Patents by Inventor Haruhiko Yamaguchi

Haruhiko Yamaguchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240174989
    Abstract: The present disclosure aims to provide a method which, even when a protein complex is formed, can purify a protein derived from such a membrane protein complex by eluting the membrane protein complex maintaining its complex form. The present disclosure relates to a method for purifying a protein derived from a membrane protein complex, which includes: a protein binding step of binding to an antibody a membrane protein complex eluted from a membrane using a synthetic polymer; a protein eluting step of eluting the membrane protein complex bound to the antibody in the protein binding step at a pH of 6.0 or higher; and a synthetic polymer removing step of subjecting the membrane protein complex eluted in the protein eluting step to solvent extraction at a pH of 7.0 or lower to remove the synthetic polymer.
    Type: Application
    Filed: March 23, 2022
    Publication date: May 30, 2024
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASHI, Toru NAKAYAMA
  • Publication number: 20230279370
    Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CET) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles, and mutating the amino acid sequence of a C-terminal region of the cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
    Type: Application
    Filed: February 6, 2023
    Publication date: September 7, 2023
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASHI, Toru NAKAYAMA, Satoshi YAMASHITA, Yuzuru TOZAWA
  • Publication number: 20230279369
    Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
    Type: Application
    Filed: February 6, 2023
    Publication date: September 7, 2023
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASHI, Toru NAKAYAMA, Satoshi YAMASHITA, Yuzuru TOZAWA
  • Publication number: 20230279371
    Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
    Type: Application
    Filed: February 6, 2023
    Publication date: September 7, 2023
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASHI, Toru NAKAYAMA, Satoshi YAMASHITA, Yuzuru TOZAWA
  • Publication number: 20230167465
    Abstract: The present disclosure provides a method for producing a polyisoprenoid, which makes it possible to synthesize in vitro a polyisoprenoid having an unprecedented structure, such as a 100% cis-polyisoprenoid or a polyisoprenoid containing an allylic diphosphate derivative as an initiating terminal. The present disclosure relates to a method for producing a polyisoprenoid in vitro, which employs a gene coding for a neryl diphosphate synthase and rubber particles bound to a protein encoded by the gene, or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a gene coding for a neryl diphosphate synthase is linked to a promoter having a promoter activity that drives laticifer-specific gene expression to express a protein encoded by the gene specifically in laticifers.
    Type: Application
    Filed: February 22, 2021
    Publication date: June 1, 2023
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Kazuhisa FUSHIHARA, Seiji TAKAHASHI, Toru NAKAYAMA, Satoshi YAMASHITA
  • Publication number: 20230002792
    Abstract: Objects are to provide: a fusion protein capable of binding to lipid droplets while having an enzymatic activity to synthesize a hydrophobic compound; a method for producing a substance including accumulating a hydrophobic compound in lipid droplets using the fusion protein; a vector which can enhance production of a hydrophobic compound when it is introduced into cells using genetic recombination techniques; and a transgenic cell into which the vector or a gene coding for the fusion protein has been introduced. The present disclosure relates to a fusion protein having an amino acid sequence (first amino acid sequence) capable of binding to lipid droplets, and an amino acid sequence (second amino acid sequence) having an enzymatic activity to synthesize a hydrophobic compound, with the enzymatic activity of the second amino acid sequence being maintained.
    Type: Application
    Filed: October 27, 2020
    Publication date: January 5, 2023
    Applicants: Sumitomo Rubber Industries, Ltd., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY, National University Corporation Saitama University
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASI, Toru NAKAYAMA, Satoshi YAMASHITA, Yuzuru TOZAWA
  • Patent number: 11236365
    Abstract: Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.
    Type: Grant
    Filed: July 3, 2019
    Date of Patent: February 1, 2022
    Assignees: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY
    Inventors: Yukino Inoue, Haruhiko Yamaguchi, Kazuhisa Fushihara, Seiji Takahashi, Satoshi Yamashita, Toru Nakayama
  • Publication number: 20220002759
    Abstract: The present invention aims to provide a method for producing a trans-polyisoprenoid which can increase trans rubber production. The present invention is directed to a method for producing a trans-polyisoprenoid in vitro, which involves the use of a gene coding for a trans-prenyltransferase (tPT) family protein and further involves the use of rubber particles bound to a protein encoded by the gene, or a method for producing a trans-polyisoprenoid, which includes introducing into a plant a vector including a promotor having a promoter activity that drives laticifer-specific gene expression and a gene coding for a tPT family protein linked to the promotor to express a protein encoded by the gene specifically in laticifers.
    Type: Application
    Filed: August 26, 2021
    Publication date: January 6, 2022
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY
    Inventors: Yuko SAKURAI, Haruhiko YAMAGUCHI, Yukino INOUE, Kazuhisa FUSHIHARA, Seiji TAKAHASHI, Satoshi YAMASHITA, Toru NAKAYAMA
  • Publication number: 20210395785
    Abstract: The present invention aims to provide a method for producing a trans-polyisoprenoid which can increase trans rubber production. The present invention is directed to a method for producing a trans-polyisoprenoid in vitro, which involves the use of a gene coding for a trans-prenyltransferase (tPT) family protein and further involves the use of rubber particles bound to a protein encoded by the gene, or a method for producing a trans-polyisoprenoid, which includes introducing into a plant a vector including a promotor having a promoter activity that drives laticifer-specific gene expression and a gene coding for a tPT family protein linked to the promotor to express a protein encoded by the gene specifically in laticifers.
    Type: Application
    Filed: August 26, 2021
    Publication date: December 23, 2021
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY
    Inventors: Yuko SAKURAI, Haruhiko YAMAGUCHI, Yukino INOUE, Kazuhisa FUSHIHARA, Seiji TAKAHASHI, Satoshi YAMASHITA, Toru NAKAYAMA
  • Patent number: 11046965
    Abstract: The present invention provides a method which can replace Agrobacterium techniques and which can efficiently produce transformed cells of a rubber-producing plant. The present invention relates to a method of producing transformed cells of a rubber-producing plant, which includes steps a) to e).
    Type: Grant
    Filed: June 12, 2019
    Date of Patent: June 29, 2021
    Assignee: SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventors: Haruhiko Yamaguchi, Yukino Inoue, Teruhiko Terakawa, Tsubasa Yano
  • Patent number: 10920234
    Abstract: Provided is a method that can efficiently produce a transgenic Taraxacum plant in a short period. A method of producing a transgenic Taraxacum plant, including: an infection step of infecting a tissue fragment from a Taraxacum plant with an Agrobacterium tumefaciens containing a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.
    Type: Grant
    Filed: June 7, 2019
    Date of Patent: February 16, 2021
    Assignee: SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventors: Yukino Inoue, Haruhiko Yamaguchi, Teruhiko Terakawa, Tsubasa Yano
  • Patent number: 10907179
    Abstract: The present invention provides a method for producing a polyisoprenoid with which it is possible to enhance the rubber synthesis activity of rubber particles to increase natural rubber production. The present invention relates to a method for producing a polyisoprenoid in vitro, which involves the use of a gene coding for a cis-prenyltransferase (CPT) family protein and a gene coding for a Nogo-B receptor (NgBR) family protein, and further involves the use of rubber particles bound to proteins encoded by these genes; or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a promoter having a promoter activity that drives laticifer-specific gene expression is linked to a gene coding for a CPT family protein and a gene coding for a NgBR family protein, to express proteins encoded by the genes specifically in laticifers.
    Type: Grant
    Filed: June 28, 2016
    Date of Patent: February 2, 2021
    Assignees: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY
    Inventors: Haruhiko Yamaguchi, Yukino Inoue, Kazuhisa Fushihara, Seiji Takahashi, Satoshi Yamashita, Toru Nakayama
  • Patent number: 10889623
    Abstract: The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.
    Type: Grant
    Filed: May 31, 2016
    Date of Patent: January 12, 2021
    Assignees: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY
    Inventors: Haruhiko Yamaguchi, Yukino Inoue, Kazuhisa Fushihara, Seiji Takahashi, Satoshi Yamashita, Toru Nakayama, Yuzuru Tozawa
  • Publication number: 20200377901
    Abstract: To provide methods for producing a natural rubber which enable the production of a natural rubber with an increased molecular weight, and transgenic plants which are able to produce a natural rubber with an increased molecular weight. Included is a method for producing a natural rubber, the method including the steps of: introducing a gene coding for a cis-prenyltransferase (CPT) family protein derived from a rubber-producing plant A into a rubber-producing plant B to prepare a transgenic plant C; and producing a natural rubber using the transgenic plant C, the rubber-producing plant A producing a natural rubber having a higher molecular weight than a natural rubber produced by the rubber-producing plant B not transfected with the gene.
    Type: Application
    Filed: May 19, 2020
    Publication date: December 3, 2020
    Applicant: Sumitomo Rubber Industries, Ltd.
    Inventors: Yuko SAKURAI, Yukino INOUE, Haruhiko YAMAGUCHI, Akari OKADA
  • Publication number: 20200017866
    Abstract: The present invention provides a method which can replace Agrobacterium techniques and which can efficiently produce transformed cells of a rubber-producing plant. The present invention relates to a method of producing transformed cells of a rubber-producing plant, which includes steps a) to e).
    Type: Application
    Filed: June 12, 2019
    Publication date: January 16, 2020
    Applicant: SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Teruhiko TERAKAWA, Tsubasa YANO
  • Publication number: 20200010837
    Abstract: Provided is a method that can efficiently produce a transgenic Taraxacum plant in a short period. A method of producing a transgenic Taraxacum plant, including: an infection step of infecting a tissue fragment from a Taraxacum plant with an Agrobacterium tumefaciens containing a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.
    Type: Application
    Filed: June 7, 2019
    Publication date: January 9, 2020
    Applicant: SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventors: Yukino INOUE, Haruhiko YAMAGUCHI, Teruhiko TERAKAWA, Tsubasa YANO
  • Publication number: 20190376093
    Abstract: The present invention aims to provide a method for producing a trans-polyisoprenoid which can increase trans rubber production. The present invention is directed to a method for producing a trans-polyisoprenoid in vitro, which involves the use of a gene coding for a trans-prenyltransferase (tPT) family protein and further involves the use of rubber particles bound to a protein encoded by the gene, or a method for producing a trans-polyisoprenoid, which includes introducing into a plant a vector including a promoter having a promoter activity that drives laticifer-specific gene expression and a gene coding for a tPT family protein linked to the promoter to express a protein encoded by the gene specifically in laticifers.
    Type: Application
    Filed: November 21, 2017
    Publication date: December 12, 2019
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY
    Inventors: Yuko SAKURAI, Haruhiko YAMAGUCHI, Yukino INOUE, Kazuhisa FUSHIHARA, Seiji TAKAHASHI, Satoshi YAMASHITA, Toru NAKAYAMA
  • Publication number: 20190323039
    Abstract: Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.
    Type: Application
    Filed: July 3, 2019
    Publication date: October 24, 2019
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU University
    Inventors: Yukino INOUE, Haruhiko YAMAGUCHI, Kazuhisa FUSHIHARA, Seiji TAKAHASHI, Satoshi YAMASHITA, Toru NAKAYAMA
  • Patent number: 10420290
    Abstract: A method for cultivating plants of the Asteraceae family, which promotes flower bud formation in the reproductive phase. A method for cultivating plants of the Asteraceae family, wherein the cultivation temperature in the reproductive phase is set lower by at least 5° C. than the cultivation temperature in the vegetative phase to promote flower bud formation in the reproductive phase.
    Type: Grant
    Filed: January 18, 2017
    Date of Patent: September 24, 2019
    Assignee: SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventor: Haruhiko Yamaguchi
  • Patent number: 10415048
    Abstract: The present invention provides methods for enhancing the polyisoprenoid biosynthesis pathway. The present invention further provides isoprenoid-producing plants having an enhanced polyisoprenoid biosynthesis pathway, and methods for producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to methods for regulating the expression of specific protein(s) by a specific transcription factor; isoprenoid-producing plants into which has been introduced a gene encoding a specific transcription factor; and methods for producing a polyisoprenoid using such an isoprenoid-producing plant.
    Type: Grant
    Filed: March 27, 2018
    Date of Patent: September 17, 2019
    Assignee: SUMITOMO RUBBER INDUSTRIES, LTD.
    Inventors: Haruhiko Yamaguchi, Yukino Inoue, Satoshi Kuroda