Abstract: The present invention relates to a scalable multiplex PCR method that can simultaneously amplify overlapping amplicons without the drawbacks of conventional multiplex PCR. The method selectively amplifying target nucleic acid fragments having an overlapping region. The method comprises the steps of: obtaining a first nucleic acid sequence comprising a first tag t2 and a first forward primer F1, obtaining a second nucleic acid sequence comprising a second tag t1 and a first reverse primer R1, obtaining a third nucleic acid sequence comprising the second tag t1 and a second forward primer F2, obtaining a fourth nucleic acid sequence comprising a third tag 3 and a second reverse primer R2, wherein each primer is a gene-specific primer; performing initial cycles of PCR; and then performing later cycles of PCR at higher annealing temperatures to obtain amplification products.

Abstract: A gas shielded triple-wire indirect arc welding device has three welding wires and the two arc power supplies. In a gas shielded triple-wire indirect arc welding method, before welding, one of three welding wires is first connected to positive electrodes of a first arc power supply and a second arc power supply, the other two welding wires are respectively connected to negative electrodes of the first arc power supply and the second arc power supply, and a welding workpiece is not connected to the arc power supplies. The welding wire connected to the positive electrodes of the two arc power supplies are arranged in the middle, and the other two welding wires are respectively arranged on both sides. The welding method is used for implementing build-up welding.

Abstract: A memory fault detection method includes: receiving a first interrupt signal sent when a count value of a first leaky bucket counter of a server reaches a first threshold; disabling an interrupt switch of the first leaky bucket counter; enabling the interrupt switch of the first leaky bucket counter after the interrupt switch of the first leaky bucket counter has been disabled for a preset time and the count value of the first leaky bucket counter is reset to zero; receiving a second interrupt signal sent when a count value of a second leaky bucket counter reaches a second threshold; if the second leaky bucket counter and the first leaky bucket counter are a same leaky bucket counter, and the second rank and a first rank are a same rank, determining that a hardware fault occurs in the first rank.

Abstract: The invention includes a chimeric antigen receptor (CAR) specific for a natural-killer group 2, member D ligand (NKG2DL), compositions comprising the CAR, polynucleotides encoding the CAR, vectors comprising a polynucleotide encoding the CAR, and recombinant T cells comprising the CAR. The invention also includes methods of making a genetically modified T cell expressing a NKG2D-CAR wherein the expressed CAR comprises a NKG2D extracellular domain. The present invention also relates generally to the use of T cells engineered to express a CAR to treat cancer associated with NKG2D.

Abstract: The present invention relates to a scalable multiplex PCR method that can simultaneously amplify overlapping amplicons without the drawbacks of conventional multiplex PCR. The method selectively amplifying target nucleic acid fragments having an overlapping region. The method comprises the steps of: obtaining a first nucleic acid sequence comprising a first tag t2 and a first forward primer F1, obtaining a second nucleic acid sequence comprising a second tag t1 and a first reverse primer R1, obtaining a third nucleic acid sequence comprising the second tag t1 and a second forward primer F2, obtaining a fourth nucleic acid sequence comprising a third tag t3 and a second reverse primer R2, wherein each primer is a gene-specific primer; performing initial cycles of PCR; and then performing later cycles of PCR at higher annealing temperatures to obtain amplification products.

Abstract: The present invention provides a virulent Pseudomonas fluorescens (P. fluorescens) phage ?Pf1901, and a phage ?Pf1901 preparation and an application thereof, and relates to the technical field of phage. The virulent P. fluorescens phage ?Pf1901 has an accession number of CCTCC M2019447. The virulent phage ?Pf1901 has a titer of (1.4-3)×1010 PFU/mL. The virulent phage ?Pf1901 has an optimal multiplicity of infection (MOI) value of 0.0001. The virulent P. fluorescens phage ?Pf1901 provided by the present invention exhibits very high specificity and lytic ability to P. fluorescens, which can be used to control P. fluorescens, with strong lytic and scavenging effects on a host.

Abstract: In a fault processing method, when it is determined that a computer crashes, a baseboard management controller in the computer can send a read request message to a processor in the computer, where the read request message is used for requesting reading of first error data recorded by the processor, receive a read response message returned by the processor, and obtain, according to the read response message, the first error data recorded by the processor.

Abstract: The present invention provides an operation recognition apparatus and method, and a smart terminal having the device. The operation recognition apparatus comprises an operation receiving unit, a scanning array composed of at least one driving channel and at least one sampling channel, and at least one signal reading unit; the driving channels and the sampling channels intersect to form at least one electrical node, and the signal reading units are connected with all the sampling channels and configured to acquire electric signals on all the sampling channels; the signal sources are connected with the respective driving channels and send electric signals to all the driving channels at the same time, wherein the electric signals of at least two driving channels are at different frequencies; and each signal reading unit comprises at least one band-pass filter for acquiring the electric signals of each sampling channel.

Abstract: Implementations relate to causing a command to be executed based on an image. In some implementations, a computer-implemented method includes obtaining and programmatically analyzing an image to determine suggested actions. The method causes a user interface to be displayed that includes user interface elements corresponding to default actions, and to suggested actions that are determined based on analyzing the image. The method receives user input indicative of selection of a particular action from the default actions and the suggested actions. The method causes a command to be executed by a computing device for the particular action that was selected.

Abstract: The present invention provides a virulent Pseudomonas fluorescens (P. fluorescens) phage ?Pf1901, and a phage ?Pf1901 preparation and an application thereof, and relates to the technical field of phage. The virulent P. fluorescens phage ?Pf1901 has an accession number of CCTCC M2019447. The virulent phage ?Pf1901 has a titer of (1.4?3)×1010 PFU/mL. The virulent phage ?Pf1901 has an optimal multiplicity of infection (MOI) value of 0.0001. The virulent P. fluorescens phage ?Pf1901 provided by the present invention exhibits very high specificity and lytic ability to P. fluorescens, which can be used to control P. fluorescens, with strong lytic and scavenging effects on a host.

Abstract: Implementations relate to removal of one or more images from a view of a plurality of images. In some implementations, a method includes obtaining a plurality of images, programmatically analyzing the plurality of images to determine a plurality of image features, and determining one or more image categories for the plurality of images based on the image features. The method further includes identifying a subset of the plurality of images based on the image categories, wherein each image of the subset is associated with an image category for archival. The method further includes causing a user interface to be displayed that includes one or more images of the subset, receiving user input to archive at least one of the one or more images, and in response to the user input, removing the at least one of the images from a view of the plurality of images.

Abstract: Implementations relate to causing a command to be executed based on an image. In some implementations, a computer-implemented method includes obtaining and programmatically analyzing an image to determine suggested actions. The method causes a user interface to be displayed that includes user interface elements corresponding to default actions, and to suggested actions that are determined based on analyzing the image. The method receives user input indicative of selection of a particular action from the default actions and the suggested actions. The method causes a command to be executed by a computing device for the particular action that was selected.

Abstract: A memory fault detection method includes: receiving a first interrupt signal sent when a count value of a first leaky bucket counter of a server reaches a first threshold; disabling an interrupt switch of the first leaky bucket counter; enabling the interrupt switch of the first leaky bucket counter after the interrupt switch of the first leaky bucket counter has been disabled for a preset time and the count value of the first leaky bucket counter is reset to zero; receiving a second interrupt signal sent when a count value of a second leaky bucket counter reaches a second threshold; if the second leaky bucket counter and the first leaky bucket counter are a same leaky bucket counter, and the second rank and a first rank are a same rank, determining that a hardware fault occurs in the first rank.

Abstract: Disclosed is an optical device (10) comprising a plurality of lenses (100) for projecting a plurality of parallel beams (202, 204) having a predetermined width towards an object area, wherein each lens comprises a lens body including a cavity (150) for housing a solid state lighting element (200); a pair of opposing internally reflecting side surfaces (130) for constraining said beam within said predetermined width; and a light exit surface delimited by said opposing side surfaces, the light exit surface comprising a curved region (110) extending from a first further side surface (141) extending between the opposing side surfaces and shaped to generate a collimated beam portion along an optical axis; and a stepped region in between the curved region and a second further side surface (142) extending between the reflecting side surfaces, wherein said steps are defined by a plurality of prismatic protrusions (120), each of said protrusions laterally extending between said opposing side surfaces.

Abstract: A fault processing method, a related apparatus, and a computer. When it is determined that a computer crashes, a baseboard management controller in the computer can send a read request message to a processor in the computer, where the read request message is used for requesting reading of error data recorded by the processor, receive a read response message returned by the processor, and obtain, according to the read response message, the error data recorded by the processor. By means of the embodiments of the present invention, an operating system does not need to be used, acquisition of error data in a computer after the computer crashes is implemented using a baseboard management controller, and a problem in the prior art that error data in a computer cannot be acquired after a severe uncorrectable error occurring in the computer causes a system crash is resolved.

Abstract: The present invention discloses an optical device (100) and a lighting device for illuminating two intersecting surfaces (S1, S2). The optical device (100) comprises a first light guiding structure (120) for redirecting light from a light source (200) into a first direction and a second light guiding structure (130) for redirecting light from the light source (200) into a second direction different from the first direction, wherein the optical device (100) is arranged to prevent light from the light source (200) from being redirected in a direction between the first and second directions. By positioning the lighting device (100) at the intersection of two surfaces (S1, S2) such as can be found in a step, or at a corner of a wall, or at a corner inside a closet, or on the intersection area of ceilings, the two intersecting surfaces can be illuminated at the same time with comfortable light distribution, using a simple structure that can be easily installed.

Abstract: A troubleshooting method implemented by a processor device is provided, comprising determining, according to collected information of correctable errors, that a correctable error storm has occurred, disabling a system management interrupt (SMI) of generation modules of correctable errors in a correctable error set, wherein the correctable error set comprises correctable errors related to the correctable error storm, sending SMI-disabled notification information to a baseboard management controller (BMC), receiving enable-SMI notification information that is sent by the BMC after a predetermined time elapses after the SMI-disabled notification information has been received, and enabling the disabled SMI of the generation modules of the correctable errors according to the enable-SMI notification information.

Abstract: The present application relates to the technical field of luminaire, and provides a track head for mounting a lamp body on a track strip, the lamp body comprises a mounting rack, the track head comprises a cassette body fixed to the mounting rack and a cover body movably mounted on the track strip, the cover body is detachably mounted on the cassette body, a plurality of first resilient sheets which are electrically connected to the lamp body are provided in the inner part of the cassette body, a plurality of second resilient sheets which are electrically connected to the first resilient sheets respectively are provided in the inner side of the cover body, a conductive structure is provided between the cover body and the track strip for electrical interconnection with each other.

Abstract: The present invention relates to a scalable multiplex PCR method that can simultaneously amplify overlapping amplicons without the drawbacks of conventional multiplex PCR. The method selectively amplifying target nucleic acid fragments having an overlapping region. The method comprises the steps of: obtaining a first nucleic acid sequence comprising a first tag t2 and a first forward primer F1, obtaining a second nucleic acid sequence comprising a second tag t1 and a first reverse primer R1, obtaining a third nucleic acid sequence comprising the second tag t1 and a second forward primer F2, obtaining a fourth nucleic acid sequence comprising a third tag t3 and a second reverse primer R2, wherein each primer is a gene-specific primer; performing initial cycles of PCR; and then performing later cycles of PCR at higher annealing temperatures to obtain amplification products.