Abstract: Provided are establishment and suspension acclimation of a Crandell Reese Feline Kidney (CRFK) adherent cell line, and its application. The CRFK cell line, named CRFK-BLA, is deposited at the China General Microbiological Culture Collection Center (CGMCC), with the address being Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, the deposit number being CGMCC NO: 45703; the CRFK cell line was classified and named CRFK cells, and was deposited on Aug. 17, 2023. A serum-free complete suspension culture type CRFK cell line obtained by acclimation on the basis of CRFK-BLA is named CRFK-BLS, and is deposited at the China General Microbiological Culture Collection Center (CGMCC), with the address being Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, the deposit number being CGMCC NO: 45704; the CRFK cell line was classified and named CRFK suspension cells.

Abstract: Provided are establishment and suspension acclimation of a Crandell Reese Feline Kidney (CRFK) adherent cell line, and its application. The CRFK cell line, named CRFK-BLA, is deposited at the China General Microbiological Culture Collection Center (CGMCC), with the address being Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, the deposit number being CGMCC NO: 45703; the CRFK cell line was classified and named CRFK cells, and was deposited on Aug. 17, 2023. A serum-free complete suspension culture type CRFK cell line obtained by acclimation on the basis of CRFK-BLA is named CRFK-BLS, and is deposited at the China General Microbiological Culture Collection Center (CGMCC), with the address being Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, the deposit number being CGMCC NO: 45704; the CRFK cell line was classified and named CRFK suspension cells.

Abstract: One aspect of the present application relates to a recombinant cell comprising a chimeric antigen receptor and shRNA targeted to inhibit the life cycle of HIV. Another aspect of the present application relates to a chimeric antigen molecule, the extracellular region of which is from or comprising the extracellular region of human CD4 molecule, the transmembrane region of which is from or comprising the transmembrane domain of CD8?, and a recombinant cell comprising the above chimeric antigen molecule. The above recombinant cell can be used to treat HIV infection, and have stronger comprehensive killing efficacy, longer effective time, and less risk of causing cytokine storm in the environment of HIV infection.

Abstract: Disclosed is a chain saw and a power tool, including a working part, a body, a brushless motor, a power supply device, a drive circuit, a controller, and a motion detection device. The motion detection device is coupled to the body or the working part for detecting a motion of the body or the working part in at least one direction. The controller is configured to control the drive circuit to make the brushless motor enter a braking mode when the amount of the motion of the body or the working part in at least one direction exceeds a predetermined threshold.

Abstract: A battery pack includes: a battery pack housing; a cell group including multiple cells and accommodated in the battery pack housing; and a monitoring device configured to monitor an ambient temperature at one temperature measurement point or an ambient temperature at each of multiple temperature measurement points in the battery pack housing. A cell includes multiple cell layers, the battery pack further includes a controller configured to control a charging process of the battery pack or a discharging process of the battery pack, the monitoring device is further configured to transmit the monitored ambient temperature to the controller, and the controller is further configured to determine a cell layer temperature of a target cell layer among the multiple cell layers based on at least one ambient temperature and control, based on the cell layer temperature of the target cell layer, the battery pack to be charged or discharge electricity.

Abstract: In response to poor solubility and permeability of conventional voriconazole eye drops and short retention time thereof at the ocular surface, a preparation method of voriconazole hydrogel and eye drops using the same are provided. The preparation method includes steps of: adding DAPA and sodium hydroxide to deionized water, stirring until completely dissolved to produce a DAPA solution; dropping the DAPA solution into triformylbenzene ethanol solution, and reacting at room temperature; then centrifugating, washing precipitation with ethanol, and freeze-drying in vacuum, so as to obtain a polyaldehyde oligomer; and reacting the polyaldehyde oligomer with AHA to synthesize the voriconazole hydrogel. A preparation method of a topical ocular drug includes: applying a therapeutically effective amount the voriconazole hydrogel, wherein the topical ocular drug is for mechanical injury or fungal keratitis. The present invention improves aqueous solubility, penetration, and ocular surface retention time of VCZ.

Abstract: A method for dewatering a coal mine goaf by crossing a neighboring goaf is applicable to a scenario including a first goaf, a second goaf, and a third goaf located sequentially at one side of a roadway. A directional hole mechanism is provided between the roadway and the second goaf as well as between the roadway and the third goaf. The directional hole mechanism passes through a coal seam floor at a bottom of the goaf and is communicated to the second goaf and the third goaf. The directional hole includes a first directional hole and a second directional hole. The first directional hole is communicated to the second goaf, the second directional hole is communicated to the third goaf, and the first directional hole is provided with a casing structure.

Abstract: A pharmaceutical composition of an anti-ANGPTL3 antibody or an antigen binding fragment thereof and its application. Specifically, the pharmaceutical composition comprises an anti-ANGPTL3 antibody or an antigen binding fragment thereof and an acetic acid sodium acetate buffer. The pharmaceutical composition may also comprise a sugar and a surfactant. The pharmaceutical composition has good antibody stability.

Abstract: A display device includes a driving substrate, a plurality of micro light-emitting elements dispersedly disposed on the driving substrate, and a common electrode. A groove is provided on the driving substrate between the micro light-emitting elements. Each of the micro light-emitting elements includes an epitaxial structure layer and a first electrode and a second electrode disposed on opposite sides of the epitaxial structure layer. The common electrode is disposed on the driving substrate, is located between the first electrodes of the micro light-emitting elements, and exposes an upper surface of the first electrode. An insulating layer covers sidewalls of the micro light-emitting elements and extends into the groove to improve reliability of the device.

Abstract: A product identification method comprises instructing an antenna in a product verification area to collect first product identification data of identified products in the case where a user enters the product verification area of a product identification passage; instructing the antenna in the product verification area to collect product verification data of identified products in the case where a user enters a product settlement area of the product identification passage; and determining user product data based on the first product identification data and the product verification data. A product verification area is provided to eliminate a tag outside the passage being falsely read and reduce a tag false reading probability.

Abstract: In view of the problem that the current prevention and treatment manners for corneal neovascularization (CNV) may cause complications, the present disclosure provides an application of CB-839 in preparation of a drug for inhibiting CNV. The CB-839-loaded drug of the present disclosure can significantly inhibit CNV in mice and reduce the infiltration of inflammatory cells without obvious side effects, which makes up for the shortcoming of the prior art.

Abstract: A preparation method of a nanozyme composite-based hybrid hydrogel eye drop includes synthesizing a tannin-coordinated silver/cobalt composite nanoparticle through a coordination reaction of cobalt chloride hexahydrate with ammonia water and an oxidation-reduction reaction of silver nitrate with tannin; and mixing the silver/cobalt composite nanoparticle with an acrylate-modified gelatin, conducting an ultrasonic treatment to allow complete dissolution, and irradiating a resulting mixture under ultraviolet (UV) light to obtain the nanozyme composite-based hybrid hydrogel eye drop. The preparation method of the present disclosure has simple operations and a short preparation time. The eye drop prepared by the preparation method shows a broad-spectrum bactericidal effect against a variety of bacteria and fungi, and has prominent preventive and therapeutic effects for bacterial and drug-resistant bacterial corneal infections.

Abstract: The present disclosure relates to a data verification method, a system, and an edge device, and relates to the field of computer technology. The data verification method includes receiving, by an edge device, cloud data issued by a cloud device and terminal data collected by a terminal device; verifying, by the edge device, the cloud data and the terminal data based on business process logic; and sending, by the edge device, a result of the verifying to at least one of the cloud device or the terminal device.

Abstract: In view of the problem that the current prevention and treatment manners for corneal neovascularization (CNV) may cause complications, the present disclosure provides an application of CB-839 in preparation of a drug for inhibiting CNV. The CB-839-loaded drug of the present disclosure can significantly inhibit CNV in mice and reduce the infiltration of inflammatory cells without obvious side effects, which makes up for the shortcoming of the prior art.

Abstract: A preparation method of a nanozyme composite-based hybrid hydrogel eye drop includes synthesizing a tannin-coordinated silver/cobalt composite nanoparticle through a coordination reaction of cobalt chloride hexahydrate with ammonia water and an oxidation-reduction reaction of silver nitrate with tannin; and mixing the silver/cobalt composite nanoparticle with an acrylate-modified gelatin, conducting an ultrasonic treatment to allow complete dissolution, and irradiating a resulting mixture under ultraviolet (UV) light to obtain the nanozyme composite-based hybrid hydrogel eye drop. The preparation method of the present disclosure has simple operations and a short preparation time. The eye drop prepared by the preparation method shows a broad-spectrum bactericidal effect against a variety of bacteria and fungi, and has prominent preventive and therapeutic effects for bacterial and drug-resistant bacterial corneal infections.

Abstract: Embodiments of the present disclosure provide a method and a device for cooperative control of multiple trains. The method includes: S1, establishing a train dynamic model of urban rail transit; S2, modeling a train control system of urban rail transit based on train-to-train communication; S3, constructing, according to the dynamic model and a control system model, an optimized control target which comprehensively considers distance convergence and speed convergence of train formation; and S4, cooperatively controlling, on the basis of an artificial potential field method and Kalman filtering and according to the optimized control target, the multiple trains. The present disclosure is capable of effectively shortening a train headway.