Abstract: This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

Abstract: A synthetic diamond material comprising one or more spin defects having a full width half maximum intrinsic inhomogeneous zero phonon line width of no more than 100 MHz. The method for obtain such a material involves a multi-stage annealing process.

Abstract: A molecular delivery system including a plurality of nanowires (e.g., Si NWs), each of the nanowires having a surface layer formed of a silicon-containing material and a covalently bound linker (e.g., silane linker) attached to the surface layer and optionally including a substrate to which the nanowires are adhered or a molecule to be delivered attached to the linker. Also disclosed is a method of delivering into a cell an exogenous molecule.

Abstract: Systems and methods for magnetic sensing and imaging include a sensor having a network of isolated electron-spin quantum bits (qubits) disposed on the surface of the sensor; and a solid state electronic spin system disposed below the surface of the sensor, wherein the solid state electronic spin system has a spin-state dependent fluorescence; a source of light; a source of first external perturbation, wherein the source of first external perturbation generates a magnetic field; a source of second external perturbation; wherein, the source of light and the first and second external perturbations are configured to coherently and independently manipulate the spin states of at least one qubit and at least one solid state electronic spin system; and a detector to optically measure the solid-state electronic spins spin-state dependent fluorescence.

Abstract: A molecular delivery system including a plurality of nanowires (e.g., Si NWs), each of the nanowires having a surface layer formed of a silicon-containing material and a covalently bound linker (e.g., silane linker) attached to the surface layer, and optionally including a substrate to which the nanowires are adhered or a molecule to be delivered attached to the linker. Also disclosed is a method of delivering into a cell an exogenous molecule.

Abstract: An approach to nanoscale thermometry that utilizes coherent manipulation of the electronic spin associated with nitrogen-vacancy (NV) color centers in diamond is disclosed. The methods and apparatus allow for detection of temperature variations down to milli-Kelvin resolution, at nanometer length scales. This biologically compatible approach to thermometry offers superior temperature sensitivity and reproducibility with a reduced measurement time. The disclosed apparatus can be used to study heat-generating intracellular processes.

Abstract: This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences dendritic cell (DC) response(s), for example, dendritic cell maturation, dendritic cell antiviral response(s) and/or dendritic cell inflammatory response(s), as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences dendritic cell response(s) in a variety of therapeutic and/or diagnostic indications.

Abstract: This invention relates generally to compositions and methods for identifying the regulatory network that modulates, controls or otherwise influences T cell balance, for example, Th17 cell differentiation, maintenance and/or function, as well compositions and methods for exploiting the regulatory network that modulates, controls or otherwise influences T cell balance in a variety of therapeutic and/or diagnostic indications. This invention also relates generally to identifying and exploiting target genes and/or target gene products that modulate, control or otherwise influence T cell balance in a variety of therapeutic and/or diagnostic indications.

Abstract: A synthetic diamond material comprising one or more spin defects having a full width half maximum intrinsic inhomogeneous zero phonon line width of no more than 100 MHz. The method for obtain such a material involves a multi-stage annealing process.

Abstract: The present invention generally relates to nanowire arrays and methods of fabricating such arrays. In certain embodiments, the fabrication methods can consistently form NW arrays with reproducible configurations at nanoscale sites and produce NWs of specified heights, diameters, and densities. In some cases, the methods also allow formation of NW arrays containing barriers between regions, which would be prohibitively expensive if prepared by other methods.

Abstract: The present invention generally relates to nanowires and, in some aspects, to methods of using nanowire arrays to identify a therapeutic target for treating a disorder in a subject, identify a treatment for a disorder in a subject, or deliver a biological effector to immune cells. Previous techniques for delivering biological effectors into live immune cells yielded low efficiencies, activated the immune response and induced non-specific inflammation, and/or required harsh conditions that resulted in widespread apoptosis. By contrast, some of the methods described herein are capable of efficiently delivering biomolecular cargo into immune cells, have negligible toxicity, do not activate immune cell function, and/or allow cells to respond appropriately to physiological stimuli.

Abstract: The present invention generally relates to nanowires and, in particular, to multiwell plates comprising nanowires, including systems and methods of making the same. Such multiwell plates can, in some cases, be used in automated equipment or high-throughput applications. For example, a plurality of cells may be placed in at least some of the wells of the multiwell plate, and one or more nanowires may be inserted into at least some of the cells within the wells of the multiwell plate. In some cases, one or more of the nanowires may have coated thereon a biological effector. The cells in each of the wells may be identical or different, and/or the biological effector may the same or different. Such multiwell plates may be used, for example, to test a biological effector against a variety of cell types, or to test a variety of biological effectors against a one or more cell types, or the like.

Abstract: An electrical device including a substrate that has a surface and a plurality of electrically conductive nanowires, each of which has a first end and a second end. Each of the nanowires is formed of a semiconductor, a compound semiconductor, a metal, as or a combination thereof and is coated with an electrically insulating layer except for its first and second ends, the first end being attacked to the surface and the second end being coated with an electrically conductive layer. Also disclosed is a method of sending or receiving an electrical signal to or from a biological cell using the above-described device.

Abstract: A molecular delivery device including a plurality of nanowires (e.g., Si NWs) coated with an electrically conductive layer. Also disclosed are methods for delivering a molecule by nanowire-mediated electroporation.

Abstract: Electrical devices comprised of nanowires are described, along with methods of their manufacture and use. The nanowires can be nanotubes and nanowires. The surface of the nanowires may be selectively functionalized Nanodetector devices are described.

Abstract: A molecular delivery system including a plurality of nanowires (e.g., Si NWs), each of the nanowires having a surface layer formed of a silicon-containing material and a covalently bound linker (e.g., silane linker) attached to the surface layer and optionally including a substrate to which the nanowires are adhered or a molecule to be delivered attached to the linker. Also disclosed is a method of delivering into a cell an exogenous molecule.

Abstract: Electrical devices comprised of nanowires are described, along with methods of their manufacture and use. The nanowires can be nanotubes and nanowires. The surface of the nanowires may be selectively functionalized Nanodetector devices are described.

Abstract: Electrical devices comprised of nanowires are described, along with methods of their manufacture and use. The nanowires can be nanotubes and nanowires. The surface of the nanowires may be selectively functionalized. Nanodetector devices are described.

Abstract: Nanowires are disclosed which comprise transition metal oxides. The transition metal oxides may include oxides of group II, group III, group IV and lanthanide metals. Also disclosed are methods for making nanowires which comprise injecting decomposition agents into a solution comprising solvents and metallic alkoxide or metallic salt precursors.

Abstract: Electrical devices comprised of nanowires are described, along with methods of their manufacture and use. The nanowires can be nanotubes and nanowires. The surface of the nanowires may be selectively functionalized. Nanodetector devices are described.