of the light-transmitting region on the first substrate.

Abstract: The invention relates to the synthesis of peptides, proteins and related bioconjugates, and in particular, to such synthesis using a peptide ligation method whereby a C-terminal salicylaldehyde ester peptide is reacted with an aminoacyl-N-hydroxl peptide. The invention also relates to the synthesis of cyclic peptides, including serinyl- or threonyl-containing cyclic peptides. The invention further relates to a solid phase synthesis of C-terminal salicylaldehyde ester peptides.