Abstract: Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.

Abstract: The present invention provides methods for enhancing the polyisoprenoid biosynthesis pathway. The present invention further provides isoprenoid-producing plants having an enhanced polyisoprenoid biosynthesis pathway, and methods for producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to methods for regulating the expression of specific protein(s) by a specific transcription factor; isoprenoid-producing plants into which has been introduced a gene encoding a specific transcription factor; and methods for producing a polyisoprenoid using such an isoprenoid-producing plant.

Abstract: Provided is a method for hydroponic culture of a plant of the family Asteraceae which is suitable for growing the roots of the plant of the family Asteraceae. The method for hydroponic culture of a plant of the family Asteraceae includes a hydroponic culture step including culturing a plant of the family Asteraceae while adjusting the amount of a nutrient solution so that 10 to 50% of the total length from the tip of the tap root of the plant of the family Asteraceae is soaked in the nutrient solution, and the rest of the tap root is exposed to a gas.

Abstract: The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.

Abstract: The present invention provides a method enhancing the polyisoprenoid biosynthesis pathway. Also provided are an isoprenoid-producing plant having an overall enhanced pathway of polyisoprenoid biosynthesis, and a method of producing a polyisoprenoid using the isoprenoid-producing plant. The present invention relates to a method of regulating the expression of hydroxymethylglutaryl-CoA reductase by a bZIP transcription factor.

Abstract: Provided is a method for producing rubber particles with a reduced coagulation tendency. The present invention relates to a method for producing rubber particles with a reduced coagulation tendency, the method including a step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a rubber elongation factor (REF) family protein to bind the REF family protein to the rubber particles.

Abstract: The present invention provides methods for enhancing the polyisoprenoid biosynthesis pathway. The present invention further provides isoprenoid-producing plants having an enhanced polyisoprenoid biosynthesis pathway, and methods for producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to methods for regulating the expression of specific protein(s) by a specific transcription factor; isoprenoid-producing plants into which has been introduced a gene encoding a specific transcription factor; and methods for producing a polyisoprenoid using such an isoprenoid-producing plant.

Abstract: The present invention provides methods for enhancing the polyisoprenoid biosynthesis pathway. The present invention further provides isoprenoid-producing plants having an enhanced polyisoprenoid biosynthesis pathway, and methods for producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to methods for regulating the expression of specific protein(s) by a specific transcription factor; isoprenoid-producing plants into which has been introduced a gene encoding a specific transcription factor; and methods for producing a polyisoprenoid using such an isoprenoid-producing plant.

Abstract: The present invention provides a method for producing a polyisoprenoid with which it is possible to enhance the rubber synthesis activity of rubber particles to increase natural rubber production. The present invention relates to a method for producing a polyisoprenoid in vitro, which involves the use of a gene coding for a cis-prenyltransferase (CPT) family protein and a gene coding for a Nogo-B receptor (NgBR) family protein, and further involves the use of rubber particles bound to proteins encoded by these genes; or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a promoter having a promoter activity that drives laticifer-specific gene expression is linked to a gene coding for a CPT family protein and a gene coding for a NgBR family protein, to express proteins encoded by the genes specifically in laticifers.

Abstract: The present invention provides a method for enhancing the overall pathway of polyisoprenoid biosynthesis. The present invention further provides an isoprenoid-producing plant having an overall enhanced pathway of polyisoprenoid biosynthesis, and a method of producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to a method of regulating by a cytokinin-responsive transcription factor the expression of at least one protein selected from the group consisting of hydroxymethylglutaryl-CoA reductase, isopentenyl diphosphate isomerase, cis-prenyltransferase, and small rubber particle protein.

Abstract: Provided are a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor, which are considered to be involved in polyisoprenoid biosynthesis, into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor, and a method for producing a polyisoprenoid using the transformant. The present invention relates to a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor.

Abstract: The present invention provides methods for enhancing the polyisoprenoid biosynthesis pathway. The present invention further provides isoprenoid-producing plants having an enhanced polyisoprenoid biosynthesis pathway, and methods for producing a polyisoprenoid using such an isoprenoid-producing plant. The present invention relates to methods for regulating the expression of specific protein(s) by a specific transcription factor; isoprenoid-producing plants into which has been introduced a gene encoding a specific transcription factor; and methods for producing a polyisoprenoid using such an isoprenoid-producing plant.

Abstract: The present invention provides a method for enhancing the overall pathway of polyisoprenoid biosynthesis. The present invention further provides an isoprenoid-producing plant having an overall enhanced pathway of polyisoprenoid biosynthesis, and a method for producing polyisoprenoids using such an isoprenoid-producing plant. The present invention relates to a method for regulating by a light-responsive transcription factor the expression of at least one protein selected from the group consisting of hydroxymethylglutaryl-CoA reductase, isopentenyl diphosphate isomerase, cis-prenyltransferase, and small rubber particle protein.

Abstract: The present invention provides an effective method for fractionating rubber particles in natural rubber latex by particle size, and effective methods for transporting or preserving rubber particles. The present invention relates to a method for fractionating rubber particles in natural rubber latex by particle size, the method including centrifuging natural rubber latex in at least four stages at forces of 800 to 3,000×g, 6,500 to 10,000×g, 17,000 to 22,000×g, and 40,000 to 60,000×g to fractionate rubber particles. The present invention also relates to methods for transporting or preserving rubber particles, the methods including the steps of adding a buffer solution and an antioxidant to natural rubber latex or rubber particles fractionated from natural rubber latex; and freezing a mixture prepared in the above step.

Abstract: The present invention provides a method for cultivating plants of the Asteraceae family, which promotes flower bud formation in the reproductive phase. The present invention relates to a method for cultivating plants of the Asteraceae family, wherein the cultivation temperature in the reproductive phase is set lower by at least 5° C. than the cultivation temperature in the vegetative phase to promote flower bud formation in the reproductive phase.

Abstract: The present invention provides a method of increasing production of amorpha-4,11-diene and a method of increasing production of natural rubber. The present invention relates to a method of increasing production of amorpha-4,11-diene which includes the step of attaching an enzyme inhibitor to Arabidopsis thaliana into which has been introduced a gene encoding amorphadiene synthase. The enzyme inhibitor inhibits at least one enzyme other than amorphadiene synthase, that catalyzes an enzymatic reaction in which farnesyl diphosphate acts as a substrate. The present invention also relates to a method of increasing production of natural rubber which includes the step of attaching an enzyme inhibitor to a rubber-producing plant. The enzyme inhibitor inhibits at least one enzyme other than enzymes involved in natural rubber synthesis, that catalyzes an enzymatic reaction in which farnesyl diphosphate acts as a substrate.

Abstract: Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.

Abstract: The present invention provides an effective method for fractionating rubber particles in natural rubber latex by particle size, and effective methods for transporting or preserving rubber particles. The present invention relates to a method for fractionating rubber particles in natural rubber latex by particle size, the method including centrifuging natural rubber latex in at least four stages at forces of 800 to 3,000×g, 6,500 to 10,000×g, 17,000 to 22,000×g, and 40,000 to 60,000×g to fractionate rubber particles. The present invention also relates to methods for transporting or preserving rubber particles, the methods including the steps of adding a buffer solution and an antioxidant to natural rubber latex or rubber particles fractionated from natural rubber latex; and freezing a mixture prepared in the above step.

Abstract: Provided are a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor, which are considered to be involved in polyisoprenoid biosynthesis, into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor, and a method for producing a polyisoprenoid using the transformant. The present invention relates to a transformant produced by introducing a gene coding for a cis-prenyltransferase and a gene coding for a Nogo-B receptor into a host to allow the host to express the cis-prenyltransferase and the Nogo-B receptor.

Abstract: Provided is a method for producing rubber particles with a reduced coagulation tendency. The present invention relates to a method for producing rubber particles with a reduced coagulation tendency, the method including a step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a rubber elongation factor (REF) family protein to bind the REF family protein to the rubber particles.