Abstract: The present invention relates in a first aspect to a method for the purification of biological macromolecular complexes. Typically, no chromatography steps are applied. That is, the present invention relates to a method for the purification of biological macromolecular complexes Furthermore, the present invention relates to a method for crystallization of biological macromolecular complexes comprising the step of purification as described followed by crystallization in a reservoir solution containing a water-soluble polymer. Furthermore, purified biological macromolecular complexes obtainable by the method according to the present invention are provided as well as crystallized biological macromolecular complexes. Finally, a method for determining the suitability of a candidate compound for inhibiting the 20S proteasome of an individual is provided.

Abstract: The present invention relates to a compound of formula (I), wherein X is C?O, C?S or B—OH; Y is an electrophile and Z is a leaving group, or Y?Z is an electrophile; R1 comprises or consists of (a) (i) a first group binding to a proteolytic site of a proteasome, the first group being bound to X; and (ii) optionally a second group enhancing delivery; or (b) a group binding between subunits ?1 and ?2 of a proteasome; R2 and R3 are independently selected from H, methyl, methoxy, ethyl, ethenyl, ethynyl and cyano, wherein methyl and ethyl may be substituted with OH or halogen.

Abstract: In a first aspect, the present invention relates to new recombinant polypeptides derived from the newly identified gamma subunit of the fatty acid synthase protein complex. In addition, a fatty acid synthase protein complex comprising these new recombinant polypeptides are disclosed as well as nucleic acid molecules encoding these polypeptides. Further, a host cells containing the nucleic acid molecule encoding the polypeptides according to the present invention or expressing the polypeptide according to the present invention are described. In addition, an isolated fatty acid synthase protein complex is disclosed containing the newly identified gamma subunit thereof. Moreover, methods for determining the suitability of candidate compounds capable of inhibiting either the ketoreductase, enoylreductase or malonyl/palmitoyl transferase present in the FAS protein complex and methods for designing inhibitors of said enzymes are disclosed.

Abstract: The present invention relates in a first aspect to a method for the purification of biological macromolecular complexes. Typically, no chromatography steps are applied. That is, the present invention relates to a method for the purification of biological macromolecular complexes Furthermore, the present invention relates to a method for crystallization of biological macromolecular complexes comprising the step of purification as described followed by crystallization in a reservoir solution containing a water-soluble polymer. Furthermore, purified biological macromolecular complexes obtainable by the method according to the present invention are provided as well as crystallized biological macromolecular complexes. Finally, a method for determining the suitability of a candidate compound for inhibiting the 20S proteasome of an individual is provided.

Abstract: The present invention relates in a first aspect to a method for the purification of biological macromolecular complexes. Typically, no chromatography steps are applied. That is, the present invention relates to a method for the purification of biological macromolecular complexes Furthermore, the present invention relates to a method for crystallization of biological macromolecular complexes comprising the step of purification as described followed by crystallization in a reservoir solution containing a water-soluble polymer. Furthermore, purified biological macromolecular complexes obtainable by the method according to the present invention are provided as well as crystallized biological macromolecular complexes. Finally, a method for determining the suitability of a candidate compound for inhibiting the 20S proteasome of an individual is provided.

Abstract: The present invention relates to a compound of formula (I), wherein X is C?O, C?S or B—OH; Y is an electrophile and Z is a leaving group, or Y?Z is an electrophile; R1 comprises or consists of (a) (i) a first group binding to a proteolytic site of a proteasome, said first group being bound to X; and (ii) optionally a second group enhancing delivery; or (b) a group binding between subunits ?1 and ?2 of a proteasome; R2 and R3 are independently selected from H, methyl, methoxy, ethyl, ethenyl, ethinyl and cyano, wherein methyl and ethyl maybe substituted with OH or halogen.

Abstract: The present invention relates in a first aspect to a method for the purification of biological macromolecular complexes. Typically, no chromatography steps are applied. That is, the present invention relates to a method for the purification of biological macromolecular complexes Furthermore, the present invention relates to a method for crystallization of biological macromolecular complexes comprising the step of purification as described followed by crystallization in a reservoir solution containing a water-soluble polymer. Furthermore, purified biological macromolecular complexes obtainable by the method according to the present invention are provided as well as crystallized biological macromolecular complexes. Finally, a method for determining the suitability of a candidate compound for inhibiting the 20S proteasome of an individual is provided.

Abstract: The invention relates to a centrifugal pump, in particular a waste water submersible pump having an impeller (1), of which the end face facing the pump intake opening is open and only the end face of the impeller facing away from the intake opening (8) is covered by a circular coaxial impeller hub (2), on which the curved vanes (3, 4) are fastened, and in particular molded on, wherein the base plate (7) having the intake opening (8) facing the impeller (1) or at least one segment (7a) of the base plate is mounted so as to be movable against spring pressure (20) in such a way that the distance thereof from the impeller (1) and thus from the impeller vanes (3, 4) changes.

Abstract: Method, apparatus, and computer program product for providing a cause of a scent and/or sound detected during operation of a vehicle are provided. In the event a vehicle operator hears a sound or smells a scent while operating a vehicle, a microphone can sample the sound and/or an electronic nose can sample the scent. A sound and/or scent profile is generated from the sampled sound and/or sampled scent. The generated sound and/or scent profile are compared to known sound and/or scent profiles stored in a data structure. In the event the generated sound and/or scent profile matches a known sound and/or scent profile, the data structure is checked for correspondences between the matched, known sound and/or scent profile and a cause of the sound and/or scent. In the event a correspondence to a cause exists, then the cause is output to a user to identify the cause of the sound and/or scent.

Abstract: Method, apparatus, and computer program product for providing a cause of a scent and/or sound detected during operation of a vehicle are provided. In the event a vehicle operator hears a sound or smells a scent while operating a vehicle, a microphone can sample the sound and/or an electronic nose can sample the scent. A sound and/or scent profile is generated from the sampled sound and/or sampled scent. The generated sound and/or scent profile are compared to known sound and/or scent profiles stored in a data structure. In the event the generated sound and/or scent profile matches a known sound and/or scent profile, the data structure is checked for correspondences between the matched, known sound and/or scent profile and a cause of the sound and/or scent. In the event a correspondence to a cause exists, then the cause is output to a user to identify the cause of the sound and/or scent.

Abstract: A method for performing high resolution electron microscopy of a soft matter object is described. The method comprises irradiating a soft matter object using an electron microscope having a spherical aberration correction with a substantially constant transfer function in a frequency band of thermal diffuse scattered electrons scattered at the soft matter object. The method comprises detecting the thermal diffuse scattered (TDS) electrons scattered at the soft matter, and using the detected thermal diffuse scattered electrons for deriving therefrom an image of the soft matter object.

Abstract: A fuel cell stack is formed from a plurality of stacked fuel cell units and at least one stack end element. The stacked fuel cell units being surrounded by a housing. A frame element is situated on the at least one stack end element and the housing on the end-face side. At least one seal is situated at least between the stack end element and the housing in the area of the frame element.

Abstract: A fuel cell stack includes a plurality of stacked fuel cell units and at least one stack end element that are clamped a clamping device. The clamped fuel cell units are surrounded by a housing. A circumferential seal, in particular a lip seal, which has recesses for accommodating the clamping device is situated between the housing and the at least one stack end element. The clamping device is electrically insulated at least in the area of the recesses.

Abstract: In a first aspect, provided herein is a method of determining assembly, homogeneity and/or thermodynamic stability of biological macromolecular complexes. In particular, the method allows determining maximum stability of the complexes based on fluorescence changes at an appropriate wavelength as a function of temperature whereby the fluorescence changes reflect the status of assembly, homogeneity and/or thermodynamic stability of the biological macromolecular complexes. The method is based on identifying two state and multistate unfolding fluorescence curves as a function of temperature by fitting the curves according to a multistate unfolding model. In addition, a computer program and a computer program storage medium as well as an apparatus and a system is provided for determining the assembly, homogeneity and/or thermodynamic stability of biological macromolecular complexes.

Abstract: A method for performing high resolution electron microscopy of a soft matter object is described. The method comprises irradiating a soft matter object using an electron microscope having a spherical aberration correction with a substantially constant transfer function in a frequency band of thermal diffuse scattered electrons scattered at the soft matter object. The method comprises detecting the thermal diffuse scattered (TDS) electrons scattered at the soft matter, and using the detected thermal diffuse scattered electrons for deriving therefrom an image of the soft matter object.

Abstract: The invention relates to a centrifugal pump, in particular a waste water submersible pump having an impeller (1), of which the end face facing the pump intake opening is open and only the end face of the impeller facing away from the intake opening (8) is covered by a circular coaxial impeller hub (2), on which the curved vanes (3, 4) are fastened, and in particular moulded on, wherein the base plate (7) having the intake opening (8) facing the impeller (1) or at least one segment (7a) of the base plate is mounted so as to be movable against spring pressure (20) in such a way that the distance thereof from the impeller (1) and thus from the impeller vanes (3, 4) changes.

Abstract: A fuel cell module includes at least one fuel cell stack with a housing. The at least one fuel cell stack is arranged, with a plurality of system components for conducting and/or conditioning the fluids for the supply of the at least one fuel cell stack, with a mechanical interface for fastening the fuel cell module to the vehicle. The mechanical interface is arranged on the housing, wherein the housing forms a carrier for the system components.

Abstract: A fuel cell stack arrangement includes a fuel cells arranged between a first and a second end plate. At least one of the end plates is designed as a channel end plate with at least one channel. The channel has a stack opening, which is opened in the direction of the stack, and a second opening. The stack opening and the second opening are connected to each other via a channel section and are arranged at a distance to each other in a top view looking down on the channel end plate.

Abstract: A fuel cell stack is formed from a plurality of stacked fuel cell units and at least one stack end element. The stacked fuel cell units being surrounded by a housing. A frame element is situated on the at least one stack end element and the housing on the end-face side. At least one seal is situated at least between the stack end element and the housing in the area of the frame element.

Abstract: A fuel cell stack includes a plurality of stacked fuel cell units and at least one stack end element that are clamped a clamping device. The clamped fuel cell units are surrounded by a housing. A circumferential seal, in particular a lip seal, which has recesses for accommodating the clamping device is situated between the housing and the at least one stack end element. The clamping device is electrically insulated at least in the area of the recesses.