Abstract: The present invention relates to a scalable multiplex PCR method that can simultaneously amplify overlapping amplicons without the drawbacks of conventional multiplex PCR. The method selectively amplifying target nucleic acid fragments having an overlapping region. The method comprises the steps of: obtaining a first nucleic acid sequence comprising a first tag t2 and a first forward primer F1, obtaining a second nucleic acid sequence comprising a second tag t1 and a first reverse primer R1, obtaining a third nucleic acid sequence comprising the second tag t1 and a second forward primer F2, obtaining a fourth nucleic acid sequence comprising a third tag t3 and a second reverse primer R2, wherein each primer is a gene-specific primer; performing initial cycles of PCR; and then performing later cycles of PCR at higher annealing temperatures to obtain amplification products.

Abstract: A fault processing method, a related apparatus, and a computer. When it is determined that a computer crashes, a baseboard management controller in the computer can send a read request message to a processor in the computer, where the read request message is used for requesting reading of first error data recorded by the processor, receive a read response message returned by the processor, and obtain, according to the read response message, the first error data recorded by the processor. By means of the embodiments of the present invention, an operating system does not need to be used, acquisition of error data in a computer after the computer crashes is implemented using a baseboard management controller, and a problem in the prior art that error data in a computer cannot be acquired after a severe uncorrectable error occurring in the computer causes a system crash is resolved.

Abstract: The present invention reduces primer-dimer amplification in a multiplex polymerase chain reaction (PCR). When a first forward primer (F1) and a second reverse primer (R2) have a complementary region at their 3?ends, primer dimers may form. The present method uses a primer comprising a 5?-end partial sequence or a full sequence of a first forward primer (F1^) in between a first tag (t1) and R2 to reduce the primer-dimer (F1_R2) amplification.

Abstract: The invention discloses an optical assembly comprising a plurality of optical components that are concentric to one another, each optical component having a partially reflective and partially transmissive interface with an oblique angle of incidence, and the reflectivity of the interface increasing with distance from a common center; a transitional optical component is provided between the light source and the first optical component in order to direct lights from a light source to the partially reflective and partially transmissive interface of the first optical component. By assembling the plurality of optical components, a much thinner lighting device (100) can be achieved.

Abstract: The present invention reduces primer-dimer amplification in a multiplex polymerase chain reaction (PCR). When a first forward primer (F1) and a second reverse primer (R2) have a complementary region at their 3?ends, primer dimers may form. The present method uses a primer comprising a 5?-end partial sequence or a full sequence of a first forward primer (F1?) in between a first tag (t1) and R2 to reduce the primer-dimer (F1_R2) amplification.

Abstract: Disclosed is an optical device (10) comprising a plurality of lenses (100) for projecting a plurality of parallel beams (202, 204) having a predetermined width towards an object area, wherein each lens comprises a lens body including a cavity (150) for housing a solid state lighting element (200); a pair of opposing internally reflecting side surfaces (130) for constraining said beam within said predetermined width; and a light exit surface delimited by said opposing side surfaces, the light exit surface comprising a curved region (110) extending from a first further side surface (141) extending between the opposing side surfaces and shaped to generate a collimated beam portion along an optical axis; and a stepped region in between the curved region and a second further side surface (142) extending between the reflecting side surfaces, wherein said steps are defined by a plurality of prismatic protrusions (120), each of said protrusions laterally extending between said opposing side surfaces.

Abstract: Embodiments of the present invention disclose a touch control responding method and apparatus, where in the touch control responding method, after a first touch control interrupt instruction is received, first responding to the first touch control interrupt instruction is delayed, and whether a second touch control interrupt instruction is generated is determined within a preset delay time; if the second touch control interrupt instruction is generated, it indicates that a touch control operation is performed by a user in a middle area between a touch control button and a touch screen, and in this case, a first control instruction responding only to the first touch control interrupt instruction or the second touch control interrupt instruction is generated.

Abstract: A luminaire (100) comprising a chamber (110) comprising at least one light exit surface (112); an axial carrier (120) mounted in said chamber (110) on an axis (105), said axial carrier (120) carrying a plurality of solid state lighting elements (122) and being surrounded by the light exit surface (112); and a body (130) mounted around said axial carrier (120), said body (130) comprising a plurality of radially extending optical cells (140) each comprising an inlet (142) facing said axial carrier (120); an outlet (144) facing the light exit surface (112); and a plurality of reflective surfaces (146, 148) extending from said inlet (142) to said outlet (144); wherein at least one of the axial carrier (120) and the body (130) are rotatably mounted relative to said axis (105) and wherein the body (130) can be rotated relative to the axcial carrier (120) or vice versa.

Abstract: Disclosed is a luminaire (100) comprising a chamber delimited by a body (110) defining a light exit surface of the luminaire; and an optical cavity (135) inside the chamber such that the chamber comprises a volume (112) delimited by the body and the optical cavity, wherein the optical cavity is delimited by a first parabolic mirror (120) and a second parabolic mirror (130) facing the first parabolic mirror, the second parabolic mirror comprising an aperture (132) facing the volume, the optical cavity comprising a light source (140) mounted inside said cavity; wherein the first parabolic mirror and the second parabolic mirror are shaped to create an image (12) of the light source in the volume.

Abstract: The present invention relates to a scalable multiplex PCR method that can simultaneously amplify overlapping amplicons without the drawbacks of conventional multiplex PCR. The method selectively amplifying target nucleic acid fragments having an overlapping region. The method comprises the steps of: obtaining a first nucleic acid sequence comprising a first tag t2 and a first forward primer F1, obtaining a second nucleic acid sequence comprising a second tag t1 and a first reverse primer R1, obtaining a third nucleic acid sequence comprising the second tag t1 and a second forward primer F2, obtaining a fourth nucleic acid sequence comprising a third tag t3 and a second reverse primer R2, wherein each primer is a gene-specific primer; performing initial cycles of PCR; and then performing later cycles of PCR at higher annealing temperatures to obtain amplification products.

Abstract: The invention discloses an optical assembly comprising a plurality of optical components that are concentric to one another, each optical component having a partially reflective and partially transmissive interface with an oblique angle of incidence, and the reflectivity of the interface increasing with distance from a common center; a transitional optical component is provided between the light source and the first optical component in order to direct lights from a light source to the partially reflective and partially transmissive interface of the first optical component. By assembling the plurality of optical components, a much thinner lighting device (100) can be achieved.

Abstract: Embodiments of the present invention relate to a charging method, a mobile device, and a charging device. The mobile device includes a battery and a connector, where the connector includes a charging pin and a ground pin. When the charging pin establishes a connection to an output pin of the charging device by using a charging cable or a battery charging stand, the charging pin receives a first current signal transmitted by the output pin of the charging device, and transmits the first current signal to an anode pin of a battery, so as to charge the battery.

Abstract: An entrainment-based mechanical ventilator may be initially set to approximate the patient's spontaneous respiratory frequency, enabling the patient to entrain to the ventilator if the ventilator frequency matches the patient's spontaneous breathing frequency or is within a reasonable range from it. If the preset frequency of the ventilator is set too high or too low from the spontaneous frequency, the patient will fall out of phase and “fight” the ventilator. The ventilator phase relative to the patient's breathing effort indicates the difference between the ventilator frequency and spontaneous frequency. Based on the phase difference, a closed-loop control mechanism may continuously adjust the ventilator frequency to match the patient breathing frequency until the phase shift is within a preset limit. Patient-ventilator entrainment will occur when the ventilator frequency is within a certain range from the patient breathing frequency.

Abstract: A low-power laser and arc hybrid welding method includes the steps of matching laser pulses with arc phases, and inducing compress arcs by the laser pulses. A laser peak pulse is triggered from half of the positive half-wave to half of the negative half-wave of alternating arc current. The sum of laser peak pulse width and laser basic pulse width is equal to the time width from a laser pulse triggered point to a negative half-wave end point of the alternating arc current. A welding device for carrying out the method is disclosed. An angle formed by the axis of a laser beam (1) and the vertical direction is in the range of ?50°-50°. An angle formed by the axis of the laser beam (1) and the axis of an arc torch (2) is in the range of 20°-120°. Arc power density is adjusted minutely in the range of 103-105 by adjusting the alternating arc current, protrusion quantity of an electrode (3) and gas flow velocity of a nozzle. The method and the device can reduce the consumption and save the cost.

Abstract: An identification information module is connected to a non-power pin inside a USB charger charging interface, and the identification information module contains identification information that marks identity information of the USB charger, and meanwhile, an identification information acquisition module is set between the non-power pin and a charging control circuit, configured to acquire the identification information inside the USB charger and provide it to the charging control circuit, and the charging control circuit compares the acquired identification information with standard identification information, and if the identification information is within the standard information range, the hand held device is capable of using the USB charger for charging; otherwise, the hand held device is not capable of using the USB charger for charging, thereby ensuring the charging safety of the hand held device.

Abstract: A low-power laser and arc hybrid welding method includes the steps of matching laser pulses with arc phases, and inducing compress arcs by the laser pulses. A laser peak pulse is triggered from half of the positive half-wave to half of the negative half-wave of alternating arc current. The sum of laser peak pulse width and laser basic pulse width is equal to the time width from a laser pulse triggered point to a negative half-wave end point of the alternating arc current. A welding device for carrying out the method is disclosed. An angle formed by the axis of a laser beam (1) and the vertical direction is in the range of ?50°-50°. An angle formed by the axis of the laser beam (1) and the axis of an arc torch (2) is in the range of 20°-120 °. Arc power density is adjusted minutely in the range of 103-105 by adjusting the alternating arc current, protrusion quantity of an electrode (3) and gas flow velocity of a nozzle. The method and the device can reduce the consumption and save the cost.

Abstract: A method for controlling the air-fuel ratio of an internal combustion engine operating with alternating intake valves is presented. According to the method, engine air-fuel ratio can be adjusted by changing valve timings or fuel during a cylinder cycle.

Abstract: A method for controlling the air-fuel ratio of an internal combustion engine operating with alternating intake valves is presented. According to the method, engine air-fuel ratio can be adjusted by changing valve timings or fuel during a cylinder cycle.

Abstract: A method for controlling the air-fuel ratio of an internal combustion engine operating with alternating intake valves is presented. According to the method, engine air-fuel ratio can be adjusted by changing valve timings or fuel during a cylinder cycle.

Abstract: An entrainment-based mechanical ventilator may be initially set to approximate the patient's spontaneous respiratory frequency, enabling the patient to entrain to the ventilator if the ventilator frequency matches the patient's spontaneous breathing frequency or is within a reasonable range from it. If the preset frequency of the ventilator is set too high or too low from the spontaneous frequency, the patient will fall out of phase and “fight” the ventilator. The ventilator phase relative to the patient's breathing effort indicates the difference between the ventilator frequency and spontaneous frequency. Based on the phase difference, a closed-loop control mechanism may continuously adjust the ventilator frequency to match the patient breathing frequency until the phase shift is within a preset limit. Patient-ventilator entrainment will occur when the ventilator frequency is within a certain range from the patient breathing frequency.