Abstract: The invention concerns methods for the detection of an analyte in a sample by chemiluminescence or electrochemiluminescence using derivatized test reagents. In addition new reagents and reagent kits for chemiluminescence and electrochemiluminescence detection methods are disclosed.

Abstract: The invention concerns rhodamine derivatives of the general formulae in which Ca-Cd each denote a C atom, and Ca and Cb as well as Cc and Cd are either linked together by a single bond or by a double bond; X1 to X16 denote independently of one another halogen, sulfonic acid, hydrogen or an alkyl residue with 1-20 C atoms in which the alkyl residue can be substituted with one or several halogen or sulfonic acid residues; R1 and R2 are either identical or different and denote either hydrogen, alkyl with 1-20 C atoms, polyoxyhydrocarbyl units, phenyl or phenylalkyl with 1-3 carbon atoms in the alkyl chain in which the alkyl and/or phenyl residues can be substituted by one or several hydroxy, halogen, sulfonic acid, amino, carboxy or alkoxycarbonyl groups where alkoxy can have 1-4 carbon atoms, R1 contains at least one activatable group, R2 and X4 can be optionally linked together via a bridge composed of 0-2 C atoms.

Abstract: Compounds of the general formula Ia and Ib are described ##STR1## in which R.sup.1 and R.sup.2 are the same or different and represent hydrogen, straight-chain or branched C.sub.1 to C.sub.6 lower alkyl or an aryl group optionally substituted by electron-withdrawing groups, R.sup.3 denotes a cleavable group, W is hydrogen, halogen or a pseudohalogen and at least one of the groups R.sup.4 or R.sup.5 is a group stabilizing the dioxetane structure and at most one of the groups R.sup.4 or R.sup.5 represents hydrogen and X or Y represents oxygen, N-R or C(R).sub.2 in which R has the meanings stated for R.sup.1 and R.sup.2 or represents a mesomeric double bond or a carbonyl group and n denotes the number 0 or 1 and m denotes the number 1 or 2, as well as a process for their production. These compounds are new and can be used as substrates in immunological assays and in DNA diagnostics using activating agents for colour formation.

Abstract: The invention concerns the use of redox-active compounds for the production of detection reagents for a method for the determination of an analyte as well as reagent kits that contain these redox-active compounds. In addition new redox-active compounds are disclosed.

Abstract: Compounds of the general formula III, ##STR1## in which R represents ##STR2## and in which R.sub.1 and R.sub.2 are the same or different and represent hydrogen or straight-chained or branched lower alkyl C.sub.1 - C.sub.6, and X represents a cleavable group, at least one of the groups R.sub.4 or R.sub.5 represents a group that stabilizes the dioxetan structure and at most one of the groups R.sub.4 or R.sub.5 represents hydrogen. These compounds can be used as substrates in immunological assays and in DNA diagnostics using activating agents for the formation of colour.

Abstract: The present invention concerns new hydrophilic metal complexes and their use as luminescent marker groups in immunoassays.

Abstract: The present invention concerns new metal complexes with a charged linker and their use as luminescent marker groups in an immunoassay.

Abstract: The present invention concerns a method for the determination of an analyte in a sample liquid using a metal complex capable of luminescence as an analyte-specific marker group for the production of a measuring signal in which an unspecific metal complex is additionally added as an interference elimination reagent which has a structure that is chemically related to the marker group.

Abstract: Pentacyclic derivatives having the general formulae (Ia), (Ib) and (Ic) ##STR1## denote: hydrogen, alkyl with 1 to 20 carbon atoms polyoxyhydrocarbyl, phenyl, phenylalkyl with 1 to 3 carbon atoms in the alkyl chain, wherein the alkyl residues or/and phenyl residues can be substituted by one or several hydroxy, halogen, sulfo, carboxy or alkoxycarbonyl groups in which alkoxy can have 1 to 4 carbon atoms;R.sup.7 denotes an alkyl group with 1 to 7 carbon atoms, substituted by at least one halogen, or denotes a carboxyalkyl group or a phenyl group which is substituted by a carboxy or alkoxycarbonyl group located at the o-position relative to the carbon atom bound to the pentacyclic ring system and by at least one halogen, wherein alkoxy can have 1 to 4 carbon atoms, or a carboxymethylene-oxy-alkyloxy group; the residues R.sup.14 and R.sup.

Abstract: Compounds of the general formula III, ##STR1## in which R represents ##STR2## and in which R.sub.1 and R.sub.2 are the same or different and represent hydrogen or straight-chained or branched lower alkyl C.sub.1 -C.sub.6, and X represents a cleavable group, at least one of the groups R.sub.4 or R.sub.5 represents a group that stabilizes the dioxetan structure and at most one of the groups R.sub.4 or R.sub.5 represents hydrogen. These compounds can be used as substrates in immunological assays and in DNA diagnostics using activating agents for the formation of colour.

Abstract: Benzodiazepine protein conjugates of the formula I ##STR1## wherein R1 is hydrogen, a methyl group or R, R2 is hydrogen, a hydroxyl or an OR group, with R1 or R2 containing an R group,R3 is a halogen, NO2 or NH2,X is hydrogen or a halogen,wherein R is a group of the formula II, wherein Z is a macromolecular immunogen reactive carrier substance and n is 2 or 3.The benzodiazepine protein conjugates are used as immunogens to obtain antibodies to benzodiazepines.The invention also relates to an immunoassay for the detection of benzodiazepines where the so generated antibodies are used.

Abstract: Compounds of the general formula III, ##STR1## in which R represents ##STR2## and in which R.sub.1 and R.sub.2 are the same or different and represent hydrogen or straight-chained or branched lower alkyl C.sub.1 -C.sub.6, and X represents a cleavable group, at least one of the groups R.sub.4 or R.sub.5 represents a group that stabilizes the dioxetan structure and at most one of the groups R.sub.4 or R.sub.5 represents hydrogen. These compounds can be used as substrates in immunological assays and in DNA diagnostics using activating agents for the formation of colour.