Abstract: It is intended to provide an antibody specific to HbA1c, antibody-producing cells capable of supplying the antibody in a stable state in the future, and a method of constructing the antibody-producing cells without any probability factors, and a method which comprises fusing mouse spleen cells, which have been sensitized with an immunogen composed of a compound containing the following structural formula (I) and a binding protein, with a myeloma-origin cell line, obtaining monoclonal antibody-producing cells by cloning, and then purifying and acquiring the monoclonal antibody produced by these cells into the culture supernatant.

Abstract: A biosensor according to the present invention includes a reagent immobilization part (5) where an antibody for a measurement target in an inspection target solution is immobilized, and a marked reagent holding part (4) where an antibody which is marked at a part of a development layer in a dry state to be eluted by development of the inspection target solution is held, at parts of the development layer for developing the inspection target solution, and measures a bonding amount of the marked reagent bonded in the reagent immobilization part (5), thereby qualitatively and quantitatively measuring a measurement component in the inspection target solution. In such bionsensor, side faces of the development layer which are parallel to the direction of the inspection target solution permeating are partially or entirely melted and cured to be sealed.

Abstract: The present invention provides an extracorporeal diagnostic used for measurement of a substance to be tested in a specimen. The extracorporeal diagnostic comprises a reagent which specifically binds to the substance to be tested, and a hydrophilic material (a sugar or a sugar derivative). More particularly, the present invention relates to an extracorporeal diagnostic for measuring a substance to be tested in a specimen. The extracorporeal diagnostic comprises 1) a reagent which specifically binds to the substance to be tested and 2) a compound comprising at least one hydroxyl group and at least one aldehyde group or ketone group.

Abstract: To enable accurate qualitative and quantitative analyses of an analyte in a sample without being influenced by a prozone phenomenon, there is disclosed a specific binding method including the steps of: bringing into contact a sample with a zone where a first specific binding substance labeled with a labeling material is retained, to allow an analyte to bind to the first specific binding substance; bringing into contact the sample with a first detection zone where a second specific binding substance is immobilized, to allow an excess of the analyte to bind to the second specific binding substance; bringing into contact the sample with a second detection zone where a substance identical to the analyte is immobilized, to allow the first specific binding substance to bind to the substance; respectively measuring the intensities of signal 1 and signal 2 obtained in the first detection zone and the second detection zone and attributed to the labeling material; and then determining the concentration of the analyte b

Abstract: In a chromatography measuring device according to the present invention, a region at least from a marker reagent holding part 3 in which a marker reagent is held to a specific protein immobilization part 5 in which a color reaction is seen in a chromatography specimen 1 is adherently covered with a liquid-impermeable sheet material 6 composed of plastic tape or the like, and other regions are not covered but opened, as shown in FIG. 1.

Abstract: A bio-device includes a sample application section; an indicator substance holding section; and a determination section. The sample application section, the indicator substance holding section, and the determination section are located so that a liquid sample applied to the sample application section is transferred to the determination section via the indicator substance holding section. At least the indicator substance holding section and the determination section are included in a single member. The indicator substance holding section has a first substance group containing a substance specifically reacting with a target substance, wherein the first substance group is held so as to be capable of being eluted by the applied liquid sample. After the first substance group is eluted by the liquid sample applied to the sample application section, the first substance group flows as a mass having a leading end and a trailing end during flowing.

Abstract: As shown in FIG. 1, a biosensor according to the present invention is provided with an area in which a reagent having bleaching action is carried in a state where it can be dissolved, at least in a part of a sample application area to which an inspection target solution is applied or the downstream of the sample application area in the direction of the inspection target solution permeating, in a development layer.

Abstract: An immunochromatographic device includes a sample application section; a determination section; and a capillary-flow section. The determination section has an immobilization section including one type of first antibody specifically reacting with all the types of target substances, or a plurality of types of first antibodies each specifically reacting with a corresponding type of target substance, the one type of first antibody or the plurality of types of first antibodies being immobilized. The sample application section or the capillary-flow section has a plurality of types of second antibodies each specifically reacting with all the types of target substances or a corresponding type of target substance. The plurality of types of second antibodies can be eluted. At least one type of second antibody specifically reacts with one type of target substance among the plurality of types of target substances. The plurality of types of second antibodies are labeled with different labeling substances.

Abstract: According to the biosensor and the blood component analytical method of the present invention, in a biosensor that is made of a single layer or plural layers of a porous material as shown in FIG. 1, having a reagent holding part and utilizing chromatography, a cell shrinkage reagent is carried on at least part of the reagent holding part, or at least part of a chromatographically developed part that is upstream of the reagent holding part.

Abstract: A biosensor which is provided with a development layer (2) for developing an inspection target solution, that has a reagent immobilization part (5) where an antibody for a measurement target in the inspection target solution is immobilized and a reagent holding part (4) which marks in a dry state and holds an antibody which can be eluted by development of the inspection target solution in parts of the development layer (2), and measures a bonding amount of the marker reagent bonded to the reagent immobilization part (5), thereby qualitatively or quantitatively measuring components to be measured in the inspection target solution, is further provided with a space forming material (8) that forms a cavity part (1), which is a space into which the inspection target solution flows, between the development layer (2) for developing the inspection target solution and the space forming material (8).

Abstract: A method of the present invention is a fluorescence polarization method at multiple wavelengths for analyzing two or more different assay-objects in a sample. The method includes the steps of: (a) providing two or more different fluorescent-labeled substances, each being a substance which is capable of specifically binding to respective one of the assay-objects and is covalently bound to a fluorochrome, wherein the fluorochromes of the fluorescent-labeled substances are different from one another; (b) allowing the fluorescent-labeled substances to bind to the two or more different assay-objects, respectively; and (c) measuring a change in the degree of fluorescence polarization which has taken place in each of the fluorescent-labeled substances by its binding to one of the assay-objects.